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The map label for this gene is ansB [H]

Identifier: 150005316

GI number: 150005316

Start: 3562012

End: 3563070

Strand: Direct

Name: ansB [H]

Synonym: BVU_2792

Alternate gene names: 150005316

Gene position: 3562012-3563070 (Clockwise)

Preceding gene: 150005315

Following gene: 150005317

Centisome position: 68.99

GC content: 46.46

Gene sequence:

>1059_bases
ATGAACGGATTAAGAAAACTCAGCCTTATGGCGGCAATGCTGGTATGTACCACATTGGCTGCTGTCTCCCAAAAGCCTAA
TATCCATATATTGGCTACAGGGGGGACTATTGCCGGAACAGGCGCTTCGGCTACTAAAACAAATTATACTGCCGGACAGG
TGGCTATCAGCACATTGCTGGAAGCGGTTCCCGAAGTGAACAAAATAGCCAATGTGACCGGGGAACAGATTGTAAAAATC
GGTTCTCAGGACATGAATGATGCCGTATGGCTGACGTTGGCCAAGCGTATCAACGAGCTTTTCAGCCGTGGAGATGTGGA
CGGTATCGTGATTACTCACGGAACTGACACAATGGAAGAAACCGCCTTTTTCCTGAACTTGACTGTGAAGAGTGACAAGC
CTGTTGTATTGGTAGGAGCCATGCGCCCCTCTACGGCCATGAGTGCGGACGGACCGTTGAACTTGTATAATGCGGTAGTT
ACTGCCGCTGCTAGGGAATCACGGGGTAAAGGTGTCGTAATTGCCATGAACGGTTTGATTTTGGGAGCCCACGGAGCCAT
GAAGACCAATACGGTGGATGTACAGACTTTCCAGTCTCCTAACTCCGGTGCGTTGGGATATGTATTGAATGGTAAGGTTT
TTTATAATATGGAATCTTTGAAACGCCATACCACCGGTTCCGATTTTGATGTGGCCCACCTTGACAAGCTGCCGAAAGTA
GGTATCGTTTACAGTTATTCCAATGTAGAGGCTGATGTTATGATCCCTTTTCTGAATAATGGTTACCAAGGTATTATTCA
TGCCGGTGTGGGGAATGGCAACATTCATCAGAATTTGTTTCCGATATTGGAAAAGGCCCGTCAGCAAGGTATTTTGGTAG
TGCGTTCCAGCCGTGTTCCTACCGGACCGACCACATTGGATGCCGAGGTGGATGATAATAAATATCAATTCGTGGCTTCA
CAAGAGTTGAATCCTCAGAAAGCGCGTGTGTTGTTGATGTTGGCATTAACTAAAACAAAAGACTGGAAGAAGATTCAGGA
ATACTTCAACGTATACTAG

Upstream 100 bases:

>100_bases
ATTCGATGTATACATATATATCATCGGCACATCTTCTTCTTTGTGTGAACAAAATTTCCAAATAATTATTATAGTAATAA
AACCTAAACTTGAAAAGATT

Downstream 100 bases:

>100_bases
TCCATCCGGCACTTTTTCTTTTTATCTCTTTTTCTTTTGCGCGCATAAAAGAAAAAGAGAATACATTATAACCTAAAACT
TTGACTTATGAAAAGAATAA

Product: L-asparaginase II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNGLRKLSLMAAMLVCTTLAAVSQKPNIHILATGGTIAGTGASATKTNYTAGQVAISTLLEAVPEVNKIANVTGEQIVKI
GSQDMNDAVWLTLAKRINELFSRGDVDGIVITHGTDTMEETAFFLNLTVKSDKPVVLVGAMRPSTAMSADGPLNLYNAVV
TAAARESRGKGVVIAMNGLILGAHGAMKTNTVDVQTFQSPNSGALGYVLNGKVFYNMESLKRHTTGSDFDVAHLDKLPKV
GIVYSYSNVEADVMIPFLNNGYQGIIHAGVGNGNIHQNLFPILEKARQQGILVVRSSRVPTGPTTLDAEVDDNKYQFVAS
QELNPQKARVLLMLALTKTKDWKKIQEYFNVY

Sequences:

>Translated_352_residues
MNGLRKLSLMAAMLVCTTLAAVSQKPNIHILATGGTIAGTGASATKTNYTAGQVAISTLLEAVPEVNKIANVTGEQIVKI
GSQDMNDAVWLTLAKRINELFSRGDVDGIVITHGTDTMEETAFFLNLTVKSDKPVVLVGAMRPSTAMSADGPLNLYNAVV
TAAARESRGKGVVIAMNGLILGAHGAMKTNTVDVQTFQSPNSGALGYVLNGKVFYNMESLKRHTTGSDFDVAHLDKLPKV
GIVYSYSNVEADVMIPFLNNGYQGIIHAGVGNGNIHQNLFPILEKARQQGILVVRSSRVPTGPTTLDAEVDDNKYQFVAS
QELNPQKARVLLMLALTKTKDWKKIQEYFNVY
>Mature_352_residues
MNGLRKLSLMAAMLVCTTLAAVSQKPNIHILATGGTIAGTGASATKTNYTAGQVAISTLLEAVPEVNKIANVTGEQIVKI
GSQDMNDAVWLTLAKRINELFSRGDVDGIVITHGTDTMEETAFFLNLTVKSDKPVVLVGAMRPSTAMSADGPLNLYNAVV
TAAARESRGKGVVIAMNGLILGAHGAMKTNTVDVQTFQSPNSGALGYVLNGKVFYNMESLKRHTTGSDFDVAHLDKLPKV
GIVYSYSNVEADVMIPFLNNGYQGIIHAGVGNGNIHQNLFPILEKARQQGILVVRSSRVPTGPTTLDAEVDDNKYQFVAS
QELNPQKARVLLMLALTKTKDWKKIQEYFNVY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=229, Percent_Identity=28.82096069869, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789327, Length=347, Percent_Identity=59.9423631123919, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1788065, Length=285, Percent_Identity=27.719298245614, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17558280, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=355, Percent_Identity=37.1830985915493, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323187, Length=355, Percent_Identity=37.1830985915493, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323186, Length=355, Percent_Identity=37.1830985915493, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323184, Length=355, Percent_Identity=37.1830985915493, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6320527, Length=333, Percent_Identity=34.2342342342342, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24639609, Length=229, Percent_Identity=27.0742358078603, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37840; Mature: 37840

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLRKLSLMAAMLVCTTLAAVSQKPNIHILATGGTIAGTGASATKTNYTAGQVAISTLL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHH
EAVPEVNKIANVTGEQIVKIGSQDMNDAVWLTLAKRINELFSRGDVDGIVITHGTDTMEE
HHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHC
TAFFLNLTVKSDKPVVLVGAMRPSTAMSADGPLNLYNAVVTAAARESRGKGVVIAMNGLI
EEEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEE
LGAHGAMKTNTVDVQTFQSPNSGALGYVLNGKVFYNMESLKRHTTGSDFDVAHLDKLPKV
EECCCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEHHHHHHCCCCCCCCHHHHHCCCCC
GIVYSYSNVEADVMIPFLNNGYQGIIHAGVGNGNIHQNLFPILEKARQQGILVVRSSRVP
EEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCC
TGPTTLDAEVDDNKYQFVASQELNPQKARVLLMLALTKTKDWKKIQEYFNVY
CCCCEEEEEECCCEEEEEECCCCCHHHHEEEEEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNGLRKLSLMAAMLVCTTLAAVSQKPNIHILATGGTIAGTGASATKTNYTAGQVAISTLL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHH
EAVPEVNKIANVTGEQIVKIGSQDMNDAVWLTLAKRINELFSRGDVDGIVITHGTDTMEE
HHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHC
TAFFLNLTVKSDKPVVLVGAMRPSTAMSADGPLNLYNAVVTAAARESRGKGVVIAMNGLI
EEEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEE
LGAHGAMKTNTVDVQTFQSPNSGALGYVLNGKVFYNMESLKRHTTGSDFDVAHLDKLPKV
EECCCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEHHHHHHCCCCCCCCHHHHHCCCCC
GIVYSYSNVEADVMIPFLNNGYQGIIHAGVGNGNIHQNLFPILEKARQQGILVVRSSRVP
EEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCC
TGPTTLDAEVDDNKYQFVASQELNPQKARVLLMLALTKTKDWKKIQEYFNVY
CCCCEEEEEECCCEEEEEECCCCCHHHHEEEEEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]