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The map label for this gene is truA [H]

Identifier: 150005081

GI number: 150005081

Start: 3248250

End: 3249053

Strand: Direct

Name: truA [H]

Synonym: BVU_2546

Alternate gene names: 150005081

Gene position: 3248250-3249053 (Clockwise)

Preceding gene: 150005080

Following gene: 150005084

Centisome position: 62.91

GC content: 43.53

Gene sequence:

>804_bases
TTGAATTTTCACTTGGTCGGAGGCTTTACGATGCGGTATTTCATATATTTGGCTTATGATGGAACGAATTATCACGGTTG
GCAGATTCAGCCCAATGGTGACAGTGTACAGGAAACATTAATGCGTGCTCTTTCTACTTTTCTCCGTCAAGAAATAGAGG
TAGTGGGAGCGGGACGTACTGATGCCGGTGTGCATGCGCGTCTCATGATCGCGCATTTTGATTATGACCGGGAGTTGGAT
TGTGTTCAGATAACGGAGAAATTGAATCGCTTGCTTCCTCCCGATATAGCGGTTTACGCTGTAAAGCGGGTGAAGGATGA
TGCGCATGCCCGTTTTGACGCGACTTATCGTACTTATAAGTATTATGTGACTACCCGGAAAGATCCGTTTAACCGTCACT
ATGCCTGGAGGCTTTTTAATTCATTGGATTTTGAGAAAATGAATGAGGCTGCCCGTGTATTGTTTGATTATACTGATTTT
ACCAGTTTCAGTAAATTGCATACTGATGTGAAGACGAATAATTGCCGGATTATGCATGCTGAATGGACGCAGGTGAGCGG
GACAGAATGGGTATTTATTATTAAAGCTGACCGTTTTTTACGGAATATGGTAAGGGCTGTGGTAGGTACGTTGGTAGAAG
TGGGACGTGGTAAAATGACTGTGGATGAGTTCAGGAAAGTGATAGAGGATAAAGATCGTTGTAGTGCCGGGAGTTCTGTT
CCGGGACATGCCTTGTTTTTGGTAGATGTGGGATATCCGGAAGAACTGTTTATTGTTTCTCCGTCAGATATTTCCAATAT
CTGA

Upstream 100 bases:

>100_bases
GTATTGGCCGAGGTACTCCGTGATCTTCGGAAATCGGACAAATGCTATATTAAATATGAGTTGAAACAGGGATGTTTTCA
TATAACAGCCCGCTGATTTA

Downstream 100 bases:

>100_bases
CAGGCATATCTTGCCGGTGTTTTCTGGGATTCCAACGTTCTTTGATGCAAATGGACTTGAAATATGTTTTCCAAAGTTTC
TGAAACAATTTCTCATCATT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNFHLVGGFTMRYFIYLAYDGTNYHGWQIQPNGDSVQETLMRALSTFLRQEIEVVGAGRTDAGVHARLMIAHFDYDRELD
CVQITEKLNRLLPPDIAVYAVKRVKDDAHARFDATYRTYKYYVTTRKDPFNRHYAWRLFNSLDFEKMNEAARVLFDYTDF
TSFSKLHTDVKTNNCRIMHAEWTQVSGTEWVFIIKADRFLRNMVRAVVGTLVEVGRGKMTVDEFRKVIEDKDRCSAGSSV
PGHALFLVDVGYPEELFIVSPSDISNI

Sequences:

>Translated_267_residues
MNFHLVGGFTMRYFIYLAYDGTNYHGWQIQPNGDSVQETLMRALSTFLRQEIEVVGAGRTDAGVHARLMIAHFDYDRELD
CVQITEKLNRLLPPDIAVYAVKRVKDDAHARFDATYRTYKYYVTTRKDPFNRHYAWRLFNSLDFEKMNEAARVLFDYTDF
TSFSKLHTDVKTNNCRIMHAEWTQVSGTEWVFIIKADRFLRNMVRAVVGTLVEVGRGKMTVDEFRKVIEDKDRCSAGSSV
PGHALFLVDVGYPEELFIVSPSDISNI
>Mature_267_residues
MNFHLVGGFTMRYFIYLAYDGTNYHGWQIQPNGDSVQETLMRALSTFLRQEIEVVGAGRTDAGVHARLMIAHFDYDRELD
CVQITEKLNRLLPPDIAVYAVKRVKDDAHARFDATYRTYKYYVTTRKDPFNRHYAWRLFNSLDFEKMNEAARVLFDYTDF
TSFSKLHTDVKTNNCRIMHAEWTQVSGTEWVFIIKADRFLRNMVRAVVGTLVEVGRGKMTVDEFRKVIEDKDRCSAGSSV
PGHALFLVDVGYPEELFIVSPSDISNI

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=30.3249097472924, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=37.7510040160643, Blast_Score=147, Evalue=9e-37,
Organism=Drosophila melanogaster, GI116007752, Length=301, Percent_Identity=26.9102990033223, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30896; Mature: 30896

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFHLVGGFTMRYFIYLAYDGTNYHGWQIQPNGDSVQETLMRALSTFLRQEIEVVGAGRT
CCEEEEECEEEEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCC
DAGVHARLMIAHFDYDRELDCVQITEKLNRLLPPDIAVYAVKRVKDDAHARFDATYRTYK
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHEECCEEEEEE
YYVTTRKDPFNRHYAWRLFNSLDFEKMNEAARVLFDYTDFTSFSKLHTDVKTNNCRIMHA
EEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHEEECCCCCHHHHHHCCEECCCEEEEEE
EWTQVSGTEWVFIIKADRFLRNMVRAVVGTLVEVGRGKMTVDEFRKVIEDKDRCSAGSSV
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCC
PGHALFLVDVGYPEELFIVSPSDISNI
CCCEEEEEECCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MNFHLVGGFTMRYFIYLAYDGTNYHGWQIQPNGDSVQETLMRALSTFLRQEIEVVGAGRT
CCEEEEECEEEEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCC
DAGVHARLMIAHFDYDRELDCVQITEKLNRLLPPDIAVYAVKRVKDDAHARFDATYRTYK
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHEECCEEEEEE
YYVTTRKDPFNRHYAWRLFNSLDFEKMNEAARVLFDYTDFTSFSKLHTDVKTNNCRIMHA
EEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHEEECCCCCHHHHHHCCEECCCEEEEEE
EWTQVSGTEWVFIIKADRFLRNMVRAVVGTLVEVGRGKMTVDEFRKVIEDKDRCSAGSSV
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCC
PGHALFLVDVGYPEELFIVSPSDISNI
CCCEEEEEECCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]