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The map label for this gene is holB [C]

Identifier: 150004383

GI number: 150004383

Start: 2375237

End: 2376361

Strand: Reverse

Name: holB [C]

Synonym: BVU_1829

Alternate gene names: 150004383

Gene position: 2376361-2375237 (Counterclockwise)

Preceding gene: 150004384

Following gene: 150004382

Centisome position: 46.03

GC content: 41.96

Gene sequence:

>1125_bases
ATGTTATTTAAAGATGTAATAGGACAAGAGGAAGCAAAACAGCGGCTCATAGCCGAGGTAAAAGAAGGAAGAATCCCTCA
TGCACAACTGATTTGCGGTCCCGAAGGTACAGGAAAGTTGCCTTTGGCAATAGCTTATGCCCGTTACATCTGCTGTGAAA
ACCGTGGTGAACAAGATGCCTGCGGAATTTGCCCCACTTGTGTGAAATTCAATAAGCTGATACATCCCGACCTGCATTTC
GTTTTTCCTGTTATCAAGAAAAAAGCCGGAAAGGATACCGTATGCGATGATTTCATAGCAGACTGGCGCAACTTTGTCCT
GCAGAACCCATATTTTAATCTGAACCATTGGCTGAAAGAGATGGGAGCAGAAAACCAACAGGCGCAAATCTTTGTAAAAG
AGAGCGATGAGATTGTACGAAAGCTCAGCTTAAAATCCAGTCAAGGCGGTTACAAAATCATGATTATATGGCTTCCCGAA
AAGATGAACGTGGAATGCAGCAACAAACTGCTGAAGTTACTGGAAGAGCCTCCTGCCATGACTGTCTTTTTATTAGTATC
CGAAGAGCCTGATGCCATTTTGCAAACGATCCAAAGCCGTACCCAACGATTTAACATTCATGGGATCAAAGAACCTGAAA
TCTCAAAAGTATTGCAAACCAAATACGGACTACAACCGGAAGATGCTGACGATATAGCACACCGCTCAGAAGGCAATTTT
CTGAAAGCACTGGAAACCATACATCTGAGCGAGGAAAACAAACTGTTCTTTGAACTCTTTATAAATTTGATGCGCCTTTC
CTATCAACGGAAGATACGCGAAATGAGACAATGGAGCGATGCGGTAGCCTCCATGGGGCGTGAACGTCAGAAGAATTTTC
TGGCCTACTGCCAACGTATGATACGAGAAAACTTCATATACAACTTTCATCAACGTGATTTAGTGTATATGAATCCCGAA
GAACAAAATTTTTCCACCCGTTTCGCACCGTTTGTCAACGAGCGGAATGTGATGGGCATAATGGACGAACTTAGTGAAGC
ACAATTACATATAGGACAGAATGTGAATCCCAAAATGGTGTTCTTTGATTTCTCTTTAAAAATGATTGTGCTATTGAAAA
ATTAA

Upstream 100 bases:

>100_bases
GGTTCCCAGCATTCATTTCTATACCGTAAGTGCCGTGAACAGTGTAAAGGAAGTTGCAAAAGCAATTTATTAAACAGTAA
ATAAATGACTATAAAAAGCA

Downstream 100 bases:

>100_bases
AAGTTGAGAATTGAGCATTAAAAAATAACAGAACCGCATGGCATTTATTTCTTAGTTCTCAATTTTCAATTCTCAATTAA
AACAGAATTATGGACAATGA

Product: putative DNA polymerase III, delta subunit

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Delta Prime Subunit; DNA-Directed DNA Polymerase; DNA Polymerase III Delta Subunit; DNA Polymerase III Gamma/Tau Subunit; DNA Polymerase III Gamma/Tau Subunits-Like Protein; DNA Polymerase III Gamma And Tau Subunit Protein; DNA Polymerase III Gamma/Tau Subunits; DNA Polymerase III Subunits Gamma And Tau; DNA-Directed DNA Polymerase Delta Prime Subunit; DNA Polymerase III Gamma And Tau Subunit; DNA Polymerase III Delta Prime Subunit Protein; ATPase Involved In DNA Replication; DNA Polymerase III Subunit Gamma/Tau; AAA ATPase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MLFKDVIGQEEAKQRLIAEVKEGRIPHAQLICGPEGTGKLPLAIAYARYICCENRGEQDACGICPTCVKFNKLIHPDLHF
VFPVIKKKAGKDTVCDDFIADWRNFVLQNPYFNLNHWLKEMGAENQQAQIFVKESDEIVRKLSLKSSQGGYKIMIIWLPE
KMNVECSNKLLKLLEEPPAMTVFLLVSEEPDAILQTIQSRTQRFNIHGIKEPEISKVLQTKYGLQPEDADDIAHRSEGNF
LKALETIHLSEENKLFFELFINLMRLSYQRKIREMRQWSDAVASMGRERQKNFLAYCQRMIRENFIYNFHQRDLVYMNPE
EQNFSTRFAPFVNERNVMGIMDELSEAQLHIGQNVNPKMVFFDFSLKMIVLLKN

Sequences:

>Translated_374_residues
MLFKDVIGQEEAKQRLIAEVKEGRIPHAQLICGPEGTGKLPLAIAYARYICCENRGEQDACGICPTCVKFNKLIHPDLHF
VFPVIKKKAGKDTVCDDFIADWRNFVLQNPYFNLNHWLKEMGAENQQAQIFVKESDEIVRKLSLKSSQGGYKIMIIWLPE
KMNVECSNKLLKLLEEPPAMTVFLLVSEEPDAILQTIQSRTQRFNIHGIKEPEISKVLQTKYGLQPEDADDIAHRSEGNF
LKALETIHLSEENKLFFELFINLMRLSYQRKIREMRQWSDAVASMGRERQKNFLAYCQRMIRENFIYNFHQRDLVYMNPE
EQNFSTRFAPFVNERNVMGIMDELSEAQLHIGQNVNPKMVFFDFSLKMIVLLKN
>Mature_374_residues
MLFKDVIGQEEAKQRLIAEVKEGRIPHAQLICGPEGTGKLPLAIAYARYICCENRGEQDACGICPTCVKFNKLIHPDLHF
VFPVIKKKAGKDTVCDDFIADWRNFVLQNPYFNLNHWLKEMGAENQQAQIFVKESDEIVRKLSLKSSQGGYKIMIIWLPE
KMNVECSNKLLKLLEEPPAMTVFLLVSEEPDAILQTIQSRTQRFNIHGIKEPEISKVLQTKYGLQPEDADDIAHRSEGNF
LKALETIHLSEENKLFFELFINLMRLSYQRKIREMRQWSDAVASMGRERQKNFLAYCQRMIRENFIYNFHQRDLVYMNPE
EQNFSTRFAPFVNERNVMGIMDELSEAQLHIGQNVNPKMVFFDFSLKMIVLLKN

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. [C]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 43532; Mature: 43532

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFKDVIGQEEAKQRLIAEVKEGRIPHAQLICGPEGTGKLPLAIAYARYICCENRGEQDA
CCCHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
CGICPTCVKFNKLIHPDLHFVFPVIKKKAGKDTVCDDFIADWRNFVLQNPYFNLNHWLKE
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
MGAENQQAQIFVKESDEIVRKLSLKSSQGGYKIMIIWLPEKMNVECSNKLLKLLEEPPAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCE
TVFLLVSEEPDAILQTIQSRTQRFNIHGIKEPEISKVLQTKYGLQPEDADDIAHRSEGNF
EEEEEEECCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCH
LKALETIHLSEENKLFFELFINLMRLSYQRKIREMRQWSDAVASMGRERQKNFLAYCQRM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRENFIYNFHQRDLVYMNPEEQNFSTRFAPFVNERNVMGIMDELSEAQLHIGQNVNPKMV
HHHHHHEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
FFDFSLKMIVLLKN
EEEECEEEEEEECC
>Mature Secondary Structure
MLFKDVIGQEEAKQRLIAEVKEGRIPHAQLICGPEGTGKLPLAIAYARYICCENRGEQDA
CCCHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
CGICPTCVKFNKLIHPDLHFVFPVIKKKAGKDTVCDDFIADWRNFVLQNPYFNLNHWLKE
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
MGAENQQAQIFVKESDEIVRKLSLKSSQGGYKIMIIWLPEKMNVECSNKLLKLLEEPPAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCE
TVFLLVSEEPDAILQTIQSRTQRFNIHGIKEPEISKVLQTKYGLQPEDADDIAHRSEGNF
EEEEEEECCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCH
LKALETIHLSEENKLFFELFINLMRLSYQRKIREMRQWSDAVASMGRERQKNFLAYCQRM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRENFIYNFHQRDLVYMNPEEQNFSTRFAPFVNERNVMGIMDELSEAQLHIGQNVNPKMV
HHHHHHEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
FFDFSLKMIVLLKN
EEEECEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA