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The map label for this gene is wecG [H]

Identifier: 150004112

GI number: 150004112

Start: 2035471

End: 2036169

Strand: Direct

Name: wecG [H]

Synonym: BVU_1547

Alternate gene names: 150004112

Gene position: 2035471-2036169 (Clockwise)

Preceding gene: 150004111

Following gene: 150004113

Centisome position: 39.42

GC content: 42.78

Gene sequence:

>699_bases
ATGGATAAAGTTTCATTAAACGGGGTCGAAATATTCCCTTTCGACTCCGAACAGCAACTATTGCATTATGTGGACACGCA
TAAGGGCATATTGGTAGCGATCAATGCAGAAAAGATATTGCATGCCACGGAACAGACACGTGCCATTATCAATCGAAATA
TTGGGTATTGTGATGGAGCCGGTGCACAAATGGCTTTAAAGCAGAAAGGTTATAAAGATGCCTGTAAGATTCCCGGATGT
GAACTATGGCTAAAGATTATTACTCGCTTTTACAAGGAAAAGACATTCTATCTGGTTGGTGGAAAACCGCAAATCGTAAA
TGAGACCGTAGAGAAACTGTGTTCTGAATATCAGGATATCCGAATAGTGGGTTATCGTAACGGCTATATCAAGACGGATG
AAGAGAAACGACGATTGATAGACGATATTGTGGAGAAGAAACCGGATGTGGTATTCGTGGCTATGGGGTCGCCAAAGCAG
GAATTGCTGATGGAGGAGATACAGCAACGGCATCGCGCTATCTTCCAAGGATTGGGTGGCAGTTTCGATGTCTATACAGG
TCATGTGCAGCGCGCACCGAAATGGTGGGTGGAGCATAATCTGGAATTTGCTTATCGCTTGATAAAAGAACCCAAAAGAA
TTAAACGTCAGATCCACTTGGTGAAGTATGCTTGGTGGCTGATGAAGAAAAAATTATAA

Upstream 100 bases:

>100_bases
GCCGGGGATAAGTTCAAAAATATCATATATGCGTTGGAGCAATGCGTTTATATTTAATGCTATGAGCCATTATTTACTTG
CCGAATAATATTTTAATCTT

Downstream 100 bases:

>100_bases
ACAGAGGAAATATGAAGAAAGTGATGTTGGTCTTCGGCACCCGTCCGGAGGCAATCAAGATGGCTCCGCTGGTGAAAGAA
TTCCAGAAACAGCCAAAACG

Product: glycosyl transferase family protein

Products: C55-PP-GlcNAc-ManNAcA; UDP [C]

Alternate protein names: UDP-ManNAcA transferase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MDKVSLNGVEIFPFDSEQQLLHYVDTHKGILVAINAEKILHATEQTRAIINRNIGYCDGAGAQMALKQKGYKDACKIPGC
ELWLKIITRFYKEKTFYLVGGKPQIVNETVEKLCSEYQDIRIVGYRNGYIKTDEEKRRLIDDIVEKKPDVVFVAMGSPKQ
ELLMEEIQQRHRAIFQGLGGSFDVYTGHVQRAPKWWVEHNLEFAYRLIKEPKRIKRQIHLVKYAWWLMKKKL

Sequences:

>Translated_232_residues
MDKVSLNGVEIFPFDSEQQLLHYVDTHKGILVAINAEKILHATEQTRAIINRNIGYCDGAGAQMALKQKGYKDACKIPGC
ELWLKIITRFYKEKTFYLVGGKPQIVNETVEKLCSEYQDIRIVGYRNGYIKTDEEKRRLIDDIVEKKPDVVFVAMGSPKQ
ELLMEEIQQRHRAIFQGLGGSFDVYTGHVQRAPKWWVEHNLEFAYRLIKEPKRIKRQIHLVKYAWWLMKKKL
>Mature_232_residues
MDKVSLNGVEIFPFDSEQQLLHYVDTHKGILVAINAEKILHATEQTRAIINRNIGYCDGAGAQMALKQKGYKDACKIPGC
ELWLKIITRFYKEKTFYLVGGKPQIVNETVEKLCSEYQDIRIVGYRNGYIKTDEEKRRLIDDIVEKKPDVVFVAMGSPKQ
ELLMEEIQQRHRAIFQGLGGSFDVYTGHVQRAPKWWVEHNLEFAYRLIKEPKRIKRQIHLVKYAWWLMKKKL

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis [H]

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family [H]

Homologues:

Organism=Escherichia coli, GI2367289, Length=222, Percent_Identity=38.2882882882883, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023085
- InterPro:   IPR004629 [H]

Pfam domain/function: PF03808 Glyco_tran_WecB [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 27113; Mature: 27113

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVSLNGVEIFPFDSEQQLLHYVDTHKGILVAINAEKILHATEQTRAIINRNIGYCDGA
CCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
GAQMALKQKGYKDACKIPGCELWLKIITRFYKEKTFYLVGGKPQIVNETVEKLCSEYQDI
CCHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCE
RIVGYRNGYIKTDEEKRRLIDDIVEKKPDVVFVAMGSPKQELLMEEIQQRHRAIFQGLGG
EEEEECCCCEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
SFDVYTGHVQRAPKWWVEHNLEFAYRLIKEPKRIKRQIHLVKYAWWLMKKKL
CCEEEECCHHCCCHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKVSLNGVEIFPFDSEQQLLHYVDTHKGILVAINAEKILHATEQTRAIINRNIGYCDGA
CCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
GAQMALKQKGYKDACKIPGCELWLKIITRFYKEKTFYLVGGKPQIVNETVEKLCSEYQDI
CCHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCE
RIVGYRNGYIKTDEEKRRLIDDIVEKKPDVVFVAMGSPKQELLMEEIQQRHRAIFQGLGG
EEEEECCCCEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
SFDVYTGHVQRAPKWWVEHNLEFAYRLIKEPKRIKRQIHLVKYAWWLMKKKL
CCEEEECCHHCCCHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosaminuronic-acid; undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate [C]

Specific reaction: UDP-N-acetyl-D-mannosaminuronic-acid + undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate = C55-PP-GlcNAc-ManNAcA + UDP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]