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The map label for this gene is lepB [C]

Identifier: 150003827

GI number: 150003827

Start: 1689897

End: 1690760

Strand: Direct

Name: lepB [C]

Synonym: BVU_1259

Alternate gene names: 150003827

Gene position: 1689897-1690760 (Clockwise)

Preceding gene: 150003826

Following gene: 150003828

Centisome position: 32.73

GC content: 44.91

Gene sequence:

>864_bases
ATGCTTACTGCTGTCATTCTGGTACTGTTGGTAAAGACATTTGCTTTCACCTCCTGCACCATTCCCTCTACCGGTATGGA
GAATAGCCTTTATCAAGGGGAAAGGGTTTTGGTGAATAAGTGGAGTTATGGGTTTCGTGTGCCTTTTTCTATCTGGCGGT
GGCTGGGAAAAACTGCCGGGAAAGGGGATATTGTGTTGTTCAACAATCCTAATCCACGGTCTCCTCAGACGTCGGTGGGC
AATCGGGAAGTGTTTATAAGCCGTGTGGTAGGAGTACCCGGAGATACGTTGATGTTGAATGATGAACTGTGGGTGACCGA
CGAACAAGTGTTGAGCCCGGATAGTAAATCTTTGTATGTCTATTCTCATACGGAAGAAGAAACGATGCAGGCTGCCATGC
AGCAAGTGAACATTCAGGGAAACAGGTTGGTGGGGTATGCCGAGGGGAAGTATATACGTACCTTCAGTCATTATGAATAT
TACCTGTTAAAACAAAAACTGGCGGGAAAAGTGGATTTGATTCCTCTTTATCATAAGGATATAAGCAAGAGCCATCCCTT
TGTGATCCCGGAAAAAGGGAAACCTGTCAAGGTATATCCTTGGAATGTGACCTTGTTGTGCAATACTATTGTCCGCCATG
AAGGGAGAGTGGCTTCTGTCAGGGGAGATACCCTGTATGTAGGGGAAAAACCTGTAGAAGCCTATACATTTAATAAAAAT
TATTACTGGATGGCATCCAATAATCCGGTGAATCTGTGTGATTCGCGTTTATTCGGCCTGGTGCCCGATGACCATTTGAT
CGGTAAAGCTTGGCGCATTTGGTTTTCATCCAGAAAAGGGCGCATTTTTCAACGAGTGCAATAA

Upstream 100 bases:

>100_bases
GGCTGGCTGGATGGAAAAATACGTTGGAACCGATTGTTTACCTTTGTAGATAACATTAAATAAATTGCGCATACCCTGTA
TTCCTGTATGGATAAAAGCA

Downstream 100 bases:

>100_bases
TGGAGAATACGATATATTTAAGTTCGGCCTATCTGGCCCCGGTGGAATATTATACAAAACTGTTTGCCTGTGAGAAGGCC
TATGTGGAACAGTATGACAA

Product: signal peptidase I

Products: NA

Alternate protein names: 30S Ribosomal /Signal Peptidase I; Signal Peptidase; Peptidase; Exported Signal Peptidase I

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLTAVILVLLVKTFAFTSCTIPSTGMENSLYQGERVLVNKWSYGFRVPFSIWRWLGKTAGKGDIVLFNNPNPRSPQTSVG
NREVFISRVVGVPGDTLMLNDELWVTDEQVLSPDSKSLYVYSHTEEETMQAAMQQVNIQGNRLVGYAEGKYIRTFSHYEY
YLLKQKLAGKVDLIPLYHKDISKSHPFVIPEKGKPVKVYPWNVTLLCNTIVRHEGRVASVRGDTLYVGEKPVEAYTFNKN
YYWMASNNPVNLCDSRLFGLVPDDHLIGKAWRIWFSSRKGRIFQRVQ

Sequences:

>Translated_287_residues
MLTAVILVLLVKTFAFTSCTIPSTGMENSLYQGERVLVNKWSYGFRVPFSIWRWLGKTAGKGDIVLFNNPNPRSPQTSVG
NREVFISRVVGVPGDTLMLNDELWVTDEQVLSPDSKSLYVYSHTEEETMQAAMQQVNIQGNRLVGYAEGKYIRTFSHYEY
YLLKQKLAGKVDLIPLYHKDISKSHPFVIPEKGKPVKVYPWNVTLLCNTIVRHEGRVASVRGDTLYVGEKPVEAYTFNKN
YYWMASNNPVNLCDSRLFGLVPDDHLIGKAWRIWFSSRKGRIFQRVQ
>Mature_287_residues
MLTAVILVLLVKTFAFTSCTIPSTGMENSLYQGERVLVNKWSYGFRVPFSIWRWLGKTAGKGDIVLFNNPNPRSPQTSVG
NREVFISRVVGVPGDTLMLNDELWVTDEQVLSPDSKSLYVYSHTEEETMQAAMQQVNIQGNRLVGYAEGKYIRTFSHYEY
YLLKQKLAGKVDLIPLYHKDISKSHPFVIPEKGKPVKVYPWNVTLLCNTIVRHEGRVASVRGDTLYVGEKPVEAYTFNKN
YYWMASNNPVNLCDSRLFGLVPDDHLIGKAWRIWFSSRKGRIFQRVQ

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.21.89

Molecular weight: Translated: 32867; Mature: 32867

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAVILVLLVKTFAFTSCTIPSTGMENSLYQGERVLVNKWSYGFRVPFSIWRWLGKTAG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEECHHHHHHHHCCCCC
KGDIVLFNNPNPRSPQTSVGNREVFISRVVGVPGDTLMLNDELWVTDEQVLSPDSKSLYV
CCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCEEECCHHHCCCCCCEEEE
YSHTEEETMQAAMQQVNIQGNRLVGYAEGKYIRTFSHYEYYLLKQKLAGKVDLIPLYHKD
EECCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCHHEEEHHHHCCCEEEEEEEHHC
ISKSHPFVIPEKGKPVKVYPWNVTLLCNTIVRHEGRVASVRGDTLYVGEKPVEAYTFNKN
CCCCCCEEECCCCCEEEEECCEEEEEEEHHHHCCCEEEEECCCEEEECCCCCEEEEECCE
YYWMASNNPVNLCDSRLFGLVPDDHLIGKAWRIWFSSRKGRIFQRVQ
EEEEECCCCCCCCCCCEECCCCCCHHHCCEEEEEEECCCCCEEECCC
>Mature Secondary Structure
MLTAVILVLLVKTFAFTSCTIPSTGMENSLYQGERVLVNKWSYGFRVPFSIWRWLGKTAG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEECHHHHHHHHCCCCC
KGDIVLFNNPNPRSPQTSVGNREVFISRVVGVPGDTLMLNDELWVTDEQVLSPDSKSLYV
CCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCEEECCHHHCCCCCCEEEE
YSHTEEETMQAAMQQVNIQGNRLVGYAEGKYIRTFSHYEYYLLKQKLAGKVDLIPLYHKD
EECCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCHHEEEHHHHCCCEEEEEEEHHC
ISKSHPFVIPEKGKPVKVYPWNVTLLCNTIVRHEGRVASVRGDTLYVGEKPVEAYTFNKN
CCCCCCEEECCCCCEEEEECCEEEEEEEHHHHCCCEEEEECCCEEEECCCCCEEEEECCE
YYWMASNNPVNLCDSRLFGLVPDDHLIGKAWRIWFSSRKGRIFQRVQ
EEEEECCCCCCCCCCCEECCCCCCHHHCCEEEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phospholipids; Triton X-100 [C]

Metal ions: NA

Kcat value (1/min): 523.8 [C]

Specific activity: NA

Km value (mM): 0.52 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.33 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.032 {pro-OmpA-nuclease} 0.0165 {pro-OmpA-nuclease} 1 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-CONH2}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Peptide bond hydrolysis [C]

Inhibitor: 1-Ethyl -3-(3-dimethyl aminopropyl) carbodiimide; beta-Lactams; Bromosuccinimide; Carboxyphenanthroline; Cholate; Cu2+; Deoxycholate; Diethyl dicarbonate; Dinitrophenol; Hg2+; Mg2+ most serine peptidases; NEM Cys; Phenyl glyoxal; SDS; Sodiumchloride [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA