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The map label for this gene is nfo

Identifier: 150004972

GI number: 150004972

Start: 3130956

End: 3131792

Strand: Direct

Name: nfo

Synonym: BVU_2436

Alternate gene names: 150004972

Gene position: 3130956-3131792 (Clockwise)

Preceding gene: 150004971

Following gene: 150004973

Centisome position: 60.64

GC content: 44.44

Gene sequence:

>837_bases
ATGAAAAAAATAGGTGCACATGTATCCGTAAGCGGGGGCGTGGAAATGGCCCCCGTTAATGCATTAGGGATAGGAGCGGA
CGCATTCGCTCTTTTTACAAAGAATCAGAGGCAATGGGTTGCAAAACCTTTGTCTGTCGAGAGTGTTACTCTGTTTAAAG
AAAACTGCGAGAAAGAAGGTTTTGATGCCCGGTATGTTCTTCCTCATGACAGTTATCTGATAAATTTGGGACATCCTGAC
GAGGAAGGGCTGGAGAAGAGTCGTGCAGCATTTTTGGACGAAATGCAGCGTTGCGAGCTTTTAGGATTGAAAATGCTGAA
TTTTCATCCCGGAAGTCATTTGAATAAGATATCCATAGAGAAGTGTCTGGACAGGATTGCCGAGTCTGTCAATATGACTT
TGGATAAAACGACCGGTGTGACTGCTGTGATTGAGAATACAGCCGGACAGGGAAGTAATGTGGGAAATGAATTCTGGCAT
TTGAGATATATTATAGATAAGGTGGAGGATAAATCTCGTGTGGGAGTTTGCCTGGATACTTGCCATACCTATACCGCCGG
TTATGATATTGTGAATGAATATGACCGGGTCTTTACCGAGTTTGATGAGGTAGTGGGGAGAAACTATTTGTGTGCCATTC
ACTTGAATGACTCAAAGAAACCATTGGGTAGCCGGGTAGACCGTCATGACAGTATCGGAAAGGGGCTGATTGGTATTGAT
TTCTTCCGCCGCTTTATGCAGGATTCCCGTTTTGACGATATGCCCGTTATTCTTGAAACTCCCGATGACACCATTTGGCG
GGACGAGATTAAAATGCTTCGCAGCTTTGAGTCATAG

Upstream 100 bases:

>100_bases
TAATGATGTGAACCGTGAAGCAAACCGTATAAACTTGAGTGCGCAATATAATTTCTAACTTATTTTTCCTATCTTTGTGT
CTATCAATAATGGAAAGAGA

Downstream 100 bases:

>100_bases
TTTGTGGCTGGGTGAAAACATATTTAAGGAATTCAGCTATCATTTTGCTTGTAAGGGCGAGGTTTGCTCGCCCGAAAATA
GGCTGATTTGCATATTCTCC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKKIGAHVSVSGGVEMAPVNALGIGADAFALFTKNQRQWVAKPLSVESVTLFKENCEKEGFDARYVLPHDSYLINLGHPD
EEGLEKSRAAFLDEMQRCELLGLKMLNFHPGSHLNKISIEKCLDRIAESVNMTLDKTTGVTAVIENTAGQGSNVGNEFWH
LRYIIDKVEDKSRVGVCLDTCHTYTAGYDIVNEYDRVFTEFDEVVGRNYLCAIHLNDSKKPLGSRVDRHDSIGKGLIGID
FFRRFMQDSRFDDMPVILETPDDTIWRDEIKMLRSFES

Sequences:

>Translated_278_residues
MKKIGAHVSVSGGVEMAPVNALGIGADAFALFTKNQRQWVAKPLSVESVTLFKENCEKEGFDARYVLPHDSYLINLGHPD
EEGLEKSRAAFLDEMQRCELLGLKMLNFHPGSHLNKISIEKCLDRIAESVNMTLDKTTGVTAVIENTAGQGSNVGNEFWH
LRYIIDKVEDKSRVGVCLDTCHTYTAGYDIVNEYDRVFTEFDEVVGRNYLCAIHLNDSKKPLGSRVDRHDSIGKGLIGID
FFRRFMQDSRFDDMPVILETPDDTIWRDEIKMLRSFES
>Mature_278_residues
MKKIGAHVSVSGGVEMAPVNALGIGADAFALFTKNQRQWVAKPLSVESVTLFKENCEKEGFDARYVLPHDSYLINLGHPD
EEGLEKSRAAFLDEMQRCELLGLKMLNFHPGSHLNKISIEKCLDRIAESVNMTLDKTTGVTAVIENTAGQGSNVGNEFWH
LRYIIDKVEDKSRVGVCLDTCHTYTAGYDIVNEYDRVFTEFDEVVGRNYLCAIHLNDSKKPLGSRVDRHDSIGKGLIGID
FFRRFMQDSRFDDMPVILETPDDTIWRDEIKMLRSFES

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=280, Percent_Identity=61.4285714285714, Blast_Score=352, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=49.4296577946768, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6322735, Length=281, Percent_Identity=42.3487544483986, Blast_Score=241, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_BACV8 (A6L330)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299716.1
- ProteinModelPortal:   A6L330
- SMR:   A6L330
- STRING:   A6L330
- GeneID:   5303400
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2436
- NMPDR:   fig|435590.6.peg.2292
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31400; Mature: 31400

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGAHVSVSGGVEMAPVNALGIGADAFALFTKNQRQWVAKPLSVESVTLFKENCEKEG
CCCCCCEEEECCCEEECCCCEEECCCHHEEEEECCCHHHHCCCCCCHHHHHHHHHHHCCC
FDARYVLPHDSYLINLGHPDEEGLEKSRAAFLDEMQRCELLGLKMLNFHPGSHLNKISIE
CCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHH
KCLDRIAESVNMTLDKTTGVTAVIENTAGQGSNVGNEFWHLRYIIDKVEDKSRVGVCLDT
HHHHHHHHHHCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
CHTYTAGYDIVNEYDRVFTEFDEVVGRNYLCAIHLNDSKKPLGSRVDRHDSIGKGLIGID
HHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCHHHHCCCCCHHH
FFRRFMQDSRFDDMPVILETPDDTIWRDEIKMLRSFES
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIGAHVSVSGGVEMAPVNALGIGADAFALFTKNQRQWVAKPLSVESVTLFKENCEKEG
CCCCCCEEEECCCEEECCCCEEECCCHHEEEEECCCHHHHCCCCCCHHHHHHHHHHHCCC
FDARYVLPHDSYLINLGHPDEEGLEKSRAAFLDEMQRCELLGLKMLNFHPGSHLNKISIE
CCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHH
KCLDRIAESVNMTLDKTTGVTAVIENTAGQGSNVGNEFWHLRYIIDKVEDKSRVGVCLDT
HHHHHHHHHHCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
CHTYTAGYDIVNEYDRVFTEFDEVVGRNYLCAIHLNDSKKPLGSRVDRHDSIGKGLIGID
HHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCHHHHCCCCCHHH
FFRRFMQDSRFDDMPVILETPDDTIWRDEIKMLRSFES
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA