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The map label for this gene is cls [H]

Identifier: 150004455

GI number: 150004455

Start: 2477104

End: 2478540

Strand: Direct

Name: cls [H]

Synonym: BVU_1903

Alternate gene names: 150004455

Gene position: 2477104-2478540 (Clockwise)

Preceding gene: 150004454

Following gene: 150004459

Centisome position: 47.98

GC content: 42.73

Gene sequence:

>1437_bases
ATGGTAGAGTGGAGTATCTTTAATGAAATCTTTGCTGTTGCTTTTAACGTGCTTTATTTTGTGATTATCATCGGAACTAT
CTTTATAGTGATACTTGATAATCGTAATCCCGTTAAAACAATGGCATGGGTACTTGTGCTCTTTTTCCTGCCCATTGTGG
GGCTTGTGTTCTATTTCTTTTTCGGCAGAAGCACACGTAAGGAGAAATTGATCAGCAAGAAAGGGTTCACCCGTCTCATA
AAACGTCCCATGGCAGAATATCAGGCGCAAAAGGCGTTTAAGTGTCCTGATGAACAGCACCAGTTGATGCGTTTTTTCCG
GAAAGTGAATAATGCTTTGCCCTTTGAAGGAAATGCGACGGAAGTTTTTACGGATGGTTATTCCATGCTGTATTCATTGA
TGAAAGAAATCAGTAAGGCAAAGCATCACATCCATTTGCAGTTTTATATTTTTGAGGATGATCCGGCAGGCAGGTTGCTG
CGCGATTTGTTGATAGATAAAGCGCGGCAGGGAGTAGAAGTGCGTTTGCTATATGACGATGTGGGCTGCTGGAAAGTTCC
CCATGCTTTTTTTGATGAAATGCGGGGAGCAGGTATTGAGGCACGCAGTTTTTTGAAAGTCCGTTTTCCTTTATTTACCA
GTAAGGTAAATTATCGTAATCACCGTAAAATAGTAGTAATCGACGGTCGTGTGGCTTATACGGGAGGGATGAACATTGCT
TTGCGCTATATGAAAGGCTTTCCATGGGGGATATGGCGGGATACTCACATTAAAATAGAAGGAAAAGCGGTATATGGGTT
ACAGACTGCTTTTTTGACCGATTGGTATGTAGTAGACCGTACCCTTATCACTTCCTCCCGTTATTTCCCCGAAGTCGGAA
TCTTTGGAAAGGCTCTGATACAGATTGTCACTTCCGATCCTGTAGGCGAATGGCGGGATATTATGCAAGGGCTACTGATA
GCTATCAGCAGCTCCCGAAAGTATTTTTATATACAAACACCTTACTTGTTGCCTACCGAACCTATTCTGATGGCATTGAA
AACGGCAGCTTTGGCAGGCGTGGATGTTCGCATTATGATACCGGCACGTGCCGATACATGGATAACACATTTAGGTTCGT
TATCTTATTTGGATGATATCATGCGTGCGGGTGTCAGGATTTACCTTTATCAGAAAGGTTTTTTGCATTCCAAGCTGATG
GTCAGTGATGATACTCTTTCTACGGTAGGTTCTACAAATATGGACTTCCGTAGCTTTGAGCATAATTTTGAGGTGAATGC
CTTTATGTATGATCCGTCCTCAGCATTGCTGTTGAAAGGTATTTTCCTGCAAGACCAGAAGGACGCCATCCTGTTGCAGA
GAAAAACATGGATAAAGCGTCCTTGGTATCAAAAAGCACAAGAATCTATTGTCCGGTTGCTGGCACCGTTGCTTTGA

Upstream 100 bases:

>100_bases
CTTTTTAAATGAAACTATATCTTAATCTTTTCCCAATCTATATTGTTATAGGTTGGGATTTTTTTTATAAGTTTGCTTAT
AAGTAAATTACTGGAATTAT

Downstream 100 bases:

>100_bases
AGAAAGAGAAGTTCACACTGCCATATGCACGGCGCTCAATAAAATGGGGGTCATCCTCAAAATGATTCTCTTTGCCATGT
TCCAGTACCAGTAATCCGTC

Product: putative cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MVEWSIFNEIFAVAFNVLYFVIIIGTIFIVILDNRNPVKTMAWVLVLFFLPIVGLVFYFFFGRSTRKEKLISKKGFTRLI
KRPMAEYQAQKAFKCPDEQHQLMRFFRKVNNALPFEGNATEVFTDGYSMLYSLMKEISKAKHHIHLQFYIFEDDPAGRLL
RDLLIDKARQGVEVRLLYDDVGCWKVPHAFFDEMRGAGIEARSFLKVRFPLFTSKVNYRNHRKIVVIDGRVAYTGGMNIA
LRYMKGFPWGIWRDTHIKIEGKAVYGLQTAFLTDWYVVDRTLITSSRYFPEVGIFGKALIQIVTSDPVGEWRDIMQGLLI
AISSSRKYFYIQTPYLLPTEPILMALKTAALAGVDVRIMIPARADTWITHLGSLSYLDDIMRAGVRIYLYQKGFLHSKLM
VSDDTLSTVGSTNMDFRSFEHNFEVNAFMYDPSSALLLKGIFLQDQKDAILLQRKTWIKRPWYQKAQESIVRLLAPLL

Sequences:

>Translated_478_residues
MVEWSIFNEIFAVAFNVLYFVIIIGTIFIVILDNRNPVKTMAWVLVLFFLPIVGLVFYFFFGRSTRKEKLISKKGFTRLI
KRPMAEYQAQKAFKCPDEQHQLMRFFRKVNNALPFEGNATEVFTDGYSMLYSLMKEISKAKHHIHLQFYIFEDDPAGRLL
RDLLIDKARQGVEVRLLYDDVGCWKVPHAFFDEMRGAGIEARSFLKVRFPLFTSKVNYRNHRKIVVIDGRVAYTGGMNIA
LRYMKGFPWGIWRDTHIKIEGKAVYGLQTAFLTDWYVVDRTLITSSRYFPEVGIFGKALIQIVTSDPVGEWRDIMQGLLI
AISSSRKYFYIQTPYLLPTEPILMALKTAALAGVDVRIMIPARADTWITHLGSLSYLDDIMRAGVRIYLYQKGFLHSKLM
VSDDTLSTVGSTNMDFRSFEHNFEVNAFMYDPSSALLLKGIFLQDQKDAILLQRKTWIKRPWYQKAQESIVRLLAPLL
>Mature_478_residues
MVEWSIFNEIFAVAFNVLYFVIIIGTIFIVILDNRNPVKTMAWVLVLFFLPIVGLVFYFFFGRSTRKEKLISKKGFTRLI
KRPMAEYQAQKAFKCPDEQHQLMRFFRKVNNALPFEGNATEVFTDGYSMLYSLMKEISKAKHHIHLQFYIFEDDPAGRLL
RDLLIDKARQGVEVRLLYDDVGCWKVPHAFFDEMRGAGIEARSFLKVRFPLFTSKVNYRNHRKIVVIDGRVAYTGGMNIA
LRYMKGFPWGIWRDTHIKIEGKAVYGLQTAFLTDWYVVDRTLITSSRYFPEVGIFGKALIQIVTSDPVGEWRDIMQGLLI
AISSSRKYFYIQTPYLLPTEPILMALKTAALAGVDVRIMIPARADTWITHLGSLSYLDDIMRAGVRIYLYQKGFLHSKLM
VSDDTLSTVGSTNMDFRSFEHNFEVNAFMYDPSSALLLKGIFLQDQKDAILLQRKTWIKRPWYQKAQESIVRLLAPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=481, Percent_Identity=29.9376299376299, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787007, Length=352, Percent_Identity=28.6931818181818, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87081826, Length=425, Percent_Identity=25.1764705882353, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 55392; Mature: 55392

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEWSIFNEIFAVAFNVLYFVIIIGTIFIVILDNRNPVKTMAWVLVLFFLPIVGLVFYFF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FGRSTRKEKLISKKGFTRLIKRPMAEYQAQKAFKCPDEQHQLMRFFRKVNNALPFEGNAT
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EVFTDGYSMLYSLMKEISKAKHHIHLQFYIFEDDPAGRLLRDLLIDKARQGVEVRLLYDD
EEEECCHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEC
VGCWKVPHAFFDEMRGAGIEARSFLKVRFPLFTSKVNYRNHRKIVVIDGRVAYTGGMNIA
CCCCCCCHHHHHHHCCCCCCHHHEEEEEECCEECCCCCCCCCEEEEECCEEEEECCCHHE
LRYMKGFPWGIWRDTHIKIEGKAVYGLQTAFLTDWYVVDRTLITSSRYFPEVGIFGKALI
EHHHCCCCCCCEECCEEEEECEEEEEEHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHH
QIVTSDPVGEWRDIMQGLLIAISSSRKYFYIQTPYLLPTEPILMALKTAALAGVDVRIMI
HHHCCCCCHHHHHHHHHHHHEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEE
PARADTWITHLGSLSYLDDIMRAGVRIYLYQKGFLHSKLMVSDDTLSTVGSTNMDFRSFE
ECCCHHHHHHHCCHHHHHHHHHCCEEEEEEECCCCCCEEEECCCCHHHCCCCCCCHHHHC
HNFEVNAFMYDPSSALLLKGIFLQDQKDAILLQRKTWIKRPWYQKAQESIVRLLAPLL
CCCEEEEEEECCCCHHHHHHEEECCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVEWSIFNEIFAVAFNVLYFVIIIGTIFIVILDNRNPVKTMAWVLVLFFLPIVGLVFYFF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FGRSTRKEKLISKKGFTRLIKRPMAEYQAQKAFKCPDEQHQLMRFFRKVNNALPFEGNAT
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EVFTDGYSMLYSLMKEISKAKHHIHLQFYIFEDDPAGRLLRDLLIDKARQGVEVRLLYDD
EEEECCHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEC
VGCWKVPHAFFDEMRGAGIEARSFLKVRFPLFTSKVNYRNHRKIVVIDGRVAYTGGMNIA
CCCCCCCHHHHHHHCCCCCCHHHEEEEEECCEECCCCCCCCCEEEEECCEEEEECCCHHE
LRYMKGFPWGIWRDTHIKIEGKAVYGLQTAFLTDWYVVDRTLITSSRYFPEVGIFGKALI
EHHHCCCCCCCEECCEEEEECEEEEEEHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHH
QIVTSDPVGEWRDIMQGLLIAISSSRKYFYIQTPYLLPTEPILMALKTAALAGVDVRIMI
HHHCCCCCHHHHHHHHHHHHEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEE
PARADTWITHLGSLSYLDDIMRAGVRIYLYQKGFLHSKLMVSDDTLSTVGSTNMDFRSFE
ECCCHHHHHHHCCHHHHHHHHHCCEEEEEEECCCCCCEEEECCCCHHHCCCCCCCHHHHC
HNFEVNAFMYDPSSALLLKGIFLQDQKDAILLQRKTWIKRPWYQKAQESIVRLLAPLL
CCCEEEEEEECCCCHHHHHHEEECCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA