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The map label for this gene is gatC [H]

Identifier: 150004350

GI number: 150004350

Start: 2314380

End: 2315771

Strand: Reverse

Name: gatC [H]

Synonym: BVU_1796

Alternate gene names: 150004350

Gene position: 2315771-2314380 (Counterclockwise)

Preceding gene: 150004366

Following gene: 150004349

Centisome position: 44.85

GC content: 41.16

Gene sequence:

>1392_bases
ATGGAACAAGTTTTTAGTTATATCATCGGTTTAGGAGCAGCGGTGATGATGCCTATAATTTTTACAATCTTAGGCGTTTG
TATCGGAATCAAATTCAGCAAAGCATTAAAAAGCGGTTTGTTGGTAGGTGTTGGTTTCGTTGGTCTATCAGTGGTTACCG
CACTTCTGACCAGCAGTCTGGGTCCGGCCTTAAGCAAAGTGGTCGAAATATATGGCCTGCAACTGAAAGTATTCGATATG
GGATGGCCTGCTGCTGCAGCCGTAGCTTATAACACTTCCGTAGGGGCATTCATCATTCCGGTATGTCTGGCCGTTAACAT
CTTAATGTTATTGACTAAAACCACCCGTACGGTTAATATTGATTTATGGAACTATTGGCATTTCGCTTTTATCGGAGCCG
TAGTTTATTTTGCAACAGATAGTATTTTGTGGGGATTCTTCGCTGCTGTTATCTGTTATATCATCACATTAATCTTAGCA
GATTACACAGCAGACAAATTTCAAGGTTTCTATGATAAAATGGAAGGTATTTCTATTCCGCAGCCCTTCTGTGCAGGTTT
CGTACCATTCGCTCTCATTATAAATAAGGTATTGGACAAAATTCCCGGATTTGAAAAGCTGAACATTGATGCGGAAGGTA
TGAAAAAGAAATTCGGTTTATTAGGCGAACCTCTCTTCTTGGGTATCCTGGTAGGATGTGGCATCGGTGCATTATCTTGC
AAGAACGGACAAGAAATAGTAGACAAGATTCCTTATATTCTAGGTTTAGGTATCAAGATGGGAGCGGTAATGGAGTTGAT
TCCCCGTATTACCAGCCTGTTCATCGAAGGGTTGAAACCCATTTCGGACGCTACCCGCGAACTGATCGCCAAGAAGTTTA
AGGGTGCTGTAGGCTTAAATATCGGTATGAGTCCTGCCTTGGTGATCGGACATCCGGCCACGTTGGTTGTATCATTACTA
TTAATCCCTGTTACTATCTTATTGGCAGTGGTGCTTCCGGGCAATGAATTCTTGCCATTGGCTTCATTAGCTGGCATGTT
CTATGTATTCCCATTGATATTACCTATCACAAAAGGAAATGTAGTAAAGACTTTCATCATTGGTTTTGTTGTGCTGGCCA
TCGGTCTGTATTTTGTAACAGATCTGGCTCCTTATTTCACCAAAGCAGCACATGATGTATACGCCAAGACACAAGATGCT
GCCGTAAACATCCCGTCTGGTTTTGAAGGTGGTGCGTTGGATTTTGCTTCAAGTCCTTTCTCATGGGCTATTTTCCATTT
GACTTATTCATTCAAATGGATTGGTTCCGGAATTTTAGTACTGATAACACTATTCTTAATGGTGCTGAACCGTCGTTCTA
TCATTAAATACCAGAAAACAATGAAAAATTAA

Upstream 100 bases:

>100_bases
TTTCAGGTTTGATAAGTTGAGAAAAGGTTTTATTTTTGGGGCAGTTTTTAATTTATACTATTGTAGAAAACACAGTCATT
TACTATTAATTCACAAGAGA

Downstream 100 bases:

>100_bases
AATTTAAAGATAATGATTATGAAAAAATTAGCGATTGCAATGATGCTGAGTGTTTCTGCCTTGGCAGCTTCAGCTCAAGT
AAATTACAAAGTACAGACCG

Product: putative phosphotransferase enzyme II, C component

Products: galactitol-1-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gat; PTS system galactitol-specific EIIC component [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEQVFSYIIGLGAAVMMPIIFTILGVCIGIKFSKALKSGLLVGVGFVGLSVVTALLTSSLGPALSKVVEIYGLQLKVFDM
GWPAAAAVAYNTSVGAFIIPVCLAVNILMLLTKTTRTVNIDLWNYWHFAFIGAVVYFATDSILWGFFAAVICYIITLILA
DYTADKFQGFYDKMEGISIPQPFCAGFVPFALIINKVLDKIPGFEKLNIDAEGMKKKFGLLGEPLFLGILVGCGIGALSC
KNGQEIVDKIPYILGLGIKMGAVMELIPRITSLFIEGLKPISDATRELIAKKFKGAVGLNIGMSPALVIGHPATLVVSLL
LIPVTILLAVVLPGNEFLPLASLAGMFYVFPLILPITKGNVVKTFIIGFVVLAIGLYFVTDLAPYFTKAAHDVYAKTQDA
AVNIPSGFEGGALDFASSPFSWAIFHLTYSFKWIGSGILVLITLFLMVLNRRSIIKYQKTMKN

Sequences:

>Translated_463_residues
MEQVFSYIIGLGAAVMMPIIFTILGVCIGIKFSKALKSGLLVGVGFVGLSVVTALLTSSLGPALSKVVEIYGLQLKVFDM
GWPAAAAVAYNTSVGAFIIPVCLAVNILMLLTKTTRTVNIDLWNYWHFAFIGAVVYFATDSILWGFFAAVICYIITLILA
DYTADKFQGFYDKMEGISIPQPFCAGFVPFALIINKVLDKIPGFEKLNIDAEGMKKKFGLLGEPLFLGILVGCGIGALSC
KNGQEIVDKIPYILGLGIKMGAVMELIPRITSLFIEGLKPISDATRELIAKKFKGAVGLNIGMSPALVIGHPATLVVSLL
LIPVTILLAVVLPGNEFLPLASLAGMFYVFPLILPITKGNVVKTFIIGFVVLAIGLYFVTDLAPYFTKAAHDVYAKTQDA
AVNIPSGFEGGALDFASSPFSWAIFHLTYSFKWIGSGILVLITLFLMVLNRRSIIKYQKTMKN
>Mature_463_residues
MEQVFSYIIGLGAAVMMPIIFTILGVCIGIKFSKALKSGLLVGVGFVGLSVVTALLTSSLGPALSKVVEIYGLQLKVFDM
GWPAAAAVAYNTSVGAFIIPVCLAVNILMLLTKTTRTVNIDLWNYWHFAFIGAVVYFATDSILWGFFAAVICYIITLILA
DYTADKFQGFYDKMEGISIPQPFCAGFVPFALIINKVLDKIPGFEKLNIDAEGMKKKFGLLGEPLFLGILVGCGIGALSC
KNGQEIVDKIPYILGLGIKMGAVMELIPRITSLFIEGLKPISDATRELIAKKFKGAVGLNIGMSPALVIGHPATLVVSLL
LIPVTILLAVVLPGNEFLPLASLAGMFYVFPLILPITKGNVVKTFIIGFVVLAIGLYFVTDLAPYFTKAAHDVYAKTQDA
AVNIPSGFEGGALDFASSPFSWAIFHLTYSFKWIGSGILVLITLFLMVLNRRSIIKYQKTMKN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3775

COG function: function code G; Phosphotransferase system, galactitol-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788408, Length=461, Percent_Identity=33.6225596529284, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1790758, Length=393, Percent_Identity=38.676844783715, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013853
- InterPro:   IPR013014
- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: NA

Molecular weight: Translated: 49972; Mature: 49972

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVFSYIIGLGAAVMMPIIFTILGVCIGIKFSKALKSGLLVGVGFVGLSVVTALLTSSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GPALSKVVEIYGLQLKVFDMGWPAAAAVAYNTSVGAFIIPVCLAVNILMLLTKTTRTVNI
CHHHHHHHHHHCCEEEEEECCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
DLWNYWHFAFIGAVVYFATDSILWGFFAAVICYIITLILADYTADKFQGFYDKMEGISIP
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
QPFCAGFVPFALIINKVLDKIPGFEKLNIDAEGMKKKFGLLGEPLFLGILVGCGIGALSC
CHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
KNGQEIVDKIPYILGLGIKMGAVMELIPRITSLFIEGLKPISDATRELIAKKFKGAVGLN
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECC
IGMSPALVIGHPATLVVSLLLIPVTILLAVVLPGNEFLPLASLAGMFYVFPLILPITKGN
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VVKTFIIGFVVLAIGLYFVTDLAPYFTKAAHDVYAKTQDAAVNIPSGFEGGALDFASSPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
SWAIFHLTYSFKWIGSGILVLITLFLMVLNRRSIIKYQKTMKN
CEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MEQVFSYIIGLGAAVMMPIIFTILGVCIGIKFSKALKSGLLVGVGFVGLSVVTALLTSSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GPALSKVVEIYGLQLKVFDMGWPAAAAVAYNTSVGAFIIPVCLAVNILMLLTKTTRTVNI
CHHHHHHHHHHCCEEEEEECCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
DLWNYWHFAFIGAVVYFATDSILWGFFAAVICYIITLILADYTADKFQGFYDKMEGISIP
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
QPFCAGFVPFALIINKVLDKIPGFEKLNIDAEGMKKKFGLLGEPLFLGILVGCGIGALSC
CHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
KNGQEIVDKIPYILGLGIKMGAVMELIPRITSLFIEGLKPISDATRELIAKKFKGAVGLN
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECC
IGMSPALVIGHPATLVVSLLLIPVTILLAVVLPGNEFLPLASLAGMFYVFPLILPITKGN
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VVKTFIIGFVVLAIGLYFVTDLAPYFTKAAHDVYAKTQDAAVNIPSGFEGGALDFASSPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
SWAIFHLTYSFKWIGSGILVLITLFLMVLNRRSIIKYQKTMKN
CEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; galactitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + galactitol [Periplasm] = galactitol-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]