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The map label for this gene is lipB [H]

Identifier: 150004778

GI number: 150004778

Start: 2900824

End: 2901600

Strand: Direct

Name: lipB [H]

Synonym: BVU_2240

Alternate gene names: 150004778

Gene position: 2900824-2901600 (Clockwise)

Preceding gene: 150004777

Following gene: 150004780

Centisome position: 56.18

GC content: 46.59

Gene sequence:

>777_bases
ATGAAGATTACAATACTAGGCAGCGGAACGTCTACGGGAGTACCCGAAATAGGGTGCAAATGTCCGGTATGTACCAGCAG
CGATCCCCGGGACAACCGTTTGCGTTGTTCGGGACTGATAGAAACGGAAGGGCTCCGGATTCTGATAGATTGCGGTCCCG
ATTTCCGTGAACAGATGATACGGTTGAACGATTTCAAGCCGTTGGACGGTGTTTTGATTACTCACGAACACTATGACCAT
GTAGGAGGCTTGGATGATTTGCGTCCTTTCTGTCGTTTTCGCGATGTGCCGGTTTATGCCGAACAATATACGGCAGAGCG
TTTACGCCAGCGTATTCCTTATTGTTTTGCCGAACATCTTTATCCGGGAGTTCCCCGTATTACATTAGAGGAAATTGTTC
CCGATTCTCCTTTTATTATCGGCAATCCCGAAGGAAACAAAGTAGAGGTGACACCTCTTCGGGTAATGCATGGCAAACTG
TCCATTTTGGGATACCGTATCGGCCGGATGGCATGGATTACCGATATGTTGACGATGCCTGGGGCTGAATATGATTTGCT
GCAAGGACTGGATGTGTTGGTAATGAATGCATTGCGTCCTCAGCCTCACCATACCCACCAGAATTTGAAGGAGGCTTTGG
ACAATGCACAAAGAATAGGAGCCGGGGAAACCTATTTTATTCACATGAGTCATCATATAGGATTACATGCTGATGCTGAA
CGGTTATTGCCGCCGCATGTGCATTTTGCATACGATGGAATGAAAATTTGTTTATAA

Upstream 100 bases:

>100_bases
GAGATGAAGTGATTGCTCTTGCCAGACGGATACAGGAGTCTGTGCTTCAAAAGTTCGGGGTAGCTATCTCTCCCGAAGTG
AATTTTATATAAAGTATAAT

Downstream 100 bases:

>100_bases
AGCCGGCTTCAACGAATACCGGCACAATCCGTTCAACCCGCATTCCGAACATTTCGGTGTCCGTGCCACACAAACATATC
TTCCGTGTAAAATAAGCCAA

Product: putative hydrolase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKITILGSGTSTGVPEIGCKCPVCTSSDPRDNRLRCSGLIETEGLRILIDCGPDFREQMIRLNDFKPLDGVLITHEHYDH
VGGLDDLRPFCRFRDVPVYAEQYTAERLRQRIPYCFAEHLYPGVPRITLEEIVPDSPFIIGNPEGNKVEVTPLRVMHGKL
SILGYRIGRMAWITDMLTMPGAEYDLLQGLDVLVMNALRPQPHHTHQNLKEALDNAQRIGAGETYFIHMSHHIGLHADAE
RLLPPHVHFAYDGMKICL

Sequences:

>Translated_258_residues
MKITILGSGTSTGVPEIGCKCPVCTSSDPRDNRLRCSGLIETEGLRILIDCGPDFREQMIRLNDFKPLDGVLITHEHYDH
VGGLDDLRPFCRFRDVPVYAEQYTAERLRQRIPYCFAEHLYPGVPRITLEEIVPDSPFIIGNPEGNKVEVTPLRVMHGKL
SILGYRIGRMAWITDMLTMPGAEYDLLQGLDVLVMNALRPQPHHTHQNLKEALDNAQRIGAGETYFIHMSHHIGLHADAE
RLLPPHVHFAYDGMKICL
>Mature_258_residues
MKITILGSGTSTGVPEIGCKCPVCTSSDPRDNRLRCSGLIETEGLRILIDCGPDFREQMIRLNDFKPLDGVLITHEHYDH
VGGLDDLRPFCRFRDVPVYAEQYTAERLRQRIPYCFAEHLYPGVPRITLEEIVPDSPFIIGNPEGNKVEVTPLRVMHGKL
SILGYRIGRMAWITDMLTMPGAEYDLLQGLDVLVMNALRPQPHHTHQNLKEALDNAQRIGAGETYFIHMSHHIGLHADAE
RLLPPHVHFAYDGMKICL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=260, Percent_Identity=28.0769230769231, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 29143; Mature: 29143

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITILGSGTSTGVPEIGCKCPVCTSSDPRDNRLRCSGLIETEGLRILIDCGPDFREQMI
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHH
RLNDFKPLDGVLITHEHYDHVGGLDDLRPFCRFRDVPVYAEQYTAERLRQRIPYCFAEHL
HHCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHH
YPGVPRITLEEIVPDSPFIIGNPEGNKVEVTPLRVMHGKLSILGYRIGRMAWITDMLTMP
CCCCCCEEHHHHCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
GAEYDLLQGLDVLVMNALRPQPHHTHQNLKEALDNAQRIGAGETYFIHMSHHIGLHADAE
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHH
RLLPPHVHFAYDGMKICL
HCCCCCEEEEECCEEECC
>Mature Secondary Structure
MKITILGSGTSTGVPEIGCKCPVCTSSDPRDNRLRCSGLIETEGLRILIDCGPDFREQMI
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHH
RLNDFKPLDGVLITHEHYDHVGGLDDLRPFCRFRDVPVYAEQYTAERLRQRIPYCFAEHL
HHCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHH
YPGVPRITLEEIVPDSPFIIGNPEGNKVEVTPLRVMHGKLSILGYRIGRMAWITDMLTMP
CCCCCCEEHHHHCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
GAEYDLLQGLDVLVMNALRPQPHHTHQNLKEALDNAQRIGAGETYFIHMSHHIGLHADAE
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHH
RLLPPHVHFAYDGMKICL
HCCCCCEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]