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The map label for this gene is rsmF [H]

Identifier: 150004504

GI number: 150004504

Start: 2530936

End: 2532363

Strand: Direct

Name: rsmF [H]

Synonym: BVU_1954

Alternate gene names: 150004504

Gene position: 2530936-2532363 (Clockwise)

Preceding gene: 150004498

Following gene: 150004506

Centisome position: 49.02

GC content: 44.61

Gene sequence:

>1428_bases
ATGAAATTACCATCAGAATTTGAAGCAAGAACCCTGGAATGGATGGGAGAAGAAACTTATCGCGCGTTGGAAGCGGCTTT
GCAGACGGAGCCTCCTGTCAGTATTCGTGTCAATCGTACGAAGTGGAGTGGGGAAGTTGCAGGAGAACCGGTACTTTGGG
CATCTGCAGGGGTTTATTTATCGCAACGGTCCACTTTTACATTCGATCCGTTGTTTCATGCTGGTTGCTATTATGTGCAA
GAAGCATCTTCCATGTTTGTGGAACAGGTATTACGCACTTATATTACCGGACCTGTAGTGATGCTCGATTTGTGTGCTGC
TCCCGGTGGCAAGTCTACTCATGCGCGTTCAGTTCTCCCCGTAGGTAGTTTGTTGGTGGCTAATGAGGTGATGCGTAACC
GTTCTCAGGTATTGGCTGAAAATTTGATAAAGTGGGGAAATGCAGAAGTTGTGGTGACGAACAACGATCCGGCCGATTTC
ACTTCTTTGACAGAGGTGTTTGATGTTGTCTTGGCAGATGTGCCTTGTTCCGGTGAGGGGATGTTCCGGAAGGATCCTGT
GGCTGTAGAGGAATGGAGTACGGACAATGTGCAGGTTTGTTGGCAACGTCAGCGTAGGATTTTAGCGGATATATGGCCGG
CTTTGAAACCCGGTGGTCTGCTGATTTATAGTACCTGCACTTATAATAGGGAAGAAGATGAGGAAAATGTGGCTTGGATT
GCTGATGAATTGGGTGCGGAGAAACTGAAAGTGCCAGTGGCGTCGGAATGGGGGATAATAGGAAATTTGGCGGGACAGGA
TTTTCCTGTTTATCGTTTTTTGCCTCATAGAGTGAAAGGAGAAGGATTTTTCTTGGCTGTATTGAGGAAGAGTGCTGCCA
TTTCTCATGTTGTTCCTTGTATTCGTTGTCAGGATGACAAAGAGAAAATAACAAAAAAGAAGAGAAAAGGAGAAAAGGGG
AGCTTGTCTCAGGTTGTTCCTTTTCCCAAAGAAGTAAAAAGTTGGTTAAAGCAGGCGGAGGATTTCCGATTTGAGGTAAG
AGGCACGAAGGTGATAGCCTTCCCCAATGTCCATCTTTCAGAATATGATCTGTTCCGTCAGGAATTAAAAGTTGTTCATG
CGGGTGTTACTATAGGTGAGTTGAAAGGAAAAGACGTTATACCGGATCATTCGTTGGCAATGAGCACATTGCTGAATCAT
GATGGTTTCTCTCGTTTTGAATTGACCTATGAACAGGCTATTGCTTATTTGCGTAAAGAAGCGATCACATTGGATGCTTC
TGTTCCGCGTGGTTATATACTTTTGACTTATAAAAACATTCCATTAGGATTTGCCAAGAACATTGGCAATCGGGCTAATA
ACCTCTATCCGCAGGAATGGCGTATCCGAAGCGGTTATTTACCCGAAGAACTTTCTTTTGTATGCTAA

Upstream 100 bases:

>100_bases
GTTAGACGGAACGAATAAGGAAATAGGTTGCAAATAGACAGAAAATTTTCTGATAATCCCAAAATATCATCGTATTTTTG
TAACATAAAAAGGAAGGAAC

Downstream 100 bases:

>100_bases
AAGGAGCCATAGATTTGTTCGGGAAGATTGTGCATATTGTTTTTCATAGACTGGCGCAATGCTTCTCCGGGTACAGACTT
GTTTGTATTCTATAATATAC

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKLPSEFEARTLEWMGEETYRALEAALQTEPPVSIRVNRTKWSGEVAGEPVLWASAGVYLSQRSTFTFDPLFHAGCYYVQ
EASSMFVEQVLRTYITGPVVMLDLCAAPGGKSTHARSVLPVGSLLVANEVMRNRSQVLAENLIKWGNAEVVVTNNDPADF
TSLTEVFDVVLADVPCSGEGMFRKDPVAVEEWSTDNVQVCWQRQRRILADIWPALKPGGLLIYSTCTYNREEDEENVAWI
ADELGAEKLKVPVASEWGIIGNLAGQDFPVYRFLPHRVKGEGFFLAVLRKSAAISHVVPCIRCQDDKEKITKKKRKGEKG
SLSQVVPFPKEVKSWLKQAEDFRFEVRGTKVIAFPNVHLSEYDLFRQELKVVHAGVTIGELKGKDVIPDHSLAMSTLLNH
DGFSRFELTYEQAIAYLRKEAITLDASVPRGYILLTYKNIPLGFAKNIGNRANNLYPQEWRIRSGYLPEELSFVC

Sequences:

>Translated_475_residues
MKLPSEFEARTLEWMGEETYRALEAALQTEPPVSIRVNRTKWSGEVAGEPVLWASAGVYLSQRSTFTFDPLFHAGCYYVQ
EASSMFVEQVLRTYITGPVVMLDLCAAPGGKSTHARSVLPVGSLLVANEVMRNRSQVLAENLIKWGNAEVVVTNNDPADF
TSLTEVFDVVLADVPCSGEGMFRKDPVAVEEWSTDNVQVCWQRQRRILADIWPALKPGGLLIYSTCTYNREEDEENVAWI
ADELGAEKLKVPVASEWGIIGNLAGQDFPVYRFLPHRVKGEGFFLAVLRKSAAISHVVPCIRCQDDKEKITKKKRKGEKG
SLSQVVPFPKEVKSWLKQAEDFRFEVRGTKVIAFPNVHLSEYDLFRQELKVVHAGVTIGELKGKDVIPDHSLAMSTLLNH
DGFSRFELTYEQAIAYLRKEAITLDASVPRGYILLTYKNIPLGFAKNIGNRANNLYPQEWRIRSGYLPEELSFVC
>Mature_475_residues
MKLPSEFEARTLEWMGEETYRALEAALQTEPPVSIRVNRTKWSGEVAGEPVLWASAGVYLSQRSTFTFDPLFHAGCYYVQ
EASSMFVEQVLRTYITGPVVMLDLCAAPGGKSTHARSVLPVGSLLVANEVMRNRSQVLAENLIKWGNAEVVVTNNDPADF
TSLTEVFDVVLADVPCSGEGMFRKDPVAVEEWSTDNVQVCWQRQRRILADIWPALKPGGLLIYSTCTYNREEDEENVAWI
ADELGAEKLKVPVASEWGIIGNLAGQDFPVYRFLPHRVKGEGFFLAVLRKSAAISHVVPCIRCQDDKEKITKKKRKGEKG
SLSQVVPFPKEVKSWLKQAEDFRFEVRGTKVIAFPNVHLSEYDLFRQELKVVHAGVTIGELKGKDVIPDHSLAMSTLLNH
DGFSRFELTYEQAIAYLRKEAITLDASVPRGYILLTYKNIPLGFAKNIGNRANNLYPQEWRIRSGYLPEELSFVC

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=261, Percent_Identity=27.9693486590038, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI76150623, Length=261, Percent_Identity=27.9693486590038, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI32698918, Length=175, Percent_Identity=30.8571428571429, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI39995082, Length=281, Percent_Identity=27.0462633451957, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI301336155, Length=281, Percent_Identity=27.0462633451957, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI87081985, Length=430, Percent_Identity=30.9302325581395, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI2367212, Length=288, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=284, Percent_Identity=28.169014084507, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25143471, Length=184, Percent_Identity=36.4130434782609, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324268, Length=235, Percent_Identity=30.2127659574468, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319447, Length=247, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI22024126, Length=302, Percent_Identity=29.8013245033113, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355201, Length=311, Percent_Identity=28.2958199356913, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53398; Mature: 53398

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPSEFEARTLEWMGEETYRALEAALQTEPPVSIRVNRTKWSGEVAGEPVLWASAGVYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEECCEEE
SQRSTFTFDPLFHAGCYYVQEASSMFVEQVLRTYITGPVVMLDLCAAPGGKSTHARSVLP
ECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
VGSLLVANEVMRNRSQVLAENLIKWGNAEVVVTNNDPADFTSLTEVFDVVLADVPCSGEG
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
MFRKDPVAVEEWSTDNVQVCWQRQRRILADIWPALKPGGLLIYSTCTYNREEDEENVAWI
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHH
ADELGAEKLKVPVASEWGIIGNLAGQDFPVYRFLPHRVKGEGFFLAVLRKSAAISHVVPC
HHHCCCHHEECCCCCCCCCEECCCCCCCCHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHH
IRCQDDKEKITKKKRKGEKGSLSQVVPFPKEVKSWLKQAEDFRFEVRGTKVIAFPNVHLS
HCCCCHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHCCEEEECCEEEEEECCCCCH
EYDLFRQELKVVHAGVTIGELKGKDVIPDHSLAMSTLLNHDGFSRFELTYEQAIAYLRKE
HHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
AITLDASVPRGYILLTYKNIPLGFAKNIGNRANNLYPQEWRIRSGYLPEELSFVC
HEEEECCCCCCEEEEEEECCCCHHHHHCCCHHCCCCCCCEEECCCCCCHHHHCCC
>Mature Secondary Structure
MKLPSEFEARTLEWMGEETYRALEAALQTEPPVSIRVNRTKWSGEVAGEPVLWASAGVYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEECCEEE
SQRSTFTFDPLFHAGCYYVQEASSMFVEQVLRTYITGPVVMLDLCAAPGGKSTHARSVLP
ECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
VGSLLVANEVMRNRSQVLAENLIKWGNAEVVVTNNDPADFTSLTEVFDVVLADVPCSGEG
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
MFRKDPVAVEEWSTDNVQVCWQRQRRILADIWPALKPGGLLIYSTCTYNREEDEENVAWI
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHH
ADELGAEKLKVPVASEWGIIGNLAGQDFPVYRFLPHRVKGEGFFLAVLRKSAAISHVVPC
HHHCCCHHEECCCCCCCCCEECCCCCCCCHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHH
IRCQDDKEKITKKKRKGEKGSLSQVVPFPKEVKSWLKQAEDFRFEVRGTKVIAFPNVHLS
HCCCCHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHCCEEEECCEEEEEECCCCCH
EYDLFRQELKVVHAGVTIGELKGKDVIPDHSLAMSTLLNHDGFSRFELTYEQAIAYLRKE
HHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
AITLDASVPRGYILLTYKNIPLGFAKNIGNRANNLYPQEWRIRSGYLPEELSFVC
HEEEECCCCCCEEEEEEECCCCHHHHHCCCHHCCCCCCCEEECCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA