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The map label for this gene is pemA [H]

Identifier: 150004352

GI number: 150004352

Start: 2317305

End: 2318255

Strand: Direct

Name: pemA [H]

Synonym: BVU_1798

Alternate gene names: 150004352

Gene position: 2317305-2318255 (Clockwise)

Preceding gene: 150004351

Following gene: 150004353

Centisome position: 44.88

GC content: 40.9

Gene sequence:

>951_bases
ATGAGGAAAGTTTTAGGATTATTGTTGCTATTGTCTGTTGTAAGCGCAGCATGGGCTCAGGAACGTCAGGATACAATTGT
AGTCTCACGTGATGGAACGGGGAATTTTCGTACTTTGCAGGAGGCTATAGAGAGTGCGCGGGCTTTCATGGATTATACTG
TTACGATCTATGTGAAGAACGGAGTATACAAGGAGAAAGTGATTGTTCCTTCATGGGTGGAAAATATAGATATTATAGGT
GAAGATCGTGATAAGACGATTATTACTTATGATGATCATGCGAATATAAATAAAATGGGTACTTTCCGCACTTATACGGT
GAAGGTAGAGGGCAGTGATATCACTTTTAAAAATTTGACTATTGAGAACAATGCGGCTCAGTTGGGACAGGCGGTAGCTT
TACATACGGAAGGTGACCGTTTGAAATTTATCAATTGTCGTATCTTGGGCAATCAGGACACTATTTATACCGGTGCTAAA
TTTACCCGTCTTTATTTCAAGGATTGTTATATTGATGGTACTACCGACTTTATTTTCGGTCCTTCCACCGCTTTATTTGA
AGATTGTATTATTCACAGTAAACGCAATTCGTATGTAACGGCTGCTTCCACTCCGAAAGAGGCTAAATATGGATATGTCT
TTAAGCACTGTAAGCTGACGGCAGAACCGGGAGTGGATAAAGTGTATTTAGGTCGTCCTTGGCGTCCATATGCTTATACT
TTATTTATAGAATGTGAATTGGGGAAACATATTGTTCTTGCCGGATGGCACAATTGGGGTAAGCAGTCTAATGAAGAAAC
CGCCCGTTATATGGAATATAAAAATACCGGTGAGGGCGCAAATGCTTCGGAAAGGGTGGCGTGGAGCAAACAATTAACAA
AGAAAGAAGCTGAGGCGGTGACGGTCGATGCTATTTTTCGTACTCAAAGTGATTGGAACCCAATAGACTAG

Upstream 100 bases:

>100_bases
CGAAGGCAGTAGGTCCGTTTATTATGGCCAGTCTGGAATGGGAACGTTTGTTTCAGAAAAACAGTTGTGGAACTGCATGT
GAATAATAGGAGGTGATACG

Downstream 100 bases:

>100_bases
ATTGATTTTGTAATTGCTTGAAAAGGAGACTTCTACGTTGGTGGAGGTCTCCTTTTTTTGTTGTACTGAATAATTGGATC
CCGTAAAAAATACCTAATAA

Product: carbohydrate esterase family 8 protein

Products: NA

Alternate protein names: PE A; Pectin methylesterase A [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRKVLGLLLLLSVVSAAWAQERQDTIVVSRDGTGNFRTLQEAIESARAFMDYTVTIYVKNGVYKEKVIVPSWVENIDIIG
EDRDKTIITYDDHANINKMGTFRTYTVKVEGSDITFKNLTIENNAAQLGQAVALHTEGDRLKFINCRILGNQDTIYTGAK
FTRLYFKDCYIDGTTDFIFGPSTALFEDCIIHSKRNSYVTAASTPKEAKYGYVFKHCKLTAEPGVDKVYLGRPWRPYAYT
LFIECELGKHIVLAGWHNWGKQSNEETARYMEYKNTGEGANASERVAWSKQLTKKEAEAVTVDAIFRTQSDWNPID

Sequences:

>Translated_316_residues
MRKVLGLLLLLSVVSAAWAQERQDTIVVSRDGTGNFRTLQEAIESARAFMDYTVTIYVKNGVYKEKVIVPSWVENIDIIG
EDRDKTIITYDDHANINKMGTFRTYTVKVEGSDITFKNLTIENNAAQLGQAVALHTEGDRLKFINCRILGNQDTIYTGAK
FTRLYFKDCYIDGTTDFIFGPSTALFEDCIIHSKRNSYVTAASTPKEAKYGYVFKHCKLTAEPGVDKVYLGRPWRPYAYT
LFIECELGKHIVLAGWHNWGKQSNEETARYMEYKNTGEGANASERVAWSKQLTKKEAEAVTVDAIFRTQSDWNPID
>Mature_316_residues
MRKVLGLLLLLSVVSAAWAQERQDTIVVSRDGTGNFRTLQEAIESARAFMDYTVTIYVKNGVYKEKVIVPSWVENIDIIG
EDRDKTIITYDDHANINKMGTFRTYTVKVEGSDITFKNLTIENNAAQLGQAVALHTEGDRLKFINCRILGNQDTIYTGAK
FTRLYFKDCYIDGTTDFIFGPSTALFEDCIIHSKRNSYVTAASTPKEAKYGYVFKHCKLTAEPGVDKVYLGRPWRPYAYT
LFIECELGKHIVLAGWHNWGKQSNEETARYMEYKNTGEGANASERVAWSKQLTKKEAEAVTVDAIFRTQSDWNPID

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 35856; Mature: 35856

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00503 PECTINESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVLGLLLLLSVVSAAWAQERQDTIVVSRDGTGNFRTLQEAIESARAFMDYTVTIYVKN
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEEEEEEC
GVYKEKVIVPSWVENIDIIGEDRDKTIITYDDHANINKMGTFRTYTVKVEGSDITFKNLT
CCEECEEECCHHHCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEEEECCEEEEEEEE
IENNAAQLGQAVALHTEGDRLKFINCRILGNQDTIYTGAKFTRLYFKDCYIDGTTDFIFG
EECCHHHHCCEEEEEECCCEEEEEEEEEECCCCEEEECCEEEEEEEEEEEECCCCCEEEC
PSTALFEDCIIHSKRNSYVTAASTPKEAKYGYVFKHCKLTAEPGVDKVYLGRPWRPYAYT
CCHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCCEEEE
LFIECELGKHIVLAGWHNWGKQSNEETARYMEYKNTGEGANASERVAWSKQLTKKEAEAV
EEEEEECCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEE
TVDAIFRTQSDWNPID
EEEEEEECCCCCCCCC
>Mature Secondary Structure
MRKVLGLLLLLSVVSAAWAQERQDTIVVSRDGTGNFRTLQEAIESARAFMDYTVTIYVKN
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEEEEEEC
GVYKEKVIVPSWVENIDIIGEDRDKTIITYDDHANINKMGTFRTYTVKVEGSDITFKNLT
CCEECEEECCHHHCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEEEECCEEEEEEEE
IENNAAQLGQAVALHTEGDRLKFINCRILGNQDTIYTGAKFTRLYFKDCYIDGTTDFIFG
EECCHHHHCCEEEEEECCCEEEEEEEEEECCCCEEEECCEEEEEEEEEEEECCCCCEEEC
PSTALFEDCIIHSKRNSYVTAASTPKEAKYGYVFKHCKLTAEPGVDKVYLGRPWRPYAYT
CCHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCCEEEE
LFIECELGKHIVLAGWHNWGKQSNEETARYMEYKNTGEGANASERVAWSKQLTKKEAEAV
EEEEEECCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEE
TVDAIFRTQSDWNPID
EEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370537 [H]