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The map label for this gene is lacZ [H]

Identifier: 150006328

GI number: 150006328

Start: 4746758

End: 4748797

Strand: Reverse

Name: lacZ [H]

Synonym: BVU_3844

Alternate gene names: 150006328

Gene position: 4748797-4746758 (Counterclockwise)

Preceding gene: 150006329

Following gene: 150006326

Centisome position: 91.97

GC content: 42.94

Gene sequence:

>2040_bases
ATGAAAAACCTCAGAAGAACTTTCACCGTATTATTTGCCGCAGCTTTCAGCATGCAGGTGTTGGCACAAAGAGAAGATAA
GCTTATCAATCAGGATTGGAGTTTCCGTTTTTCCCATCAAGTAAATGCTAGTGCTGCACGTAGGGTAGACTTGCCTCATA
CTTGGAATGCGCAGGATGCCTTGGGTGGTAAGCACGATTACAAGCGGGGAATAGGTAATTATACTAAAAAGATATTCATC
CGTCCCGAGTGGCAAGGCAAGCGGCTTTTTTTACGTTTCGAGGGGGCGAATTGCGTGAGCAACGTCTTTGTAAATGGTAA
ACATATAGGTGAGCATCGGGGAGGCTATGGAGCTTTTGTCTTCGAGATAACCGATAAAGTGGAATATGGAAAAGAGAATA
CATTGCTGGTACGTGTCAATAATGGCGAGCAGTTGGATGTAATGCCATTGGTCGGTGATTTTAATTTTTATGGCGGCATT
TATCGGGATGTACATCTGTTGCTGACTGATAACCTGTGTATTTCTCCGTTAGATTATGCATCGTCTGGGGTTTATCTCAT
ACAGCAACAGGTTACGGATAAACAGGCAACTATTTGTGCACGCGTAAATCTTTCTAATGGAACGGGAGAACTCCGGAAAG
TAGTGCTCCGGTTGCAGGTTAATGACGGGAAAAAGACTGTGTATGAAACGGAGAAGGAAGTAAGTATGATTCCTCATACA
GATGTACAGGTAGAGAATATAGAGTTCATACTGAAGAATCCGCGTCTTTGGAACGGTATGCAGGATCCGTTCATGTATCA
GGCGGTGGTGACTTTGATTAAAGACGGAAAAGAATTGGACAAAGTGGAACAGCCGTTAGGGTTACGTTATTACGTTACGG
ATCCCGATAAAGGTTTTTTCTTGAATGGAAAATACTTGCCGCTGCATGGTGTATGCCGTCATCAGGAGAGGGCGGAAGTG
GGTAATGCTCTTTGTCCGGTGCATCACGAGGAAGATACTCGTATCATGCTTGATATGGGGGTGAATGCTGTTCGTCTGGC
ACATTATCCGCAAGCTACCTATATGTATGATTTAATGGATAAACATGGTATTGTTACTTGGGCTGAGATTCCATTTGTGG
GGCCAGGAGGATACGCTGATAAAGGTTTTGTTGATCAGCCTTCTTTCCGTGAAAATGGTAAAGAGCAGTTGAAAGAGATG
ATACGCCAGCATTACAATCATCCTAGTATCTGTTTTTGGGGGTTGTTCAATGAATTAAAAGAGCAGGGAGACAATCCTGT
AGAGTATATAAAGGAGTTGAATGCTATAGCGCATCAAGAGGATCCTACTCGTCCTACTACTTCTGCAAGTAATCAGGAGG
GTGCGTTGAATTTCATTACTGATCATATAGCTTGGAATCGCTATGACGGATGGTATGGGGCTACGCCGGCTACGCTTGCC
ACATGGTTGGATGCTACTCATAAGAACCATCCCGAAATGAAAATTGCCATCAGCGAATATGGTGCGGGTGCTAGTATCTA
TCATCAGCAGGATTCATTGGTACAAACTGTTCCCGGAAGTTGGTGGCATCCAGAGAATTGGCAAACAGAATATCACATTC
AGAATTGGAAAATAATAAACGAACGTCCGTATGTGTGGGCTAGTTTTGTGTGGAATATGTTTGATTTCGGTGCGGCTCAT
CGTACGGAAGGTGATCGTCCGGGCATTAATGACAAAGGGCTGGTAACCCATGATCGCAAGATTAAAAAGGATGCTTATTA
TTTTTATCGGGCCAATTGGAATCCGGAACCTATGATTTATATCGCGGGGCGTCGTAATGTGAATCGAGTGAAGCCACTCG
TTGATGTACAGGTTTTTTCGAATGTAGAAGAAGTGATATTGATCGTGAATGACTGTCAGTGTGGAAAGATGAAACCAGAC
AGTTTGAAAGTTTGTTTGTTCAAGGATGTCCCTCTCAGAAAAGGAAGGAATGAAATAGAAGTACGTGCCAATGATAGTAA
AAAACAATTGATAGATCGATGTACTTGGATATTACAATAA

Upstream 100 bases:

>100_bases
TTCGTATTCAACTATTCTTCTGTGTTTTAGTTGCTCTCTCAAGTTTTTTGCCTATTTTTGCTGTTGGAACATAAATCAAA
GTATAATAATAAAAATACAT

Downstream 100 bases:

>100_bases
AACAATTGTCGCAGGGAGTTATACATTTCTTTCTGCGGCGATTGTTTTTTATTAGTTTGATTTAACCAGTTGGCTCATCG
TCTTGTGTTGATTCTACATA

Product: beta-glycosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MKNLRRTFTVLFAAAFSMQVLAQREDKLINQDWSFRFSHQVNASAARRVDLPHTWNAQDALGGKHDYKRGIGNYTKKIFI
RPEWQGKRLFLRFEGANCVSNVFVNGKHIGEHRGGYGAFVFEITDKVEYGKENTLLVRVNNGEQLDVMPLVGDFNFYGGI
YRDVHLLLTDNLCISPLDYASSGVYLIQQQVTDKQATICARVNLSNGTGELRKVVLRLQVNDGKKTVYETEKEVSMIPHT
DVQVENIEFILKNPRLWNGMQDPFMYQAVVTLIKDGKELDKVEQPLGLRYYVTDPDKGFFLNGKYLPLHGVCRHQERAEV
GNALCPVHHEEDTRIMLDMGVNAVRLAHYPQATYMYDLMDKHGIVTWAEIPFVGPGGYADKGFVDQPSFRENGKEQLKEM
IRQHYNHPSICFWGLFNELKEQGDNPVEYIKELNAIAHQEDPTRPTTSASNQEGALNFITDHIAWNRYDGWYGATPATLA
TWLDATHKNHPEMKIAISEYGAGASIYHQQDSLVQTVPGSWWHPENWQTEYHIQNWKIINERPYVWASFVWNMFDFGAAH
RTEGDRPGINDKGLVTHDRKIKKDAYYFYRANWNPEPMIYIAGRRNVNRVKPLVDVQVFSNVEEVILIVNDCQCGKMKPD
SLKVCLFKDVPLRKGRNEIEVRANDSKKQLIDRCTWILQ

Sequences:

>Translated_679_residues
MKNLRRTFTVLFAAAFSMQVLAQREDKLINQDWSFRFSHQVNASAARRVDLPHTWNAQDALGGKHDYKRGIGNYTKKIFI
RPEWQGKRLFLRFEGANCVSNVFVNGKHIGEHRGGYGAFVFEITDKVEYGKENTLLVRVNNGEQLDVMPLVGDFNFYGGI
YRDVHLLLTDNLCISPLDYASSGVYLIQQQVTDKQATICARVNLSNGTGELRKVVLRLQVNDGKKTVYETEKEVSMIPHT
DVQVENIEFILKNPRLWNGMQDPFMYQAVVTLIKDGKELDKVEQPLGLRYYVTDPDKGFFLNGKYLPLHGVCRHQERAEV
GNALCPVHHEEDTRIMLDMGVNAVRLAHYPQATYMYDLMDKHGIVTWAEIPFVGPGGYADKGFVDQPSFRENGKEQLKEM
IRQHYNHPSICFWGLFNELKEQGDNPVEYIKELNAIAHQEDPTRPTTSASNQEGALNFITDHIAWNRYDGWYGATPATLA
TWLDATHKNHPEMKIAISEYGAGASIYHQQDSLVQTVPGSWWHPENWQTEYHIQNWKIINERPYVWASFVWNMFDFGAAH
RTEGDRPGINDKGLVTHDRKIKKDAYYFYRANWNPEPMIYIAGRRNVNRVKPLVDVQVFSNVEEVILIVNDCQCGKMKPD
SLKVCLFKDVPLRKGRNEIEVRANDSKKQLIDRCTWILQ
>Mature_679_residues
MKNLRRTFTVLFAAAFSMQVLAQREDKLINQDWSFRFSHQVNASAARRVDLPHTWNAQDALGGKHDYKRGIGNYTKKIFI
RPEWQGKRLFLRFEGANCVSNVFVNGKHIGEHRGGYGAFVFEITDKVEYGKENTLLVRVNNGEQLDVMPLVGDFNFYGGI
YRDVHLLLTDNLCISPLDYASSGVYLIQQQVTDKQATICARVNLSNGTGELRKVVLRLQVNDGKKTVYETEKEVSMIPHT
DVQVENIEFILKNPRLWNGMQDPFMYQAVVTLIKDGKELDKVEQPLGLRYYVTDPDKGFFLNGKYLPLHGVCRHQERAEV
GNALCPVHHEEDTRIMLDMGVNAVRLAHYPQATYMYDLMDKHGIVTWAEIPFVGPGGYADKGFVDQPSFRENGKEQLKEM
IRQHYNHPSICFWGLFNELKEQGDNPVEYIKELNAIAHQEDPTRPTTSASNQEGALNFITDHIAWNRYDGWYGATPATLA
TWLDATHKNHPEMKIAISEYGAGASIYHQQDSLVQTVPGSWWHPENWQTEYHIQNWKIINERPYVWASFVWNMFDFGAAH
RTEGDRPGINDKGLVTHDRKIKKDAYYFYRANWNPEPMIYIAGRRNVNRVKPLVDVQVFSNVEEVILIVNDCQCGKMKPD
SLKVCLFKDVPLRKGRNEIEVRANDSKKQLIDRCTWILQ

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=557, Percent_Identity=24.4165170556553, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1787903, Length=624, Percent_Identity=25.9615384615385, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI48994920, Length=363, Percent_Identity=29.2011019283747, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1786539, Length=387, Percent_Identity=24.8062015503876, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17510825, Length=584, Percent_Identity=26.541095890411, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI62471735, Length=557, Percent_Identity=26.032315978456, Blast_Score=139, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922582, Length=557, Percent_Identity=26.032315978456, Blast_Score=139, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24655438, Length=557, Percent_Identity=26.032315978456, Blast_Score=139, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24651745, Length=563, Percent_Identity=26.6429840142096, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 78169; Mature: 78169

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLRRTFTVLFAAAFSMQVLAQREDKLINQDWSFRFSHQVNASAARRVDLPHTWNAQDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHEECCCCCCCCHHC
LGGKHDYKRGIGNYTKKIFIRPEWQGKRLFLRFEGANCVSNVFVNGKHIGEHRGGYGAFV
CCCCHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEHEEECCEECCCCCCCCEEEE
FEITDKVEYGKENTLLVRVNNGEQLDVMPLVGDFNFYGGIYRDVHLLLTDNLCISPLDYA
EEEECHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCHHHEEEEEEECCCEECCCCCC
SSGVYLIQQQVTDKQATICARVNLSNGTGELRKVVLRLQVNDGKKTVYETEKEVSMIPHT
CCCEEEEEEHHCCCCEEEEEEEEECCCCHHHEEEEEEEEECCCCCHHHCCCHHHEECCCC
DVQVENIEFILKNPRLWNGMQDPFMYQAVVTLIKDGKELDKVEQPLGLRYYVTDPDKGFF
CCEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCEE
LNGKYLPLHGVCRHQERAEVGNALCPVHHEEDTRIMLDMGVNAVRLAHYPQATYMYDLMD
ECCEECCHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHH
KHGIVTWAEIPFVGPGGYADKGFVDQPSFRENGKEQLKEMIRQHYNHPSICFWGLFNELK
CCCEEEEEECCEECCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
EQGDNPVEYIKELNAIAHQEDPTRPTTSASNQEGALNFITDHIAWNRYDGWYGATPATLA
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCCHHHHH
TWLDATHKNHPEMKIAISEYGAGASIYHQQDSLVQTVPGSWWHPENWQTEYHIQNWKIIN
HHHHHHCCCCCCEEEEEECCCCCCEEEECCCCHHHHCCCCCCCCCCCCEEEEEECEEEEC
ERPYVWASFVWNMFDFGAAHRTEGDRPGINDKGLVTHDRKIKKDAYYFYRANWNPEPMIY
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCEEE
IAGRRNVNRVKPLVDVQVFSNVEEVILIVNDCQCGKMKPDSLKVCLFKDVPLRKGRNEIE
ECCCCCCCHHCCEEEHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEE
VRANDSKKQLIDRCTWILQ
EEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKNLRRTFTVLFAAAFSMQVLAQREDKLINQDWSFRFSHQVNASAARRVDLPHTWNAQDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHEECCCCCCCCHHC
LGGKHDYKRGIGNYTKKIFIRPEWQGKRLFLRFEGANCVSNVFVNGKHIGEHRGGYGAFV
CCCCHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEHEEECCEECCCCCCCCEEEE
FEITDKVEYGKENTLLVRVNNGEQLDVMPLVGDFNFYGGIYRDVHLLLTDNLCISPLDYA
EEEECHHHCCCCCEEEEEECCCCEEEEEEEEECCCCCCCHHHEEEEEEECCCEECCCCCC
SSGVYLIQQQVTDKQATICARVNLSNGTGELRKVVLRLQVNDGKKTVYETEKEVSMIPHT
CCCEEEEEEHHCCCCEEEEEEEEECCCCHHHEEEEEEEEECCCCCHHHCCCHHHEECCCC
DVQVENIEFILKNPRLWNGMQDPFMYQAVVTLIKDGKELDKVEQPLGLRYYVTDPDKGFF
CCEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCEE
LNGKYLPLHGVCRHQERAEVGNALCPVHHEEDTRIMLDMGVNAVRLAHYPQATYMYDLMD
ECCEECCHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHH
KHGIVTWAEIPFVGPGGYADKGFVDQPSFRENGKEQLKEMIRQHYNHPSICFWGLFNELK
CCCEEEEEECCEECCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
EQGDNPVEYIKELNAIAHQEDPTRPTTSASNQEGALNFITDHIAWNRYDGWYGATPATLA
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCCHHHHH
TWLDATHKNHPEMKIAISEYGAGASIYHQQDSLVQTVPGSWWHPENWQTEYHIQNWKIIN
HHHHHHCCCCCCEEEEEECCCCCCEEEECCCCHHHHCCCCCCCCCCCCEEEEEECEEEEC
ERPYVWASFVWNMFDFGAAHRTEGDRPGINDKGLVTHDRKIKKDAYYFYRANWNPEPMIY
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCEEE
IAGRRNVNRVKPLVDVQVFSNVEEVILIVNDCQCGKMKPDSLKVCLFKDVPLRKGRNEIE
ECCCCCCCHHCCEEEHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEE
VRANDSKKQLIDRCTWILQ
EEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA