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The map label for this gene is ply [H]

Identifier: 150004996

GI number: 150004996

Start: 3161159

End: 3162265

Strand: Direct

Name: ply [H]

Synonym: BVU_2461

Alternate gene names: 150004996

Gene position: 3161159-3162265 (Clockwise)

Preceding gene: 150004995

Following gene: 150004997

Centisome position: 61.22

GC content: 31.89

Gene sequence:

>1107_bases
ATGAAAAAGTGTTTATATCTATCTGTTTTTTTTATTGCTTTTTTTTCTTCGTGTTCCGATAACGAATTGGAAGAAATGTC
TAATTCAGTTCCCGTTTCAAAATATCCCCAAAAAGTTATAGATATTTCAATGGAGAGCCCTGAAAGTTTTTCACATTTTA
GTAATATTTCTATTACTCGTTCTTCTGAATTATTAAAGGACAGTGTGTGGAGTGATGGAAAAGGGCATACGTTAATTTAT
GAAACAAATGAACATGTTCCGACTCAAGAGTTAATGAGATATATTTATTTAGGTTCAATTCTTCAGGGAGGTTCTATTGA
AAAACAACGTTTTGTTCCTGTTTTAAAACCTGTTGATCCAATAACAATATCCTATTCTTTTCCTGCCCGTTGGGTGACAG
ACATAATCATGAAACCAAGTCTGTCAGCACAAAGGCAATCTTTACAGAACATAATGAATAAAGAAGGAATGGAAGGTAAA
CAATTAGGCTCTTTTAGCTATAATATGCGACAATTTACTTATTTTGAAGAGCTTAAATTAGCTTTTGGGGCAAATGTGAA
TATTGCACGTATTCTTGATATTGATATTAGTGTTGATAAAGGAAAAATCAGAAGAAAAACTGGATTGTTTGCGAAAATAA
TACAACGGAATTATACAGTTGACATGGATCTTCCTATTGATGGAAATCTGCTTTTAAATCATGATGAAATAAATAATATA
GGTAGATACGATCCGGTATATATTAGTTCTATTACTTATGGAAGGATGGCTCTAATTTCTATGGAATCTTTTGAAAGTTA
TGATAAGTTAAGAGTTGCGTTACAAGTGGCTTTACAGGCAAAAGTCATAAATGGGGAATTAGACTTTAGTCTTGAGCAAA
AAAAAATTCTCAAAGAAGCGGAAATAAATGTTGTAGTGTATAACGGAGAAGGTGAAGGCACTGTTAAAACTATTAAAGGT
TGGGATGAGTTTCAGAAATTCATAATCCAAGGAGGACGATTTTCTAAAGATTTGCCGGGGGATGCCATATTCTATACTGC
CAGTTATCTGTCTGATAATTCTCCTTATTATTCAAAATTCAAAATTCATTTAAAAAATCAACAATAA

Upstream 100 bases:

>100_bases
AAACTCTTGGTGAGGATTATTATAGACCTTCTCGTAGCGCATTTAGAAGTGGTACATTTGATTTTTAACATACCTATTGT
AAATTTAAATTATAAGTATT

Downstream 100 bases:

>100_bases
TCTGGAGCTATGAGAATTTTAAGATATACTCTGTTTTTATTTGCTATAGGATTAATTTCATGTGAACAAGAATTTGACAA
AAAAGAACAGCAATCTGATT

Product: putative hemolysin

Products: NA

Alternate protein names: Thiol-activated cytolysin [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKKCLYLSVFFIAFFSSCSDNELEEMSNSVPVSKYPQKVIDISMESPESFSHFSNISITRSSELLKDSVWSDGKGHTLIY
ETNEHVPTQELMRYIYLGSILQGGSIEKQRFVPVLKPVDPITISYSFPARWVTDIIMKPSLSAQRQSLQNIMNKEGMEGK
QLGSFSYNMRQFTYFEELKLAFGANVNIARILDIDISVDKGKIRRKTGLFAKIIQRNYTVDMDLPIDGNLLLNHDEINNI
GRYDPVYISSITYGRMALISMESFESYDKLRVALQVALQAKVINGELDFSLEQKKILKEAEINVVVYNGEGEGTVKTIKG
WDEFQKFIIQGGRFSKDLPGDAIFYTASYLSDNSPYYSKFKIHLKNQQ

Sequences:

>Translated_368_residues
MKKCLYLSVFFIAFFSSCSDNELEEMSNSVPVSKYPQKVIDISMESPESFSHFSNISITRSSELLKDSVWSDGKGHTLIY
ETNEHVPTQELMRYIYLGSILQGGSIEKQRFVPVLKPVDPITISYSFPARWVTDIIMKPSLSAQRQSLQNIMNKEGMEGK
QLGSFSYNMRQFTYFEELKLAFGANVNIARILDIDISVDKGKIRRKTGLFAKIIQRNYTVDMDLPIDGNLLLNHDEINNI
GRYDPVYISSITYGRMALISMESFESYDKLRVALQVALQAKVINGELDFSLEQKKILKEAEINVVVYNGEGEGTVKTIKG
WDEFQKFIIQGGRFSKDLPGDAIFYTASYLSDNSPYYSKFKIHLKNQQ
>Mature_368_residues
MKKCLYLSVFFIAFFSSCSDNELEEMSNSVPVSKYPQKVIDISMESPESFSHFSNISITRSSELLKDSVWSDGKGHTLIY
ETNEHVPTQELMRYIYLGSILQGGSIEKQRFVPVLKPVDPITISYSFPARWVTDIIMKPSLSAQRQSLQNIMNKEGMEGK
QLGSFSYNMRQFTYFEELKLAFGANVNIARILDIDISVDKGKIRRKTGLFAKIIQRNYTVDMDLPIDGNLLLNHDEINNI
GRYDPVYISSITYGRMALISMESFESYDKLRVALQVALQAKVINGELDFSLEQKKILKEAEINVVVYNGEGEGTVKTIKG
WDEFQKFIIQGGRFSKDLPGDAIFYTASYLSDNSPYYSKFKIHLKNQQ

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 42002; Mature: 42002

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKCLYLSVFFIAFFSSCSDNELEEMSNSVPVSKYPQKVIDISMESPESFSHFSNISITR
CCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHCCHHHEEEECCCCHHHHHHCCEEEEC
SSELLKDSVWSDGKGHTLIYETNEHVPTQELMRYIYLGSILQGGSIEKQRFVPVLKPVDP
HHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHCCCCCC
ITISYSFPARWVTDIIMKPSLSAQRQSLQNIMNKEGMEGKQLGSFSYNMRQFTYFEELKL
EEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AFGANVNIARILDIDISVDKGKIRRKTGLFAKIIQRNYTVDMDLPIDGNLLLNHDEINNI
HHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECHHCCCC
GRYDPVYISSITYGRMALISMESFESYDKLRVALQVALQAKVINGELDFSLEQKKILKEA
CCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHC
EINVVVYNGEGEGTVKTIKGWDEFQKFIIQGGRFSKDLPGDAIFYTASYLSDNSPYYSKF
CEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCEEEE
KIHLKNQQ
EEEEECCC
>Mature Secondary Structure
MKKCLYLSVFFIAFFSSCSDNELEEMSNSVPVSKYPQKVIDISMESPESFSHFSNISITR
CCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHCCHHHEEEECCCCHHHHHHCCEEEEC
SSELLKDSVWSDGKGHTLIYETNEHVPTQELMRYIYLGSILQGGSIEKQRFVPVLKPVDP
HHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHCCCCCC
ITISYSFPARWVTDIIMKPSLSAQRQSLQNIMNKEGMEGKQLGSFSYNMRQFTYFEELKL
EEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AFGANVNIARILDIDISVDKGKIRRKTGLFAKIIQRNYTVDMDLPIDGNLLLNHDEINNI
HHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECHHCCCC
GRYDPVYISSITYGRMALISMESFESYDKLRVALQVALQAKVINGELDFSLEQKKILKEA
CCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHC
EINVVVYNGEGEGTVKTIKGWDEFQKFIIQGGRFSKDLPGDAIFYTASYLSDNSPYYSKF
CEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCEEEE
KIHLKNQQ
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3552992 [H]