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The map label for this gene is pgl [H]

Identifier: 150004301

GI number: 150004301

Start: 2243173

End: 2244579

Strand: Reverse

Name: pgl [H]

Synonym: BVU_1747

Alternate gene names: 150004301

Gene position: 2244579-2243173 (Counterclockwise)

Preceding gene: 150004303

Following gene: 150004299

Centisome position: 43.47

GC content: 39.66

Gene sequence:

>1407_bases
ATGGTAAAAAAGTTAGTTAGTGTACTTTGTATATGTGCATATTTTGGACTTGCTCATGCACAAATACCACAAGAGATTTG
GAAAGAAAGTGAGCAAATAGAAAAACAAATCAAAAAGACTTCTTTTCCCGATCGTGTGTACAACATTAAAGATTTCGGTG
CCAAAGAAGGAAATAATGGTGAAATACTCTGCCATGAAGCAATCAATCTAGCTATCCTCACATGCAGCCAGACAGGTGGA
GGAACTGTATTAGTTCCACCCGGAGAATTTCTAACAGGTCCTATCACACTGAAAAGTAATGTAAATCTGCATTTGGAAGA
AGGTGCCTATCTAAAATTTTCTTCAGAGAAATATCTATATACTCCAACCGTACTCACTCGCTGGGAAGGAGTAGACTGTT
ACAATTTGCATCCATTAATTTATGCTTATGGTGAAAGCAACATAGGCATTACCGGTAAAGGTATTATAGACGGACAGGCC
AGTAATGATAACTGGTGGAGTATGTGTGGTGCTCCACACTATGGCTGGAAGGAAGGTATGACAGCTCAAAAAAATGGCGG
CAGAGACAAATTACTGATGTACGCCGAAACATTTGCCCCGATAGATAAGCGCCAAATGACTTTTGAAGACGGTTTACGTC
CCCAACTTATCAATCTATATCGTTGCAACACTATTTTAATAGAAAATGTAACATTGAAAAATTCTCCGTTTTGGGTTATC
CATCCTTTATTTTGTGAAAGTCTGACAGTGCGTGGGGTTAAAGTTTCCAGTCACGGTCCCAATAGCGATGGCTGCGATCC
GGAATCAAGTAAGAACGTATTAATTGAAAACTGTATATTCGATACTGGAGACGACTGCATTGCCATTAAAAGCGGACGGA
ATGCCGACGGACGGAAATGGAATGTACCAAGTGAAAATATCATTGTACGCAATTGCGAAATGAAAGACGGACACGGTGGT
GTAGTTGTAGGCAGTGAGATTTCGGGAGGATATAAGAACCTGTTTGTTGAAAACTGCAAAATGGACAGCCCGAACCTGGA
ACGTGTCATCCGCATCAAAACCAACAACTGCCGTGGTGGTGTCATTGAAAATATTTATGTGCGTAATGTAGAAGTAGGTG
AATGTCGCGAAGCCGTTTTGAAAATCAACCTGCAATATGAAAACCGTGAAAAATGTGACAGAAATTTCCCACCTGTAGTA
CGCCATGTATATCTGGATAATGTTACCAGTGAAAAAAGTAAGTACGGAGTCTTGATTACCGGATATGACGACCGTGTCAA
TATTGAAGACATCCATGTAACAAACAGCCGTTTCAATAATGTAGAGAAAAAGGGAAATTTAATTACCGGAGCTAAGGATG
TAGTACTGAAAGAACTGTATATCAATGGAAATAAGGTAAGAAAATAA

Upstream 100 bases:

>100_bases
TGGGCAATTAATCCACTTATTTTCTAATTATATGCTTTAATATTGCATCGTAGATATTTTTTGGTAACTATGAAATTATT
AATTATTAAACATCAAAGAT

Downstream 100 bases:

>100_bases
GATAAAAAATCTTTGGTATAATGAGATGCCATCATACCAAAGATTATATCCTCTTTTGAGAACCTATTCCAGACACTCAT
CAAGTTCACGTATCAACTCT

Product: polygalacturonase

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVKKLVSVLCICAYFGLAHAQIPQEIWKESEQIEKQIKKTSFPDRVYNIKDFGAKEGNNGEILCHEAINLAILTCSQTGG
GTVLVPPGEFLTGPITLKSNVNLHLEEGAYLKFSSEKYLYTPTVLTRWEGVDCYNLHPLIYAYGESNIGITGKGIIDGQA
SNDNWWSMCGAPHYGWKEGMTAQKNGGRDKLLMYAETFAPIDKRQMTFEDGLRPQLINLYRCNTILIENVTLKNSPFWVI
HPLFCESLTVRGVKVSSHGPNSDGCDPESSKNVLIENCIFDTGDDCIAIKSGRNADGRKWNVPSENIIVRNCEMKDGHGG
VVVGSEISGGYKNLFVENCKMDSPNLERVIRIKTNNCRGGVIENIYVRNVEVGECREAVLKINLQYENREKCDRNFPPVV
RHVYLDNVTSEKSKYGVLITGYDDRVNIEDIHVTNSRFNNVEKKGNLITGAKDVVLKELYINGNKVRK

Sequences:

>Translated_468_residues
MVKKLVSVLCICAYFGLAHAQIPQEIWKESEQIEKQIKKTSFPDRVYNIKDFGAKEGNNGEILCHEAINLAILTCSQTGG
GTVLVPPGEFLTGPITLKSNVNLHLEEGAYLKFSSEKYLYTPTVLTRWEGVDCYNLHPLIYAYGESNIGITGKGIIDGQA
SNDNWWSMCGAPHYGWKEGMTAQKNGGRDKLLMYAETFAPIDKRQMTFEDGLRPQLINLYRCNTILIENVTLKNSPFWVI
HPLFCESLTVRGVKVSSHGPNSDGCDPESSKNVLIENCIFDTGDDCIAIKSGRNADGRKWNVPSENIIVRNCEMKDGHGG
VVVGSEISGGYKNLFVENCKMDSPNLERVIRIKTNNCRGGVIENIYVRNVEVGECREAVLKINLQYENREKCDRNFPPVV
RHVYLDNVTSEKSKYGVLITGYDDRVNIEDIHVTNSRFNNVEKKGNLITGAKDVVLKELYINGNKVRK
>Mature_468_residues
MVKKLVSVLCICAYFGLAHAQIPQEIWKESEQIEKQIKKTSFPDRVYNIKDFGAKEGNNGEILCHEAINLAILTCSQTGG
GTVLVPPGEFLTGPITLKSNVNLHLEEGAYLKFSSEKYLYTPTVLTRWEGVDCYNLHPLIYAYGESNIGITGKGIIDGQA
SNDNWWSMCGAPHYGWKEGMTAQKNGGRDKLLMYAETFAPIDKRQMTFEDGLRPQLINLYRCNTILIENVTLKNSPFWVI
HPLFCESLTVRGVKVSSHGPNSDGCDPESSKNVLIENCIFDTGDDCIAIKSGRNADGRKWNVPSENIIVRNCEMKDGHGG
VVVGSEISGGYKNLFVENCKMDSPNLERVIRIKTNNCRGGVIENIYVRNVEVGECREAVLKINLQYENREKCDRNFPPVV
RHVYLDNVTSEKSKYGVLITGYDDRVNIEDIHVTNSRFNNVEKKGNLITGAKDVVLKELYINGNKVRK

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 52443; Mature: 52443

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKLVSVLCICAYFGLAHAQIPQEIWKESEQIEKQIKKTSFPDRVYNIKDFGAKEGNNG
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEECHHCCCCCCCCC
EILCHEAINLAILTCSQTGGGTVLVPPGEFLTGPITLKSNVNLHLEEGAYLKFSSEKYLY
CEEEEECCEEEEEEEECCCCCEEEECCCCCEECCEEEECCCEEEEECCEEEEECCCCEEE
TPTVLTRWEGVDCYNLHPLIYAYGESNIGITGKGIIDGQASNDNWWSMCGAPHYGWKEGM
CCHHEEEECCCCEEECCEEEEEECCCCCCEEECEEECCCCCCCCCEEECCCCCCCHHCCC
TAQKNGGRDKLLMYAETFAPIDKRQMTFEDGLRPQLINLYRCNTILIENVTLKNSPFWVI
CCCCCCCCCEEEEEEECCCCCCCCCCCHHCCCCCCEEEEEECCEEEEEEEEECCCCEEEE
HPLFCESLTVRGVKVSSHGPNSDGCDPESSKNVLIENCIFDTGDDCIAIKSGRNADGRKW
EHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCEE
NVPSENIIVRNCEMKDGHGGVVVGSEISGGYKNLFVENCKMDSPNLERVIRIKTNNCRGG
CCCCCCEEEEEEECCCCCCCEEECCCCCCCHHEEEEEECCCCCCCCEEEEEEEECCCCCC
VIENIYVRNVEVGECREAVLKINLQYENREKCDRNFPPVVRHVYLDNVTSEKSKYGVLIT
CEEEEEEECCCHHHHCEEEEEEEEEECCHHHHCCCCCHHHHHEEECCCCCCCCCCEEEEE
GYDDRVNIEDIHVTNSRFNNVEKKGNLITGAKDVVLKELYINGNKVRK
CCCCCCCEEEEEEECCHHCCHHHCCCEEECHHHHEEEEEEECCCCCCC
>Mature Secondary Structure
MVKKLVSVLCICAYFGLAHAQIPQEIWKESEQIEKQIKKTSFPDRVYNIKDFGAKEGNNG
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEECHHCCCCCCCCC
EILCHEAINLAILTCSQTGGGTVLVPPGEFLTGPITLKSNVNLHLEEGAYLKFSSEKYLY
CEEEEECCEEEEEEEECCCCCEEEECCCCCEECCEEEECCCEEEEECCEEEEECCCCEEE
TPTVLTRWEGVDCYNLHPLIYAYGESNIGITGKGIIDGQASNDNWWSMCGAPHYGWKEGM
CCHHEEEECCCCEEECCEEEEEECCCCCCEEECEEECCCCCCCCCEEECCCCCCCHHCCC
TAQKNGGRDKLLMYAETFAPIDKRQMTFEDGLRPQLINLYRCNTILIENVTLKNSPFWVI
CCCCCCCCCEEEEEEECCCCCCCCCCCHHCCCCCCEEEEEECCEEEEEEEEECCCCEEEE
HPLFCESLTVRGVKVSSHGPNSDGCDPESSKNVLIENCIFDTGDDCIAIKSGRNADGRKW
EHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCEE
NVPSENIIVRNCEMKDGHGGVVVGSEISGGYKNLFVENCKMDSPNLERVIRIKTNNCRGG
CCCCCCEEEEEEECCCCCCCEEECCCCCCCHHEEEEEECCCCCCCCEEEEEEEECCCCCC
VIENIYVRNVEVGECREAVLKINLQYENREKCDRNFPPVVRHVYLDNVTSEKSKYGVLIT
CEEEEEEECCCHHHHCEEEEEEEEEECCHHHHCCCCCHHHHHEEECCCCCCCCCCEEEEE
GYDDRVNIEDIHVTNSRFNNVEKKGNLITGAKDVVLKELYINGNKVRK
CCCCCCCEEEEEEECCHHCCHHHCCCEEECHHHHEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]