Click here to switch to the map view.

The map label for this gene is ply [H]

Identifier: 150004997

GI number: 150004997

Start: 3162275

End: 3163822

Strand: Direct

Name: ply [H]

Synonym: BVU_2462

Alternate gene names: 150004997

Gene position: 3162275-3163822 (Clockwise)

Preceding gene: 150004996

Following gene: 150004998

Centisome position: 61.25

GC content: 35.72

Gene sequence:

>1548_bases
ATGAGAATTTTAAGATATACTCTGTTTTTATTTGCTATAGGATTAATTTCATGTGAACAAGAATTTGACAAAAAAGAACA
GCAATCTGATTGCTGTTCTTTTCAAAATCTACCTAAGTATCCCGAACAAGTGGTGAATATAAATGTGCATAACTATATTC
CTGATATGGGAGATAAGGTTTTATCTTCTCCTTCGCATATCCATACAAGAGCTTCAGAAATCATTTCTGAGACATTATGG
GATAATGGGTTTGGTAAAACAATTCATGCACAGGCAGATGAGTCGATAGTTTTGGATAATCTGAATCGATATATTTATCC
TGGTTCTATCTTGGATGGGGCAAGCGTTGCCAATCAGGATTATAAAACAATCAGTGTCCACTATAAGCCTATAAATGTCT
CTGTGTCCTTTCCTGCCCAAAAAGTTACAGGTGTACTTGAAAAGCCCTCTTTGTCTTCATGTAGGCAGTTAGTAATGGAT
TTGATGCATCAGAAAGGTATAGGGCAACAATCAGCTTCCGTACACTTTGATATACACCGTTTTACATCTTATGATGAGCT
GAAAATGACTTTTGGATCAAATGCCAATACGAGCTTTTTATTTTGGGGGTCGTCATCTTCCCAACAAGAACATAAGGAAC
GAATTAGTAAAAGTAGTGGCTTATATATAAGGTTTATTCAGAAATATTTTACGATTGATATGGATATTCCTGAGAAGAGT
TTTATTGAAGGTAGTATCAATGCCCAATACTCTCCTGTCTATGTGAGCTCCATAGCTTATGGAAGGGTCGGCATATTAAC
TCTTGAAACCGATGAAATATACGAGAATGCGGAGTCCATTGTAAAGAAGGCCGTTAATGGCTTTTTATATAACAAAAAAG
AATTTCTGACAGTTGAAGAAAAGGGCTTTTTTGATGAGGCCGGAATGAAAGTATATGTAGGGGGAGGAAATGGTGACAGT
GGTGTAAAGACCCTTACCGGATTTGACGATTTTATCAAATTTATAAGTGAAGGAGGGCATTTTTCTGCAGAGACACCTGG
TAAACCTATATTTTGTAGCTTTGCGTATTTGTCGGATCATTCACCTTATAAAGTTAAATTTAAAATTGATATTGATAGCG
ATCCTGTATATGCAAGAATTGAATATCGGAATCTAAAAAATGACTCCTATGTGGGTAGATCTTTACAAAGAGAAAAGATA
ATTGGCGATGTACATCTTGCCTTTTATGCTGATAGAACGGCTAAGATTCCGACAGTTGCTCCAAGATACATTTCTTTTAA
TATTGTAGAACATAGCAGACATTATTTGAAGGCTCGAATACCAAGAACAGAAAAGGATGATACAACAGTAGAAGAATTTG
TCAAGAGTAATAATAGCCGTGGGACTGAATTACAATTAAAACATGATTTGTTGCTCACAGAATTTGTTTGGACGCATAAT
AACCCAAGAAGAACTCGATATGAACAAAACACTTTCAGGTATAGTCTTAGTGAAGGTGGTTTTTACAAAATTCTCCCTGC
TATAAATGAGCCAAAGAAGTGGCGATAA

Upstream 100 bases:

>100_bases
GGGGGATGCCATATTCTATACTGCCAGTTATCTGTCTGATAATTCTCCTTATTATTCAAAATTCAAAATTCATTTAAAAA
ATCAACAATAATCTGGAGCT

Downstream 100 bases:

>100_bases
TTTGAAGTTGGCGCTCCTTGTCGGACAAATAAATAAGAGATATGGTATTAAAAGATAAATTGGTTATGAAAAAAGTATGT
ATGTTACTTCTTTTGTTATT

Product: putative hemolysin

Products: NA

Alternate protein names: Thiol-activated cytolysin [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MRILRYTLFLFAIGLISCEQEFDKKEQQSDCCSFQNLPKYPEQVVNINVHNYIPDMGDKVLSSPSHIHTRASEIISETLW
DNGFGKTIHAQADESIVLDNLNRYIYPGSILDGASVANQDYKTISVHYKPINVSVSFPAQKVTGVLEKPSLSSCRQLVMD
LMHQKGIGQQSASVHFDIHRFTSYDELKMTFGSNANTSFLFWGSSSSQQEHKERISKSSGLYIRFIQKYFTIDMDIPEKS
FIEGSINAQYSPVYVSSIAYGRVGILTLETDEIYENAESIVKKAVNGFLYNKKEFLTVEEKGFFDEAGMKVYVGGGNGDS
GVKTLTGFDDFIKFISEGGHFSAETPGKPIFCSFAYLSDHSPYKVKFKIDIDSDPVYARIEYRNLKNDSYVGRSLQREKI
IGDVHLAFYADRTAKIPTVAPRYISFNIVEHSRHYLKARIPRTEKDDTTVEEFVKSNNSRGTELQLKHDLLLTEFVWTHN
NPRRTRYEQNTFRYSLSEGGFYKILPAINEPKKWR

Sequences:

>Translated_515_residues
MRILRYTLFLFAIGLISCEQEFDKKEQQSDCCSFQNLPKYPEQVVNINVHNYIPDMGDKVLSSPSHIHTRASEIISETLW
DNGFGKTIHAQADESIVLDNLNRYIYPGSILDGASVANQDYKTISVHYKPINVSVSFPAQKVTGVLEKPSLSSCRQLVMD
LMHQKGIGQQSASVHFDIHRFTSYDELKMTFGSNANTSFLFWGSSSSQQEHKERISKSSGLYIRFIQKYFTIDMDIPEKS
FIEGSINAQYSPVYVSSIAYGRVGILTLETDEIYENAESIVKKAVNGFLYNKKEFLTVEEKGFFDEAGMKVYVGGGNGDS
GVKTLTGFDDFIKFISEGGHFSAETPGKPIFCSFAYLSDHSPYKVKFKIDIDSDPVYARIEYRNLKNDSYVGRSLQREKI
IGDVHLAFYADRTAKIPTVAPRYISFNIVEHSRHYLKARIPRTEKDDTTVEEFVKSNNSRGTELQLKHDLLLTEFVWTHN
NPRRTRYEQNTFRYSLSEGGFYKILPAINEPKKWR
>Mature_515_residues
MRILRYTLFLFAIGLISCEQEFDKKEQQSDCCSFQNLPKYPEQVVNINVHNYIPDMGDKVLSSPSHIHTRASEIISETLW
DNGFGKTIHAQADESIVLDNLNRYIYPGSILDGASVANQDYKTISVHYKPINVSVSFPAQKVTGVLEKPSLSSCRQLVMD
LMHQKGIGQQSASVHFDIHRFTSYDELKMTFGSNANTSFLFWGSSSSQQEHKERISKSSGLYIRFIQKYFTIDMDIPEKS
FIEGSINAQYSPVYVSSIAYGRVGILTLETDEIYENAESIVKKAVNGFLYNKKEFLTVEEKGFFDEAGMKVYVGGGNGDS
GVKTLTGFDDFIKFISEGGHFSAETPGKPIFCSFAYLSDHSPYKVKFKIDIDSDPVYARIEYRNLKNDSYVGRSLQREKI
IGDVHLAFYADRTAKIPTVAPRYISFNIVEHSRHYLKARIPRTEKDDTTVEEFVKSNNSRGTELQLKHDLLLTEFVWTHN
NPRRTRYEQNTFRYSLSEGGFYKILPAINEPKKWR

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 58811; Mature: 58811

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILRYTLFLFAIGLISCEQEFDKKEQQSDCCSFQNLPKYPEQVVNINVHNYIPDMGDKV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHHCCCCCCHHH
LSSPSHIHTRASEIISETLWDNGFGKTIHAQADESIVLDNLNRYIYPGSILDGASVANQD
HCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCEECCCCCCCCCHHCCCC
YKTISVHYKPINVSVSFPAQKVTGVLEKPSLSSCRQLVMDLMHQKGIGQQSASVHFDIHR
CEEEEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
FTSYDELKMTFGSNANTSFLFWGSSSSQQEHKERISKSSGLYIRFIQKYFTIDMDIPEKS
CCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEHHHHEEEECCCCHH
FIEGSINAQYSPVYVSSIAYGRVGILTLETDEIYENAESIVKKAVNGFLYNKKEFLTVEE
HCCCCCCCEECCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHEECCCCEEEEEC
KGFFDEAGMKVYVGGGNGDSGVKTLTGFDDFIKFISEGGHFSAETPGKPIFCSFAYLSDH
CCCCCCCCCEEEEECCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
SPYKVKFKIDIDSDPVYARIEYRNLKNDSYVGRSLQREKIIGDVHLAFYADRTAKIPTVA
CCEEEEEEEEECCCCEEEEEEEECCCCCCHHCCHHHHHHHHCEEEEEEEECCCCCCCCCC
PRYISFNIVEHSRHYLKARIPRTEKDDTTVEEFVKSNNSRGTELQLKHDLLLTEFVWTHN
CCEEEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHEEEEHEEECC
NPRRTRYEQNTFRYSLSEGGFYKILPAINEPKKWR
CCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MRILRYTLFLFAIGLISCEQEFDKKEQQSDCCSFQNLPKYPEQVVNINVHNYIPDMGDKV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHHCCCCCCHHH
LSSPSHIHTRASEIISETLWDNGFGKTIHAQADESIVLDNLNRYIYPGSILDGASVANQD
HCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCEECCCCCCCCCHHCCCC
YKTISVHYKPINVSVSFPAQKVTGVLEKPSLSSCRQLVMDLMHQKGIGQQSASVHFDIHR
CEEEEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
FTSYDELKMTFGSNANTSFLFWGSSSSQQEHKERISKSSGLYIRFIQKYFTIDMDIPEKS
CCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEHHHHEEEECCCCHH
FIEGSINAQYSPVYVSSIAYGRVGILTLETDEIYENAESIVKKAVNGFLYNKKEFLTVEE
HCCCCCCCEECCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHEECCCCEEEEEC
KGFFDEAGMKVYVGGGNGDSGVKTLTGFDDFIKFISEGGHFSAETPGKPIFCSFAYLSDH
CCCCCCCCCEEEEECCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
SPYKVKFKIDIDSDPVYARIEYRNLKNDSYVGRSLQREKIIGDVHLAFYADRTAKIPTVA
CCEEEEEEEEECCCCEEEEEEEECCCCCCHHCCHHHHHHHHCEEEEEEEECCCCCCCCCC
PRYISFNIVEHSRHYLKARIPRTEKDDTTVEEFVKSNNSRGTELQLKHDLLLTEFVWTHN
CCEEEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHEEEEHEEECC
NPRRTRYEQNTFRYSLSEGGFYKILPAINEPKKWR
CCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3552992 [H]