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The map label for this gene is prrB [H]

Identifier: 150005916

GI number: 150005916

Start: 4256679

End: 4257971

Strand: Reverse

Name: prrB [H]

Synonym: BVU_3412

Alternate gene names: 150005916

Gene position: 4257971-4256679 (Counterclockwise)

Preceding gene: 150005917

Following gene: 150005915

Centisome position: 82.47

GC content: 35.65

Gene sequence:

>1293_bases
ATGGAGAAATATAATAGCTATAAAGATAGTGGTATACAATGGCTTGGTAAAATTCCGAGTCATTGGGAAATTAAACGCTC
TAGATTAATATTTGATGAAAATGTTGAGACAAATTCTACATGTAATAATACTAATCAACTTCAATTTAGATTTGGAACGA
TTGAACCTAAGAAATCTCAAGAAATGGATTCTGATTTAAAGAAAATCATTTCAAAGTACACCATAGTTCAAAATGGAGAT
ATTATGATCAATGGGCTTAATCTGAATTATGATTTTGTATCTCAAAGAGTTGCTCAAGTAAAAGAAAAGGGAATCATTAC
TTCTGCTTACATAGCCCTACGCCCGAAAGAAAATATATGTTCAGATTATTTTACTTATCTTCTAAAAGGAATGGATGCAC
GAAAAGTGTTTCATGGAATGGGATGTGGTGTCAGACTCACATTATCTTTTAAAGAATTTAGAAATGAGCTATTACCTATC
CCTCCTTTAGAAGAACAACAATCCATGGCTACCTATCTCGACAAGGCTACTGCCGAAATAGACAAAGCCATTGCCCAACA
GCAGCGTATGATTGATTTGCTTAATGAAAGAAAGCAAATCATCATTCAGCGTGCAGTTACGAAAGGGCTGGACGGGAATG
TGGAAATGAAGAATTCAGGATTGAATTGGTTGGGACAAATACCATCACATTGGGAATCTTTGCCGTTAACCTATGTGTTT
GAAATGCGAAATGGATATACACCATCTAAAAACGATCCTACATATTGGACTAATGGTTCAATACCATGGTATCGTATGGA
GGATATAAGAAAATCTGGGCGCTTTCTAAGGGAGGCAATGCAATACGTTACTACCAAAGCCATAAACGGAAAAGGCACCT
TTAAAGCAGGATCATACATCATGGCAATCTGTACAGCGTCAATAGGTGAACATGCGATGTTAATTGCTGATTCGCTTGCG
AATCAGCGATTTGCGAATTTTAAAATTCGCAAATCGCTGATTGAATCTTTCTACCCGCTTTTCCTGTTCTACTATATGTA
TGTAGTTGGTGATTTTTGTCGTGAAAACTCAAATTCTACTTGCTTTCAATATGTAGATATGGGAGCATTAAAACGTTTCC
CCATACCTAAACCATCGATGGAAGAACAAAAGAATATAGTGAGTTCCTTAACTCAGAATTTACAACAAATAAACACAGCT
TTAGAACGGATACAAAAACAGATAACTCTCCTTCAAGAGCGCAAGCAAATTATCATCAGTGAAGTAGTAACGGGTAAAAT
AAAAGTATCATAA

Upstream 100 bases:

>100_bases
GCTCAACTTCGCTCTTTGGAAGAGAATGAACATGATATTCTGGAGTTAGATCGCCAGAGTCAAGGGTTTATCGAAGCATT
GCTTAAATTGATGTAGGCTT

Downstream 100 bases:

>100_bases
CATGGCAACAAAAAATATAGAACCGAATTTACGGCTTATCAGTGAATATACCAAGTTGGGAAAAGATGACAGATTCCGTA
TCCCGGAATACCAAAGAGGC

Product: type I restriction-modification system S subunit

Products: NA

Alternate protein names: S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MEKYNSYKDSGIQWLGKIPSHWEIKRSRLIFDENVETNSTCNNTNQLQFRFGTIEPKKSQEMDSDLKKIISKYTIVQNGD
IMINGLNLNYDFVSQRVAQVKEKGIITSAYIALRPKENICSDYFTYLLKGMDARKVFHGMGCGVRLTLSFKEFRNELLPI
PPLEEQQSMATYLDKATAEIDKAIAQQQRMIDLLNERKQIIIQRAVTKGLDGNVEMKNSGLNWLGQIPSHWESLPLTYVF
EMRNGYTPSKNDPTYWTNGSIPWYRMEDIRKSGRFLREAMQYVTTKAINGKGTFKAGSYIMAICTASIGEHAMLIADSLA
NQRFANFKIRKSLIESFYPLFLFYYMYVVGDFCRENSNSTCFQYVDMGALKRFPIPKPSMEEQKNIVSSLTQNLQQINTA
LERIQKQITLLQERKQIIISEVVTGKIKVS

Sequences:

>Translated_430_residues
MEKYNSYKDSGIQWLGKIPSHWEIKRSRLIFDENVETNSTCNNTNQLQFRFGTIEPKKSQEMDSDLKKIISKYTIVQNGD
IMINGLNLNYDFVSQRVAQVKEKGIITSAYIALRPKENICSDYFTYLLKGMDARKVFHGMGCGVRLTLSFKEFRNELLPI
PPLEEQQSMATYLDKATAEIDKAIAQQQRMIDLLNERKQIIIQRAVTKGLDGNVEMKNSGLNWLGQIPSHWESLPLTYVF
EMRNGYTPSKNDPTYWTNGSIPWYRMEDIRKSGRFLREAMQYVTTKAINGKGTFKAGSYIMAICTASIGEHAMLIADSLA
NQRFANFKIRKSLIESFYPLFLFYYMYVVGDFCRENSNSTCFQYVDMGALKRFPIPKPSMEEQKNIVSSLTQNLQQINTA
LERIQKQITLLQERKQIIISEVVTGKIKVS
>Mature_430_residues
MEKYNSYKDSGIQWLGKIPSHWEIKRSRLIFDENVETNSTCNNTNQLQFRFGTIEPKKSQEMDSDLKKIISKYTIVQNGD
IMINGLNLNYDFVSQRVAQVKEKGIITSAYIALRPKENICSDYFTYLLKGMDARKVFHGMGCGVRLTLSFKEFRNELLPI
PPLEEQQSMATYLDKATAEIDKAIAQQQRMIDLLNERKQIIIQRAVTKGLDGNVEMKNSGLNWLGQIPSHWESLPLTYVF
EMRNGYTPSKNDPTYWTNGSIPWYRMEDIRKSGRFLREAMQYVTTKAINGKGTFKAGSYIMAICTASIGEHAMLIADSLA
NQRFANFKIRKSLIESFYPLFLFYYMYVVGDFCRENSNSTCFQYVDMGALKRFPIPKPSMEEQKNIVSSLTQNLQQINTA
LERIQKQITLLQERKQIIISEVVTGKIKVS

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 49551; Mature: 49551

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYNSYKDSGIQWLGKIPSHWEIKRSRLIFDENVETNSTCNNTNQLQFRFGTIEPKKSQ
CCCCCCCHHHHHHHHHCCCCCHHHHHHHEEEECCCCCCCCCCCCCEEEEEECCCCCCHHH
EMDSDLKKIISKYTIVQNGDIMINGLNLNYDFVSQRVAQVKEKGIITSAYIALRPKENIC
HHHHHHHHHHHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHH
SDYFTYLLKGMDARKVFHGMGCGVRLTLSFKEFRNELLPIPPLEEQQSMATYLDKATAEI
HHHHHHHHCCCCHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DKAIAQQQRMIDLLNERKQIIIQRAVTKGLDGNVEMKNSGLNWLGQIPSHWESLPLTYVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCHHHHCCCCEEEE
EMRNGYTPSKNDPTYWTNGSIPWYRMEDIRKSGRFLREAMQYVTTKAINGKGTFKAGSYI
EECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
MAICTASIGEHAMLIADSLANQRFANFKIRKSLIESFYPLFLFYYMYVVGDFCRENSNST
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
CFQYVDMGALKRFPIPKPSMEEQKNIVSSLTQNLQQINTALERIQKQITLLQERKQIIIS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVTGKIKVS
HHHCCCEEEC
>Mature Secondary Structure
MEKYNSYKDSGIQWLGKIPSHWEIKRSRLIFDENVETNSTCNNTNQLQFRFGTIEPKKSQ
CCCCCCCHHHHHHHHHCCCCCHHHHHHHEEEECCCCCCCCCCCCCEEEEEECCCCCCHHH
EMDSDLKKIISKYTIVQNGDIMINGLNLNYDFVSQRVAQVKEKGIITSAYIALRPKENIC
HHHHHHHHHHHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHH
SDYFTYLLKGMDARKVFHGMGCGVRLTLSFKEFRNELLPIPPLEEQQSMATYLDKATAEI
HHHHHHHHCCCCHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DKAIAQQQRMIDLLNERKQIIIQRAVTKGLDGNVEMKNSGLNWLGQIPSHWESLPLTYVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCHHHHCCCCEEEE
EMRNGYTPSKNDPTYWTNGSIPWYRMEDIRKSGRFLREAMQYVTTKAINGKGTFKAGSYI
EECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
MAICTASIGEHAMLIADSLANQRFANFKIRKSLIESFYPLFLFYYMYVVGDFCRENSNST
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
CFQYVDMGALKRFPIPKPSMEEQKNIVSSLTQNLQQINTALERIQKQITLLQERKQIIIS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVTGKIKVS
HHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1691706 [H]