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The map label for this gene is smpB

Identifier: 150005062

GI number: 150005062

Start: 3229132

End: 3229584

Strand: Direct

Name: smpB

Synonym: BVU_2527

Alternate gene names: 150005062

Gene position: 3229132-3229584 (Clockwise)

Preceding gene: 150005061

Following gene: 150005063

Centisome position: 62.54

GC content: 43.27

Gene sequence:

>453_bases
ATGAAACCGACTCCTATTAATATAAAGAATAAGCGTGCATCGTTCGATTATGAATTTATCGATACGTACACGGCAGGTAT
CGTGCTCACGGGAACCGAGATAAAGTCCATCCGTTTGGGCAAAGCCAGTCTGGTAGATACCTATTGTTATTTTGTGCAAG
GCGAGTTGTGGGTGAAGAATATGCACATAGCCGAATACTTTTACGGCTCATACAATAACCATTCTGCCCGTCGCGAACGT
AAATTGTTGTTGAATAAGAAAGAACTGCGGAAATTGGAAGAAGCCGGAAAGAACCCCGGTTTTACGATTGTCCCTGTCCG
TCTGTTTATTAATGAGAAAGGACTGGCGAAGCTGGTTGTCGCATTGGCTAAAGGTAAGAAACAATATGATAAGCGCGAGT
CATTGAAAGAGAAGGACGACCGTCGCGAAATGGACCGTATGTTTAAAAGGTAA

Upstream 100 bases:

>100_bases
AGAAACTGACACGATACACCTTGATTGCTTCAGTTATTATAATTGTTTGTCCGGCATTGATTGCCGCTGTGTTTAATGAG
TTATCTGTTATTTTAAATTA

Downstream 100 bases:

>100_bases
GTGAAATCCGTGTTATCCGTACCCCAAAAATAGACTTATGGCTACATTAAAACAACTAATTGACGAGAGAGTTCTCATTC
TTGATGGTGCAATGGGAACT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKPTPINIKNKRASFDYEFIDTYTAGIVLTGTEIKSIRLGKASLVDTYCYFVQGELWVKNMHIAEYFYGSYNNHSARRER
KLLLNKKELRKLEEAGKNPGFTIVPVRLFINEKGLAKLVVALAKGKKQYDKRESLKEKDDRREMDRMFKR

Sequences:

>Translated_150_residues
MKPTPINIKNKRASFDYEFIDTYTAGIVLTGTEIKSIRLGKASLVDTYCYFVQGELWVKNMHIAEYFYGSYNNHSARRER
KLLLNKKELRKLEEAGKNPGFTIVPVRLFINEKGLAKLVVALAKGKKQYDKRESLKEKDDRREMDRMFKR
>Mature_150_residues
MKPTPINIKNKRASFDYEFIDTYTAGIVLTGTEIKSIRLGKASLVDTYCYFVQGELWVKNMHIAEYFYGSYNNHSARRER
KLLLNKKELRKLEEAGKNPGFTIVPVRLFINEKGLAKLVVALAKGKKQYDKRESLKEKDDRREMDRMFKR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=150, Percent_Identity=35.3333333333333, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_BACV8 (A6L3C0)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299806.1
- ProteinModelPortal:   A6L3C0
- SMR:   A6L3C0
- STRING:   A6L3C0
- GeneID:   5303491
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2527
- NMPDR:   fig|435590.6.peg.2369
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17572; Mature: 17572

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTPINIKNKRASFDYEFIDTYTAGIVLTGTEIKSIRLGKASLVDTYCYFVQGELWVKN
CCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCEEEEECCCHHHHHHHHHHHCCEEEEEH
MHIAEYFYGSYNNHSARRERKLLLNKKELRKLEEAGKNPGFTIVPVRLFINEKGLAKLVV
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHH
ALAKGKKQYDKRESLKEKDDRREMDRMFKR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPTPINIKNKRASFDYEFIDTYTAGIVLTGTEIKSIRLGKASLVDTYCYFVQGELWVKN
CCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCEEEEECCCHHHHHHHHHHHCCEEEEEH
MHIAEYFYGSYNNHSARRERKLLLNKKELRKLEEAGKNPGFTIVPVRLFINEKGLAKLVV
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHH
ALAKGKKQYDKRESLKEKDDRREMDRMFKR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA