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The map label for this gene is capG [H]

Identifier: 150005949

GI number: 150005949

Start: 4295518

End: 4296252

Strand: Reverse

Name: capG [H]

Synonym: BVU_3447

Alternate gene names: 150005949

Gene position: 4296252-4295518 (Counterclockwise)

Preceding gene: 150005950

Following gene: 150005948

Centisome position: 83.21

GC content: 36.6

Gene sequence:

>735_bases
ATGATAAAATTATCATCTATAACAGCGCATATATTAGATATTTGGCATCGCCGAAACAGCAGATCGTACATTGCTCATCT
GCGTTCACTGGGTATAAGAATTGGTGATGGTTGCATTTTTAGAGATCCGCTGACTACGAGAATCGATGTTTCAAGGCCAG
CCTTGGTTTCCATTGGTAGTAATGTTGATATGAATACATATTTCCAGATATTGACGCATGATTGGGCTAGCTTTGTTTTT
AGGAATAAATATCATGATTTTGTGAATTCTTCAGGAAGAGTGGAAATAGGTTCGAATATTTATATTGGCACAAATGTCAT
TGTATTACGTGGTGTTACTATTGGTGATAACTGTGTTATAGGTGCTGGCAGTGTTGTAACACATGATATCCCTGCCAATA
GTGTAGCGGTAGGAGCTCCTTGTCGTGTAGTGTGTTCATTAGATGAATATTATCAAAAACGGAAAGTCAAAGGATTGCAG
GAAGCTGTGGAACATGTTAAGGCTTTTCAAAAGAATTTTGGTCGAGATCCTTTGCCTCATGAATTATATGAAGAATTCAT
CTATTTTGTAGATGCCTCAAATGTAGAGGAATATGAACGTCAAGGTGTGCCTGTTCGGTCTCAGTTAAGTATAGCTTATG
GTGATTGGTTATCTACTCATAAGGCGAAATTTTCGTCTTATGATGATTTTATTCAATATGTAAATCAAAAAATGAATGAA
ACAGCCGAAAGTTAG

Upstream 100 bases:

>100_bases
ATGGATAAAAATTCGTGTATAATTTATAGGCAATGTTCAAATGCTCTAAGTAACATATTAATAGGTTTTTATATTAAATA
TTCATTGTACAATTCAATAT

Downstream 100 bases:

>100_bases
TATAATAGTCCCTGTTTATAATGTGGAGAAATATCTTGATCGTTGCATGCAATCGCTTCTTGGGCAAACGCTGAAAGAAT
TAGAAATTATTCTAGTGGAT

Product: putative acetyl transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIKLSSITAHILDIWHRRNSRSYIAHLRSLGIRIGDGCIFRDPLTTRIDVSRPALVSIGSNVDMNTYFQILTHDWASFVF
RNKYHDFVNSSGRVEIGSNIYIGTNVIVLRGVTIGDNCVIGAGSVVTHDIPANSVAVGAPCRVVCSLDEYYQKRKVKGLQ
EAVEHVKAFQKNFGRDPLPHELYEEFIYFVDASNVEEYERQGVPVRSQLSIAYGDWLSTHKAKFSSYDDFIQYVNQKMNE
TAES

Sequences:

>Translated_244_residues
MIKLSSITAHILDIWHRRNSRSYIAHLRSLGIRIGDGCIFRDPLTTRIDVSRPALVSIGSNVDMNTYFQILTHDWASFVF
RNKYHDFVNSSGRVEIGSNIYIGTNVIVLRGVTIGDNCVIGAGSVVTHDIPANSVAVGAPCRVVCSLDEYYQKRKVKGLQ
EAVEHVKAFQKNFGRDPLPHELYEEFIYFVDASNVEEYERQGVPVRSQLSIAYGDWLSTHKAKFSSYDDFIQYVNQKMNE
TAES
>Mature_244_residues
MIKLSSITAHILDIWHRRNSRSYIAHLRSLGIRIGDGCIFRDPLTTRIDVSRPALVSIGSNVDMNTYFQILTHDWASFVF
RNKYHDFVNSSGRVEIGSNIYIGTNVIVLRGVTIGDNCVIGAGSVVTHDIPANSVAVGAPCRVVCSLDEYYQKRKVKGLQ
EAVEHVKAFQKNFGRDPLPHELYEEFIYFVDASNVEEYERQGVPVRSQLSIAYGDWLSTHKAKFSSYDDFIQYVNQKMNE
TAES

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=69, Percent_Identity=50.7246376811594, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 27614; Mature: 27614

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLSSITAHILDIWHRRNSRSYIAHLRSLGIRIGDGCIFRDPLTTRIDVSRPALVSIGS
CEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCEEECCCCCCEEECCCCEEEEECC
NVDMNTYFQILTHDWASFVFRNKYHDFVNSSGRVEIGSNIYIGTNVIVLRGVTIGDNCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCEEEEEEEEECCCEEE
GAGSVVTHDIPANSVAVGAPCRVVCSLDEYYQKRKVKGLQEAVEHVKAFQKNFGRDPLPH
ECCCEEEECCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ELYEEFIYFVDASNVEEYERQGVPVRSQLSIAYGDWLSTHKAKFSSYDDFIQYVNQKMNE
HHHHHHHHHHCCCCHHHHHHCCCCCHHHEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAES
HCCC
>Mature Secondary Structure
MIKLSSITAHILDIWHRRNSRSYIAHLRSLGIRIGDGCIFRDPLTTRIDVSRPALVSIGS
CEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCEEECCCCCCEEECCCCEEEEECC
NVDMNTYFQILTHDWASFVFRNKYHDFVNSSGRVEIGSNIYIGTNVIVLRGVTIGDNCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCEEEEEEEEECCCEEE
GAGSVVTHDIPANSVAVGAPCRVVCSLDEYYQKRKVKGLQEAVEHVKAFQKNFGRDPLPH
ECCCEEEECCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ELYEEFIYFVDASNVEEYERQGVPVRSQLSIAYGDWLSTHKAKFSSYDDFIQYVNQKMNE
HHHHHHHHHHCCCCHHHHHHCCCCCHHHEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TAES
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]