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The map label for this gene is pfkA1 [H]

Identifier: 150004521

GI number: 150004521

Start: 2551639

End: 2552619

Strand: Direct

Name: pfkA1 [H]

Synonym: BVU_1971

Alternate gene names: 150004521

Gene position: 2551639-2552619 (Clockwise)

Preceding gene: 150004519

Following gene: 150004523

Centisome position: 49.42

GC content: 44.04

Gene sequence:

>981_bases
ATGAAAACAATTAAATGTATTGGAGTGCTGACATCCGGAGGGGATGCTTCTGGCATGAATGCCGCTATCCGTGCGGTAAC
CCGGAGTGCAATTTGTAACGGATTTAAAGTGAAGGGTATTTATAGAGGATATGAAGGGCTGATTAATGGAGAAGTGAAGG
AACTGACCACCCAGGATGTGAGCAGTGTTATTCAACGTGGAGGAACGATGTTGAAGACAGCCCGTTCTGCTGAATTCCAA
ACAGTAGAAGGACGCAAGAAAGCATACGATACTATGCAGCGAGAAGGAATTGATGCTTTGGTGATTATAGGTGGAAATGG
TTCTATTACAGGAGCCCGGATTTTTGCGGAAGAATATGATGTGCCTTGTATCGGGTTACCTGGTACAATAGATAATGATC
TTTATGGTACTGACTTTACCATAGGTTACGATACGGCTTTAAATACGATTGTGGAATGTGTGGATAAAATCCGTGATACG
GCAACCTCTCACGACCGTATATTCTTTGTGGAAGTGATGGGACGTGATGCCGGTTTTCTGGCTCAAAACAGTGCCATTGC
TTCTGGTGCTGAGGCAGCGATTATTCCTGAAGATCGTACGGATGTGGATCAATTGGAGAAATTTATCGGGCGCGGTTTTC
GTAAGACAAAGAATAGCAGTATTGTTATTGTTTCCGAGAGTCCGAAAGACGGAGGTGCCATGCATTATGCAGAGCGTGTC
AAGAAAGAATATCCTCAATATGATGTGCGTGTTACCATTTTAGGGCATTTGCAACGTGGAGGTTCGCCTAGTGCTTATGA
CCGTATTCTTGCCAGCCGTCTGGGGGCGGGAGCTATTGATGCCATATTGGAAGGGCAGCGCAACGTGATGATTGGCATTC
ATAACGATGAGGTGGTATATGTACCTTTTTCTGAGGCAATTAAAAAAGACAAGCCCCTTGATAAATCGCTTATCAAGGTG
CTTGATGAATTATCTATTTAA

Upstream 100 bases:

>100_bases
GCAAAAGGAATAAAAAAAGAACAATTATAATAAATATTAGTTCTATATGGTATAATGTTTGTCGTACCTTAGGTAACGAT
AAAAGCTAACATACGAATAT

Downstream 100 bases:

>100_bases
TGAAAATAATCATGAAAGGAGAAGCGGAAGTAATTCTTCTGCTTTTTCTATGATATGTGCCGGATGTAAGGCTTCCAATT
CTGTGCGCGGCCGGAATCCC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKTIKCIGVLTSGGDASGMNAAIRAVTRSAICNGFKVKGIYRGYEGLINGEVKELTTQDVSSVIQRGGTMLKTARSAEFQ
TVEGRKKAYDTMQREGIDALVIIGGNGSITGARIFAEEYDVPCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDT
ATSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLEKFIGRGFRKTKNSSIVIVSESPKDGGAMHYAERV
KKEYPQYDVRVTILGHLQRGGSPSAYDRILASRLGAGAIDAILEGQRNVMIGIHNDEVVYVPFSEAIKKDKPLDKSLIKV
LDELSI

Sequences:

>Translated_326_residues
MKTIKCIGVLTSGGDASGMNAAIRAVTRSAICNGFKVKGIYRGYEGLINGEVKELTTQDVSSVIQRGGTMLKTARSAEFQ
TVEGRKKAYDTMQREGIDALVIIGGNGSITGARIFAEEYDVPCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDT
ATSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLEKFIGRGFRKTKNSSIVIVSESPKDGGAMHYAERV
KKEYPQYDVRVTILGHLQRGGSPSAYDRILASRLGAGAIDAILEGQRNVMIGIHNDEVVYVPFSEAIKKDKPLDKSLIKV
LDELSI
>Mature_326_residues
MKTIKCIGVLTSGGDASGMNAAIRAVTRSAICNGFKVKGIYRGYEGLINGEVKELTTQDVSSVIQRGGTMLKTARSAEFQ
TVEGRKKAYDTMQREGIDALVIIGGNGSITGARIFAEEYDVPCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDT
ATSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLEKFIGRGFRKTKNSSIVIVSESPKDGGAMHYAERV
KKEYPQYDVRVTILGHLQRGGSPSAYDRILASRLGAGAIDAILEGQRNVMIGIHNDEVVYVPFSEAIKKDKPLDKSLIKV
LDELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=348, Percent_Identity=41.3793103448276, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI11321601, Length=347, Percent_Identity=40.9221902017291, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI266453768, Length=348, Percent_Identity=40.5172413793103, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI266453748, Length=348, Percent_Identity=40.5172413793103, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI4505749, Length=348, Percent_Identity=40.5172413793103, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI266453619, Length=348, Percent_Identity=40.5172413793103, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI1790350, Length=308, Percent_Identity=49.6753246753247, Blast_Score=267, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI25147584, Length=352, Percent_Identity=42.3295454545455, Blast_Score=240, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=39.1585760517799, Blast_Score=200, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=339, Percent_Identity=29.4985250737463, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=343, Percent_Identity=40.5247813411079, Blast_Score=222, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6323861, Length=355, Percent_Identity=36.056338028169, Blast_Score=203, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=40.9221902017291, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652337, Length=347, Percent_Identity=40.6340057636888, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17647809, Length=347, Percent_Identity=40.6340057636888, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35282; Mature: 35282

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIKCIGVLTSGGDASGMNAAIRAVTRSAICNGFKVKGIYRGYEGLINGEVKELTTQDV
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCCHHHHHHHHHH
SSVIQRGGTMLKTARSAEFQTVEGRKKAYDTMQREGIDALVIIGGNGSITGARIFAEEYD
HHHHHCCCCEEEHHCCCCCEECCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHCC
VPCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTATSHDRIFFVEVMGRDAGFL
CCEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCE
AQNSAIASGAEAAIIPEDRTDVDQLEKFIGRGFRKTKNSSIVIVSESPKDGGAMHYAERV
ECCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
KKEYPQYDVRVTILGHLQRGGSPSAYDRILASRLGAGAIDAILEGQRNVMIGIHNDEVVY
HHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCEEE
VPFSEAIKKDKPLDKSLIKVLDELSI
EEHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKTIKCIGVLTSGGDASGMNAAIRAVTRSAICNGFKVKGIYRGYEGLINGEVKELTTQDV
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCCHHHHHHHHHH
SSVIQRGGTMLKTARSAEFQTVEGRKKAYDTMQREGIDALVIIGGNGSITGARIFAEEYD
HHHHHCCCCEEEHHCCCCCEECCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHCC
VPCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTATSHDRIFFVEVMGRDAGFL
CCEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCE
AQNSAIASGAEAAIIPEDRTDVDQLEKFIGRGFRKTKNSSIVIVSESPKDGGAMHYAERV
ECCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
KKEYPQYDVRVTILGHLQRGGSPSAYDRILASRLGAGAIDAILEGQRNVMIGIHNDEVVY
HHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCEEE
VPFSEAIKKDKPLDKSLIKVLDELSI
EEHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]