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The map label for this gene is aspA [H]

Identifier: 150006361

GI number: 150006361

Start: 4783753

End: 4785186

Strand: Reverse

Name: aspA [H]

Synonym: BVU_3880

Alternate gene names: 150006361

Gene position: 4785186-4783753 (Counterclockwise)

Preceding gene: 150006362

Following gene: 150006354

Centisome position: 92.68

GC content: 40.73

Gene sequence:

>1434_bases
ATGGAAACAAAACTTACAGAAGAAACCCGAGTAGAAAGTGATTTAATTGGTGCCCGCGAAATTCCGGCCAGTGCATTGTA
TGGTGTGCAGACTCTAAGAGGCATAGAAAATTTCCCAATAAGTAAATTTCATCTAAATGAATATCCGTTGTTTATCAACG
GTCTTGCCATTACGAAAATGGCAGCTGCAATGGCTAACTATGAGTTAGGTTTATTGACTAAAGAACAAAAAGATGCCATA
GTGTTGGCGTGTCAGGAAATCTTAAATGGTGAGCATCATGAGCAATTCCCGGTAGACATGATTCAGGGAGGTGCCGGTAC
TTCTACCAATATGAATGCTAATGAAGTCATTGCTAACCGTGCTTTGGAGATTATGGGACACAAACGTGGCGAGTATCAAT
ATTGCTCTCCTAATGACCATGTGAACTGCTCGCAATCAACGAATGATGCTTATCCCACTGCAATCCATATTGGCATGTAT
TATTCTCATCTTCGTTTTCTGCCTTATTTGGAATCGCTGATTGGTGCATTCCATAAGAAAGGAGAAGAGTTTGCACATAT
CATCAAAATGGGGCGTACTCAGTTGGAAGATGCCGTTCCTATGACTTTAGGTCAGACATTCAATGGTTTTGCCAGTATAT
TGAGAGATGAAATTCGTCATTTGAATGAAGCTGCGGCAGACTTCCTGACAGTAAATATGGGTGCTACCGCTATTGGTACA
GGCATTTGTGCGGAACCGGGATATGCGGAAAAATGTGTGAAAGCATTGTGTGAAATTACAGGTTTTGATTTCAAATTGTC
GTCAGATTTGGTAGGAGCTACTTCAGATACTTCCTGCTTGGTAGGTTATGCTTCAGCTTTAAAAAGGATAGCCGTAAAGG
TAAATAAAATATGTAATGATTTACGTTTGCTGGCCAGCGGACCGCGTTGTGGCTTAGGAGAATTCAACCTTCCGGCTATG
CAACCGGGATCTTCTATAATGCCAGGTAAGGTGAATCCGGTGATTCCTGAAGTGATGAATCAGATTTGTTATAAGGTAAT
GGGTAACGAACTATGTGTGACTATGGCTGGCGAAGCTGCCCAAATGGAACTGAATGCTATGGAACCGGTGATGGCACAGT
GTTGTTTTGAATCTGTAGATTTAATGATCAATGGTTTTGAAACCTTGCGTACACGTTGTGTAGAAGGTATTACTGCTAAT
GCGGAACGTTGCAAGATTGAAGTGCATAACAGTATTGGTGTAGTAACCGCATTGAATCCGATTATTGGTTATAAAAACTC
TACAAAGATTGCAAAAGAAGCGTTGGAAACAGGTAGAAGTGTTTATGAACTGGTCTTGGAACATGGTATTTTAAATAAGG
AAGAGTTGGATACAATCCTAAGTCCGGAAAACATGTTGAAGCCGGTAAAATTGGATATCAAGCCTAGAAGATAA

Upstream 100 bases:

>100_bases
ATATTTTGAAATTATATCAGATTATATTTTGATATCAAAATGTTAGTTTATACCTTTGCTGTATAAAATAAGAAATGATA
AACTAATAAATAACCTGATC

Downstream 100 bases:

>100_bases
ATTTATTTCACCACAGACTACAGGAAGGTAAAGAACTTGTTTCATGAAGCCGGCTTCAATATTTGTAAAGCCGGCTCCAT
GAGTGGTGGCACCGGTTTTA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
METKLTEETRVESDLIGAREIPASALYGVQTLRGIENFPISKFHLNEYPLFINGLAITKMAAAMANYELGLLTKEQKDAI
VLACQEILNGEHHEQFPVDMIQGGAGTSTNMNANEVIANRALEIMGHKRGEYQYCSPNDHVNCSQSTNDAYPTAIHIGMY
YSHLRFLPYLESLIGAFHKKGEEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILRDEIRHLNEAAADFLTVNMGATAIGT
GICAEPGYAEKCVKALCEITGFDFKLSSDLVGATSDTSCLVGYASALKRIAVKVNKICNDLRLLASGPRCGLGEFNLPAM
QPGSSIMPGKVNPVIPEVMNQICYKVMGNELCVTMAGEAAQMELNAMEPVMAQCCFESVDLMINGFETLRTRCVEGITAN
AERCKIEVHNSIGVVTALNPIIGYKNSTKIAKEALETGRSVYELVLEHGILNKEELDTILSPENMLKPVKLDIKPRR

Sequences:

>Translated_477_residues
METKLTEETRVESDLIGAREIPASALYGVQTLRGIENFPISKFHLNEYPLFINGLAITKMAAAMANYELGLLTKEQKDAI
VLACQEILNGEHHEQFPVDMIQGGAGTSTNMNANEVIANRALEIMGHKRGEYQYCSPNDHVNCSQSTNDAYPTAIHIGMY
YSHLRFLPYLESLIGAFHKKGEEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILRDEIRHLNEAAADFLTVNMGATAIGT
GICAEPGYAEKCVKALCEITGFDFKLSSDLVGATSDTSCLVGYASALKRIAVKVNKICNDLRLLASGPRCGLGEFNLPAM
QPGSSIMPGKVNPVIPEVMNQICYKVMGNELCVTMAGEAAQMELNAMEPVMAQCCFESVDLMINGFETLRTRCVEGITAN
AERCKIEVHNSIGVVTALNPIIGYKNSTKIAKEALETGRSVYELVLEHGILNKEELDTILSPENMLKPVKLDIKPRR
>Mature_477_residues
METKLTEETRVESDLIGAREIPASALYGVQTLRGIENFPISKFHLNEYPLFINGLAITKMAAAMANYELGLLTKEQKDAI
VLACQEILNGEHHEQFPVDMIQGGAGTSTNMNANEVIANRALEIMGHKRGEYQYCSPNDHVNCSQSTNDAYPTAIHIGMY
YSHLRFLPYLESLIGAFHKKGEEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILRDEIRHLNEAAADFLTVNMGATAIGT
GICAEPGYAEKCVKALCEITGFDFKLSSDLVGATSDTSCLVGYASALKRIAVKVNKICNDLRLLASGPRCGLGEFNLPAM
QPGSSIMPGKVNPVIPEVMNQICYKVMGNELCVTMAGEAAQMELNAMEPVMAQCCFESVDLMINGFETLRTRCVEGITAN
AERCKIEVHNSIGVVTALNPIIGYKNSTKIAKEALETGRSVYELVLEHGILNKEELDTILSPENMLKPVKLDIKPRR

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=40.6521739130435, Blast_Score=329, Evalue=4e-90,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=54.1935483870968, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1787896, Length=462, Percent_Identity=40.4761904761905, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=41.9565217391304, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=41.1042944785276, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324993, Length=467, Percent_Identity=39.6145610278373, Blast_Score=346, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24640177, Length=465, Percent_Identity=40.8602150537634, Blast_Score=351, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24640179, Length=465, Percent_Identity=40.8602150537634, Blast_Score=351, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24662684, Length=471, Percent_Identity=40.1273885350318, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=41.6846652267819, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24583245, Length=467, Percent_Identity=38.7580299785867, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52223; Mature: 52223

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKLTEETRVESDLIGAREIPASALYGVQTLRGIENFPISKFHLNEYPLFINGLAITKM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEHHHHHHH
AAAMANYELGLLTKEQKDAIVLACQEILNGEHHEQFPVDMIQGGAGTSTNMNANEVIANR
HHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
ALEIMGHKRGEYQYCSPNDHVNCSQSTNDAYPTAIHIGMYYSHLRFLPYLESLIGAFHKK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
GEEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILRDEIRHLNEAAADFLTVNMGATAIGT
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHCC
GICAEPGYAEKCVKALCEITGFDFKLSSDLVGATSDTSCLVGYASALKRIAVKVNKICND
CCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRLLASGPRCGLGEFNLPAMQPGSSIMPGKVNPVIPEVMNQICYKVMGNELCVTMAGEAA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHH
QMELNAMEPVMAQCCFESVDLMINGFETLRTRCVEGITANAERCKIEVHNSIGVVTALNP
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHEEEEECCCCCCHHHHHH
IIGYKNSTKIAKEALETGRSVYELVLEHGILNKEELDTILSPENMLKPVKLDIKPRR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCEEECCCCCC
>Mature Secondary Structure
METKLTEETRVESDLIGAREIPASALYGVQTLRGIENFPISKFHLNEYPLFINGLAITKM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEHHHHHHH
AAAMANYELGLLTKEQKDAIVLACQEILNGEHHEQFPVDMIQGGAGTSTNMNANEVIANR
HHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
ALEIMGHKRGEYQYCSPNDHVNCSQSTNDAYPTAIHIGMYYSHLRFLPYLESLIGAFHKK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
GEEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILRDEIRHLNEAAADFLTVNMGATAIGT
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHCC
GICAEPGYAEKCVKALCEITGFDFKLSSDLVGATSDTSCLVGYASALKRIAVKVNKICND
CCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRLLASGPRCGLGEFNLPAMQPGSSIMPGKVNPVIPEVMNQICYKVMGNELCVTMAGEAA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHH
QMELNAMEPVMAQCCFESVDLMINGFETLRTRCVEGITANAERCKIEVHNSIGVVTALNP
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHEEEEECCCCCCHHHHHH
IIGYKNSTKIAKEALETGRSVYELVLEHGILNKEELDTILSPENMLKPVKLDIKPRR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]