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The map label for this gene is mltD [H]

Identifier: 150004464

GI number: 150004464

Start: 2488982

End: 2490244

Strand: Reverse

Name: mltD [H]

Synonym: BVU_1912

Alternate gene names: 150004464

Gene position: 2490244-2488982 (Counterclockwise)

Preceding gene: 150004465

Following gene: 150004463

Centisome position: 48.23

GC content: 44.1

Gene sequence:

>1263_bases
ATGAAAAACTTTTTAATTGGTTTATTTACATTTATAGGAACAACGGGAATAAGCGCACAAACCCAAAGTATTACCGTTCA
CTCGCAAACTACAGGAAAAGAAGAAATTATTGATGTTCCTGAAAGTATGCAGTCAGAAATGGACAGTCTTTACTGGGACT
GGCAATCTAAGAATCACATCACATTGGGAGAGAACTGCACAATGGCCTCTACCAACCCTGTTGTAAGTGATTCCATCTAT
ATGGATCGTCTGTCACGTATTCCCTCTATCATAGAAATGCCTTACAATGAGGCAGTACGCAAATTCATTGATATGTATGC
CGTCCGTCTGCGCAATAAAGTATCCTTTATGCTTGCGGCAACCAATTTTTATATGCCTATCTTTGAAGAAGCACTTGATT
TATACGACCTTCCTCAAGAACTGAAATATCTGCCGATTATAGAGTCCGCCTTAAATCCTGTCGCTGTTTCCCGCCAGGGA
GCTACCGGCTTATGGCAGTTTATGCTGGGTACAGGAAAAATATACGGGCTGAAGAACAATAGCCTGATAGACGAAAGACG
GGACCCGGTCAAATCAACTTGGGCGGCAGCACGTTACCTGAAAGACCTGTACGACATCTATCAAGACTGGAATCTGGTAT
TGGCAGCTTACAACTGCGGGCCGGGCACTATCAACAAAGCCATACGCCGTGCCGGAGGAGCCACGGATTATTGGACAATC
TACAACTATCTGCCCAAAGAAACCCGTGGGTACGTTCCTGCTTTCATAGCGGCAAACTACATCATGACCTATTATTGCGA
ACATGGCATCTGTCCCATGGAAACACAGCTTCCCAACGCTACAGACACCATTCACATTAACAAAGACCTCCACTTACAAC
AAGTGGCAGAAGTATGCAACATCAACCTAGACCAGCTTCGCAGCCTGAACCCCCAATACAAAAAGGATATTATTCCCGGT
AACAGCGAGTTGTGCGCACTTCGTCTGCCCAATAACTTTGTCAGTACTTTCATTGACCGCCAAGACAGCGTGTTCGCTTA
TAAGCCCAATGAATATCTGACCAAAAGAAAAACGGTAGCCATCAAGGAAACAACCAGTAGCAGAAACCGCTCATCCAAGG
GAACTCTTTATCATAAAATCAAACAAGGAGACACATTGGGAGGAATTGCTGCCAAATACCACGTATCCATCAGCCAATTA
CGTAATCTAAACGGTATAAAGGGCAATAATATCAGAGCCGGAAGAAGTTTGCGGATCAGATGA

Upstream 100 bases:

>100_bases
AAAGACTTAGGTCTTCAAATACAACCTGTTATTTTTAATGACGGCCGGAGCAGGATACCTAATACAATAGGCCTGCAATG
CAGCTTCAAATTTTAATCGC

Downstream 100 bases:

>100_bases
TTGATAAAAAATACAGATTTAACACTATACATTTTCTGAAAATACTTGCTTGAATATGTTTTTTGTATATTTTTGCAGTA
TAGATTATAGGAGGCAAAGA

Product: glycoside hydrolase family protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKNFLIGLFTFIGTTGISAQTQSITVHSQTTGKEEIIDVPESMQSEMDSLYWDWQSKNHITLGENCTMASTNPVVSDSIY
MDRLSRIPSIIEMPYNEAVRKFIDMYAVRLRNKVSFMLAATNFYMPIFEEALDLYDLPQELKYLPIIESALNPVAVSRQG
ATGLWQFMLGTGKIYGLKNNSLIDERRDPVKSTWAAARYLKDLYDIYQDWNLVLAAYNCGPGTINKAIRRAGGATDYWTI
YNYLPKETRGYVPAFIAANYIMTYYCEHGICPMETQLPNATDTIHINKDLHLQQVAEVCNINLDQLRSLNPQYKKDIIPG
NSELCALRLPNNFVSTFIDRQDSVFAYKPNEYLTKRKTVAIKETTSSRNRSSKGTLYHKIKQGDTLGGIAAKYHVSISQL
RNLNGIKGNNIRAGRSLRIR

Sequences:

>Translated_420_residues
MKNFLIGLFTFIGTTGISAQTQSITVHSQTTGKEEIIDVPESMQSEMDSLYWDWQSKNHITLGENCTMASTNPVVSDSIY
MDRLSRIPSIIEMPYNEAVRKFIDMYAVRLRNKVSFMLAATNFYMPIFEEALDLYDLPQELKYLPIIESALNPVAVSRQG
ATGLWQFMLGTGKIYGLKNNSLIDERRDPVKSTWAAARYLKDLYDIYQDWNLVLAAYNCGPGTINKAIRRAGGATDYWTI
YNYLPKETRGYVPAFIAANYIMTYYCEHGICPMETQLPNATDTIHINKDLHLQQVAEVCNINLDQLRSLNPQYKKDIIPG
NSELCALRLPNNFVSTFIDRQDSVFAYKPNEYLTKRKTVAIKETTSSRNRSSKGTLYHKIKQGDTLGGIAAKYHVSISQL
RNLNGIKGNNIRAGRSLRIR
>Mature_420_residues
MKNFLIGLFTFIGTTGISAQTQSITVHSQTTGKEEIIDVPESMQSEMDSLYWDWQSKNHITLGENCTMASTNPVVSDSIY
MDRLSRIPSIIEMPYNEAVRKFIDMYAVRLRNKVSFMLAATNFYMPIFEEALDLYDLPQELKYLPIIESALNPVAVSRQG
ATGLWQFMLGTGKIYGLKNNSLIDERRDPVKSTWAAARYLKDLYDIYQDWNLVLAAYNCGPGTINKAIRRAGGATDYWTI
YNYLPKETRGYVPAFIAANYIMTYYCEHGICPMETQLPNATDTIHINKDLHLQQVAEVCNINLDQLRSLNPQYKKDIIPG
NSELCALRLPNNFVSTFIDRQDSVFAYKPNEYLTKRKTVAIKETTSSRNRSSKGTLYHKIKQGDTLGGIAAKYHVSISQL
RNLNGIKGNNIRAGRSLRIR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=334, Percent_Identity=30.8383233532934, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47704; Mature: 47704

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFLIGLFTFIGTTGISAQTQSITVHSQTTGKEEIIDVPESMQSEMDSLYWDWQSKNHI
CCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHCCCHHHHHHHHHHEECCCCCCCE
TLGENCTMASTNPVVSDSIYMDRLSRIPSIIEMPYNEAVRKFIDMYAVRLRNKVSFMLAA
EECCCCEECCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEE
TNFYMPIFEEALDLYDLPQELKYLPIIESALNPVAVSRQGATGLWQFMLGTGKIYGLKNN
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCEEEEECCC
SLIDERRDPVKSTWAAARYLKDLYDIYQDWNLVLAAYNCGPGTINKAIRRAGGATDYWTI
CCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHH
YNYLPKETRGYVPAFIAANYIMTYYCEHGICPMETQLPNATDTIHINKDLHLQQVAEVCN
HHCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHC
INLDQLRSLNPQYKKDIIPGNSELCALRLPNNFVSTFIDRQDSVFAYKPNEYLTKRKTVA
CCHHHHHCCCCHHHHCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEECCHHHHHHCCEEE
IKETTSSRNRSSKGTLYHKIKQGDTLGGIAAKYHVSISQLRNLNGIKGNNIRAGRSLRIR
EEECCCCCCCCCCCCEEEEECCCCCCCCCHHEEEEEHHHHHCCCCCCCCCEECCCCEECC
>Mature Secondary Structure
MKNFLIGLFTFIGTTGISAQTQSITVHSQTTGKEEIIDVPESMQSEMDSLYWDWQSKNHI
CCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHCCCHHHHHHHHHHEECCCCCCCE
TLGENCTMASTNPVVSDSIYMDRLSRIPSIIEMPYNEAVRKFIDMYAVRLRNKVSFMLAA
EECCCCEECCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEE
TNFYMPIFEEALDLYDLPQELKYLPIIESALNPVAVSRQGATGLWQFMLGTGKIYGLKNN
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCEEEEECCC
SLIDERRDPVKSTWAAARYLKDLYDIYQDWNLVLAAYNCGPGTINKAIRRAGGATDYWTI
CCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHH
YNYLPKETRGYVPAFIAANYIMTYYCEHGICPMETQLPNATDTIHINKDLHLQQVAEVCN
HHCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHC
INLDQLRSLNPQYKKDIIPGNSELCALRLPNNFVSTFIDRQDSVFAYKPNEYLTKRKTVA
CCHHHHHCCCCHHHHCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEECCHHHHHHCCEEE
IKETTSSRNRSSKGTLYHKIKQGDTLGGIAAKYHVSISQLRNLNGIKGNNIRAGRSLRIR
EEECCCCCCCCCCCCEEEEECCCCCCCCCHHEEEEEHHHHHCCCCCCCCCEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]