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The map label for this gene is punA [H]

Identifier: 150004166

GI number: 150004166

Start: 2086007

End: 2086816

Strand: Reverse

Name: punA [H]

Synonym: BVU_1602

Alternate gene names: 150004166

Gene position: 2086816-2086007 (Counterclockwise)

Preceding gene: 150004167

Following gene: 150004165

Centisome position: 40.42

GC content: 42.22

Gene sequence:

>810_bases
ATGTTAGAAAAAATTCAAGAAACAGCAACTTTTCTTAGAGAAAAGATGCATACCAGTCCCGAAACTGCTATTATATTAGG
TACCGGCTTAGGAAGCCTGGTACACGAAATTACCGATAAATACGAGATCAGTTACAAAGATATCCCTAATTTCCCTGTTT
CAACCGTAGAGGGACATAGCGGTAAATTGATTTTCGGCAAATTAGGTAATAAAGACATCATGGCTATGCAAGGACGTTTC
CACTTCTATGAAGGATACTCCATGAAAGAAGTTACTTTCCCCGTACGGGTGATGCGTGAATTAGGCATCAAAACATTGTT
CGTATCCAATGCAGCTGGAGGTACGAATGCAGACTTTGAAATTGGTGATCTGATGATTATCAGAGATCATATCAACTTTT
TTCCCGAACATCCGTTGCACGGCAAGAACATTGAATACGGCCCCCGTTTCCCGGATATGAGTGAAGCCTACTCCAAAGAA
CTGATAGACAAAGCACTGGAGATCGCTAAAGAAAAAGGTATCAAAGTACAACAAGGAGTATATATCGGTACACAGGGACC
TACCTTCGAAACTCCGGCAGAATACAAGATGTTCCACATTCTTGGAGCTGACGCCGTAGGTATGTCTACCGTTCCTGAAG
TTATTGTAGCAAACCATTGTGGCATCAAAGTGTTTGGAGTATCGGTTATCACCGACCTAGGCGTGGAGGGAAAAATTGTG
GAAGTTTCACACGAAGAGGTTCAGAAAGCGGCAGATGAAGCGCAACCGCGCATGACTACCATTATGCGTGAAATGATTAA
TCGAATTTAA

Upstream 100 bases:

>100_bases
CTGATGGACGAAATGCTGAAACCTTATATATATATGCTGCCCATTGAGGTAGCCTTTTTACAAGACCAACAAGAACTGTT
TAACTCAAATATAACCGACT

Downstream 100 bases:

>100_bases
GAACATGCAGGAAATAAACAGAACAGAAATAGCGACCTTAGGTGAGTTCGGCCTCATTAAACATCTAACGAAGGATATCG
AGTTAAAAAATCCGGAAACC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MLEKIQETATFLREKMHTSPETAIILGTGLGSLVHEITDKYEISYKDIPNFPVSTVEGHSGKLIFGKLGNKDIMAMQGRF
HFYEGYSMKEVTFPVRVMRELGIKTLFVSNAAGGTNADFEIGDLMIIRDHINFFPEHPLHGKNIEYGPRFPDMSEAYSKE
LIDKALEIAKEKGIKVQQGVYIGTQGPTFETPAEYKMFHILGADAVGMSTVPEVIVANHCGIKVFGVSVITDLGVEGKIV
EVSHEEVQKAADEAQPRMTTIMREMINRI

Sequences:

>Translated_269_residues
MLEKIQETATFLREKMHTSPETAIILGTGLGSLVHEITDKYEISYKDIPNFPVSTVEGHSGKLIFGKLGNKDIMAMQGRF
HFYEGYSMKEVTFPVRVMRELGIKTLFVSNAAGGTNADFEIGDLMIIRDHINFFPEHPLHGKNIEYGPRFPDMSEAYSKE
LIDKALEIAKEKGIKVQQGVYIGTQGPTFETPAEYKMFHILGADAVGMSTVPEVIVANHCGIKVFGVSVITDLGVEGKIV
EVSHEEVQKAADEAQPRMTTIMREMINRI
>Mature_269_residues
MLEKIQETATFLREKMHTSPETAIILGTGLGSLVHEITDKYEISYKDIPNFPVSTVEGHSGKLIFGKLGNKDIMAMQGRF
HFYEGYSMKEVTFPVRVMRELGIKTLFVSNAAGGTNADFEIGDLMIIRDHINFFPEHPLHGKNIEYGPRFPDMSEAYSKE
LIDKALEIAKEKGIKVQQGVYIGTQGPTFETPAEYKMFHILGADAVGMSTVPEVIVANHCGIKVFGVSVITDLGVEGKIV
EVSHEEVQKAADEAQPRMTTIMREMINRI

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=264, Percent_Identity=48.8636363636364, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI47132622, Length=260, Percent_Identity=28.0769230769231, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1788746, Length=252, Percent_Identity=40.4761904761905, Blast_Score=201, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17541190, Length=278, Percent_Identity=42.4460431654676, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323238, Length=229, Percent_Identity=47.5982532751092, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45552887, Length=277, Percent_Identity=46.5703971119134, Blast_Score=245, Evalue=2e-65,
Organism=Drosophila melanogaster, GI45552885, Length=277, Percent_Identity=46.5703971119134, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24656090, Length=277, Percent_Identity=46.5703971119134, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24656093, Length=277, Percent_Identity=46.5703971119134, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24762376, Length=236, Percent_Identity=35.1694915254237, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29953; Mature: 29953

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKIQETATFLREKMHTSPETAIILGTGLGSLVHEITDKYEISYKDIPNFPVSTVEGHS
CCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC
GKLIFGKLGNKDIMAMQGRFHFYEGYSMKEVTFPVRVMRELGIKTLFVSNAAGGTNADFE
CCEEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
IGDLMIIRDHINFFPEHPLHGKNIEYGPRFPDMSEAYSKELIDKALEIAKEKGIKVQQGV
ECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCE
YIGTQGPTFETPAEYKMFHILGADAVGMSTVPEVIVANHCGIKVFGVSVITDLGVEGKIV
EECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHCCCCCEEE
EVSHEEVQKAADEAQPRMTTIMREMINRI
ECCHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEKIQETATFLREKMHTSPETAIILGTGLGSLVHEITDKYEISYKDIPNFPVSTVEGHS
CCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC
GKLIFGKLGNKDIMAMQGRFHFYEGYSMKEVTFPVRVMRELGIKTLFVSNAAGGTNADFE
CCEEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
IGDLMIIRDHINFFPEHPLHGKNIEYGPRFPDMSEAYSKELIDKALEIAKEKGIKVQQGV
ECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCE
YIGTQGPTFETPAEYKMFHILGADAVGMSTVPEVIVANHCGIKVFGVSVITDLGVEGKIV
EECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHCCCCCEEE
EVSHEEVQKAADEAQPRMTTIMREMINRI
ECCHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]