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The map label for this gene is glnT [H]

Identifier: 150004627

GI number: 150004627

Start: 2698076

End: 2699476

Strand: Reverse

Name: glnT [H]

Synonym: BVU_2087

Alternate gene names: 150004627

Gene position: 2699476-2698076 (Counterclockwise)

Preceding gene: 150004628

Following gene: 150004626

Centisome position: 52.28

GC content: 41.11

Gene sequence:

>1401_bases
ATGGATATACTGACCCTATTAATCAGCAGAGTGAATGATGTGCTTTGGACTTATATTTTGATCATCATGCTGTTAGGATG
TGCATTTTGGTTTACTTTTAAAACAAATTTCGTACAATTCCGCATGATAAGAGAAATGATACGTCTATTAGGTGATTCTA
CAGGCAAGACAGAAGGTCGCGAACACCATATTTCCTCTTTTCAAGCTTTTGCCGTTTCCATAGCTAGCCGCGTAGGAACA
GGCAATTTGGCAGGAGTCGCCACCGCCATTACTTTGGGTGGTCCGGGTGCAGTTTTCTGGATGTGGGTAATTGCTCTTTT
TGGGGCCAGCAGCGCTTTTATTGAGTCCACATTGGCTCAATTATATAAAGTACACGGACATAATTCTTTCGTTGGCGGGC
CAGCTTATTACATGAAAAAAGGATTAAAACAGCCGTGGATGGGAGTTTTGTTTGCCTTTTTGCTTATCTTTACTTTTGGA
TTTGCTTTTAATTCAGTACAAAGCAATACCATTTGTGCCGCTTTCGAAGAAGCATTCAATATTCCTCCCAGCCTAATGGG
AGTGATACTGACCTCGCTCACATTAATTATCATCTTCGGAGGCATTCAGCGTATTGCTAAAGTAAGTAGCATTATTGTTC
CTGTCATGGCATTGGGATACATCTTTTTATCCCTTTTCATCGTAATTGTAAATGCAAAGCATTTGCCAGAAGTTATTGAA
TTGATTATAGCGAATGCCTTTGGCTGGGAACAGGCATTAAGCGGAGGTATAGGCATGGCATTAATGCAAGGAATCAAACG
TGGATTATTCAGCAATGAGGCCGGTATGGGTTCCGCACCCAATGTAGCGGCAAGTGCCGATGTTACCCATCCTGTAAAAC
AAGGATTAATTCAGACATTGGGAGTTTTTACCGATACACTTGTCATATGTACCTGCACTGCTTTCATCATTCTTTTCAGC
GGAGTAGCCTCCACAAAAGAAGCCAATGGAATAAAACTTACTCAAATGGCACTGAATAATGAAATAGGCAACATCGGAAC
TATTTATGTGGCGGTGGCAATTCTGTTTTTCGCCTATAGCAGCATTTTGGGAAATTATTATTACGGTGAAGCCAATGTAC
GTTACATAACTCATCGGAAATGGGCTATCCCCATTTATCGTATAGCAGTGGGAGGAATGGTTATGTTTGGGGCATTGGCC
AGTCTGGAACTGGCATGGAGCCTGGCCGATATGGCAATGGGATTTATGACTATCTGTAACTTGATAGCCATTACTTTACT
AGGGAAATATGCATTCCGTCTATTGGAAGACTATCGAATTCAGAAACGTAACGGGGTCAAAAATCCAGTTTTCACCAAAG
ACAGAATGAAAGATATTGAAAATGATTTGGAATGCTGGTAA

Upstream 100 bases:

>100_bases
CAACTTGTGAACTATTAGCAGACTTTATCCGTTTTGGTCTAGTGGAACTTGTTTTCCATGGACATAAGTTCTATTTTAAA
TTAAACAAGGAATAAGTAAA

Downstream 100 bases:

>100_bases
CACAAAGCAATTCCCCCGGCATCAACCATTCAATAAAAATGATAAATAGTAAAACAAAAGGATTTTAATCAAAAATGTTT
CAGTTTTTATCGCTATCTTT

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MDILTLLISRVNDVLWTYILIIMLLGCAFWFTFKTNFVQFRMIREMIRLLGDSTGKTEGREHHISSFQAFAVSIASRVGT
GNLAGVATAITLGGPGAVFWMWVIALFGASSAFIESTLAQLYKVHGHNSFVGGPAYYMKKGLKQPWMGVLFAFLLIFTFG
FAFNSVQSNTICAAFEEAFNIPPSLMGVILTSLTLIIIFGGIQRIAKVSSIIVPVMALGYIFLSLFIVIVNAKHLPEVIE
LIIANAFGWEQALSGGIGMALMQGIKRGLFSNEAGMGSAPNVAASADVTHPVKQGLIQTLGVFTDTLVICTCTAFIILFS
GVASTKEANGIKLTQMALNNEIGNIGTIYVAVAILFFAYSSILGNYYYGEANVRYITHRKWAIPIYRIAVGGMVMFGALA
SLELAWSLADMAMGFMTICNLIAITLLGKYAFRLLEDYRIQKRNGVKNPVFTKDRMKDIENDLECW

Sequences:

>Translated_466_residues
MDILTLLISRVNDVLWTYILIIMLLGCAFWFTFKTNFVQFRMIREMIRLLGDSTGKTEGREHHISSFQAFAVSIASRVGT
GNLAGVATAITLGGPGAVFWMWVIALFGASSAFIESTLAQLYKVHGHNSFVGGPAYYMKKGLKQPWMGVLFAFLLIFTFG
FAFNSVQSNTICAAFEEAFNIPPSLMGVILTSLTLIIIFGGIQRIAKVSSIIVPVMALGYIFLSLFIVIVNAKHLPEVIE
LIIANAFGWEQALSGGIGMALMQGIKRGLFSNEAGMGSAPNVAASADVTHPVKQGLIQTLGVFTDTLVICTCTAFIILFS
GVASTKEANGIKLTQMALNNEIGNIGTIYVAVAILFFAYSSILGNYYYGEANVRYITHRKWAIPIYRIAVGGMVMFGALA
SLELAWSLADMAMGFMTICNLIAITLLGKYAFRLLEDYRIQKRNGVKNPVFTKDRMKDIENDLECW
>Mature_466_residues
MDILTLLISRVNDVLWTYILIIMLLGCAFWFTFKTNFVQFRMIREMIRLLGDSTGKTEGREHHISSFQAFAVSIASRVGT
GNLAGVATAITLGGPGAVFWMWVIALFGASSAFIESTLAQLYKVHGHNSFVGGPAYYMKKGLKQPWMGVLFAFLLIFTFG
FAFNSVQSNTICAAFEEAFNIPPSLMGVILTSLTLIIIFGGIQRIAKVSSIIVPVMALGYIFLSLFIVIVNAKHLPEVIE
LIIANAFGWEQALSGGIGMALMQGIKRGLFSNEAGMGSAPNVAASADVTHPVKQGLIQTLGVFTDTLVICTCTAFIILFS
GVASTKEANGIKLTQMALNNEIGNIGTIYVAVAILFFAYSSILGNYYYGEANVRYITHRKWAIPIYRIAVGGMVMFGALA
SLELAWSLADMAMGFMTICNLIAITLLGKYAFRLLEDYRIQKRNGVKNPVFTKDRMKDIENDLECW

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=465, Percent_Identity=43.8709677419355, Blast_Score=345, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51042; Mature: 51042

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILTLLISRVNDVLWTYILIIMLLGCAFWFTFKTNFVQFRMIREMIRLLGDSTGKTEGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
EHHISSFQAFAVSIASRVGTGNLAGVATAITLGGPGAVFWMWVIALFGASSAFIESTLAQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHH
LYKVHGHNSFVGGPAYYMKKGLKQPWMGVLFAFLLIFTFGFAFNSVQSNTICAAFEEAFN
HHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
IPPSLMGVILTSLTLIIIFGGIQRIAKVSSIIVPVMALGYIFLSLFIVIVNAKHLPEVIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIIANAFGWEQALSGGIGMALMQGIKRGLFSNEAGMGSAPNVAASADVTHPVKQGLIQTL
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
GVFTDTLVICTCTAFIILFSGVASTKEANGIKLTQMALNNEIGNIGTIYVAVAILFFAYS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHH
SILGNYYYGEANVRYITHRKWAIPIYRIAVGGMVMFGALASLELAWSLADMAMGFMTICN
HHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAITLLGKYAFRLLEDYRIQKRNGVKNPVFTKDRMKDIENDLECW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MDILTLLISRVNDVLWTYILIIMLLGCAFWFTFKTNFVQFRMIREMIRLLGDSTGKTEGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
EHHISSFQAFAVSIASRVGTGNLAGVATAITLGGPGAVFWMWVIALFGASSAFIESTLAQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHH
LYKVHGHNSFVGGPAYYMKKGLKQPWMGVLFAFLLIFTFGFAFNSVQSNTICAAFEEAFN
HHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
IPPSLMGVILTSLTLIIIFGGIQRIAKVSSIIVPVMALGYIFLSLFIVIVNAKHLPEVIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIIANAFGWEQALSGGIGMALMQGIKRGLFSNEAGMGSAPNVAASADVTHPVKQGLIQTL
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
GVFTDTLVICTCTAFIILFSGVASTKEANGIKLTQMALNNEIGNIGTIYVAVAILFFAYS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHH
SILGNYYYGEANVRYITHRKWAIPIYRIAVGGMVMFGALASLELAWSLADMAMGFMTICN
HHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAITLLGKYAFRLLEDYRIQKRNGVKNPVFTKDRMKDIENDLECW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]