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The map label for this gene is pheS

Identifier: 150004775

GI number: 150004775

Start: 2897784

End: 2898818

Strand: Reverse

Name: pheS

Synonym: BVU_2237

Alternate gene names: 150004775

Gene position: 2898818-2897784 (Counterclockwise)

Preceding gene: 150004779

Following gene: 150004774

Centisome position: 56.14

GC content: 45.22

Gene sequence:

>1035_bases
ATGTTAGCTAAAATAGAACAATTACTTCAGGAAATTGAAGGCTTGCAAGCAGCCAATGCGGAAGAATTGGAAGCCTTGCG
CATTAAATATCTTAGCAAAAAAGGAGCGATTAATGAGTTGATGGCAGACTTCCGCAACGTACCTGCCGAGCAGAAAAAAG
AGGTGGGTATGAAACTGAACGAGCTGAAGAACAAGGCTCAGGAGCGTATCGCCTCATTGAAAGAAGCTTTCGAAACCCAA
GATAACAGCGCAGCCGAAATGGATCTTACCCGTACGGCTTATCCCATCGAACTGGGAACACGTCACCCGCTGAGCATTGT
AAAGAATGAAATCATTGATATATTCCATCGGCTGGGCTTCAGTATTGCCGACGGTCCCGAAATAGAAGACGACTTGCACG
TATTCACTGCCATGAATTTTGCCGAAGACCATCCTGCACGTGATATGCAAGATACTTTCTTCATCGAAGCCAATCAGGAA
GATGTGAAAAAGAACATCGTACTTCGCACTCACACCTCTTCCGTACAGGCAAGAGCTATGGAACATTCGCAACCTCCTAT
CCGCATCATCTGTCCGGGACGTGTATACCGCAATGAAGCGATCAGTTATCGTGCCCATGCATTCTTCCATCAAGTGGAAG
CACTCTACATTGACAAGAATGTATCATTCACTGATTTGAAGCAGGTATTGCTGTTGTTTGCCAAAGAAATGTTCGGCGAA
GATACTCAAATCCGTCTGCGTCCGTCCTATTTCCCGTTCACCGAACCAAGTGCCGAAATGGATATCAGTTGTAATATTTG
CGGCGGAAAAGGTTGTCCGTTCTGTAAAGGCACAGGCTGGGTGGAAATTCTGGGATGTGGTATGGTGGATCCGAATGTAT
TGGAAGCCAACGGCATTGACAGCAAAGTTTACTCAGGCTATGCGCTAGGTATGGGTATTGAACGCATTACCAACTTGAAA
TATCGTGTAAACGACTTACGATTATTCTCTGAAAACGATACCCGCTTCCTGAAAGAATTCGAAGCAGCCAATTAA

Upstream 100 bases:

>100_bases
AGAAAAAATCGATGCAAAAATATCGTTTTTTTTAGTTTTCAGCAAAATATATGGGTTTAATTCGCTAACTTTGCGGTCAT
TAAAAAATAAAGAAGAAACT

Downstream 100 bases:

>100_bases
TAATGTGTCAATGTGACCATGTGCTGATATGCCAATTGCCATGCGGAATAGAACGCGGTCAATTGGCATATTAACATATT
GCCACATTAGCATATTGATA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLAKIEQLLQEIEGLQAANAEELEALRIKYLSKKGAINELMADFRNVPAEQKKEVGMKLNELKNKAQERIASLKEAFETQ
DNSAAEMDLTRTAYPIELGTRHPLSIVKNEIIDIFHRLGFSIADGPEIEDDLHVFTAMNFAEDHPARDMQDTFFIEANQE
DVKKNIVLRTHTSSVQARAMEHSQPPIRIICPGRVYRNEAISYRAHAFFHQVEALYIDKNVSFTDLKQVLLLFAKEMFGE
DTQIRLRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLEANGIDSKVYSGYALGMGIERITNLK
YRVNDLRLFSENDTRFLKEFEAAN

Sequences:

>Translated_344_residues
MLAKIEQLLQEIEGLQAANAEELEALRIKYLSKKGAINELMADFRNVPAEQKKEVGMKLNELKNKAQERIASLKEAFETQ
DNSAAEMDLTRTAYPIELGTRHPLSIVKNEIIDIFHRLGFSIADGPEIEDDLHVFTAMNFAEDHPARDMQDTFFIEANQE
DVKKNIVLRTHTSSVQARAMEHSQPPIRIICPGRVYRNEAISYRAHAFFHQVEALYIDKNVSFTDLKQVLLLFAKEMFGE
DTQIRLRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLEANGIDSKVYSGYALGMGIERITNLK
YRVNDLRLFSENDTRFLKEFEAAN
>Mature_344_residues
MLAKIEQLLQEIEGLQAANAEELEALRIKYLSKKGAINELMADFRNVPAEQKKEVGMKLNELKNKAQERIASLKEAFETQ
DNSAAEMDLTRTAYPIELGTRHPLSIVKNEIIDIFHRLGFSIADGPEIEDDLHVFTAMNFAEDHPARDMQDTFFIEANQE
DVKKNIVLRTHTSSVQARAMEHSQPPIRIICPGRVYRNEAISYRAHAFFHQVEALYIDKNVSFTDLKQVLLLFAKEMFGE
DTQIRLRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLEANGIDSKVYSGYALGMGIERITNLK
YRVNDLRLFSENDTRFLKEFEAAN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=300, Percent_Identity=27.6666666666667, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4758340, Length=268, Percent_Identity=31.3432835820896, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1788007, Length=330, Percent_Identity=46.0606060606061, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=31.9702602230483, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=31.9702602230483, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566635, Length=292, Percent_Identity=28.7671232876712, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=269, Percent_Identity=31.2267657992565, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI18858079, Length=276, Percent_Identity=28.9855072463768, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137424, Length=301, Percent_Identity=26.2458471760797, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BACV8 (A6L2I3)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299519.1
- ProteinModelPortal:   A6L2I3
- SMR:   A6L2I3
- STRING:   A6L2I3
- GeneID:   5303201
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2237
- NMPDR:   fig|435590.6.peg.2096
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38980; Mature: 38980

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKIEQLLQEIEGLQAANAEELEALRIKYLSKKGAINELMADFRNVPAEQKKEVGMKLN
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCHHH
ELKNKAQERIASLKEAFETQDNSAAEMDLTRTAYPIELGTRHPLSIVKNEIIDIFHRLGF
HHHHHHHHHHHHHHHHHHCCCCCCHHEEHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCC
SIADGPEIEDDLHVFTAMNFAEDHPARDMQDTFFIEANQEDVKKNIVLRTHTSSVQARAM
CCCCCCCCCHHHHHHHHHCHHHCCCCCCCCCEEEEECCHHHHHHCEEEEECCCHHHHHHH
EHSQPPIRIICPGRVYRNEAISYRAHAFFHQVEALYIDKNVSFTDLKQVLLLFAKEMFGE
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCC
DTQIRLRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLEANGID
CCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEECCCCC
SKVYSGYALGMGIERITNLKYRVNDLRLFSENDTRFLKEFEAAN
CHHHCCCHHHCCHHHHHCCCEEEEEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MLAKIEQLLQEIEGLQAANAEELEALRIKYLSKKGAINELMADFRNVPAEQKKEVGMKLN
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCHHH
ELKNKAQERIASLKEAFETQDNSAAEMDLTRTAYPIELGTRHPLSIVKNEIIDIFHRLGF
HHHHHHHHHHHHHHHHHHCCCCCCHHEEHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCC
SIADGPEIEDDLHVFTAMNFAEDHPARDMQDTFFIEANQEDVKKNIVLRTHTSSVQARAM
CCCCCCCCCHHHHHHHHHCHHHCCCCCCCCCEEEEECCHHHHHHCEEEEECCCHHHHHHH
EHSQPPIRIICPGRVYRNEAISYRAHAFFHQVEALYIDKNVSFTDLKQVLLLFAKEMFGE
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCC
DTQIRLRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLEANGID
CCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEECCCCC
SKVYSGYALGMGIERITNLKYRVNDLRLFSENDTRFLKEFEAAN
CHHHCCCHHHCCHHHHHCCCEEEEEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA