Click here to switch to the map view.

The map label for this gene is rhgT [H]

Identifier: 150004699

GI number: 150004699

Start: 2779745

End: 2781445

Strand: Direct

Name: rhgT [H]

Synonym: BVU_2160

Alternate gene names: 150004699

Gene position: 2779745-2781445 (Clockwise)

Preceding gene: 150004698

Following gene: 150004700

Centisome position: 53.84

GC content: 40.92

Gene sequence:

>1701_bases
ATGAGAAAGTTGAATTCGAAATTACTATATGTATTTTGTGCGCTTCTTTTCTTTTCCGCTTTTACTAGCGGAAAGAAGGA
TATAATCACCATTTTTATGATAGGTGATTCTACTATGGCGAATAAAAGTCTGAAGAATGGAAATATTGAACGTGGTTGGG
GACAGATGCTTCCTGAATTTTTGATTGGCGATGTACGGGTGGACAATCATGCCATGAATGGTCGGAGTTCACAGAGTTTT
ATCGATGAAGGACGTTGGGATGTCGTACTCTCCAAATTGAAGAGCGGAGACTATGTATTTATCCAGTTCGGTCATAATGA
TGAGAAATCTGCCGAAGAGCTACATACTATTCCCGGAAGCACTTTCGATGAAAACTTGCGTAGATTTGTAAGGGAGACGC
GAGCAAAGGGAGCTTATCCGGTGCTGTTTAATTCAATTGTGCGCCGGAATTTTCCACCGGAAGGGACCATTGGGCATAAA
GGCAGTTATGAGACGGAGGGAAGCACATTGGTAGACACTCATGGAGAGTATTTGGAATCACCAAGAAGAGTGGCAAAAGA
AATGGATGTTCCTTTTGTAGATTTGAATAAGCTGACGTATGATTTGGTAGTCAGTATGGGGGTAGAGAAGTCAAAAAGCC
TTTTCATGTGGGTACCTACGGGAATATATGATTTCTGCCCAAAAGGAAAAATTGATAACACCCATCTGAACATTTCTGGT
GGAAAGGTGGTGGCGGGAATCGCGATGGAAGCTGTTGCCAAAGTTGTGCCGGAACTAGCTGCATATATGCGTTCTCACGA
TTCGGAAGTATATGTGGCAGATTATAAGGACGATAAGCAATGTGCTATCAGCTATACTTTCGATGATGGTCTGGAAGAAC
ACTATACGCTTGTTTTCCCGGAAATGGAAAAAGTAGGTTTCAAAGGGACTTTTTGGATATGGGGAAAAGGGATAGAGAAT
GTGACGGAGCAGCAAGATAAACCACGTATGACTTGGACGCAAATGAAAGAAATGTTTGATAAAGGGCATGAAATATCCAG
TCACGGTTGGTCACATGCAGATTTGAGGCATCTTAGTCTGGAAGAAGTGAAAGCGGAAGTTGGCAGGAATGATAGCATTA
TTCAGGCTGAAATAGGCGAAAGGCCGCTAACCTTCTGTTATCCGTTTAATTCTTATAATGAAGATGTACGGCGGATTGTT
TCAGAAAATAGAATAGGTACACGTATCAAACAATATGCCATAGGAGGTGAGAAATCAAAATCAACGGTTGAATCGCTGGA
TAAATGGGTAAAGGAGCTCATGATTTCCAATGATTGGGGAGTGACTATGATACATGGAATTTCTACCGGTTATGATGCCT
TCACTTCCCCGGATATATTATGGGAACATTTTCGTAGAGTGAAAAATCAGGAATACGATATTTGGGTAGGTACTTTTCGC
GAGGTAGCGGCATATGTTAAAGAGCGGAGGAATGTACAACTGGATATTGTGAAAAAGGAATCGCAGTGGGCAGTTATACC
TCGTTTGTTACTGGACAAGGAATTATTTAATGAACCTTTGACAATGGTGCTGAATAAGAAAGGAAAGGGAAAAGTGAAGG
TGTATCAAAACGGAAAGAGACTGTTAGTAAAAAAACGGGATGATAAACAGGTCTTTGATTTCAATCCGTATGGGGGAACT
ATAAGAATAAGTTTTTTTTGA

Upstream 100 bases:

>100_bases
AGAGATTGGTAGTATTATCATTATGCTATTGCCGTATGAACTGTAATTGTACATTATCATATATCTTATTTTTATGTTCT
TTCTTGTTAAAAAATATATC

Downstream 100 bases:

>100_bases
TAATCCAGCACAGCTAAAGGAACATCCCGTTGGTGGAATTTATCTCGAACTTGATTCTGCCAATAGGTAGGAGAAAATAT
CGTTTATAAGAGCAGATTAG

Product: carbohydrate esterase family 12 protein

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MRKLNSKLLYVFCALLFFSAFTSGKKDIITIFMIGDSTMANKSLKNGNIERGWGQMLPEFLIGDVRVDNHAMNGRSSQSF
IDEGRWDVVLSKLKSGDYVFIQFGHNDEKSAEELHTIPGSTFDENLRRFVRETRAKGAYPVLFNSIVRRNFPPEGTIGHK
GSYETEGSTLVDTHGEYLESPRRVAKEMDVPFVDLNKLTYDLVVSMGVEKSKSLFMWVPTGIYDFCPKGKIDNTHLNISG
GKVVAGIAMEAVAKVVPELAAYMRSHDSEVYVADYKDDKQCAISYTFDDGLEEHYTLVFPEMEKVGFKGTFWIWGKGIEN
VTEQQDKPRMTWTQMKEMFDKGHEISSHGWSHADLRHLSLEEVKAEVGRNDSIIQAEIGERPLTFCYPFNSYNEDVRRIV
SENRIGTRIKQYAIGGEKSKSTVESLDKWVKELMISNDWGVTMIHGISTGYDAFTSPDILWEHFRRVKNQEYDIWVGTFR
EVAAYVKERRNVQLDIVKKESQWAVIPRLLLDKELFNEPLTMVLNKKGKGKVKVYQNGKRLLVKKRDDKQVFDFNPYGGT
IRISFF

Sequences:

>Translated_566_residues
MRKLNSKLLYVFCALLFFSAFTSGKKDIITIFMIGDSTMANKSLKNGNIERGWGQMLPEFLIGDVRVDNHAMNGRSSQSF
IDEGRWDVVLSKLKSGDYVFIQFGHNDEKSAEELHTIPGSTFDENLRRFVRETRAKGAYPVLFNSIVRRNFPPEGTIGHK
GSYETEGSTLVDTHGEYLESPRRVAKEMDVPFVDLNKLTYDLVVSMGVEKSKSLFMWVPTGIYDFCPKGKIDNTHLNISG
GKVVAGIAMEAVAKVVPELAAYMRSHDSEVYVADYKDDKQCAISYTFDDGLEEHYTLVFPEMEKVGFKGTFWIWGKGIEN
VTEQQDKPRMTWTQMKEMFDKGHEISSHGWSHADLRHLSLEEVKAEVGRNDSIIQAEIGERPLTFCYPFNSYNEDVRRIV
SENRIGTRIKQYAIGGEKSKSTVESLDKWVKELMISNDWGVTMIHGISTGYDAFTSPDILWEHFRRVKNQEYDIWVGTFR
EVAAYVKERRNVQLDIVKKESQWAVIPRLLLDKELFNEPLTMVLNKKGKGKVKVYQNGKRLLVKKRDDKQVFDFNPYGGT
IRISFF
>Mature_566_residues
MRKLNSKLLYVFCALLFFSAFTSGKKDIITIFMIGDSTMANKSLKNGNIERGWGQMLPEFLIGDVRVDNHAMNGRSSQSF
IDEGRWDVVLSKLKSGDYVFIQFGHNDEKSAEELHTIPGSTFDENLRRFVRETRAKGAYPVLFNSIVRRNFPPEGTIGHK
GSYETEGSTLVDTHGEYLESPRRVAKEMDVPFVDLNKLTYDLVVSMGVEKSKSLFMWVPTGIYDFCPKGKIDNTHLNISG
GKVVAGIAMEAVAKVVPELAAYMRSHDSEVYVADYKDDKQCAISYTFDDGLEEHYTLVFPEMEKVGFKGTFWIWGKGIEN
VTEQQDKPRMTWTQMKEMFDKGHEISSHGWSHADLRHLSLEEVKAEVGRNDSIIQAEIGERPLTFCYPFNSYNEDVRRIV
SENRIGTRIKQYAIGGEKSKSTVESLDKWVKELMISNDWGVTMIHGISTGYDAFTSPDILWEHFRRVKNQEYDIWVGTFR
EVAAYVKERRNVQLDIVKKESQWAVIPRLLLDKELFNEPLTMVLNKKGKGKVKVYQNGKRLLVKKRDDKQVFDFNPYGGT
IRISFF

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 64888; Mature: 64888

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLNSKLLYVFCALLFFSAFTSGKKDIITIFMIGDSTMANKSLKNGNIERGWGQMLPEF
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHH
LIGDVRVDNHAMNGRSSQSFIDEGRWDVVLSKLKSGDYVFIQFGHNDEKSAEELHTIPGS
HHCCEEECCCCCCCCCCHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCC
TFDENLRRFVRETRAKGAYPVLFNSIVRRNFPPEGTIGHKGSYETEGSTLVDTHGEYLES
CHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHH
PRRVAKEMDVPFVDLNKLTYDLVVSMGVEKSKSLFMWVPTGIYDFCPKGKIDNTHLNISG
HHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCCCCCEEEECC
GKVVAGIAMEAVAKVVPELAAYMRSHDSEVYVADYKDDKQCAISYTFDDGLEEHYTLVFP
CCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEEC
EMEKVGFKGTFWIWGKGIENVTEQQDKPRMTWTQMKEMFDKGHEISSHGWSHADLRHLSL
CHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EEVKAEVGRNDSIIQAEIGERPLTFCYPFNSYNEDVRRIVSENRIGTRIKQYAIGGEKSK
HHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCH
STVESLDKWVKELMISNDWGVTMIHGISTGYDAFTSPDILWEHFRRVKNQEYDIWVGTFR
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHH
EVAAYVKERRNVQLDIVKKESQWAVIPRLLLDKELFNEPLTMVLNKKGKGKVKVYQNGKR
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCE
LLVKKRDDKQVFDFNPYGGTIRISFF
EEEEECCCCEEEEECCCCCEEEEEEC
>Mature Secondary Structure
MRKLNSKLLYVFCALLFFSAFTSGKKDIITIFMIGDSTMANKSLKNGNIERGWGQMLPEF
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHH
LIGDVRVDNHAMNGRSSQSFIDEGRWDVVLSKLKSGDYVFIQFGHNDEKSAEELHTIPGS
HHCCEEECCCCCCCCCCHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCC
TFDENLRRFVRETRAKGAYPVLFNSIVRRNFPPEGTIGHKGSYETEGSTLVDTHGEYLES
CHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHH
PRRVAKEMDVPFVDLNKLTYDLVVSMGVEKSKSLFMWVPTGIYDFCPKGKIDNTHLNISG
HHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCCCCCEEEECC
GKVVAGIAMEAVAKVVPELAAYMRSHDSEVYVADYKDDKQCAISYTFDDGLEEHYTLVFP
CCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEEC
EMEKVGFKGTFWIWGKGIENVTEQQDKPRMTWTQMKEMFDKGHEISSHGWSHADLRHLSL
CHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EEVKAEVGRNDSIIQAEIGERPLTFCYPFNSYNEDVRRIVSENRIGTRIKQYAIGGEKSK
HHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCH
STVESLDKWVKELMISNDWGVTMIHGISTGYDAFTSPDILWEHFRRVKNQEYDIWVGTFR
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHH
EVAAYVKERRNVQLDIVKKESQWAVIPRLLLDKELFNEPLTMVLNKKGKGKVKVYQNGKR
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCE
LLVKKRDDKQVFDFNPYGGTIRISFF
EEEEECCCCEEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]