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The map label for this gene is phoB [H]

Identifier: 150004058

GI number: 150004058

Start: 1977712

End: 1979130

Strand: Reverse

Name: phoB [H]

Synonym: BVU_1493

Alternate gene names: 150004058

Gene position: 1979130-1977712 (Counterclockwise)

Preceding gene: 150004061

Following gene: 150004054

Centisome position: 38.33

GC content: 41.86

Gene sequence:

>1419_bases
ATGAAAAAACTATCGTTTATTATTGTATTTGTATTCCTGTCCATTCTTTTTGTACAGGCACAGCAAGCAAAGTATGTATT
TTATTTCATCGGAGATGGAATGGGTGTTAACCAAGTTTTAGGAACAGAGATGTATCGTGGAGAGTTGGAAGGCAAAATTG
GTGTGACTCCGTTATTATTCACCCAATTTCCTTATGCAACCATAGCCACTACTTTCTCGGCAACCAACGGAGTGACAGAT
TCGGCCGCTGCAGGAACAGCACTTGCTACGGGAAACAAAACAAAAAATGGCGCATTAGGTGTGAAAAAGGATCTGGAAAC
CAAAGTTAACAGTGTTGCTTCTTGGGCAAAAAATAAAGGTTGCCGTGTAGGTATCAGTACCAGTGTCAGCGTAGACCATG
CCACACCGGCTGCTTTTTACGCACACCAAGGACAAAGAAGCAGCTATTACAACGTGGGTCTGGATTTGATTGATGCCAAC
TTCGATTTCTATGCAGGATCAGACTTTTTAGATCCCACCAACAAAAAAGCCGCCGGTTCAAATTCAGAAAGTCTGTACAC
ACTGGTGGACAAAGCCGGATATACCATAGCCCGTGGCTACAAGGACTATCAGAAAAAAGCAAAAAAATCGGACAAACTGA
TTCTCTTACAACCTGCGACAGCAACAGACAATAGTGCTATCCCCTATGCTATCGATCGAAAAAAAGGCGATATGACTTTA
ACTGAAATCACCCGTGCCGGAATCAATTTCCTGTCTAAAGATCTATCAAAAGGGTTCTTCCTGATGGTTGAAGGAGGTAA
AATAGACTGGGCTTGCCACAGCAACGACGCCGCTACCGTATTCCACGAAGTGATGGATATGGACAATGCCATAAAAGTTG
CTTACGAATTCTATGAACAACATCCCGATGAAACCCTGATTGTAGTAACAGCCGACCACGAAACCGGAGGAATCGTTTTA
GGAAAAGGTCCGTATACTTTGAACCTGCAAGCTTTAAAATCACAAAAAGTAAGTGAAAGCGGATATACTAAAATTGTAAA
CGAATTAAGAAAAAAATACAAGAATCAAGTACCTTGGGAAGTTATCAAGCAATCATTGAAGGATAATTTCGGATTTTGGG
ATAGTATTCAATTGAATGAAAAGCAAGAAGCATCCCTGAAGAAAGTATATGATGAATCATTTAGCGGTAAAGAGATAGAT
TTGACCAAAAGTGAATATCAACAAGACGAACCTTTGGCAGCTGAGGCCAAAAGAATTCTGGATGACATCGCTCTGGTAGG
TTGGACCAGTGGTGGGCACTCTGGAGGTTATGTACCCGTATTCGCTATCGGAGCCGGCGCCCAACTATTCCAAGGTCGCA
TTGACAATACCGAAATTCCGGTAAAAATAGCAGAAGCTGCTGGTTATCCCAACAATTAA

Upstream 100 bases:

>100_bases
TTGGCCATTAAATCTCAATTATTCAAGAAAGAAGTACCGATAAAAAGTCTTTTTTCCCTATATTTGCTAGGCTGAAAAAA
ATGTCATTAACTCAATTAAT

Downstream 100 bases:

>100_bases
TCAGGGATATTCAGTAAATGTCTCTAAAAAATAAGATGGCATATGAAGATGATAGGGCATGACCATTTCATATGCCATTC
TTATTGTCATTTTTCCGTGC

Product: alkaline phosphatase III precursor

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKKLSFIIVFVFLSILFVQAQQAKYVFYFIGDGMGVNQVLGTEMYRGELEGKIGVTPLLFTQFPYATIATTFSATNGVTD
SAAAGTALATGNKTKNGALGVKKDLETKVNSVASWAKNKGCRVGISTSVSVDHATPAAFYAHQGQRSSYYNVGLDLIDAN
FDFYAGSDFLDPTNKKAAGSNSESLYTLVDKAGYTIARGYKDYQKKAKKSDKLILLQPATATDNSAIPYAIDRKKGDMTL
TEITRAGINFLSKDLSKGFFLMVEGGKIDWACHSNDAATVFHEVMDMDNAIKVAYEFYEQHPDETLIVVTADHETGGIVL
GKGPYTLNLQALKSQKVSESGYTKIVNELRKKYKNQVPWEVIKQSLKDNFGFWDSIQLNEKQEASLKKVYDESFSGKEID
LTKSEYQQDEPLAAEAKRILDDIALVGWTSGGHSGGYVPVFAIGAGAQLFQGRIDNTEIPVKIAEAAGYPNN

Sequences:

>Translated_472_residues
MKKLSFIIVFVFLSILFVQAQQAKYVFYFIGDGMGVNQVLGTEMYRGELEGKIGVTPLLFTQFPYATIATTFSATNGVTD
SAAAGTALATGNKTKNGALGVKKDLETKVNSVASWAKNKGCRVGISTSVSVDHATPAAFYAHQGQRSSYYNVGLDLIDAN
FDFYAGSDFLDPTNKKAAGSNSESLYTLVDKAGYTIARGYKDYQKKAKKSDKLILLQPATATDNSAIPYAIDRKKGDMTL
TEITRAGINFLSKDLSKGFFLMVEGGKIDWACHSNDAATVFHEVMDMDNAIKVAYEFYEQHPDETLIVVTADHETGGIVL
GKGPYTLNLQALKSQKVSESGYTKIVNELRKKYKNQVPWEVIKQSLKDNFGFWDSIQLNEKQEASLKKVYDESFSGKEID
LTKSEYQQDEPLAAEAKRILDDIALVGWTSGGHSGGYVPVFAIGAGAQLFQGRIDNTEIPVKIAEAAGYPNN
>Mature_472_residues
MKKLSFIIVFVFLSILFVQAQQAKYVFYFIGDGMGVNQVLGTEMYRGELEGKIGVTPLLFTQFPYATIATTFSATNGVTD
SAAAGTALATGNKTKNGALGVKKDLETKVNSVASWAKNKGCRVGISTSVSVDHATPAAFYAHQGQRSSYYNVGLDLIDAN
FDFYAGSDFLDPTNKKAAGSNSESLYTLVDKAGYTIARGYKDYQKKAKKSDKLILLQPATATDNSAIPYAIDRKKGDMTL
TEITRAGINFLSKDLSKGFFLMVEGGKIDWACHSNDAATVFHEVMDMDNAIKVAYEFYEQHPDETLIVVTADHETGGIVL
GKGPYTLNLQALKSQKVSESGYTKIVNELRKKYKNQVPWEVIKQSLKDNFGFWDSIQLNEKQEASLKKVYDESFSGKEID
LTKSEYQQDEPLAAEAKRILDDIALVGWTSGGHSGGYVPVFAIGAGAQLFQGRIDNTEIPVKIAEAAGYPNN

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=332, Percent_Identity=30.7228915662651, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI294660770, Length=326, Percent_Identity=30.3680981595092, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI294660772, Length=283, Percent_Identity=29.6819787985866, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI157266292, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI94721246, Length=137, Percent_Identity=34.3065693430657, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157266296, Length=137, Percent_Identity=35.036496350365, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320689, Length=503, Percent_Identity=26.8389662027833, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24651554, Length=482, Percent_Identity=28.2157676348548, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19921912, Length=494, Percent_Identity=27.1255060728745, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24651556, Length=469, Percent_Identity=27.7185501066098, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355981, Length=505, Percent_Identity=27.5247524752475, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358067, Length=486, Percent_Identity=26.9547325102881, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI18859923, Length=464, Percent_Identity=23.7068965517241, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355149, Length=509, Percent_Identity=23.5756385068762, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI85725200, Length=498, Percent_Identity=26.1044176706827, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI85815833, Length=498, Percent_Identity=26.1044176706827, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21355151, Length=496, Percent_Identity=27.2177419354839, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24657842, Length=340, Percent_Identity=26.7647058823529, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24657835, Length=337, Percent_Identity=27.5964391691395, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24657827, Length=489, Percent_Identity=26.1758691206544, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585213, Length=346, Percent_Identity=27.4566473988439, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51613; Mature: 51613

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSFIIVFVFLSILFVQAQQAKYVFYFIGDGMGVNQVLGTEMYRGELEGKIGVTPLLF
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
TQFPYATIATTFSATNGVTDSAAAGTALATGNKTKNGALGVKKDLETKVNSVASWAKNKG
HCCCCHHHHEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
CRVGISTSVSVDHATPAAFYAHQGQRSSYYNVGLDLIDANFDFYAGSDFLDPTNKKAAGS
CEEEEECEEEECCCCCHHHHHCCCCCCCEEECCEEEEECCCCEECCCCCCCCCCCCCCCC
NSESLYTLVDKAGYTIARGYKDYQKKAKKSDKLILLQPATATDNSAIPYAIDRKKGDMTL
CCCCEEEEHHHCCCEEHHCHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCHH
TEITRAGINFLSKDLSKGFFLMVEGGKIDWACHSNDAATVFHEVMDMDNAIKVAYEFYEQ
HHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHH
HPDETLIVVTADHETGGIVLGKGPYTLNLQALKSQKVSESGYTKIVNELRKKYKNQVPWE
CCCCEEEEEEECCCCCCEEECCCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHH
VIKQSLKDNFGFWDSIQLNEKQEASLKKVYDESFSGKEIDLTKSEYQQDEPLAAEAKRIL
HHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCHHHHHHHHH
DDIALVGWTSGGHSGGYVPVFAIGAGAQLFQGRIDNTEIPVKIAEAAGYPNN
HHHHEEEECCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKKLSFIIVFVFLSILFVQAQQAKYVFYFIGDGMGVNQVLGTEMYRGELEGKIGVTPLLF
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
TQFPYATIATTFSATNGVTDSAAAGTALATGNKTKNGALGVKKDLETKVNSVASWAKNKG
HCCCCHHHHEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
CRVGISTSVSVDHATPAAFYAHQGQRSSYYNVGLDLIDANFDFYAGSDFLDPTNKKAAGS
CEEEEECEEEECCCCCHHHHHCCCCCCCEEECCEEEEECCCCEECCCCCCCCCCCCCCCC
NSESLYTLVDKAGYTIARGYKDYQKKAKKSDKLILLQPATATDNSAIPYAIDRKKGDMTL
CCCCEEEEHHHCCCEEHHCHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCHH
TEITRAGINFLSKDLSKGFFLMVEGGKIDWACHSNDAATVFHEVMDMDNAIKVAYEFYEQ
HHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHH
HPDETLIVVTADHETGGIVLGKGPYTLNLQALKSQKVSESGYTKIVNELRKKYKNQVPWE
CCCCEEEEEEECCCCCCEEECCCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHH
VIKQSLKDNFGFWDSIQLNEKQEASLKKVYDESFSGKEIDLTKSEYQQDEPLAAEAKRIL
HHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCHHHHHHHHH
DDIALVGWTSGGHSGGYVPVFAIGAGAQLFQGRIDNTEIPVKIAEAAGYPNN
HHHHEEEECCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]