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The map label for this gene is pgl [H]

Identifier: 150004323

GI number: 150004323

Start: 2270654

End: 2272438

Strand: Reverse

Name: pgl [H]

Synonym: BVU_1769

Alternate gene names: 150004323

Gene position: 2272438-2270654 (Counterclockwise)

Preceding gene: 150004325

Following gene: 150004322

Centisome position: 44.01

GC content: 44.76

Gene sequence:

>1785_bases
ATGACAAGAAACGCTTTTTGGGGACTGTGTATCGGGATATGTATGGCAAGCACACAAAGTGTCGCTCAAAAGATAGAAAA
TACAAATATCCAAACTGATAATCAAAAAAACTATACCCCATCCCATATCAATGAAATTACCAATGGGGAATCTTTATATA
CTGTCCGAAGCGACACAAGTCTTCATAACATCAGAACAATACAAGCAAACTCGAATCCGGAAGTATTGGATAAACTGACA
GCAGAACTGAAACCGGCTATCCCCTATTTAAATGAAGTCGGTGCACAAGCCATGCCCCGGAATATCGTTCCGATCAAGAA
TCCCTATTTCAATATGCCTCAGCTGAAGCGTCCCGTTTTCCCTGATTTCATCGTCAATATGAAAGACAAAGGAATGACAG
AAGATGCCCCTATTACAGATTTAGTGAACCGGACGATTGCCGAAGTCAGCAAACAAGGAGGAGGAACAGTCGTCATTCCT
GAGGGAAAATGGAAATCGGCCCGTATTGTACTGAAAAGTAATGTCAATCTGCATTTAGCCAAAGGAGCCGAACTAGAGTT
TTCCGGTTGCGCGGAAGATTATCTGCCGGCGGTTTTCACACGCCATGAAGGGGTAGAAATAATGGGGCCGGCCGCCTTTA
TTTACGCCAATGGGGAAAACAACATCGCCATTACGGGAGAAGGAACCATCTACGGACCACCTATGGATGCTGAAATCAGA
AAACGTCCTAACGGTGCTTCAGTGGTAGAAAAAGATGTTCCGTGGGACATGCCTATAGAACAACGTATCTATGACGGTAT
GGAAGGCAGGACTTTCTATCGTCCTAAAACAATCTCTCCCATCAACTGTACCAATGTATTAATAGAAGGGATCACGATGG
AACGAAGTACCTTGTGGAATGTGGTACCCATTTATTGCGAGAATGTAATTATAAGAGGCATTACCGTCAACTCCACCAAG
GTGCCCAGCGGAGACGGCATTGACATAGAATCCTGTAAAAATGTACTGATTGAATACTGCACGCTGAACTGTGGTGATGA
TTGCTTTACCCTGAAAGCCGGTAGAGCAGAAGACGGATTACGTGTAGGCAAACCTACAGAAAATGTAGTTATCCGCTACT
CGCTGGCCCAGCATGGACATGGCGGGATAACTTGTGGTAGTGAAACAGCCGGAGTGATCAAGAATCTGTATGTACACGAT
TGTGTATTCGACGGAACCCGTACCGGCATCCGTTTCAAGACACGACGCAACCGTGGAGGAGGCAGTGACAACACGTACTA
TGAGAGACTGCGCATGATCAATGTAGGAAAAGCCTTTACCTGGGATTTGTTGGGCAGTGCTTATTATATGGGCGAACTGG
CCGCACGCTATCCGGCAAGAAAAGTAAACCGGCTGACGCCTGATGTAAAGAATATCCTGATCAAAGATTTCATTGTAGAA
TCAGCCGACCAGTTCTTTACCGCCAATGGTATTCCCGAAATACCTTTCAACCAGGTAGTGGTGGAAAATGGAGAGATCAA
ATGCAGGAAATTAATCGGTGCCCTGAATGATGCCGCCGGATTTACGATGCGGAAGCTGACGATAGAATCGCAGCACAATG
ACATTCACATATTGGATGGAAAAGACATCCTGTTCGAGGATATACATTTCAAACTCCCCGCCGGAGAGATCATGGTGAAT
GTGGAAGGGGAAAGATCCGGAAACATTGTTTTCAAGAACATCAATGCCAATCAGGAAAAGGTTGAGTATAAAAAGGAATC
TCCCATGAGGATAGAAATAAAGTAA

Upstream 100 bases:

>100_bases
AAAAATAAGAACAAATCCAATGGTTACAAATTCGATCTATAAACGTCATTACCTATAAGAGCCGCAAAATGAAGGCCATT
TAACTTTTAAAAGACTAAGT

Downstream 100 bases:

>100_bases
CTAAATATCGGTCTGATTTGGAACGCAAATGACGCAAATGACACAAATTTAACCTTTCGGTAGGTTTTGCCTCTATGTAG
TGAGGGGGCTTTATTTGTGT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MTRNAFWGLCIGICMASTQSVAQKIENTNIQTDNQKNYTPSHINEITNGESLYTVRSDTSLHNIRTIQANSNPEVLDKLT
AELKPAIPYLNEVGAQAMPRNIVPIKNPYFNMPQLKRPVFPDFIVNMKDKGMTEDAPITDLVNRTIAEVSKQGGGTVVIP
EGKWKSARIVLKSNVNLHLAKGAELEFSGCAEDYLPAVFTRHEGVEIMGPAAFIYANGENNIAITGEGTIYGPPMDAEIR
KRPNGASVVEKDVPWDMPIEQRIYDGMEGRTFYRPKTISPINCTNVLIEGITMERSTLWNVVPIYCENVIIRGITVNSTK
VPSGDGIDIESCKNVLIEYCTLNCGDDCFTLKAGRAEDGLRVGKPTENVVIRYSLAQHGHGGITCGSETAGVIKNLYVHD
CVFDGTRTGIRFKTRRNRGGGSDNTYYERLRMINVGKAFTWDLLGSAYYMGELAARYPARKVNRLTPDVKNILIKDFIVE
SADQFFTANGIPEIPFNQVVVENGEIKCRKLIGALNDAAGFTMRKLTIESQHNDIHILDGKDILFEDIHFKLPAGEIMVN
VEGERSGNIVFKNINANQEKVEYKKESPMRIEIK

Sequences:

>Translated_594_residues
MTRNAFWGLCIGICMASTQSVAQKIENTNIQTDNQKNYTPSHINEITNGESLYTVRSDTSLHNIRTIQANSNPEVLDKLT
AELKPAIPYLNEVGAQAMPRNIVPIKNPYFNMPQLKRPVFPDFIVNMKDKGMTEDAPITDLVNRTIAEVSKQGGGTVVIP
EGKWKSARIVLKSNVNLHLAKGAELEFSGCAEDYLPAVFTRHEGVEIMGPAAFIYANGENNIAITGEGTIYGPPMDAEIR
KRPNGASVVEKDVPWDMPIEQRIYDGMEGRTFYRPKTISPINCTNVLIEGITMERSTLWNVVPIYCENVIIRGITVNSTK
VPSGDGIDIESCKNVLIEYCTLNCGDDCFTLKAGRAEDGLRVGKPTENVVIRYSLAQHGHGGITCGSETAGVIKNLYVHD
CVFDGTRTGIRFKTRRNRGGGSDNTYYERLRMINVGKAFTWDLLGSAYYMGELAARYPARKVNRLTPDVKNILIKDFIVE
SADQFFTANGIPEIPFNQVVVENGEIKCRKLIGALNDAAGFTMRKLTIESQHNDIHILDGKDILFEDIHFKLPAGEIMVN
VEGERSGNIVFKNINANQEKVEYKKESPMRIEIK
>Mature_593_residues
TRNAFWGLCIGICMASTQSVAQKIENTNIQTDNQKNYTPSHINEITNGESLYTVRSDTSLHNIRTIQANSNPEVLDKLTA
ELKPAIPYLNEVGAQAMPRNIVPIKNPYFNMPQLKRPVFPDFIVNMKDKGMTEDAPITDLVNRTIAEVSKQGGGTVVIPE
GKWKSARIVLKSNVNLHLAKGAELEFSGCAEDYLPAVFTRHEGVEIMGPAAFIYANGENNIAITGEGTIYGPPMDAEIRK
RPNGASVVEKDVPWDMPIEQRIYDGMEGRTFYRPKTISPINCTNVLIEGITMERSTLWNVVPIYCENVIIRGITVNSTKV
PSGDGIDIESCKNVLIEYCTLNCGDDCFTLKAGRAEDGLRVGKPTENVVIRYSLAQHGHGGITCGSETAGVIKNLYVHDC
VFDGTRTGIRFKTRRNRGGGSDNTYYERLRMINVGKAFTWDLLGSAYYMGELAARYPARKVNRLTPDVKNILIKDFIVES
ADQFFTANGIPEIPFNQVVVENGEIKCRKLIGALNDAAGFTMRKLTIESQHNDIHILDGKDILFEDIHFKLPAGEIMVNV
EGERSGNIVFKNINANQEKVEYKKESPMRIEIK

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 66065; Mature: 65934

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNAFWGLCIGICMASTQSVAQKIENTNIQTDNQKNYTPSHINEITNGESLYTVRSDTS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCC
LHNIRTIQANSNPEVLDKLTAELKPAIPYLNEVGAQAMPRNIVPIKNPYFNMPQLKRPVF
CEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCHHHCCCCCCCCCCCCCCCCHHCCCCC
PDFIVNMKDKGMTEDAPITDLVNRTIAEVSKQGGGTVVIPEGKWKSARIVLKSNVNLHLA
CHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCEEEEE
KGAELEFSGCAEDYLPAVFTRHEGVEIMGPAAFIYANGENNIAITGEGTIYGPPMDAEIR
CCCCEEECCCCHHHHHHHHCCCCCCEEECCEEEEEECCCCCEEEEECCEEECCCCCHHHH
KRPNGASVVEKDVPWDMPIEQRIYDGMEGRTFYRPKTISPINCTNVLIEGITMERSTLWN
CCCCCCCEEECCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHHEECEEECCHHHCC
VVPIYCENVIIRGITVNSTKVPSGDGIDIESCKNVLIEYCTLNCGDDCFTLKAGRAEDGL
CHHEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
RVGKPTENVVIRYSLAQHGHGGITCGSETAGVIKNLYVHDCVFDGTRTGIRFKTRRNRGG
CCCCCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHEEECCCCCCEEEEEECCCCC
GSDNTYYERLRMINVGKAFTWDLLGSAYYMGELAARYPARKVNRLTPDVKNILIKDFIVE
CCCCHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHH
SADQFFTANGIPEIPFNQVVVENGEIKCRKLIGALNDAAGFTMRKLTIESQHNDIHILDG
HHHHEEECCCCCCCCCCEEEEECCCEEHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECC
KDILFEDIHFKLPAGEIMVNVEGERSGNIVFKNINANQEKVEYKKESPMRIEIK
CCEEEECEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TRNAFWGLCIGICMASTQSVAQKIENTNIQTDNQKNYTPSHINEITNGESLYTVRSDTS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCC
LHNIRTIQANSNPEVLDKLTAELKPAIPYLNEVGAQAMPRNIVPIKNPYFNMPQLKRPVF
CEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCHHHCCCCCCCCCCCCCCCCHHCCCCC
PDFIVNMKDKGMTEDAPITDLVNRTIAEVSKQGGGTVVIPEGKWKSARIVLKSNVNLHLA
CHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCEEEEE
KGAELEFSGCAEDYLPAVFTRHEGVEIMGPAAFIYANGENNIAITGEGTIYGPPMDAEIR
CCCCEEECCCCHHHHHHHHCCCCCCEEECCEEEEEECCCCCEEEEECCEEECCCCCHHHH
KRPNGASVVEKDVPWDMPIEQRIYDGMEGRTFYRPKTISPINCTNVLIEGITMERSTLWN
CCCCCCCEEECCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHHEECEEECCHHHCC
VVPIYCENVIIRGITVNSTKVPSGDGIDIESCKNVLIEYCTLNCGDDCFTLKAGRAEDGL
CHHEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
RVGKPTENVVIRYSLAQHGHGGITCGSETAGVIKNLYVHDCVFDGTRTGIRFKTRRNRGG
CCCCCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHEEECCCCCCEEEEEECCCCC
GSDNTYYERLRMINVGKAFTWDLLGSAYYMGELAARYPARKVNRLTPDVKNILIKDFIVE
CCCCHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHH
SADQFFTANGIPEIPFNQVVVENGEIKCRKLIGALNDAAGFTMRKLTIESQHNDIHILDG
HHHHEEECCCCCCCCCCEEEEECCCEEHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECC
KDILFEDIHFKLPAGEIMVNVEGERSGNIVFKNINANQEKVEYKKESPMRIEIK
CCEEEECEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]