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The map label for this gene is apbE [H]

Identifier: 150005161

GI number: 150005161

Start: 3340859

End: 3341875

Strand: Reverse

Name: apbE [H]

Synonym: BVU_2630

Alternate gene names: 150005161

Gene position: 3341875-3340859 (Counterclockwise)

Preceding gene: 150005162

Following gene: 150005159

Centisome position: 64.73

GC content: 40.12

Gene sequence:

>1017_bases
ATGAATAAAAAAAAATTAGCATGGCAATTGCCGTTTCTAGCAATTCTGATAATAGGAACAATTATTATTCTAAAAAAGCA
AGCCCCTTTCCGTACGGATGAAGGCTTTGTATTCGGTACAGTATACAAAATCACCTACCAGTCCGAAGATAATCTGAAAG
AAGAAATAGAAACTGAACTGAAGAAAGTGGACAACTCCCTCTCTCCTTTCAATCCGAATTCCGTAATCACCCGCGTAAAC
CACAATGAAAAGACGGAAGTTGACAGTTTTTTCGTACATGTATTCCATCTCTCAAAGAAAATATCCGATGAAACCCATGG
AGCCTTTGATATCACCGTCGCACCTTTGGTAAATGCATGGGGATTCGGATTCAAAAAATCTACAGGAGTGGATTCACTGA
TAGTGGACAGCTTACGCCAAATGATAGGATATCAAAAAATAGATTTACAGAACAACCGGATTATGAAAAAAGACCCTCGT
ATAATGCTGGATTGCAGCGCTATAGCCAAGGGATTCGGAGTAGATGCAGTAGCAAGGCTATTGGAACGTAAAGGGATAAA
AAACTATATGGTAGATATAGGCGGTGAAGTAGTGGTAAGAGGCAAAAATTCCAAAATGAACGCTTGGCGTATCGGCATAA
ACAAACCTGTAGATGACTCCTTGTCAGTAAACCAGGAATTACAGACTGTGCTTGCCATTAGTGATGTAGGTATGGCAACA
TCAGGGAATTATCGTAATTTCTATTACAAAGGTGGAAAGAAATATGCACATACCATAGACCCACGCACCGGATATCCAGT
ACAACATAGTATCCTGTCATCCACTGTCATTGCAAAAGATTGCGCCAGTGCCGATGCATACGCCACTGCTTTCATGGTGA
TGGGACTGGACTCAGCCAAAGCATTTTGTGAAGCACATCCTGAACTGGATGCTTATTTTATTTGCTCAGGCGAAGGAGAT
AAATATGAAACTTATTTTACAGACGGAATGAAGAAATTCATCATCAACGAGAAATAG

Upstream 100 bases:

>100_bases
TTCAACCTGCGCATGGAATTATGGGGAGGTTTGGTTTTGATTATTATCGGCGTTAAAATTTTAATAGAACATTTATTTCT
TTCTTAACTAAAATACAGGT

Downstream 100 bases:

>100_bases
ACACCAAAGGAGCGTATCAAAATATCAAACAGCTACCATTCAAATGGAATGATACCGGAACTCACCACAGATTAATATCC
ATTCAACACAAAGCAGTTGG

Product: thiamine biosynthesis lipoprotein apbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MNKKKLAWQLPFLAILIIGTIIILKKQAPFRTDEGFVFGTVYKITYQSEDNLKEEIETELKKVDNSLSPFNPNSVITRVN
HNEKTEVDSFFVHVFHLSKKISDETHGAFDITVAPLVNAWGFGFKKSTGVDSLIVDSLRQMIGYQKIDLQNNRIMKKDPR
IMLDCSAIAKGFGVDAVARLLERKGIKNYMVDIGGEVVVRGKNSKMNAWRIGINKPVDDSLSVNQELQTVLAISDVGMAT
SGNYRNFYYKGGKKYAHTIDPRTGYPVQHSILSSTVIAKDCASADAYATAFMVMGLDSAKAFCEAHPELDAYFICSGEGD
KYETYFTDGMKKFIINEK

Sequences:

>Translated_338_residues
MNKKKLAWQLPFLAILIIGTIIILKKQAPFRTDEGFVFGTVYKITYQSEDNLKEEIETELKKVDNSLSPFNPNSVITRVN
HNEKTEVDSFFVHVFHLSKKISDETHGAFDITVAPLVNAWGFGFKKSTGVDSLIVDSLRQMIGYQKIDLQNNRIMKKDPR
IMLDCSAIAKGFGVDAVARLLERKGIKNYMVDIGGEVVVRGKNSKMNAWRIGINKPVDDSLSVNQELQTVLAISDVGMAT
SGNYRNFYYKGGKKYAHTIDPRTGYPVQHSILSSTVIAKDCASADAYATAFMVMGLDSAKAFCEAHPELDAYFICSGEGD
KYETYFTDGMKKFIINEK
>Mature_338_residues
MNKKKLAWQLPFLAILIIGTIIILKKQAPFRTDEGFVFGTVYKITYQSEDNLKEEIETELKKVDNSLSPFNPNSVITRVN
HNEKTEVDSFFVHVFHLSKKISDETHGAFDITVAPLVNAWGFGFKKSTGVDSLIVDSLRQMIGYQKIDLQNNRIMKKDPR
IMLDCSAIAKGFGVDAVARLLERKGIKNYMVDIGGEVVVRGKNSKMNAWRIGINKPVDDSLSVNQELQTVLAISDVGMAT
SGNYRNFYYKGGKKYAHTIDPRTGYPVQHSILSSTVIAKDCASADAYATAFMVMGLDSAKAFCEAHPELDAYFICSGEGD
KYETYFTDGMKKFIINEK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=319, Percent_Identity=35.1097178683386, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37819; Mature: 37819

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKKLAWQLPFLAILIIGTIIILKKQAPFRTDEGFVFGTVYKITYQSEDNLKEEIETEL
CCCCCEEEHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
KKVDNSLSPFNPNSVITRVNHNEKTEVDSFFVHVFHLSKKISDETHGAFDITVAPLVNAW
HHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
GFGFKKSTGVDSLIVDSLRQMIGYQKIDLQNNRIMKKDPRIMLDCSAIAKGFGVDAVARL
CCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCEEECCCCCEEEEHHHHHCCCCHHHHHHH
LERKGIKNYMVDIGGEVVVRGKNSKMNAWRIGINKPVDDSLSVNQELQTVLAISDVGMAT
HHHCCCHHHHEECCCEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
SGNYRNFYYKGGKKYAHTIDPRTGYPVQHSILSSTVIAKDCASADAYATAFMVMGLDSAK
CCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHH
AFCEAHPELDAYFICSGEGDKYETYFTDGMKKFIINEK
HHHHCCCCCCEEEEECCCCCCEEHHHHCCHHHHHCCCC
>Mature Secondary Structure
MNKKKLAWQLPFLAILIIGTIIILKKQAPFRTDEGFVFGTVYKITYQSEDNLKEEIETEL
CCCCCEEEHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
KKVDNSLSPFNPNSVITRVNHNEKTEVDSFFVHVFHLSKKISDETHGAFDITVAPLVNAW
HHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
GFGFKKSTGVDSLIVDSLRQMIGYQKIDLQNNRIMKKDPRIMLDCSAIAKGFGVDAVARL
CCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCEEECCCCCEEEEHHHHHCCCCHHHHHHH
LERKGIKNYMVDIGGEVVVRGKNSKMNAWRIGINKPVDDSLSVNQELQTVLAISDVGMAT
HHHCCCHHHHEECCCEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
SGNYRNFYYKGGKKYAHTIDPRTGYPVQHSILSSTVIAKDCASADAYATAFMVMGLDSAK
CCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHH
AFCEAHPELDAYFICSGEGDKYETYFTDGMKKFIINEK
HHHHCCCCCCEEEEECCCCCCEEHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]