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The map label for this gene is 150005730

Identifier: 150005730

GI number: 150005730

Start: 4047045

End: 4047932

Strand: Reverse

Name: 150005730

Synonym: BVU_3223

Alternate gene names: NA

Gene position: 4047932-4047045 (Counterclockwise)

Preceding gene: 150005731

Following gene: 150005729

Centisome position: 78.4

GC content: 47.07

Gene sequence:

>888_bases
ATGAAAAGAACGATTAAAGTAGGAATTATCCAACAGACATGCACTAATGATATCCGTCACAATTTGAGCAAACTGCACCA
CAATATAGAACAGGTGGCGGCAGCAGGTGCACAGCTGGTTGTATTGCAGGAGTTGCACAACACCCCTTACTTCTGTCAGA
CAGAAGATACGGATATGTTTGATTTGGCAGAACCGGTTCCCGGTCCTTCTACCGGCTTCTACAGTGAGTTGGCTGCCGGA
TACGGTATTGTACTGGTTACTTCGTTATTCGAGAAGCGTGCTCCGGGGCTATATCACAATACAGCCGTCGTTTTTGACAA
AGATGGCAGTATAGCAGGGAAATACCGCAAAATGCATATTCCCGATGATCCCGCCTATTATGAGAAATTCTATTTTACTC
CCGGTGACCTGGGATTTGAACCTATTCAGACATCACTGGGAAAACTAGGTGTACAAGTCTGCTGGGACCAATGGTATCCC
GAAGGTGCACGGCTGATGGCACTGAAAGGTGCTGAAATTCTTATATACCCTACAGCTATAGGTTGGGAAAGCACAGACAC
TCAAGAAGAGAAAATCCGTCAACTAGGTGCATGGGTAACCGTGCAGCGTGGACATGCCGTGGCAAACGGTCTGCCGGTTA
TCGCCGTGAACCGTGTAGGACATGAGCCCGATCCTTCCGGTCAGACTAATGGTATCCAATTTTGGGGTAATAGCTTCGTG
GCAGGTCCTCAAGGAGAAATACTGGCACAAGCCAACAATATGGATGAAGAAAATATGGTAGTGGAACTGGATATGACCCG
TAGTGAGAATGTTCGTCGCTGGTGGCCTTTCCTCCGTGACCGGCGTATTGATGAGTTTGAAAATCTAACCCGACGCTTTA
TAGATTAA

Upstream 100 bases:

>100_bases
AGATAGTAGGTATTGACTGCACTGCTTTAATAAAGCAACATGGCTCGCTGCATTGCGTCACCATGCAATATCCCGAAGGT
GTATATTAATAAATATATGT

Downstream 100 bases:

>100_bases
ATACAAACGTTATCGTTATATAAATTCACATATTCGTTAAACAATTCATGGTTAAGGCTGTTTTAGTTATCAGAATATTC
AAAAGATATTATTGATTTAC

Product: beta-ureidopropionase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKRTIKVGIIQQTCTNDIRHNLSKLHHNIEQVAAAGAQLVVLQELHNTPYFCQTEDTDMFDLAEPVPGPSTGFYSELAAG
YGIVLVTSLFEKRAPGLYHNTAVVFDKDGSIAGKYRKMHIPDDPAYYEKFYFTPGDLGFEPIQTSLGKLGVQVCWDQWYP
EGARLMALKGAEILIYPTAIGWESTDTQEEKIRQLGAWVTVQRGHAVANGLPVIAVNRVGHEPDPSGQTNGIQFWGNSFV
AGPQGEILAQANNMDEENMVVELDMTRSENVRRWWPFLRDRRIDEFENLTRRFID

Sequences:

>Translated_295_residues
MKRTIKVGIIQQTCTNDIRHNLSKLHHNIEQVAAAGAQLVVLQELHNTPYFCQTEDTDMFDLAEPVPGPSTGFYSELAAG
YGIVLVTSLFEKRAPGLYHNTAVVFDKDGSIAGKYRKMHIPDDPAYYEKFYFTPGDLGFEPIQTSLGKLGVQVCWDQWYP
EGARLMALKGAEILIYPTAIGWESTDTQEEKIRQLGAWVTVQRGHAVANGLPVIAVNRVGHEPDPSGQTNGIQFWGNSFV
AGPQGEILAQANNMDEENMVVELDMTRSENVRRWWPFLRDRRIDEFENLTRRFID
>Mature_295_residues
MKRTIKVGIIQQTCTNDIRHNLSKLHHNIEQVAAAGAQLVVLQELHNTPYFCQTEDTDMFDLAEPVPGPSTGFYSELAAG
YGIVLVTSLFEKRAPGLYHNTAVVFDKDGSIAGKYRKMHIPDDPAYYEKFYFTPGDLGFEPIQTSLGKLGVQVCWDQWYP
EGARLMALKGAEILIYPTAIGWESTDTQEEKIRQLGAWVTVQRGHAVANGLPVIAVNRVGHEPDPSGQTNGIQFWGNSFV
AGPQGEILAQANNMDEENMVVELDMTRSENVRRWWPFLRDRRIDEFENLTRRFID

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=297, Percent_Identity=28.956228956229, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI9910460, Length=288, Percent_Identity=27.4305555555556, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI297632348, Length=295, Percent_Identity=27.4576271186441, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632350, Length=295, Percent_Identity=27.4576271186441, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI5031947, Length=295, Percent_Identity=27.4576271186441, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI297632346, Length=185, Percent_Identity=30.2702702702703, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17533173, Length=305, Percent_Identity=29.1803278688525, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17556280, Length=289, Percent_Identity=25.9515570934256, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323383, Length=304, Percent_Identity=30.2631578947368, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322335, Length=311, Percent_Identity=24.4372990353698, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358471, Length=307, Percent_Identity=30.9446254071661, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17933642, Length=285, Percent_Identity=25.6140350877193, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21355835, Length=292, Percent_Identity=25.6849315068493, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33259; Mature: 33259

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTIKVGIIQQTCTNDIRHNLSKLHHNIEQVAAAGAQLVVLQELHNTPYFCQTEDTDMF
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCEEECCCCCHH
DLAEPVPGPSTGFYSELAAGYGIVLVTSLFEKRAPGLYHNTAVVFDKDGSIAGKYRKMHI
HHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEECEEEEEECCCCCCCCEEEEEC
PDDPAYYEKFYFTPGDLGFEPIQTSLGKLGVQVCWDQWYPEGARLMALKGAEILIYPTAI
CCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEC
GWESTDTQEEKIRQLGAWVTVQRGHAVANGLPVIAVNRVGHEPDPSGQTNGIQFWGNSFV
CCCCCCCHHHHHHHHCCEEEEECCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEECCEEC
AGPQGEILAQANNMDEENMVVELDMTRSENVRRWWPFLRDRRIDEFENLTRRFID
CCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKRTIKVGIIQQTCTNDIRHNLSKLHHNIEQVAAAGAQLVVLQELHNTPYFCQTEDTDMF
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCEEECCCCCHH
DLAEPVPGPSTGFYSELAAGYGIVLVTSLFEKRAPGLYHNTAVVFDKDGSIAGKYRKMHI
HHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEECEEEEEECCCCCCCCEEEEEC
PDDPAYYEKFYFTPGDLGFEPIQTSLGKLGVQVCWDQWYPEGARLMALKGAEILIYPTAI
CCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEC
GWESTDTQEEKIRQLGAWVTVQRGHAVANGLPVIAVNRVGHEPDPSGQTNGIQFWGNSFV
CCCCCCCHHHHHHHHCCEEEEECCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEECCEEC
AGPQGEILAQANNMDEENMVVELDMTRSENVRRWWPFLRDRRIDEFENLTRRFID
CCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]