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The map label for this gene is fumB [H]

Identifier: 150004412

GI number: 150004412

Start: 2423867

End: 2425510

Strand: Direct

Name: fumB [H]

Synonym: BVU_1859

Alternate gene names: 150004412

Gene position: 2423867-2425510 (Clockwise)

Preceding gene: 150004410

Following gene: 150004413

Centisome position: 46.95

GC content: 44.28

Gene sequence:

>1644_bases
ATGGCAACACCTCCGTTCCACTATCAGCACATGTTCCCGTTGGGACCTGATAAAACCGAGTATTATTTGCTGACTAAAGA
TTATGTGTCAGTAAGTGAGTTTGAAGGAAAACCTATCTTGAAGATTGAAAAAGAAGGTCTGACTGCTATGGCTAACGCTG
CTTTCCGTGATGTTTCTTTCTTGCTTCGCCGTTCACACAATGAACAGGTTGCTAAGATTCTGAGTGATCCCGAAGCCAGT
GATAACGATAAGTATGTAGCATTGACTTTCTTGCGTAATGCGGAAGTTTCAGCTAAAGGCAAATTACCTCTTTGTCAAGA
TACGGGTACTGCTATTATCCATGGTGAGAAAGGTCAGCAGGTATGGACCGGCTATTGTGATGAAGAAGCCCTTTCCTTGG
GAGTTTATAAGACTTATACGGAAGAAAATCTTCGTTATTCTCAGAATGCTCCTTTGACTATGTACGATGAGGTGAACACC
AAATGTAACCTGCCTGCACAAATTGATTTGGAAGCTACTGAAGGTATGGAATACAAGTTCCTTTGTGTGACTAAAGGTGG
TGGTTCGGCGAACAAGACCTATTTATATCAGGAAACGAAAGCTGTATTGAATCCGGCTACATTGGTTCCTTTCCTTGTTG
AAAAAATGAAGACTTTGGGTACGGCTGCTTGTCCTCCTTATCATATTGCATTTGTAATCGGTGGTACATCGGCAGAGAAG
AACTTGCTGACTGTTAAGCTAGCTTCTACTCACTATTATGACGAGTTGCCTACTACCGGTAATGAATACGGGCGTGCTTT
CCGCGATGTGGAACTGGAAAAACAAGTATTGGAAGAAGCTTATAAGATTGGCTTGGGTGCACAGTTTGGTGGTAAGTATA
TGGCTCACGATGTCCGCATCATCCGCTTGCCTCGTCACGGTGCTTCTTGTCCTATCGGTCTGGGCGTTTCCTGTTCTGCT
GACCGTAATATAAAATGTAAGATCAATAAAGATGGTATCTGGATTGAAAAGATGGATGATAAACCGGGTGAGCTGATTCC
GGCAGAACTCCGTGAAGCCGGTGAAGGCGATGTGGTTAAGATTGATTTGAACCAACCGATGGCGGATATCCTGAAAGAAT
TGACCAAGTATCCGGTAGCTACCCGTCTGTCATTGAACGGAACCATTATTGTAGGTCGCGATATCGCTCATGCTAAATTG
AAAGAGCGTTTGGATCGTGGCGAAGATTTGCCGCAGTATATCAAAGATCATCCTATCTATTATGCCGGTCCGGCAAAGAC
TCCGGAAGGTATGGCTTGTGGTTCTATGGGCCCCACTACAGCAGGACGTATGGACCCGTATGTGGATTTGTTCCAGTCAC
ATGGCGGTAGCATGATAATGTTGGCAAAAGGTAATCGTAGCCAGCAGGTAACGGATGCTTGTAAGAAGTATGGCGGTTTC
TATCTGGGCTCTATTGGTGGTCCGGCTGCTATCCTTGCTCAGAATAATATCAAGAGCATTGAGTGTGTGGAATATCCGGA
ATTGGGTATGGAAGCTATCTGGAAAATTGAAGTGGAAGATTTCCCGGCATTCATCTTGGTGGACGATAAAGGTAATGACT
TCTTCAAGCAGTTGAAGCCGCGTTGCCTTGGAAATTGTAAATAA

Upstream 100 bases:

>100_bases
AGGACATTCCTTGAAAGTTTTCACGCTAATTAAGGAAAAATTTCGATAATAGTTACTACCTTTGTCACACTAATAACTTT
TAACTAAAATAAGTATCAAT

Downstream 100 bases:

>100_bases
TTAAATTATTCCGTAATAATACTAATACAGCCTTCCTCCCTTTAAAGGCGAGGGGGCTGTTCTGTTTTTTATTTAATTGG
TGTTATCCGTTACCTTAATA

Product: anaerobic fumarate hydratase class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MATPPFHYQHMFPLGPDKTEYYLLTKDYVSVSEFEGKPILKIEKEGLTAMANAAFRDVSFLLRRSHNEQVAKILSDPEAS
DNDKYVALTFLRNAEVSAKGKLPLCQDTGTAIIHGEKGQQVWTGYCDEEALSLGVYKTYTEENLRYSQNAPLTMYDEVNT
KCNLPAQIDLEATEGMEYKFLCVTKGGGSANKTYLYQETKAVLNPATLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
NLLTVKLASTHYYDELPTTGNEYGRAFRDVELEKQVLEEAYKIGLGAQFGGKYMAHDVRIIRLPRHGASCPIGLGVSCSA
DRNIKCKINKDGIWIEKMDDKPGELIPAELREAGEGDVVKIDLNQPMADILKELTKYPVATRLSLNGTIIVGRDIAHAKL
KERLDRGEDLPQYIKDHPIYYAGPAKTPEGMACGSMGPTTAGRMDPYVDLFQSHGGSMIMLAKGNRSQQVTDACKKYGGF
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQLKPRCLGNCK

Sequences:

>Translated_547_residues
MATPPFHYQHMFPLGPDKTEYYLLTKDYVSVSEFEGKPILKIEKEGLTAMANAAFRDVSFLLRRSHNEQVAKILSDPEAS
DNDKYVALTFLRNAEVSAKGKLPLCQDTGTAIIHGEKGQQVWTGYCDEEALSLGVYKTYTEENLRYSQNAPLTMYDEVNT
KCNLPAQIDLEATEGMEYKFLCVTKGGGSANKTYLYQETKAVLNPATLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
NLLTVKLASTHYYDELPTTGNEYGRAFRDVELEKQVLEEAYKIGLGAQFGGKYMAHDVRIIRLPRHGASCPIGLGVSCSA
DRNIKCKINKDGIWIEKMDDKPGELIPAELREAGEGDVVKIDLNQPMADILKELTKYPVATRLSLNGTIIVGRDIAHAKL
KERLDRGEDLPQYIKDHPIYYAGPAKTPEGMACGSMGPTTAGRMDPYVDLFQSHGGSMIMLAKGNRSQQVTDACKKYGGF
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQLKPRCLGNCK
>Mature_546_residues
ATPPFHYQHMFPLGPDKTEYYLLTKDYVSVSEFEGKPILKIEKEGLTAMANAAFRDVSFLLRRSHNEQVAKILSDPEASD
NDKYVALTFLRNAEVSAKGKLPLCQDTGTAIIHGEKGQQVWTGYCDEEALSLGVYKTYTEENLRYSQNAPLTMYDEVNTK
CNLPAQIDLEATEGMEYKFLCVTKGGGSANKTYLYQETKAVLNPATLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEKN
LLTVKLASTHYYDELPTTGNEYGRAFRDVELEKQVLEEAYKIGLGAQFGGKYMAHDVRIIRLPRHGASCPIGLGVSCSAD
RNIKCKINKDGIWIEKMDDKPGELIPAELREAGEGDVVKIDLNQPMADILKELTKYPVATRLSLNGTIIVGRDIAHAKLK
ERLDRGEDLPQYIKDHPIYYAGPAKTPEGMACGSMGPTTAGRMDPYVDLFQSHGGSMIMLAKGNRSQQVTDACKKYGGFY
LGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQLKPRCLGNCK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=546, Percent_Identity=75.6410256410256, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=547, Percent_Identity=75.6855575868373, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=172, Percent_Identity=29.6511627906977, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60485; Mature: 60354

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPPFHYQHMFPLGPDKTEYYLLTKDYVSVSEFEGKPILKIEKEGLTAMANAAFRDVSF
CCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHH
LLRRSHNEQVAKILSDPEASDNDKYVALTFLRNAEVSAKGKLPLCQDTGTAIIHGEKGQQ
HHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCEECCCCCEEEECCCCCE
VWTGYCDEEALSLGVYKTYTEENLRYSQNAPLTMYDEVNTKCNLPAQIDLEATEGMEYKF
EEECCCCHHHHEECEEEEECCCCCEECCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEE
LCVTKGGGSANKTYLYQETKAVLNPATLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLLTVKLASTHYYDELPTTGNEYGRAFRDVELEKQVLEEAYKIGLGAQFGGKYMAHDVRI
CEEEEEEECCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
IRLPRHGASCPIGLGVSCSADRNIKCKINKDGIWIEKMDDKPGELIPAELREAGEGDVVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEECCCCCCCCCCHHHHCCCCCCEEE
IDLNQPMADILKELTKYPVATRLSLNGTIIVGRDIAHAKLKERLDRGEDLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECCEEEEECCHHHHHHHHHHHCCCCCHHHHHHCCEE
YAGPAKTPEGMACGSMGPTTAGRMDPYVDLFQSHGGSMIMLAKGNRSQQVTDACKKYGGF
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCE
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQLKP
EEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCH
RCLGNCK
HHCCCCC
>Mature Secondary Structure 
ATPPFHYQHMFPLGPDKTEYYLLTKDYVSVSEFEGKPILKIEKEGLTAMANAAFRDVSF
CCCCCCCEEECCCCCCCCEEEEEECCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHH
LLRRSHNEQVAKILSDPEASDNDKYVALTFLRNAEVSAKGKLPLCQDTGTAIIHGEKGQQ
HHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCEECCCCCEEEECCCCCE
VWTGYCDEEALSLGVYKTYTEENLRYSQNAPLTMYDEVNTKCNLPAQIDLEATEGMEYKF
EEECCCCHHHHEECEEEEECCCCCEECCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEE
LCVTKGGGSANKTYLYQETKAVLNPATLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLLTVKLASTHYYDELPTTGNEYGRAFRDVELEKQVLEEAYKIGLGAQFGGKYMAHDVRI
CEEEEEEECCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
IRLPRHGASCPIGLGVSCSADRNIKCKINKDGIWIEKMDDKPGELIPAELREAGEGDVVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEECCCCCCCCCCHHHHCCCCCCEEE
IDLNQPMADILKELTKYPVATRLSLNGTIIVGRDIAHAKLKERLDRGEDLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECCEEEEECCHHHHHHHHHHHCCCCCHHHHHHCCEE
YAGPAKTPEGMACGSMGPTTAGRMDPYVDLFQSHGGSMIMLAKGNRSQQVTDACKKYGGF
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCE
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQLKP
EEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCH
RCLGNCK
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]