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The map label for this gene is mdtE [H]

Identifier: 150004232

GI number: 150004232

Start: 2160574

End: 2161689

Strand: Direct

Name: mdtE [H]

Synonym: BVU_1673

Alternate gene names: 150004232

Gene position: 2160574-2161689 (Clockwise)

Preceding gene: 150004230

Following gene: 150004233

Centisome position: 41.85

GC content: 45.61

Gene sequence:

>1116_bases
ATGATGACATTGAACAAAAAATGGATGCGACTAATGGTATTGGTTGGTTGTGCTGCTTTGACAGCGTCGTGTAAACAGGC
GCCTGTTGCACAAATGGAGGCGGAATATGCGGTATTAAAGGTATCACCGTCGGACAAAGTTTTGTCTACTACTTATTCGG
CTACGATCCGTGGCCGCCAGGATATTGATATTTATCCGCAAGTTTCCGGTTTCCTTACCCGGCTTTGTGTGGAGGAAGGA
CAGGCAGTGCGTAAAGGGCAAGTCTTATTTATTATTGACCAAGTGCCTTATAAGGCGGCATTGGAAACTGCTGTCGCCAA
TGTGGAATCGGCTAAAGCCGGTCTTGCCACAGCCGAACTGACTTATAACAGCAAGAAAGAATTGTTTGCCAAAAAGGTGG
TTTCAGAGTTTGATCTGAAGACTGCGGAAAACTCTTACCTGACCGCTAAGGCTGGATTGGCTCAGGCTAAGGCGCAGGAA
GTCAGTGCTCGCAATAACCTTTCATATACCGAGGTGAAAAGTCCTTCGGATGGGGTGATTGGTACATTGCCTTATCGGGT
AGGTGCATTGGTTAGTGCCAGTTTGCCACAACCGTTGACTACGGTTTCTGATAATTCGGATATGTATGTCTACTTTTCGA
TGACTGAAAATCAATTGTTGGCTCTGACCCGTCAATATGGTTCCAAAGCAGAAGCGTTGAAAAATATGCCGGAAGTGGAA
CTGCAATTGAATGACAAGTCTATGTATGGCGAAAAGGGACGCATTGAAACCATCAGTGGGGTGGTAGACCGTAATACGGG
TACCGCAAGTCTGCGTGCCGTATTCCCGAATAAGAATGGGTTGCTGTATAGCGGAACATCGGGAAATGTGATTCTTCCGG
TTACGATGAAAGGAAGTTTGGTTATTCCTCAGGCTACAACTTTTGAAATTCAGGATATTACATACGTATATAAAGTCGTG
GACGGCAAGGCACAATCAGCTCCTGTCAGTGTGACCCGTGTAAATGGAGGACAGGAATATATCGTGAACGATGGATTGAA
AGAGGGGGATATCATTGTAGCGGAAGGCGTAGGTTTGCTGCGTGAAGGTACTCCCATTAAAGTAAAACAGAACTAA

Upstream 100 bases:

>100_bases
AGGAAATCTATTCCGAATTTGAACAATGGTAGCTAATAATTGAATGTGTCGGCTTGAAAAATTCCATCTACCTTTGCGTC
CGAATTTAAAAGATTGGAAT

Downstream 100 bases:

>100_bases
GAAAGACTATGAATTTAAGAACCTTTATAGAACGCCCCATCCTGTCGGCTGTTATTTCTATTTCGATAGTGGTAGTGGGT
ATCATCGGGCTGTTCACTTT

Product: multidrug efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MMTLNKKWMRLMVLVGCAALTASCKQAPVAQMEAEYAVLKVSPSDKVLSTTYSATIRGRQDIDIYPQVSGFLTRLCVEEG
QAVRKGQVLFIIDQVPYKAALETAVANVESAKAGLATAELTYNSKKELFAKKVVSEFDLKTAENSYLTAKAGLAQAKAQE
VSARNNLSYTEVKSPSDGVIGTLPYRVGALVSASLPQPLTTVSDNSDMYVYFSMTENQLLALTRQYGSKAEALKNMPEVE
LQLNDKSMYGEKGRIETISGVVDRNTGTASLRAVFPNKNGLLYSGTSGNVILPVTMKGSLVIPQATTFEIQDITYVYKVV
DGKAQSAPVSVTRVNGGQEYIVNDGLKEGDIIVAEGVGLLREGTPIKVKQN

Sequences:

>Translated_371_residues
MMTLNKKWMRLMVLVGCAALTASCKQAPVAQMEAEYAVLKVSPSDKVLSTTYSATIRGRQDIDIYPQVSGFLTRLCVEEG
QAVRKGQVLFIIDQVPYKAALETAVANVESAKAGLATAELTYNSKKELFAKKVVSEFDLKTAENSYLTAKAGLAQAKAQE
VSARNNLSYTEVKSPSDGVIGTLPYRVGALVSASLPQPLTTVSDNSDMYVYFSMTENQLLALTRQYGSKAEALKNMPEVE
LQLNDKSMYGEKGRIETISGVVDRNTGTASLRAVFPNKNGLLYSGTSGNVILPVTMKGSLVIPQATTFEIQDITYVYKVV
DGKAQSAPVSVTRVNGGQEYIVNDGLKEGDIIVAEGVGLLREGTPIKVKQN
>Mature_371_residues
MMTLNKKWMRLMVLVGCAALTASCKQAPVAQMEAEYAVLKVSPSDKVLSTTYSATIRGRQDIDIYPQVSGFLTRLCVEEG
QAVRKGQVLFIIDQVPYKAALETAVANVESAKAGLATAELTYNSKKELFAKKVVSEFDLKTAENSYLTAKAGLAQAKAQE
VSARNNLSYTEVKSPSDGVIGTLPYRVGALVSASLPQPLTTVSDNSDMYVYFSMTENQLLALTRQYGSKAEALKNMPEVE
LQLNDKSMYGEKGRIETISGVVDRNTGTASLRAVFPNKNGLLYSGTSGNVILPVTMKGSLVIPQATTFEIQDITYVYKVV
DGKAQSAPVSVTRVNGGQEYIVNDGLKEGDIIVAEGVGLLREGTPIKVKQN

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=371, Percent_Identity=29.6495956873315, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786668, Length=314, Percent_Identity=28.9808917197452, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789665, Length=382, Percent_Identity=28.2722513089005, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082036, Length=348, Percent_Identity=23.8505747126437, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081796, Length=385, Percent_Identity=27.7922077922078, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081951, Length=168, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789637, Length=158, Percent_Identity=31.6455696202532, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40049; Mature: 40049

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLNKKWMRLMVLVGCAALTASCKQAPVAQMEAEYAVLKVSPSDKVLSTTYSATIRGRQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEEEEEEECCCC
DIDIYPQVSGFLTRLCVEEGQAVRKGQVLFIIDQVPYKAALETAVANVESAKAGLATAEL
CCEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEE
TYNSKKELFAKKVVSEFDLKTAENSYLTAKAGLAQAKAQEVSARNNLSYTEVKSPSDGVI
ECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCE
GTLPYRVGALVSASLPQPLTTVSDNSDMYVYFSMTENQLLALTRQYGSKAEALKNMPEVE
EECCHHHHHHHCCCCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHCCCCEE
LQLNDKSMYGEKGRIETISGVVDRNTGTASLRAVFPNKNGLLYSGTSGNVILPVTMKGSL
EEECCCHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEEEECCCCCEEEEEEECCCE
VIPQATTFEIQDITYVYKVVDGKAQSAPVSVTRVNGGQEYIVNDGLKEGDIIVAEGVGLL
ECCCCCEEEEEEEEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCEEEECCCCCC
REGTPIKVKQN
CCCCEEEEECC
>Mature Secondary Structure
MMTLNKKWMRLMVLVGCAALTASCKQAPVAQMEAEYAVLKVSPSDKVLSTTYSATIRGRQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEEEEEEECCCC
DIDIYPQVSGFLTRLCVEEGQAVRKGQVLFIIDQVPYKAALETAVANVESAKAGLATAEL
CCEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEE
TYNSKKELFAKKVVSEFDLKTAENSYLTAKAGLAQAKAQEVSARNNLSYTEVKSPSDGVI
ECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCE
GTLPYRVGALVSASLPQPLTTVSDNSDMYVYFSMTENQLLALTRQYGSKAEALKNMPEVE
EECCHHHHHHHCCCCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHCCCCEE
LQLNDKSMYGEKGRIETISGVVDRNTGTASLRAVFPNKNGLLYSGTSGNVILPVTMKGSL
EEECCCHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEEEECCCCCEEEEEEECCCE
VIPQATTFEIQDITYVYKVVDGKAQSAPVSVTRVNGGQEYIVNDGLKEGDIIVAEGVGLL
ECCCCCEEEEEEEEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCEEEECCCCCC
REGTPIKVKQN
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]