Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 150004902

GI number: 150004902

Start: 3044831

End: 3046756

Strand: Reverse

Name: nadE [H]

Synonym: BVU_2365

Alternate gene names: 150004902

Gene position: 3046756-3044831 (Counterclockwise)

Preceding gene: 150004905

Following gene: 150004895

Centisome position: 59.01

GC content: 45.17

Gene sequence:

>1926_bases
ATGAAATACGGTTTTGTAAGAGTGGCGGCTGCCATTCCGATAGTGAAAGTGGCTGACTGCAAATTTAATGCACAACAGAT
TGAAACTCAAATTGCCATAGCAGACGGGAAAGGAGTACAAATTATCATTTTCCCCGAACTAAGTATTACAGGGTATACTT
GCGCCGACTTGTTCGGTCAGTCTTTACTGTTGGAAGAAGCAGAAATGGCTTTAATGCAAATTATGAATAATACCCGACAA
ATGGATATCATCTCTATTATAGGGATGCCTGTGGTTATGAATTCCACGCTGCTGAACAGTGCTGTTGTTTTCCAAAAAGG
GAAAATACTGGGTATTGTTCCTAAAACATATTTACCTAATTATAAAGAGTTCTATGAACAACGCTGGTTTACCTCTGCTC
TGAACCACCCGGATGCCAATGTACGCCTCTGCGGACAAAATGTACCGGTCAGCGCCAACCTGCTTTTCGACACTCCGGAA
ACTTGCTTTGGCATTGAAATCTGCGAAGACATGTGGGCCCCCATTCCGCCCAGTTCCGCATTAGCACTGAAAGGAGCGGA
AATCATTTTCAACATGTCTGCCGACAATGAAGGAATAAGCAAGCATAATTATGTCCGTTCATTAGTCAGTCAACAATCAG
CACGCTGTCTGGCAGGATATGTATTCAGTTCATCCGGTTTCGGCGAATCAACCACAGACGTTGTTTTTGCAGGCAACGGA
CTGATATATGAAAACGGCACGTTACTTGCCGAGTCTGAACGTTTCTCATTCAAGGAACAATTGGTCATCAGTGAAATAGA
CGTGGAGCGTCTTCGTGGTGAACGGTTGACCAATACCACTTTTGCCGCCAATATCGGCAACTGCCCGGGACGTCCCGCCA
TCCACATCAGTACAGAATTTGTCAACACACGCGATCTTAGTCTGACGCGCAGTATAGAAGCTCATCCTTTTGTTCCTCAA
GGCAAAGAATTGGATGAACGCTGTGAAGAAATCTTCGCTATCCAAATAGCAGGACTAGCCAAACGTCTGGTACACACTCA
CTGCAAGACTGTAGTAGTCGGTATTTCCGGTGGTCTGGATTCCACCTTGGCACTTTTGGTCTGTGCCAAGACTTTCGATA
AATTGGATCTGCCACGAAAAGGTATCATCGGCATCACCATGCCTGGTTTCGGTACTACCGACCGTACTTATAACAATGCT
CTCCATCTGATGGCTTCACTAGGTGTTACCATTAAAGAAATCAGCATAAAGGAATCCTGCATCCAACACTTTAACGATAT
TAACCACGACATGACCGTACACGATGTAACCTACGAAAACAGCCAGGCACGCGAACGGACTCAAATTCTGATGGATGTTG
CCAACCAATTGGGCGGTCTTGTCATTGGTACGGGAGACCTTTCAGAACTTGCCCTAGGCTGGGCCACCTACAATGGCGAC
CATATGTCTATGTACGGAGTCAATGGAAGCATTCCCAAAACACTGGTCAAATACCTGGTAAATTGGGTGGCTTTGAATGG
CATGGATAATGAATCACGTATTACCCTGCTCGATATTGTAGACACTCCTATCAGTCCCGAACTGATTCCTGCTGATGAAA
ACGGCAATATAAAACAAAAGACAGAAGATTTGGTAGGACCATACGAACTGCATGATTTCTTCCTTTATCAATTCCTACGT
TTCGGTTTTCGTCCCGCCAAGATATTCTTCCTGGCAAGTATCGCTTTTCGTGATACATACGATGAGGAAACAATCAAAAA
ATGGCTTACTATATTCTGCCGCCGTTTCTTTCAACAACAATTCAAACGCTCTTGCCTGCCCGATGGTCCCAAAGTGGGAT
CTGTATCACTCAGTCCCCGAGGTGACTGGCGTATGCCGAGCGATGCCAGTGCAGCTTCATGGCTGAAAGAATGCGAAGAG
CTATAA

Upstream 100 bases:

>100_bases
AAACAAATAAATCACGCACTTTTTTTTATTTTTTTTATTTAGAGGTATAAATCGGGAAAGAATGATTAATTTTGCAGGTA
TATATAATAATAGGTATAAT

Downstream 100 bases:

>100_bases
ACCACATAAAAAAGTAAGTCGGCTTCAAAGACAAAGTAACTTAACTTACTCGCCCAACTAAGTTAAGTTACTTTGTCTTT
GAAGCCGACTTAAATACATC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MKYGFVRVAAAIPIVKVADCKFNAQQIETQIAIADGKGVQIIIFPELSITGYTCADLFGQSLLLEEAEMALMQIMNNTRQ
MDIISIIGMPVVMNSTLLNSAVVFQKGKILGIVPKTYLPNYKEFYEQRWFTSALNHPDANVRLCGQNVPVSANLLFDTPE
TCFGIEICEDMWAPIPPSSALALKGAEIIFNMSADNEGISKHNYVRSLVSQQSARCLAGYVFSSSGFGESTTDVVFAGNG
LIYENGTLLAESERFSFKEQLVISEIDVERLRGERLTNTTFAANIGNCPGRPAIHISTEFVNTRDLSLTRSIEAHPFVPQ
GKELDERCEEIFAIQIAGLAKRLVHTHCKTVVVGISGGLDSTLALLVCAKTFDKLDLPRKGIIGITMPGFGTTDRTYNNA
LHLMASLGVTIKEISIKESCIQHFNDINHDMTVHDVTYENSQARERTQILMDVANQLGGLVIGTGDLSELALGWATYNGD
HMSMYGVNGSIPKTLVKYLVNWVALNGMDNESRITLLDIVDTPISPELIPADENGNIKQKTEDLVGPYELHDFFLYQFLR
FGFRPAKIFFLASIAFRDTYDEETIKKWLTIFCRRFFQQQFKRSCLPDGPKVGSVSLSPRGDWRMPSDASAASWLKECEE
L

Sequences:

>Translated_641_residues
MKYGFVRVAAAIPIVKVADCKFNAQQIETQIAIADGKGVQIIIFPELSITGYTCADLFGQSLLLEEAEMALMQIMNNTRQ
MDIISIIGMPVVMNSTLLNSAVVFQKGKILGIVPKTYLPNYKEFYEQRWFTSALNHPDANVRLCGQNVPVSANLLFDTPE
TCFGIEICEDMWAPIPPSSALALKGAEIIFNMSADNEGISKHNYVRSLVSQQSARCLAGYVFSSSGFGESTTDVVFAGNG
LIYENGTLLAESERFSFKEQLVISEIDVERLRGERLTNTTFAANIGNCPGRPAIHISTEFVNTRDLSLTRSIEAHPFVPQ
GKELDERCEEIFAIQIAGLAKRLVHTHCKTVVVGISGGLDSTLALLVCAKTFDKLDLPRKGIIGITMPGFGTTDRTYNNA
LHLMASLGVTIKEISIKESCIQHFNDINHDMTVHDVTYENSQARERTQILMDVANQLGGLVIGTGDLSELALGWATYNGD
HMSMYGVNGSIPKTLVKYLVNWVALNGMDNESRITLLDIVDTPISPELIPADENGNIKQKTEDLVGPYELHDFFLYQFLR
FGFRPAKIFFLASIAFRDTYDEETIKKWLTIFCRRFFQQQFKRSCLPDGPKVGSVSLSPRGDWRMPSDASAASWLKECEE
L
>Mature_641_residues
MKYGFVRVAAAIPIVKVADCKFNAQQIETQIAIADGKGVQIIIFPELSITGYTCADLFGQSLLLEEAEMALMQIMNNTRQ
MDIISIIGMPVVMNSTLLNSAVVFQKGKILGIVPKTYLPNYKEFYEQRWFTSALNHPDANVRLCGQNVPVSANLLFDTPE
TCFGIEICEDMWAPIPPSSALALKGAEIIFNMSADNEGISKHNYVRSLVSQQSARCLAGYVFSSSGFGESTTDVVFAGNG
LIYENGTLLAESERFSFKEQLVISEIDVERLRGERLTNTTFAANIGNCPGRPAIHISTEFVNTRDLSLTRSIEAHPFVPQ
GKELDERCEEIFAIQIAGLAKRLVHTHCKTVVVGISGGLDSTLALLVCAKTFDKLDLPRKGIIGITMPGFGTTDRTYNNA
LHLMASLGVTIKEISIKESCIQHFNDINHDMTVHDVTYENSQARERTQILMDVANQLGGLVIGTGDLSELALGWATYNGD
HMSMYGVNGSIPKTLVKYLVNWVALNGMDNESRITLLDIVDTPISPELIPADENGNIKQKTEDLVGPYELHDFFLYQFLR
FGFRPAKIFFLASIAFRDTYDEETIKKWLTIFCRRFFQQQFKRSCLPDGPKVGSVSLSPRGDWRMPSDASAASWLKECEE
L

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=354, Percent_Identity=27.683615819209, Blast_Score=120, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17538756, Length=269, Percent_Identity=26.3940520446097, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321865, Length=266, Percent_Identity=29.3233082706767, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24641841, Length=582, Percent_Identity=24.0549828178694, Blast_Score=129, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24641843, Length=582, Percent_Identity=24.0549828178694, Blast_Score=129, Evalue=8e-30,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 71121; Mature: 71121

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50263 CN_HYDROLASE ; PS00176 TOPOISOMERASE_I_EUK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYGFVRVAAAIPIVKVADCKFNAQQIETQIAIADGKGVQIIIFPELSITGYTCADLFGQ
CCCCCEEHHHHCCEEEEECCCCCHHHHEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHH
SLLLEEAEMALMQIMNNTRQMDIISIIGMPVVMNSTLLNSAVVFQKGKILGIVPKTYLPN
HHHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHEHEEECCCEEEEECHHHCCC
YKEFYEQRWFTSALNHPDANVRLCGQNVPVSANLLFDTPETCFGIEICEDMWAPIPPSSA
HHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
LALKGAEIIFNMSADNEGISKHNYVRSLVSQQSARCLAGYVFSSSGFGESTTDVVFAGNG
EEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCE
LIYENGTLLAESERFSFKEQLVISEIDVERLRGERLTNTTFAANIGNCPGRPAIHISTEF
EEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEE
VNTRDLSLTRSIEAHPFVPQGKELDERCEEIFAIQIAGLAKRLVHTHCKTVVVGISGGLD
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCH
STLALLVCAKTFDKLDLPRKGIIGITMPGFGTTDRTYNNALHLMASLGVTIKEISIKESC
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHH
IQHFNDINHDMTVHDVTYENSQARERTQILMDVANQLGGLVIGTGDLSELALGWATYNGD
HHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHEEEEEECCC
HMSMYGVNGSIPKTLVKYLVNWVALNGMDNESRITLLDIVDTPISPELIPADENGNIKQK
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHH
TEDLVGPYELHDFFLYQFLRFGFRPAKIFFLASIAFRDTYDEETIKKWLTIFCRRFFQQQ
HHHCCCCHHHHHHHHHHHHHCCCCHHHEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FKRSCLPDGPKVGSVSLSPRGDWRMPSDASAASWLKECEEL
HHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKYGFVRVAAAIPIVKVADCKFNAQQIETQIAIADGKGVQIIIFPELSITGYTCADLFGQ
CCCCCEEHHHHCCEEEEECCCCCHHHHEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHH
SLLLEEAEMALMQIMNNTRQMDIISIIGMPVVMNSTLLNSAVVFQKGKILGIVPKTYLPN
HHHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHEHEEECCCEEEEECHHHCCC
YKEFYEQRWFTSALNHPDANVRLCGQNVPVSANLLFDTPETCFGIEICEDMWAPIPPSSA
HHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
LALKGAEIIFNMSADNEGISKHNYVRSLVSQQSARCLAGYVFSSSGFGESTTDVVFAGNG
EEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCE
LIYENGTLLAESERFSFKEQLVISEIDVERLRGERLTNTTFAANIGNCPGRPAIHISTEF
EEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEE
VNTRDLSLTRSIEAHPFVPQGKELDERCEEIFAIQIAGLAKRLVHTHCKTVVVGISGGLD
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCH
STLALLVCAKTFDKLDLPRKGIIGITMPGFGTTDRTYNNALHLMASLGVTIKEISIKESC
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHH
IQHFNDINHDMTVHDVTYENSQARERTQILMDVANQLGGLVIGTGDLSELALGWATYNGD
HHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHEEEEEECCC
HMSMYGVNGSIPKTLVKYLVNWVALNGMDNESRITLLDIVDTPISPELIPADENGNIKQK
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHH
TEDLVGPYELHDFFLYQFLRFGFRPAKIFFLASIAFRDTYDEETIKKWLTIFCRRFFQQQ
HHHCCCCHHHHHHHHHHHHHCCCCHHHEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FKRSCLPDGPKVGSVSLSPRGDWRMPSDASAASWLKECEEL
HHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]