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The map label for this gene is dapE [H]

Identifier: 150004048

GI number: 150004048

Start: 1964931

End: 1965995

Strand: Reverse

Name: dapE [H]

Synonym: BVU_1483

Alternate gene names: 150004048

Gene position: 1965995-1964931 (Counterclockwise)

Preceding gene: 150004054

Following gene: 150004039

Centisome position: 38.08

GC content: 46.48

Gene sequence:

>1065_bases
ATGATTGATATAGAATATTACACTTCCGAAGCAACCACATTGCTGAATTCGTTAATCGGTATCCCCTCATTGAGCCGGGA
AGAAGAAGCTGCCGCCGACTTTCTGCAGAACTATATTGAAGAAACAGGTATCATGACCGGTCGTTCGGGCAACAACATTT
GGTGTATCAGTCCGATGTTCGACCTCAACAAACCGACTATTTTACTAAATTCCCACATCGATACCGTAAAACCGGTAAAC
GGATGGCGGAAACATCCTTTCACTCCGAAAGCCGAAAACGGAAAGATTTACGGTTTGGGAAGTAATGACGCCGGCGCCAG
TGTAGTATCCTTATTTCAGGTGTACCGCCATCTCTCTACAACCGAGCAGGCATACAATCTGATCTTTCTTGCCTCATGTG
AAGAAGAAGTTTCCGGAAAAAACGGAATAGAAAGCGTATTGCCCCAACTACCTCCCATCACTTTGGGCATAGTGGGTGAA
CCGACAGAAATGCAGCCTGCCATTGCCGAAAAGGGATTGATGGTGCTGGATGTTACCGCATACGGAAAAGCCGGACATGC
CGCACGCAATGAAGGAGTGAATGCTATTTACAAAGTACTGGAAGACATTCAGTGGTTCCGTGATTATAAATTCCCGAAGG
AATCCTCCCTGCTAGGCCCCGTCAAAATGAGCGTGACACAAATAAATGCCGGCACGCAGCATAATGTCATCCCCGATACT
TGTACTTTCGTTGTGGATATCCGCAGTAATGAATGTTACTCTAACGAAGAACTATTCAAAGAAATAAGTGCCCATCTAAA
AAGCGAGGCGAAAGCCCGTTCTTTCCGCTTGAACTCATCTCATATCAGTGCCGAGCATCCCTTTGTGAAACGCGCGGTAA
AACTAGGACGTGTACCTTTCGGTTCTCCTACTTTGTCCGATCAGGCGTTGATGAAGTTCCCGTCTGTCAAAATCGGTCCG
GGAAAGTCCTCCCGTTCACACACGGCTGATGAATATATCATGGTTTCGGAAATTGAGGAAGCGATAAGGCTTTATATCGA
GATGCTGGACGGACTGGTGCTGTAA

Upstream 100 bases:

>100_bases
AAGTTTTAGTTGCATATCTAACTTAATTACCTACTTTTTTCATCACCGTTTACCACGCAAAAATAGTATCTTTGCAACAC
ATTAAATTATTAAAATCAAG

Downstream 100 bases:

>100_bases
ACGACTTACAAAGACGCTATTTCCCCTATAGACAGACCGGTAACCTGTGCAATTACATCAAATGCAATACCGGCCTGTTT
CATTTTTTTTGCATTCTTGA

Product: putative peptidase/deacetylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MIDIEYYTSEATTLLNSLIGIPSLSREEEAAADFLQNYIEETGIMTGRSGNNIWCISPMFDLNKPTILLNSHIDTVKPVN
GWRKHPFTPKAENGKIYGLGSNDAGASVVSLFQVYRHLSTTEQAYNLIFLASCEEEVSGKNGIESVLPQLPPITLGIVGE
PTEMQPAIAEKGLMVLDVTAYGKAGHAARNEGVNAIYKVLEDIQWFRDYKFPKESSLLGPVKMSVTQINAGTQHNVIPDT
CTFVVDIRSNECYSNEELFKEISAHLKSEAKARSFRLNSSHISAEHPFVKRAVKLGRVPFGSPTLSDQALMKFPSVKIGP
GKSSRSHTADEYIMVSEIEEAIRLYIEMLDGLVL

Sequences:

>Translated_354_residues
MIDIEYYTSEATTLLNSLIGIPSLSREEEAAADFLQNYIEETGIMTGRSGNNIWCISPMFDLNKPTILLNSHIDTVKPVN
GWRKHPFTPKAENGKIYGLGSNDAGASVVSLFQVYRHLSTTEQAYNLIFLASCEEEVSGKNGIESVLPQLPPITLGIVGE
PTEMQPAIAEKGLMVLDVTAYGKAGHAARNEGVNAIYKVLEDIQWFRDYKFPKESSLLGPVKMSVTQINAGTQHNVIPDT
CTFVVDIRSNECYSNEELFKEISAHLKSEAKARSFRLNSSHISAEHPFVKRAVKLGRVPFGSPTLSDQALMKFPSVKIGP
GKSSRSHTADEYIMVSEIEEAIRLYIEMLDGLVL
>Mature_354_residues
MIDIEYYTSEATTLLNSLIGIPSLSREEEAAADFLQNYIEETGIMTGRSGNNIWCISPMFDLNKPTILLNSHIDTVKPVN
GWRKHPFTPKAENGKIYGLGSNDAGASVVSLFQVYRHLSTTEQAYNLIFLASCEEEVSGKNGIESVLPQLPPITLGIVGE
PTEMQPAIAEKGLMVLDVTAYGKAGHAARNEGVNAIYKVLEDIQWFRDYKFPKESSLLGPVKMSVTQINAGTQHNVIPDT
CTFVVDIRSNECYSNEELFKEISAHLKSEAKARSFRLNSSHISAEHPFVKRAVKLGRVPFGSPTLSDQALMKFPSVKIGP
GKSSRSHTADEYIMVSEIEEAIRLYIEMLDGLVL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=369, Percent_Identity=24.1192411924119, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551016, Length=344, Percent_Identity=25.2906976744186, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 39102; Mature: 39102

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIEYYTSEATTLLNSLIGIPSLSREEEAAADFLQNYIEETGIMTGRSGNNIWCISPMF
CEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEEEEECCCC
DLNKPTILLNSHIDTVKPVNGWRKHPFTPKAENGKIYGLGSNDAGASVVSLFQVYRHLST
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
TEQAYNLIFLASCEEEVSGKNGIESVLPQLPPITLGIVGEPTEMQPAIAEKGLMVLDVTA
HHHHHCEEEEEECHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHCCEEEEEEEE
YGKAGHAARNEGVNAIYKVLEDIQWFRDYKFPKESSLLGPVKMSVTQINAGTQHNVIPDT
CCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
CTFVVDIRSNECYSNEELFKEISAHLKSEAKARSFRLNSSHISAEHPFVKRAVKLGRVPF
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCC
GSPTLSDQALMKFPSVKIGPGKSSRSHTADEYIMVSEIEEAIRLYIEMLDGLVL
CCCCCCCHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDIEYYTSEATTLLNSLIGIPSLSREEEAAADFLQNYIEETGIMTGRSGNNIWCISPMF
CEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEEEEECCCC
DLNKPTILLNSHIDTVKPVNGWRKHPFTPKAENGKIYGLGSNDAGASVVSLFQVYRHLST
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
TEQAYNLIFLASCEEEVSGKNGIESVLPQLPPITLGIVGEPTEMQPAIAEKGLMVLDVTA
HHHHHCEEEEEECHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHCCEEEEEEEE
YGKAGHAARNEGVNAIYKVLEDIQWFRDYKFPKESSLLGPVKMSVTQINAGTQHNVIPDT
CCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
CTFVVDIRSNECYSNEELFKEISAHLKSEAKARSFRLNSSHISAEHPFVKRAVKLGRVPF
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCC
GSPTLSDQALMKFPSVKIGPGKSSRSHTADEYIMVSEIEEAIRLYIEMLDGLVL
CCCCCCCHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]