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The map label for this gene is ksgA

Identifier: 150004476

GI number: 150004476

Start: 2503013

End: 2503855

Strand: Reverse

Name: ksgA

Synonym: BVU_1925

Alternate gene names: 150004476

Gene position: 2503855-2503013 (Counterclockwise)

Preceding gene: 150004479

Following gene: 150004475

Centisome position: 48.49

GC content: 43.18

Gene sequence:

>843_bases
ATGGGATTAGTAAAGCCAAAAAAATTTCTAGGACAACACTTCTTGAAAGATCTCAGTATTGCAAAAGATATAGCCGATAC
GGTAGACGTGTGCCCTGACTTGCCAATTCTGGAAGTAGGACCGGGGATGGGGGTACTAACCCAGTTTATTATGCAGAAAA
ACCGTCCGGTAAAAGTGGTGGAACTGGACTATGAATCTGTAGCCTATCTCCGTGAGAACTTCCCTGCCCTGGAAGATAAT
ATCATTGAAGACGACTTCTTGAAACTGAACCTCGAAAAATTATTTGACGGAAAACCTTTTGTCCTGACCGGCAACTATCC
TTATAATATATCCAGCCAGATATTCTTTAAAATGCTGGATTACAAAGACCTGATACCCTGTTGTACCGGAATGATCCAAA
AAGAAGTGGCCGAACGCATTGCCGCCGGTCCCGGAAGCAAGACATACGGCATTCTCAGTATCCTGATACAAGCCTGGTAC
AAAGTAGAATACCTGTTCACCGTACACGAACATGTATTCAATCCGCCTCCCAAGGTGAAAAGTGCGGTCATCCGTATGAC
ACGCAATGAAACGACGGAACTGGGATGCAATGAAAAATTGTTCAAGCTCATTGTAAAAACAACATTCAACCAACGGCGTA
AAACTTTGAGAAACTCTATTTCTTCCATTTTAGACAAAGAGAACCCATTGAGCGCAGACCCCATTTTCAACAAACGCCCG
GAACAGTTGTCTGTACAAGAGTTTATCGAACTGACCAACCAGGTGGAAGCGGCGCTGAAAAACAAGACGGATATTGTCTA
TAGCAATGATATCGCCCGGAAAGGAACAAACAAGAAAGAATAA

Upstream 100 bases:

>100_bases
AAAATCTGTGTATATTAATTTAGAATGTTTACCTTTGCACGATGCAAAGATACTATCTTAGCGAAATAAACCTATTTATT
AACGATAGAAAAATAACGGA

Downstream 100 bases:

>100_bases
CACCTATTTATTATATTAACGGAAGAATAGGATGAATAAAGAGGAATTAGAAAAAGTAGAGCAGCTGATCGAGCAAAAAG
ACTCAGAGCAACTGAAGGAC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MGLVKPKKFLGQHFLKDLSIAKDIADTVDVCPDLPILEVGPGMGVLTQFIMQKNRPVKVVELDYESVAYLRENFPALEDN
IIEDDFLKLNLEKLFDGKPFVLTGNYPYNISSQIFFKMLDYKDLIPCCTGMIQKEVAERIAAGPGSKTYGILSILIQAWY
KVEYLFTVHEHVFNPPPKVKSAVIRMTRNETTELGCNEKLFKLIVKTTFNQRRKTLRNSISSILDKENPLSADPIFNKRP
EQLSVQEFIELTNQVEAALKNKTDIVYSNDIARKGTNKKE

Sequences:

>Translated_280_residues
MGLVKPKKFLGQHFLKDLSIAKDIADTVDVCPDLPILEVGPGMGVLTQFIMQKNRPVKVVELDYESVAYLRENFPALEDN
IIEDDFLKLNLEKLFDGKPFVLTGNYPYNISSQIFFKMLDYKDLIPCCTGMIQKEVAERIAAGPGSKTYGILSILIQAWY
KVEYLFTVHEHVFNPPPKVKSAVIRMTRNETTELGCNEKLFKLIVKTTFNQRRKTLRNSISSILDKENPLSADPIFNKRP
EQLSVQEFIELTNQVEAALKNKTDIVYSNDIARKGTNKKE
>Mature_279_residues
GLVKPKKFLGQHFLKDLSIAKDIADTVDVCPDLPILEVGPGMGVLTQFIMQKNRPVKVVELDYESVAYLRENFPALEDNI
IEDDFLKLNLEKLFDGKPFVLTGNYPYNISSQIFFKMLDYKDLIPCCTGMIQKEVAERIAAGPGSKTYGILSILIQAWYK
VEYLFTVHEHVFNPPPKVKSAVIRMTRNETTELGCNEKLFKLIVKTTFNQRRKTLRNSISSILDKENPLSADPIFNKRPE
QLSVQEFIELTNQVEAALKNKTDIVYSNDIARKGTNKKE

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=218, Percent_Identity=35.3211009174312, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI156415992, Length=281, Percent_Identity=28.8256227758007, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786236, Length=253, Percent_Identity=33.596837944664, Blast_Score=143, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25146882, Length=181, Percent_Identity=34.8066298342541, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25141369, Length=256, Percent_Identity=23.4375, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324989, Length=218, Percent_Identity=32.1100917431193, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21358017, Length=189, Percent_Identity=35.4497354497354, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21357273, Length=288, Percent_Identity=26.3888888888889, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BACV8 (A6L1N4)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299220.1
- ProteinModelPortal:   A6L1N4
- SMR:   A6L1N4
- STRING:   A6L1N4
- GeneID:   5302891
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1925
- NMPDR:   fig|435590.6.peg.1810
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31909; Mature: 31778

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 13-13 BINDING 15-15 BINDING 40-40 BINDING 61-61 BINDING 85-85 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLVKPKKFLGQHFLKDLSIAKDIADTVDVCPDLPILEVGPGMGVLTQFIMQKNRPVKVV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEEE
ELDYESVAYLRENFPALEDNIIEDDFLKLNLEKLFDGKPFVLTGNYPYNISSQIFFKMLD
EECHHHHHHHHHCCCCHHHCCCCCHHHHEEHHHHHCCCCEEEECCCCCCCHHHHHHHHHH
YKDLIPCCTGMIQKEVAERIAAGPGSKTYGILSILIQAWYKVEYLFTVHEHVFNPPPKVK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SAVIRMTRNETTELGCNEKLFKLIVKTTFNQRRKTLRNSISSILDKENPLSADPIFNKRP
HHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EQLSVQEFIELTNQVEAALKNKTDIVYSNDIARKGTNKKE
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCCC
>Mature Secondary Structure 
GLVKPKKFLGQHFLKDLSIAKDIADTVDVCPDLPILEVGPGMGVLTQFIMQKNRPVKVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEEE
ELDYESVAYLRENFPALEDNIIEDDFLKLNLEKLFDGKPFVLTGNYPYNISSQIFFKMLD
EECHHHHHHHHHCCCCHHHCCCCCHHHHEEHHHHHCCCCEEEECCCCCCCHHHHHHHHHH
YKDLIPCCTGMIQKEVAERIAAGPGSKTYGILSILIQAWYKVEYLFTVHEHVFNPPPKVK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SAVIRMTRNETTELGCNEKLFKLIVKTTFNQRRKTLRNSISSILDKENPLSADPIFNKRP
HHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EQLSVQEFIELTNQVEAALKNKTDIVYSNDIARKGTNKKE
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA