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The map label for this gene is tgt [H]

Identifier: 150005672

GI number: 150005672

Start: 3980059

End: 3981189

Strand: Reverse

Name: tgt [H]

Synonym: BVU_3162

Alternate gene names: 150005672

Gene position: 3981189-3980059 (Counterclockwise)

Preceding gene: 150005673

Following gene: 150005671

Centisome position: 77.11

GC content: 45.8

Gene sequence:

>1131_bases
ATGACTTTTGACTTACAATATACAGACCCCAAATCGAATGCCCGTGCCGGATTGATAACAACTGACCACGGGCAAATAGA
AACCCCCATCTTTATGCCTGTAGGCACATTGGGAACGGTGAAGGGCGTGCATTTACATGAGTTGAAAGAGGACATTAAAG
CACAGATAATCCTGGGCAACACTTACCACCTGTATTTACGACCGGGACTGGATATAATAGAACGTGCCGGAGGTCTGCAT
AAATTCAACGGCTTTGACCGTCCTATGCTGACTGACAGTGGTGGTTTTCAGGTATTCTCCTTATCAGGTATCCGCAAAAT
GAGAGAAGAAGGTGTGGAATTTCGCTCACATATAGACGGTTCGAAACACCTGTTCACTCCTGAACGGGTGATGGACATAG
AACGGACTATCGGTGCAGATATCATGATGGCTTTTGACGAATGTACTCCGGGAACCGCAGATTATGAATACGCAAAAAAA
TCAATGCAGCTTACCCATCGTTGGCTGGACCGTTGCATAAAGCATTTCAACGAAACCGATCCCAAATATGGTTATCAACA
GTCATTATTTCCCATAGTACAAGGCTGTGTCTATCCCGACTTACGCAAACAATCAGCGGAATATATTGCCTCCAAGGGAG
CTGACGGAAATGCCATCGGTGGCCTGGCCGTAGGTGAGCCAACCGAGAAAATGTACGAAATGATAGAGGTTGTCAATGAA
ATCTTACCCAAAGACAAGCCCCGCTATCTGATGGGAGTCGGTACACCGGTAAATATACTGGAGGGTATAGAACGCGGTGT
AGATATGTTTGATTGTGTAATGCCGACCCGGAACGGACGCAATGGAATGTTATTCACTAAAAACGGCATAATGAACATGC
GCAACAAGAAATGGGAGGATGATTATTCTCCTATCGAGGCCGAAGGAGCATCGTATGTAGATACCATGTACAGCCGTGCT
TACTTGCGCCATCTGTTCCACGCACAAGAACTGCTGGCCATGCAAATCGCTTCTATCCACAACTTGGCATTCTATCTGTG
GCTAGCAGGCGAGGCCCGTAAACACATCATTGCAGGAGATTTCTCTACGTGGAAACCAATGATGGTAAAACGCTTATCTA
CAAGATTATAA

Upstream 100 bases:

>100_bases
ATATTAAGGAAGTATTGGCTTTGGCTCTCACCAATGAAAAAGTAAACGATGCCATTGATTTTAATATTACCAATCAAAAA
ACAGAAGAAAAGAAATAAAT

Downstream 100 bases:

>100_bases
ATTACTAGAAATGAAGAATTGGTTCAAGACAAACACAGATTCATCCCCAAAGGATAAAAAATGGTGGAAGCCGAATATCA
GTTGGCTGAAACTGGATAAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTFDLQYTDPKSNARAGLITTDHGQIETPIFMPVGTLGTVKGVHLHELKEDIKAQIILGNTYHLYLRPGLDIIERAGGLH
KFNGFDRPMLTDSGGFQVFSLSGIRKMREEGVEFRSHIDGSKHLFTPERVMDIERTIGADIMMAFDECTPGTADYEYAKK
SMQLTHRWLDRCIKHFNETDPKYGYQQSLFPIVQGCVYPDLRKQSAEYIASKGADGNAIGGLAVGEPTEKMYEMIEVVNE
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKNGIMNMRNKKWEDDYSPIEAEGASYVDTMYSRA
YLRHLFHAQELLAMQIASIHNLAFYLWLAGEARKHIIAGDFSTWKPMMVKRLSTRL

Sequences:

>Translated_376_residues
MTFDLQYTDPKSNARAGLITTDHGQIETPIFMPVGTLGTVKGVHLHELKEDIKAQIILGNTYHLYLRPGLDIIERAGGLH
KFNGFDRPMLTDSGGFQVFSLSGIRKMREEGVEFRSHIDGSKHLFTPERVMDIERTIGADIMMAFDECTPGTADYEYAKK
SMQLTHRWLDRCIKHFNETDPKYGYQQSLFPIVQGCVYPDLRKQSAEYIASKGADGNAIGGLAVGEPTEKMYEMIEVVNE
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKNGIMNMRNKKWEDDYSPIEAEGASYVDTMYSRA
YLRHLFHAQELLAMQIASIHNLAFYLWLAGEARKHIIAGDFSTWKPMMVKRLSTRL
>Mature_375_residues
TFDLQYTDPKSNARAGLITTDHGQIETPIFMPVGTLGTVKGVHLHELKEDIKAQIILGNTYHLYLRPGLDIIERAGGLHK
FNGFDRPMLTDSGGFQVFSLSGIRKMREEGVEFRSHIDGSKHLFTPERVMDIERTIGADIMMAFDECTPGTADYEYAKKS
MQLTHRWLDRCIKHFNETDPKYGYQQSLFPIVQGCVYPDLRKQSAEYIASKGADGNAIGGLAVGEPTEKMYEMIEVVNEI
LPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKNGIMNMRNKKWEDDYSPIEAEGASYVDTMYSRAY
LRHLFHAQELLAMQIASIHNLAFYLWLAGEARKHIIAGDFSTWKPMMVKRLSTRL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=351, Percent_Identity=40.4558404558405, Blast_Score=281, Evalue=9e-76,
Organism=Escherichia coli, GI1786607, Length=364, Percent_Identity=49.4505494505495, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17544680, Length=344, Percent_Identity=38.3720930232558, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI25146502, Length=126, Percent_Identity=30.1587301587302, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19920510, Length=353, Percent_Identity=42.4929178470255, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42664; Mature: 42533

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDLQYTDPKSNARAGLITTDHGQIETPIFMPVGTLGTVKGVHLHELKEDIKAQIILGN
CEEEEEECCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECC
TYHLYLRPGLDIIERAGGLHKFNGFDRPMLTDSGGFQVFSLSGIRKMREEGVEFRSHIDG
EEEEEECCCHHHHHHCCCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHCCC
SKHLFTPERVMDIERTIGADIMMAFDECTPGTADYEYAKKSMQLTHRWLDRCIKHFNETD
CCCCCCHHHHHHHHHHHCHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PKYGYQQSLFPIVQGCVYPDLRKQSAEYIASKGADGNAIGGLAVGEPTEKMYEMIEVVNE
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKNGIMNMRNKKWED
HCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
DYSPIEAEGASYVDTMYSRAYLRHLFHAQELLAMQIASIHNLAFYLWLAGEARKHIIAGD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEECC
FSTWKPMMVKRLSTRL
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TFDLQYTDPKSNARAGLITTDHGQIETPIFMPVGTLGTVKGVHLHELKEDIKAQIILGN
EEEEEECCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECC
TYHLYLRPGLDIIERAGGLHKFNGFDRPMLTDSGGFQVFSLSGIRKMREEGVEFRSHIDG
EEEEEECCCHHHHHHCCCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHCCC
SKHLFTPERVMDIERTIGADIMMAFDECTPGTADYEYAKKSMQLTHRWLDRCIKHFNETD
CCCCCCHHHHHHHHHHHCHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PKYGYQQSLFPIVQGCVYPDLRKQSAEYIASKGADGNAIGGLAVGEPTEKMYEMIEVVNE
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKNGIMNMRNKKWED
HCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
DYSPIEAEGASYVDTMYSRAYLRHLFHAQELLAMQIASIHNLAFYLWLAGEARKHIIAGD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEECC
FSTWKPMMVKRLSTRL
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]