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The map label for this gene is nuoI [C]

Identifier: 150004929

GI number: 150004929

Start: 3074421

End: 3075560

Strand: Direct

Name: nuoI [C]

Synonym: BVU_2392

Alternate gene names: 150004929

Gene position: 3074421-3075560 (Clockwise)

Preceding gene: 150004928

Following gene: 150004930

Centisome position: 59.55

GC content: 36.75

Gene sequence:

>1140_bases
ATGAAATTGATTTGTGAGAAAGAGTTATGTACGGGATGTGGACTGTGCGCCGCAAGGTGTCCCAAACATAGCATATCCCT
GGTTGCTGAAGAATTGGGACATCTTTATCCGTCCGTTGATCAGAAGAAATGCATTGATTGTGGGCTTTGTCAAAAAGCAT
GCCCATCGTTGCATGATACGGTATGTCTGTATCCGTCAGTGGCATACGCCGCATGGTCGAAAGACGAAGAAGACTATAGA
AGCAGCACTTCCGGCGGTATGGCTTCTGTATTGACTCATTATTTTTTAGCCAATGTAGGTATCGTTTATGGATGCACTGT
AATACCGGGCATTGAAATTAAGCATATCCGCATAGATAATCTTAAAGATGCATATAAATTGAAAGGCTCTAAATATGTGC
AAAGCAGTATCGTTGATGTCCTGTCACAAATTAGACAAGATGTAAAAGATGGAACAAACGTATTATTTATCGGGACACCT
TGTCAAGTGACAGCTGTCAAGCGAATGTATGAGGAACAACCTGATAATCTTTTCTTGGTAGACCTTATTTGTCATGGGGT
GCCTTCAAATAAATGGTTGGTTGACTATATAGCAAATACTCTCAAAATAAAAGCAGATAAAGTTTCTAGCATTGGTTTTC
GGTTATTTGAAGCTTTTAGTCTATGTGTATATAATGATGATAGGTTGATTTATAAGTCCGGAGATCTATGGACACACCGT
TATGAAGATTTATATTATAACACTTTTATTGACGGTTTTACCTATCGAGACAGTTGTTTTAATTGTCATTATGCCAAACC
GGAAAGAGTATCTGATATAACTATCGGAGATTTTTGGGGATTGGGTAATGAAACTGACGATAATTATATTCCCAAACATC
CGTATGGCATTTCCTGTGTTCTTCCTCTTACAGAAAAAGGTAAAAGACTCATTTCGATTGTAAAGGATAAGCTGAATATT
TACGAACGTCCAGTTACGGAAGCCATAAACGGTAATGACCAGCTCAGATTTCCCAAAAAGAAAAATCTAAGAATAAAGTT
TTTTAGAAGAATCCATAAATACATAGGAGTAGATGCTGCCTACAAACTATGTGTTTGTGATAAGATTGTAAAATATAAGA
TTAGAAAAATAATAAGATAG

Upstream 100 bases:

>100_bases
TTACCGCTATTGTCACTATGACCATTTCATGGTTTATCGGCTTGAAAAAGACTGAAAGGAATACGCTTATTTCCGTCGTA
AAAAACAAACTTCATAAAAA

Downstream 100 bases:

>100_bases
TAGCATTATAATATGGGAAATAGAAAAAAGAAATGTGGTATCGTTACATTTCATAGCTCACATAATTACGGCTCTGTTCT
ACAGGCTTATGCCATGGTAA

Product: F420H2-dehydrogenase, beta subunit

Products: NAD+; ubiquinol [C]

Alternate protein names: None

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKLICEKELCTGCGLCAARCPKHSISLVAEELGHLYPSVDQKKCIDCGLCQKACPSLHDTVCLYPSVAYAAWSKDEEDYR
SSTSGGMASVLTHYFLANVGIVYGCTVIPGIEIKHIRIDNLKDAYKLKGSKYVQSSIVDVLSQIRQDVKDGTNVLFIGTP
CQVTAVKRMYEEQPDNLFLVDLICHGVPSNKWLVDYIANTLKIKADKVSSIGFRLFEAFSLCVYNDDRLIYKSGDLWTHR
YEDLYYNTFIDGFTYRDSCFNCHYAKPERVSDITIGDFWGLGNETDDNYIPKHPYGISCVLPLTEKGKRLISIVKDKLNI
YERPVTEAINGNDQLRFPKKKNLRIKFFRRIHKYIGVDAAYKLCVCDKIVKYKIRKIIR

Sequences:

>Translated_379_residues
MKLICEKELCTGCGLCAARCPKHSISLVAEELGHLYPSVDQKKCIDCGLCQKACPSLHDTVCLYPSVAYAAWSKDEEDYR
SSTSGGMASVLTHYFLANVGIVYGCTVIPGIEIKHIRIDNLKDAYKLKGSKYVQSSIVDVLSQIRQDVKDGTNVLFIGTP
CQVTAVKRMYEEQPDNLFLVDLICHGVPSNKWLVDYIANTLKIKADKVSSIGFRLFEAFSLCVYNDDRLIYKSGDLWTHR
YEDLYYNTFIDGFTYRDSCFNCHYAKPERVSDITIGDFWGLGNETDDNYIPKHPYGISCVLPLTEKGKRLISIVKDKLNI
YERPVTEAINGNDQLRFPKKKNLRIKFFRRIHKYIGVDAAYKLCVCDKIVKYKIRKIIR
>Mature_379_residues
MKLICEKELCTGCGLCAARCPKHSISLVAEELGHLYPSVDQKKCIDCGLCQKACPSLHDTVCLYPSVAYAAWSKDEEDYR
SSTSGGMASVLTHYFLANVGIVYGCTVIPGIEIKHIRIDNLKDAYKLKGSKYVQSSIVDVLSQIRQDVKDGTNVLFIGTP
CQVTAVKRMYEEQPDNLFLVDLICHGVPSNKWLVDYIANTLKIKADKVSSIGFRLFEAFSLCVYNDDRLIYKSGDLWTHR
YEDLYYNTFIDGFTYRDSCFNCHYAKPERVSDITIGDFWGLGNETDDNYIPKHPYGISCVLPLTEKGKRLISIVKDKLNI
YERPVTEAINGNDQLRFPKKKNLRIKFFRRIHKYIGVDAAYKLCVCDKIVKYKIRKIIR

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 43193; Mature: 43193

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLICEKELCTGCGLCAARCPKHSISLVAEELGHLYPSVDQKKCIDCGLCQKACPSLHDT
CCCEECHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCC
VCLYPSVAYAAWSKDEEDYRSSTSGGMASVLTHYFLANVGIVYGCTVIPGIEIKHIRIDN
EEECCCHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHEECEECCCCEEEEEEECC
LKDAYKLKGSKYVQSSIVDVLSQIRQDVKDGTNVLFIGTPCQVTAVKRMYEEQPDNLFLV
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCEEEE
DLICHGVPSNKWLVDYIANTLKIKADKVSSIGFRLFEAFSLCVYNDDRLIYKSGDLWTHR
EHHHCCCCCCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEECHH
YEDLYYNTFIDGFTYRDSCFNCHYAKPERVSDITIGDFWGLGNETDDNYIPKHPYGISCV
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCEEE
LPLTEKGKRLISIVKDKLNIYERPVTEAINGNDQLRFPKKKNLRIKFFRRIHKYIGVDAA
EECCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCHHHH
YKLCVCDKIVKYKIRKIIR
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLICEKELCTGCGLCAARCPKHSISLVAEELGHLYPSVDQKKCIDCGLCQKACPSLHDT
CCCEECHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCC
VCLYPSVAYAAWSKDEEDYRSSTSGGMASVLTHYFLANVGIVYGCTVIPGIEIKHIRIDN
EEECCCHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHEECEECCCCEEEEEEECC
LKDAYKLKGSKYVQSSIVDVLSQIRQDVKDGTNVLFIGTPCQVTAVKRMYEEQPDNLFLV
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCEEEE
DLICHGVPSNKWLVDYIANTLKIKADKVSSIGFRLFEAFSLCVYNDDRLIYKSGDLWTHR
EHHHCCCCCCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEECHH
YEDLYYNTFIDGFTYRDSCFNCHYAKPERVSDITIGDFWGLGNETDDNYIPKHPYGISCV
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCEEE
LPLTEKGKRLISIVKDKLNIYERPVTEAINGNDQLRFPKKKNLRIKFFRRIHKYIGVDAA
EECCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCHHHH
YKLCVCDKIVKYKIRKIIR
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA