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The map label for this gene is lacZ [H]

Identifier: 150004762

GI number: 150004762

Start: 2874576

End: 2876390

Strand: Direct

Name: lacZ [H]

Synonym: BVU_2224

Alternate gene names: 150004762

Gene position: 2874576-2876390 (Clockwise)

Preceding gene: 150004761

Following gene: 150004763

Centisome position: 55.67

GC content: 40.99

Gene sequence:

>1815_bases
ATGAAGAATTTTCTTATCTTATCTCTGTTGTTAGCCTTTTTGACTACGGAAGTATGTGCGCAGTGGAAACCGGCTGGTGA
CAAGATCAAAACACAGTGGGCAAATAAAATAAATACCTCTGCCGTATTGCCTGAATATCCACGTCCTATAATGGAACGTA
ATGAATGGAAGAACTTGAATGGTTTGTGGGAGTATGCTATCACCGATTTGGGAGGAAATGTTCCGGCGCATTTTGACGGG
CAGATTTTGGTACCTTTTGCAGTGGAATCTTCTTTATCAGGTGTGGGACAAAGAGTCGGTGCAAAAAAGGAATTATGGTA
TTCCCGTTCATTTGAAGTGCCTGTTGCTTGGAAAAATAAAAATGTATTACTTCATTTCGGTGCTGTGGACTGGAAAGCAG
ATATTTGGGTAAATGATGTGAAGGTTGGTAGTCATACTGGTGGTTTTACTCCGTTTTCTTTTGATATCACTGCAGCGTTG
TCTGGTAAGGGAAATAATAAATTAGTAGTAAAAGTTTGGGACCCTACGGATGAAGGGTATCAGCCTAGAGGTAAACAGGT
GAATAATCCGGAGGGTATTTGGTATACTCCTGTGACTGGCATTTGGCAGACTGTTTGGCTGGAACCGGTTACTGCCAGAC
ATATTGAAGAACTTCGAATCACACCGGATATTGACCAACACTTACTGACTGTAAAAGCCCATTTGAATATGAGGGCACCT
TCCGATCTGATTGAAGTAAACGTATATGACGGCAATCAGTTGGTTGCCACTGGGAAAAGTATTAATGATGAGGCTGTGGT
CATACCTATGCCGGAAGATGCTAAATTATGGAGTCCTGATTTTCCCTTTTTGTATACTTTAAAGGTTATGTTGAAAAGCG
ATAATAAAATATTGGATCAGGTGAACAGTTATGCAGCTATGCGCAAATACTCTACGAAACGTGATGCTAATGGAATTGTA
CGTTTAGAACTGAATAACCAACCTTTGTTCCAGTTCGGACCATTGGATCAGGGATGGTGGCCTGATGGATTGTATACCGC
TCCTACTGATGAGGCTTTAGTATATGATATACAAAAGACTAAATACTTAGGATTCAATATGATACGTAAACATATTAAAG
TGGAACCGGCCCGTTGGTATACTCATTGTGATCAATTGGGAATTATTGTGTGGCAGGATATGCCTAGCGGTGATCGTAAT
CCGGAGTGGCAGAACCGCAAATATTTTGAAGGTACGGAAAAAAAGCGTTCGGCGGTTTCTGAGGCTTGTTACCGTAAAGA
ATGGAAAGAAATTATGGATTGCTTGTATTCTTATCCATGTATCGGAACATGGGTTCCGTTCAATGAGGCATGGGGGCAAT
TCAAGACTCCGGAAATTGCAGAATGGACTAAAGAATATGATCCGACCCGTCTGGTAAATCCTGCAAGTGGAGGTAACCAT
TATACTTGTGGCGATATGCTTGACTTGCATAATTATCCTGCTCCCGAAATGTATCTGTATGATGCCCAGCGAGCTAATGT
TTTGGGAGAGTATGGTGGTATCGGTTGGGTAGTGAAAAATCATATTTGGGAACCGGATCGTAACTGGGGATATATACAGT
TTAATTCTTCCAAAGAAGTAACTGATGAGTATATAAAATATACAGATATGCTTTATGACCTGATAATCAGGGGATTCTCG
GCGGCTGTTTATACTCAGACTACGGATGTGGAGGTGGAAGTGAACGGACTGATGACTTATGATCGTAAGGTGATTAAAGT
AGATGAAAAACGAGTGAGAGAAGCCAACGCACGTATCTGTAAATCATTAAAGTAA

Upstream 100 bases:

>100_bases
TTTGAGCGGATTATTTTAATTATTGGAAGAATTGTTCTATCCTTTAAAGCATATTGCTTCTATCTTTGCCATACTGAGAA
AAACATTAAATTACACTATT

Downstream 100 bases:

>100_bases
GGAATATGAATAAGCTGTTCTTTTGGGTATGTGCAGTCCTTATGACTGCATGTGCTTCTTATAAAAATGATGAGGTCTTG
ACTGAATCCGGTTTAAGTAA

Product: beta-glycosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MKNFLILSLLLAFLTTEVCAQWKPAGDKIKTQWANKINTSAVLPEYPRPIMERNEWKNLNGLWEYAITDLGGNVPAHFDG
QILVPFAVESSLSGVGQRVGAKKELWYSRSFEVPVAWKNKNVLLHFGAVDWKADIWVNDVKVGSHTGGFTPFSFDITAAL
SGKGNNKLVVKVWDPTDEGYQPRGKQVNNPEGIWYTPVTGIWQTVWLEPVTARHIEELRITPDIDQHLLTVKAHLNMRAP
SDLIEVNVYDGNQLVATGKSINDEAVVIPMPEDAKLWSPDFPFLYTLKVMLKSDNKILDQVNSYAAMRKYSTKRDANGIV
RLELNNQPLFQFGPLDQGWWPDGLYTAPTDEALVYDIQKTKYLGFNMIRKHIKVEPARWYTHCDQLGIIVWQDMPSGDRN
PEWQNRKYFEGTEKKRSAVSEACYRKEWKEIMDCLYSYPCIGTWVPFNEAWGQFKTPEIAEWTKEYDPTRLVNPASGGNH
YTCGDMLDLHNYPAPEMYLYDAQRANVLGEYGGIGWVVKNHIWEPDRNWGYIQFNSSKEVTDEYIKYTDMLYDLIIRGFS
AAVYTQTTDVEVEVNGLMTYDRKVIKVDEKRVREANARICKSLK

Sequences:

>Translated_604_residues
MKNFLILSLLLAFLTTEVCAQWKPAGDKIKTQWANKINTSAVLPEYPRPIMERNEWKNLNGLWEYAITDLGGNVPAHFDG
QILVPFAVESSLSGVGQRVGAKKELWYSRSFEVPVAWKNKNVLLHFGAVDWKADIWVNDVKVGSHTGGFTPFSFDITAAL
SGKGNNKLVVKVWDPTDEGYQPRGKQVNNPEGIWYTPVTGIWQTVWLEPVTARHIEELRITPDIDQHLLTVKAHLNMRAP
SDLIEVNVYDGNQLVATGKSINDEAVVIPMPEDAKLWSPDFPFLYTLKVMLKSDNKILDQVNSYAAMRKYSTKRDANGIV
RLELNNQPLFQFGPLDQGWWPDGLYTAPTDEALVYDIQKTKYLGFNMIRKHIKVEPARWYTHCDQLGIIVWQDMPSGDRN
PEWQNRKYFEGTEKKRSAVSEACYRKEWKEIMDCLYSYPCIGTWVPFNEAWGQFKTPEIAEWTKEYDPTRLVNPASGGNH
YTCGDMLDLHNYPAPEMYLYDAQRANVLGEYGGIGWVVKNHIWEPDRNWGYIQFNSSKEVTDEYIKYTDMLYDLIIRGFS
AAVYTQTTDVEVEVNGLMTYDRKVIKVDEKRVREANARICKSLK
>Mature_604_residues
MKNFLILSLLLAFLTTEVCAQWKPAGDKIKTQWANKINTSAVLPEYPRPIMERNEWKNLNGLWEYAITDLGGNVPAHFDG
QILVPFAVESSLSGVGQRVGAKKELWYSRSFEVPVAWKNKNVLLHFGAVDWKADIWVNDVKVGSHTGGFTPFSFDITAAL
SGKGNNKLVVKVWDPTDEGYQPRGKQVNNPEGIWYTPVTGIWQTVWLEPVTARHIEELRITPDIDQHLLTVKAHLNMRAP
SDLIEVNVYDGNQLVATGKSINDEAVVIPMPEDAKLWSPDFPFLYTLKVMLKSDNKILDQVNSYAAMRKYSTKRDANGIV
RLELNNQPLFQFGPLDQGWWPDGLYTAPTDEALVYDIQKTKYLGFNMIRKHIKVEPARWYTHCDQLGIIVWQDMPSGDRN
PEWQNRKYFEGTEKKRSAVSEACYRKEWKEIMDCLYSYPCIGTWVPFNEAWGQFKTPEIAEWTKEYDPTRLVNPASGGNH
YTCGDMLDLHNYPAPEMYLYDAQRANVLGEYGGIGWVVKNHIWEPDRNWGYIQFNSSKEVTDEYIKYTDMLYDLIIRGFS
AAVYTQTTDVEVEVNGLMTYDRKVIKVDEKRVREANARICKSLK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787903, Length=451, Percent_Identity=21.7294900221729, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17510825, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24655438, Length=512, Percent_Identity=21.09375, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62471735, Length=475, Percent_Identity=21.6842105263158, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922582, Length=475, Percent_Identity=21.6842105263158, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 69357; Mature: 69357

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFLILSLLLAFLTTEVCAQWKPAGDKIKTQWANKINTSAVLPEYPRPIMERNEWKNLN
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCC
GLWEYAITDLGGNVPAHFDGQILVPFAVESSLSGVGQRVGAKKELWYSRSFEVPVAWKNK
HHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCCEEEEECCC
NVLLHFGAVDWKADIWVNDVKVGSHTGGFTPFSFDITAALSGKGNNKLVVKVWDPTDEGY
CEEEEECCCCEEEEEEEEEEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCC
QPRGKQVNNPEGIWYTPVTGIWQTVWLEPVTARHIEELRITPDIDQHLLTVKAHLNMRAP
CCCCCCCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHEECCCCCCHHEEEEEEEECCCCC
SDLIEVNVYDGNQLVATGKSINDEAVVIPMPEDAKLWSPDFPFLYTLKVMLKSDNKILDQ
CCEEEEEEECCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHEEEEHHCCCCHHHHH
VNSYAAMRKYSTKRDANGIVRLELNNQPLFQFGPLDQGWWPDGLYTAPTDEALVYDIQKT
HHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCEEEEEEHHH
KYLGFNMIRKHIKVEPARWYTHCDQLGIIVWQDMPSGDRNPEWQNRKYFEGTEKKRSAVS
HHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
EACYRKEWKEIMDCLYSYPCIGTWVPFNEAWGQFKTPEIAEWTKEYDPTRLVNPASGGNH
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCHHHHHHCCCCCEEECCCCCCCE
YTCGDMLDLHNYPAPEMYLYDAQRANVLGEYGGIGWVVKNHIWEPDRNWGYIQFNSSKEV
EECCCHHHHCCCCCCCEEEEECHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCHH
TDEYIKYTDMLYDLIIRGFSAAVYTQTTDVEVEVNGLMTYDRKVIKVDEKRVREANARIC
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCEEEECCEEEEECHHHHHHHHHHHH
KSLK
HCCC
>Mature Secondary Structure
MKNFLILSLLLAFLTTEVCAQWKPAGDKIKTQWANKINTSAVLPEYPRPIMERNEWKNLN
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCC
GLWEYAITDLGGNVPAHFDGQILVPFAVESSLSGVGQRVGAKKELWYSRSFEVPVAWKNK
HHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCCEEEEECCC
NVLLHFGAVDWKADIWVNDVKVGSHTGGFTPFSFDITAALSGKGNNKLVVKVWDPTDEGY
CEEEEECCCCEEEEEEEEEEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCC
QPRGKQVNNPEGIWYTPVTGIWQTVWLEPVTARHIEELRITPDIDQHLLTVKAHLNMRAP
CCCCCCCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHEECCCCCCHHEEEEEEEECCCCC
SDLIEVNVYDGNQLVATGKSINDEAVVIPMPEDAKLWSPDFPFLYTLKVMLKSDNKILDQ
CCEEEEEEECCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHEEEEHHCCCCHHHHH
VNSYAAMRKYSTKRDANGIVRLELNNQPLFQFGPLDQGWWPDGLYTAPTDEALVYDIQKT
HHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCEEEEEEHHH
KYLGFNMIRKHIKVEPARWYTHCDQLGIIVWQDMPSGDRNPEWQNRKYFEGTEKKRSAVS
HHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
EACYRKEWKEIMDCLYSYPCIGTWVPFNEAWGQFKTPEIAEWTKEYDPTRLVNPASGGNH
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCHHHHHHCCCCCEEECCCCCCCE
YTCGDMLDLHNYPAPEMYLYDAQRANVLGEYGGIGWVVKNHIWEPDRNWGYIQFNSSKEV
EECCCHHHHCCCCCCCEEEEECHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCHH
TDEYIKYTDMLYDLIIRGFSAAVYTQTTDVEVEVNGLMTYDRKVIKVDEKRVREANARIC
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCEEEECCEEEEECHHHHHHHHHHHH
KSLK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA