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The map label for this gene is panB

Identifier: 150005274

GI number: 150005274

Start: 3503073

End: 3503894

Strand: Direct

Name: panB

Synonym: BVU_2746

Alternate gene names: 150005274

Gene position: 3503073-3503894 (Clockwise)

Preceding gene: 150005273

Following gene: 150005275

Centisome position: 67.85

GC content: 49.27

Gene sequence:

>822_bases
ATGGCAGGATATATTTCAGGTGATACACGCAAAGTAACTACCCACCGTTTGGTGGAAATGAAGCAGAGAGGCGAGAAAAT
TTCTATGCTTACCTCCTATGATTACACGACAGCGCAGATTGTAGATGGCGCAGGCATCGATGTTATATTGGTAGGCGACT
CCGCTTCGAATGTCATGGCAGGAAATGTGACCACACTTCCTATCACCCTTGATCAGATGATTTATCATGGAAAGTCTGTG
GTCCGTGGGGTGAAACGCGCGCTTGTTGTGGTGGATCTGCCTTTTGGCACTTACCAGACTTCCGAATACGAAGCGGTGAC
CAATGCGATCAAAGTAATGAAGATTACGCACGCCGATGCTTTGAAACTGGAAGGGGGCGTGGAGATCATTGATGCGGTAA
AGAAAATCATTGCTGCCGGCATTCCCATCATGGGACATTTGGGGTTAATGCCGCAATCTATCAATAAATATGGAACCTAT
ACTGTACGCGCCAAGGACGAGGAAGAGGCCGAGAAGTTGTTGAGCGATGCACATCTGCTGGAAGAGACAGGGTGTTTTGC
GCTGGTACTTGAGAAAATCCCTGCGTCACTGGCAGAGCGTGTGGCCAGGGAACTGACTATTCCCGTTATCGGTATCGGAG
CCGGTGGCGCGGTAGACGGGCAGGTGCTGGTAGTATCGGACATGCTGGGGATGACCAACGGTTTCAGTCCTCGTTTCCTC
CGTCGTTATGCCGATCTGCATACAGTTATGACGGGAGCCATACAGCAGTATGTGGATGATGTGAAAAAAGGGGATTTCCC
TAACGAAGACGAACAATATTAA

Upstream 100 bases:

>100_bases
GACTTTAATGGATTCAGTTTCGAAAAAATGAAGAATATGATGAGGTAATGTCAACTAAAACATCTACCTTTGCAGCCGTT
TTTTATAAATTAAAGTAAAT

Downstream 100 bases:

>100_bases
AAGTTTTTGCTGATATGTATGATGACTTCTTCTTTCCGTATGAAAAGGCAAAACTGTGGACTGGGAATATGTTTTTGTTA
TCCCTTTCCAATTTTTTGCT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MAGYISGDTRKVTTHRLVEMKQRGEKISMLTSYDYTTAQIVDGAGIDVILVGDSASNVMAGNVTTLPITLDQMIYHGKSV
VRGVKRALVVVDLPFGTYQTSEYEAVTNAIKVMKITHADALKLEGGVEIIDAVKKIIAAGIPIMGHLGLMPQSINKYGTY
TVRAKDEEEAEKLLSDAHLLEETGCFALVLEKIPASLAERVARELTIPVIGIGAGGAVDGQVLVVSDMLGMTNGFSPRFL
RRYADLHTVMTGAIQQYVDDVKKGDFPNEDEQY

Sequences:

>Translated_273_residues
MAGYISGDTRKVTTHRLVEMKQRGEKISMLTSYDYTTAQIVDGAGIDVILVGDSASNVMAGNVTTLPITLDQMIYHGKSV
VRGVKRALVVVDLPFGTYQTSEYEAVTNAIKVMKITHADALKLEGGVEIIDAVKKIIAAGIPIMGHLGLMPQSINKYGTY
TVRAKDEEEAEKLLSDAHLLEETGCFALVLEKIPASLAERVARELTIPVIGIGAGGAVDGQVLVVSDMLGMTNGFSPRFL
RRYADLHTVMTGAIQQYVDDVKKGDFPNEDEQY
>Mature_272_residues
AGYISGDTRKVTTHRLVEMKQRGEKISMLTSYDYTTAQIVDGAGIDVILVGDSASNVMAGNVTTLPITLDQMIYHGKSVV
RGVKRALVVVDLPFGTYQTSEYEAVTNAIKVMKITHADALKLEGGVEIIDAVKKIIAAGIPIMGHLGLMPQSINKYGTYT
VRAKDEEEAEKLLSDAHLLEETGCFALVLEKIPASLAERVARELTIPVIGIGAGGAVDGQVLVVSDMLGMTNGFSPRFLR
RYADLHTVMTGAIQQYVDDVKKGDFPNEDEQY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=39.0151515151515, Blast_Score=203, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6319653, Length=251, Percent_Identity=34.6613545816733, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BACV8 (A6L3Y2)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300018.1
- ProteinModelPortal:   A6L3Y2
- SMR:   A6L3Y2
- STRING:   A6L3Y2
- GeneID:   5303709
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2746
- NMPDR:   fig|435590.6.peg.2582
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29580; Mature: 29448

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: ACT_SITE 191-191 BINDING 92-92 BINDING 122-122

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGYISGDTRKVTTHRLVEMKQRGEKISMLTSYDYTTAQIVDGAGIDVILVGDSASNVMA
CCCCCCCCCEEHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCEEE
GNVTTLPITLDQMIYHGKSVVRGVKRALVVVDLPFGTYQTSEYEAVTNAIKVMKITHADA
CCEEEEEEEHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHEEECCCC
LKLEGGVEIIDAVKKIIAAGIPIMGHLGLMPQSINKYGTYTVRAKDEEEAEKLLSDAHLL
EEECCCHHHHHHHHHHHHCCCCHHHHCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHH
EETGCFALVLEKIPASLAERVARELTIPVIGIGAGGAVDGQVLVVSDMLGMTNGFSPRFL
HHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEHHHHCCCCCCHHHH
RRYADLHTVMTGAIQQYVDDVKKGDFPNEDEQY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AGYISGDTRKVTTHRLVEMKQRGEKISMLTSYDYTTAQIVDGAGIDVILVGDSASNVMA
CCCCCCCCEEHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCEEE
GNVTTLPITLDQMIYHGKSVVRGVKRALVVVDLPFGTYQTSEYEAVTNAIKVMKITHADA
CCEEEEEEEHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHEEECCCC
LKLEGGVEIIDAVKKIIAAGIPIMGHLGLMPQSINKYGTYTVRAKDEEEAEKLLSDAHLL
EEECCCHHHHHHHHHHHHCCCCHHHHCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHH
EETGCFALVLEKIPASLAERVARELTIPVIGIGAGGAVDGQVLVVSDMLGMTNGFSPRFL
HHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEHHHHCCCCCCHHHH
RRYADLHTVMTGAIQQYVDDVKKGDFPNEDEQY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA