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The map label for this gene is mrcA [H]

Identifier: 150005840

GI number: 150005840

Start: 4170003

End: 4172330

Strand: Reverse

Name: mrcA [H]

Synonym: BVU_3336

Alternate gene names: 150005840

Gene position: 4172330-4170003 (Counterclockwise)

Preceding gene: 150005849

Following gene: 150005839

Centisome position: 80.81

GC content: 43.13

Gene sequence:

>2328_bases
ATGAGGAAGAAATTTATATACGTGCTTTGGGCTCTTGTAGTCGGCGTACTGTTAGTTATAGCGCTTATATTCACCGCAAT
AGCGAAAGGATGGATTGGATATGTACCGCCTATTGAGGAATTGGAAAATCCGAATTTAAAGTTTGCAACCGAGATTATAT
CCGATGACGGCAAGATGCTGGGTACTTGGTCACTTAGTAAGGAGAATCGTGTTTTTGTAGGTTATGACCAGCTTTCATCG
AATCTGATTCGTGCGCTGATTGCTACCGAAGATGAACGTTTTGCAGAACATTCCGGCATTGATGCCCGTGCGTTTGTCCG
TGCTTTGGTGAAACGGGGAGTGATGATGCAGAAGAATGCCGGTGGGGGTAGTACCATAACCCAGCAGTTGGCAAAACAAT
TCTATTCACCTACGGCAGATAATGTGATGGAACGTCTGTTGCAGAAGCCCATTGAATGGGTAATAGCTGTTCAGTTGGAA
CGCTATTATACAAAGGAAGAGATTCTGACTATGTATCTTAATAAGTTCGATTTCTTGAATAATGCAGTAGGTATCAAAAC
AGCTGCCAATACTTACTTCTCGAAAGAACCGAAAGATTTGAGCATTGAGGAAGCGGCTACTTTGGTTGGTATGTGTAAGA
ATCCGTCTCTTTATAATCCGAAACGTTTTAATGAACGTTCACGTGGACGGCGTAATGTGGTGCTTGGCCAGATGCGGAAG
GCGGGATACCTGACTGATGCGGAAACTGATTCATTAAAGGCGTTGCCTCTCGTCTTAAAATATCGTCGTGTGGATCATAA
AGAAGGTTTGGCAACTTACTTCCGTGAATATCTGCGTGGAGTAATGACAGCCAAGGAGCCGAAGAAAAGTGATTATCGCG
GATGGCAGATGCAGAAATATTATGAAGATTCATTGGCATGGAAAACGAATCCGCTTTTCGGGTGGTGTGCTAAAAACAAG
AAGAAAGACGGTACCAACTACAATATTTATACAGACGGTCTTAAGATCTATACCACTATTGATTCACGTATGCAGAAATA
TGCGGAAGAGGCTGTTTATGAACATGTGGCGCAATACTTGCAACCTCGTTTCTTCAAGGAAAAACGGAAAAAGAAAACAG
CTCCGTTTACTAATCAGTTGACAGAGGAGGAGGTGAATACCATCATGACCAGGGCTATGAAACAGACTGATCGTTATCGT
ATTATGAAAGAAGCCGGATGCTCGGAAGCGGAAATAAAGAAAGCGTTTAATACTAAATACGAAATGTCTGTATTTTCTTA
CGAGGGAGAGAAGGATACCATTATGACTCCGATGGATTCTTTGAAATATTATAAATTCTTCCTTAGGGCGGGATTTATGT
CTATGGATCCCTTGACAGGTCATGTGAAGGCGTATGTGGGTGGTCCTAACTATAATTATTTCCAATATGATATGGCGATG
GTGGGGCGTCGCCAAGTAGGTTCTACTATTAAACCTTACGTGTATACTTTGGCTATGGAGAACGGTTTCTCTCCCTGTGA
TCAGGTGCGTCACGTAGAGCAGACATTGATAGATGAGAACGGGCGTCCATGGTCACCACGTAATGCCAGCAAAAAGCGTT
ATGGAGAAATGGTAACTATCAAATGGGGATTGGCTAATTCGGATAATTGGGTAACGGCTTATTTGATGGGGAAACTTAAT
CCTTATCAGTTGGTTCGTCTGATTCATTCATTCGGTGTGCAGAACAAACAGATAGACCCCGTAGTATCACTCTGTTTGGG
ACCTTGTGAAATTTCTGTAGGGGAGATGGTCAGCGCTTATTCAGCATTCGCAAACCGAGGTATCCGTACAGCACCAGTAT
TTGTAACCCGTATTGAGGATAATGAAGGTAATGTATTAGCGAATTTCACTCCGCAAATGGATGAGGTGATTAGTGAGACC
AGTGCTTACAAAATGCTGGTTATGTTGCGTGCGGTCGTTAATGAGGGTACCGGTGGACGTGTACGCCGTCTGTATGGCAT
TACTGCTGATATGGGGGGGAAGACAGGTACAACCAACCGTAACTCTGACGGTTGGTTTATGGGATTCACTCCCTCATTGG
TGTCCGGTGTATGGGTAGGCGGTGAAGAACGTGATATTCATTTTGATACGATGGTAGACGGGCAGGGGGCTGCTATGGCG
CTTCCTATTTGGGGACTTTATATGCAGAAAGTCTATAAAGACAAGAGCTTGGGATATTCTCAGGATGAGAAATTCAATAT
TCCTGATGACTTTGACCCTTGCAAAGATACCTTAATAGATGAAACTTCGGTAGAAGAAACCGGAGGTTTGGATAATATAT
TTGAATAA

Upstream 100 bases:

>100_bases
TGAGCGAAGAATTAAAAGATATTTCGTTGAACTTTGTTAGAAACACATTCTATCTCAAAGTTTTTCCTTACTTTTGTACC
GTTAATTACTAGAAAATGTC

Downstream 100 bases:

>100_bases
TTGAAAGATTTATCAATAAAAAAGTCTGTTTGCAAGGAAAAGCAAACAGACTTTTTTTATTGATATACCTTTTATAAAAG
GTGCTTTCTCTACTTGGTGT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MRKKFIYVLWALVVGVLLVIALIFTAIAKGWIGYVPPIEELENPNLKFATEIISDDGKMLGTWSLSKENRVFVGYDQLSS
NLIRALIATEDERFAEHSGIDARAFVRALVKRGVMMQKNAGGGSTITQQLAKQFYSPTADNVMERLLQKPIEWVIAVQLE
RYYTKEEILTMYLNKFDFLNNAVGIKTAANTYFSKEPKDLSIEEAATLVGMCKNPSLYNPKRFNERSRGRRNVVLGQMRK
AGYLTDAETDSLKALPLVLKYRRVDHKEGLATYFREYLRGVMTAKEPKKSDYRGWQMQKYYEDSLAWKTNPLFGWCAKNK
KKDGTNYNIYTDGLKIYTTIDSRMQKYAEEAVYEHVAQYLQPRFFKEKRKKKTAPFTNQLTEEEVNTIMTRAMKQTDRYR
IMKEAGCSEAEIKKAFNTKYEMSVFSYEGEKDTIMTPMDSLKYYKFFLRAGFMSMDPLTGHVKAYVGGPNYNYFQYDMAM
VGRRQVGSTIKPYVYTLAMENGFSPCDQVRHVEQTLIDENGRPWSPRNASKKRYGEMVTIKWGLANSDNWVTAYLMGKLN
PYQLVRLIHSFGVQNKQIDPVVSLCLGPCEISVGEMVSAYSAFANRGIRTAPVFVTRIEDNEGNVLANFTPQMDEVISET
SAYKMLVMLRAVVNEGTGGRVRRLYGITADMGGKTGTTNRNSDGWFMGFTPSLVSGVWVGGEERDIHFDTMVDGQGAAMA
LPIWGLYMQKVYKDKSLGYSQDEKFNIPDDFDPCKDTLIDETSVEETGGLDNIFE

Sequences:

>Translated_775_residues
MRKKFIYVLWALVVGVLLVIALIFTAIAKGWIGYVPPIEELENPNLKFATEIISDDGKMLGTWSLSKENRVFVGYDQLSS
NLIRALIATEDERFAEHSGIDARAFVRALVKRGVMMQKNAGGGSTITQQLAKQFYSPTADNVMERLLQKPIEWVIAVQLE
RYYTKEEILTMYLNKFDFLNNAVGIKTAANTYFSKEPKDLSIEEAATLVGMCKNPSLYNPKRFNERSRGRRNVVLGQMRK
AGYLTDAETDSLKALPLVLKYRRVDHKEGLATYFREYLRGVMTAKEPKKSDYRGWQMQKYYEDSLAWKTNPLFGWCAKNK
KKDGTNYNIYTDGLKIYTTIDSRMQKYAEEAVYEHVAQYLQPRFFKEKRKKKTAPFTNQLTEEEVNTIMTRAMKQTDRYR
IMKEAGCSEAEIKKAFNTKYEMSVFSYEGEKDTIMTPMDSLKYYKFFLRAGFMSMDPLTGHVKAYVGGPNYNYFQYDMAM
VGRRQVGSTIKPYVYTLAMENGFSPCDQVRHVEQTLIDENGRPWSPRNASKKRYGEMVTIKWGLANSDNWVTAYLMGKLN
PYQLVRLIHSFGVQNKQIDPVVSLCLGPCEISVGEMVSAYSAFANRGIRTAPVFVTRIEDNEGNVLANFTPQMDEVISET
SAYKMLVMLRAVVNEGTGGRVRRLYGITADMGGKTGTTNRNSDGWFMGFTPSLVSGVWVGGEERDIHFDTMVDGQGAAMA
LPIWGLYMQKVYKDKSLGYSQDEKFNIPDDFDPCKDTLIDETSVEETGGLDNIFE
>Mature_775_residues
MRKKFIYVLWALVVGVLLVIALIFTAIAKGWIGYVPPIEELENPNLKFATEIISDDGKMLGTWSLSKENRVFVGYDQLSS
NLIRALIATEDERFAEHSGIDARAFVRALVKRGVMMQKNAGGGSTITQQLAKQFYSPTADNVMERLLQKPIEWVIAVQLE
RYYTKEEILTMYLNKFDFLNNAVGIKTAANTYFSKEPKDLSIEEAATLVGMCKNPSLYNPKRFNERSRGRRNVVLGQMRK
AGYLTDAETDSLKALPLVLKYRRVDHKEGLATYFREYLRGVMTAKEPKKSDYRGWQMQKYYEDSLAWKTNPLFGWCAKNK
KKDGTNYNIYTDGLKIYTTIDSRMQKYAEEAVYEHVAQYLQPRFFKEKRKKKTAPFTNQLTEEEVNTIMTRAMKQTDRYR
IMKEAGCSEAEIKKAFNTKYEMSVFSYEGEKDTIMTPMDSLKYYKFFLRAGFMSMDPLTGHVKAYVGGPNYNYFQYDMAM
VGRRQVGSTIKPYVYTLAMENGFSPCDQVRHVEQTLIDENGRPWSPRNASKKRYGEMVTIKWGLANSDNWVTAYLMGKLN
PYQLVRLIHSFGVQNKQIDPVVSLCLGPCEISVGEMVSAYSAFANRGIRTAPVFVTRIEDNEGNVLANFTPQMDEVISET
SAYKMLVMLRAVVNEGTGGRVRRLYGITADMGGKTGTTNRNSDGWFMGFTPSLVSGVWVGGEERDIHFDTMVDGQGAAMA
LPIWGLYMQKVYKDKSLGYSQDEKFNIPDDFDPCKDTLIDETSVEETGGLDNIFE

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=666, Percent_Identity=27.6276276276276, Blast_Score=213, Evalue=5e-56,
Organism=Escherichia coli, GI1786343, Length=279, Percent_Identity=31.5412186379928, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788867, Length=247, Percent_Identity=30.3643724696356, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1789601, Length=174, Percent_Identity=31.6091954022989, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 88341; Mature: 88341

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKFIYVLWALVVGVLLVIALIFTAIAKGWIGYVPPIEELENPNLKFATEIISDDGKML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEHHHHHCCCCCEE
GTWSLSKENRVFVGYDQLSSNLIRALIATEDERFAEHSGIDARAFVRALVKRGVMMQKNA
EEEECCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCEEECCC
GGGSTITQQLAKQFYSPTADNVMERLLQKPIEWVIAVQLERYYTKEEILTMYLNKFDFLN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NAVGIKTAANTYFSKEPKDLSIEEAATLVGMCKNPSLYNPKRFNERSRGRRNVVLGQMRK
CCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCHHHCCCHHHHHHHHHH
AGYLTDAETDSLKALPLVLKYRRVDHKEGLATYFREYLRGVMTAKEPKKSDYRGWQMQKY
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
YEDSLAWKTNPLFGWCAKNKKKDGTNYNIYTDGLKIYTTIDSRMQKYAEEAVYEHVAQYL
HHHHCCEECCCCEEEECCCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHH
QPRFFKEKRKKKTAPFTNQLTEEEVNTIMTRAMKQTDRYRIMKEAGCSEAEIKKAFNTKY
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCE
EMSVFSYEGEKDTIMTPMDSLKYYKFFLRAGFMSMDPLTGHVKAYVGGPNYNYFQYDMAM
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEHHHHH
VGRRQVGSTIKPYVYTLAMENGFSPCDQVRHVEQTLIDENGRPWSPRNASKKRYGEMVTI
HHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEE
KWGLANSDNWVTAYLMGKLNPYQLVRLIHSFGVQNKQIDPVVSLCLGPCEISVGEMVSAY
EEECCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHH
SAFANRGIRTAPVFVTRIEDNEGNVLANFTPQMDEVISETSAYKMLVMLRAVVNEGTGGR
HHHHHCCCEECCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VRRLYGITADMGGKTGTTNRNSDGWFMGFTPSLVSGVWVGGEERDIHFDTMVDGQGAAMA
EEEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHCCEECCCCCCCEEEEEEECCCCCEEE
LPIWGLYMQKVYKDKSLGYSQDEKFNIPDDFDPCKDTLIDETSVEETGGLDNIFE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHCCCCHHCCC
>Mature Secondary Structure
MRKKFIYVLWALVVGVLLVIALIFTAIAKGWIGYVPPIEELENPNLKFATEIISDDGKML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEHHHHHCCCCCEE
GTWSLSKENRVFVGYDQLSSNLIRALIATEDERFAEHSGIDARAFVRALVKRGVMMQKNA
EEEECCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCEEECCC
GGGSTITQQLAKQFYSPTADNVMERLLQKPIEWVIAVQLERYYTKEEILTMYLNKFDFLN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NAVGIKTAANTYFSKEPKDLSIEEAATLVGMCKNPSLYNPKRFNERSRGRRNVVLGQMRK
CCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCHHHCCCHHHHHHHHHH
AGYLTDAETDSLKALPLVLKYRRVDHKEGLATYFREYLRGVMTAKEPKKSDYRGWQMQKY
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
YEDSLAWKTNPLFGWCAKNKKKDGTNYNIYTDGLKIYTTIDSRMQKYAEEAVYEHVAQYL
HHHHCCEECCCCEEEECCCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHH
QPRFFKEKRKKKTAPFTNQLTEEEVNTIMTRAMKQTDRYRIMKEAGCSEAEIKKAFNTKY
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCE
EMSVFSYEGEKDTIMTPMDSLKYYKFFLRAGFMSMDPLTGHVKAYVGGPNYNYFQYDMAM
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEHHHHH
VGRRQVGSTIKPYVYTLAMENGFSPCDQVRHVEQTLIDENGRPWSPRNASKKRYGEMVTI
HHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEE
KWGLANSDNWVTAYLMGKLNPYQLVRLIHSFGVQNKQIDPVVSLCLGPCEISVGEMVSAY
EEECCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHH
SAFANRGIRTAPVFVTRIEDNEGNVLANFTPQMDEVISETSAYKMLVMLRAVVNEGTGGR
HHHHHCCCEECCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VRRLYGITADMGGKTGTTNRNSDGWFMGFTPSLVSGVWVGGEERDIHFDTMVDGQGAAMA
EEEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHCCEECCCCCCCEEEEEEECCCCCEEE
LPIWGLYMQKVYKDKSLGYSQDEKFNIPDDFDPCKDTLIDETSVEETGGLDNIFE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]