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The map label for this gene is ruvA

Identifier: 150004217

GI number: 150004217

Start: 2142080

End: 2142673

Strand: Reverse

Name: ruvA

Synonym: BVU_1658

Alternate gene names: 150004217

Gene position: 2142673-2142080 (Counterclockwise)

Preceding gene: 150004220

Following gene: 150004215

Centisome position: 41.5

GC content: 48.15

Gene sequence:

>594_bases
ATGATAGAATATATTAAAGGAGACATTGCGGAGATTACTCCGGCAACGGTGGTTTTAGATTGTAACGGGATGGGGTATGG
CATCAATATCTCATTGAATACGTATTCGGCCATTCAGAATCAGAGCAATACGAAATTATATATTTATGAAGCGATCCGGG
AAGATGCCTACGTACTCTACGGATTCTCGACCAAACAGGAACGGGAGTTGTTTTTGTTACTGATTTCCGTATCGGGCATC
GGCGGCAACACGGCACGTATGATTCTGTCGGCCTTATCCCCTTCCGAGCTGTGCGGAGTAATCAGTTCCGGTAATGACAA
GTTATTGAAAACGGTAAAAGGCATCGGCCTGAAAACTGCCCAACGCATCATAGTGGACTTAAAGGACAAGATCGCCACTT
CGGGCGTGGAGACGGTGAACAGTGAAATGTTTGCCAATCCGGGCAATACGGAAATTCATGACGAGGCTGTCGCCGCACTC
ACCATGCTGGGATTCGCACAAGCCGCATCACAAAAGGTAGTGGCTGCCATACTGAAAGAGGAGCCTGCCGCCCCGGTAGA
GAAAGTGATCAAGCTGGCCTTGAAGCGATTGTAA

Upstream 100 bases:

>100_bases
CTACGACCTAATAATTTAAAAATATTCTTGCCGAACTTATAATTTAAAAGATAGATGTCCTATTTTTGCAAAGAACTGTA
AATTTTCAAATGAAACAACC

Downstream 100 bases:

>100_bases
GGGCTGTACATGAAACAAGGAACACGGACAATGCGGACTTTGGGGAGATTCTCTTCAGGATCCGCATTGTTTGTGTTCCC
ATCCACATTACTTCTCTTCC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MIEYIKGDIAEITPATVVLDCNGMGYGINISLNTYSAIQNQSNTKLYIYEAIREDAYVLYGFSTKQERELFLLLISVSGI
GGNTARMILSALSPSELCGVISSGNDKLLKTVKGIGLKTAQRIIVDLKDKIATSGVETVNSEMFANPGNTEIHDEAVAAL
TMLGFAQAASQKVVAAILKEEPAAPVEKVIKLALKRL

Sequences:

>Translated_197_residues
MIEYIKGDIAEITPATVVLDCNGMGYGINISLNTYSAIQNQSNTKLYIYEAIREDAYVLYGFSTKQERELFLLLISVSGI
GGNTARMILSALSPSELCGVISSGNDKLLKTVKGIGLKTAQRIIVDLKDKIATSGVETVNSEMFANPGNTEIHDEAVAAL
TMLGFAQAASQKVVAAILKEEPAAPVEKVIKLALKRL
>Mature_197_residues
MIEYIKGDIAEITPATVVLDCNGMGYGINISLNTYSAIQNQSNTKLYIYEAIREDAYVLYGFSTKQERELFLLLISVSGI
GGNTARMILSALSPSELCGVISSGNDKLLKTVKGIGLKTAQRIIVDLKDKIATSGVETVNSEMFANPGNTEIHDEAVAAL
TMLGFAQAASQKVVAAILKEEPAAPVEKVIKLALKRL

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=207, Percent_Identity=30.9178743961353, Blast_Score=103, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_BACV8 (A6L0X5)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298961.1
- ProteinModelPortal:   A6L0X5
- SMR:   A6L0X5
- STRING:   A6L0X5
- GeneID:   5302624
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1658
- NMPDR:   fig|435590.6.peg.1564
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21141; Mature: 21141

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEYIKGDIAEITPATVVLDCNGMGYGINISLNTYSAIQNQSNTKLYIYEAIREDAYVLY
CCCCCCCCCHHCCCEEEEEECCCCCEEEEEEECHHHHHCCCCCCEEEEEEECCCCEEEEE
GFSTKQERELFLLLISVSGIGGNTARMILSALSPSELCGVISSGNDKLLKTVKGIGLKTA
ECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
QRIIVDLKDKIATSGVETVNSEMFANPGNTEIHDEAVAALTMLGFAQAASQKVVAAILKE
HHHHHHHHHHHHHCCHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPAAPVEKVIKLALKRL
CCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIEYIKGDIAEITPATVVLDCNGMGYGINISLNTYSAIQNQSNTKLYIYEAIREDAYVLY
CCCCCCCCCHHCCCEEEEEECCCCCEEEEEEECHHHHHCCCCCCEEEEEEECCCCEEEEE
GFSTKQERELFLLLISVSGIGGNTARMILSALSPSELCGVISSGNDKLLKTVKGIGLKTA
ECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
QRIIVDLKDKIATSGVETVNSEMFANPGNTEIHDEAVAALTMLGFAQAASQKVVAAILKE
HHHHHHHHHHHHHCCHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPAAPVEKVIKLALKRL
CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA