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The map label for this gene is ispB [H]

Identifier: 150003776

GI number: 150003776

Start: 1628166

End: 1629140

Strand: Direct

Name: ispB [H]

Synonym: BVU_1208

Alternate gene names: 150003776

Gene position: 1628166-1629140 (Clockwise)

Preceding gene: 150003769

Following gene: 150003788

Centisome position: 31.53

GC content: 43.08

Gene sequence:

>975_bases
ATGGACAAATTACAGCAAATCAAATTGCCTATTGACAAGGAGTTTGAGGAATTTAGAAGGTTGTTTGACTCTTCGCTCCA
AAGTTCCAATTCTTTACTTAGTGAGGTGTTATCCTATATTAAGCAGAGAAATGGCAAGATGATGCGTCCCATATTGGCGT
TGTTGATTGCTAAATTATTCGGTGAAATCAATGATTCTACGCTTCATGCGGCTCTTTCATTGGAGTTGCTCCATACGGCC
AGCCTGGTGCACGATGATGTGGTGGATGAGAGTGACAAGCGCCGTGGGCAGTCTTCGGTAAATGCTATCTATAATAATAA
GGTGTCGGTTCTGGTAGGCGACTATATGCTGGCAACCAGCCTGAAGCATTCTGCCATGACCCGCGAGATTACAATTGTAG
ATTTGGTGGCTTGTTTGGGACAGAACTTATCTGAAGGCGAGATTATTCAATTGGCGAATATTAATGCTTCTGAGTTCTCG
GAAGAAGTGTATTATGATGTCATTCGTAAAAAGACGGCTGCATTGTTTACTGCCAGTGCGGAAGCGGGGGCTATTTCGGT
GCATGCATCCGATGAAATGGTGAAAAATGCTCGTCTGTTTGGCGAAATGATTGGTATTGCTTTCCAGATAAAGGATGATA
TTTTCGACTATTATTCTTCTGATGAGATAGGTAAACCTACCGGGAACGATATGCGTGAAGGGAAGCTGACATTGCCGGCT
TTGTATGTGTTGAATATGTTTGATGATGAGGAGATGCGGAAACTGGCGCTACGCATCCGGGCATTAGACGCTTCGGATGA
AGATATTGCCCGTTTCATCGAATATACTAAGGTGAAGGGAGGGATAGAGTATGCCAGGCAGGCAATGGTGGATTATCGTA
ATAAAGCGTTGGCTTTACTTCCACAATCAGCCGGTCAAGCTGTAAAAGATGCTTTGACTGCTTATATTGATTATGTGATT
GAAAGAGATAAATAG

Upstream 100 bases:

>100_bases
TTTGTCCATTCTTAAAAATCTTTAGTTTTTTCATTGTAAAAGTACAAAAAAATACCGTATTCCGTCTTTTATTACTACTT
TTGTGCACAAATTAACCTGC

Downstream 100 bases:

>100_bases
TACGATTGTTGAAGATATGTACTTCAAAAAGTTCGGGCGTAGTCCTTTATACAATAAGGACTACGCCCGAACTTTTTGAG
GTACGCCTTTCTTTGGTAGA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MDKLQQIKLPIDKEFEEFRRLFDSSLQSSNSLLSEVLSYIKQRNGKMMRPILALLIAKLFGEINDSTLHAALSLELLHTA
SLVHDDVVDESDKRRGQSSVNAIYNNKVSVLVGDYMLATSLKHSAMTREITIVDLVACLGQNLSEGEIIQLANINASEFS
EEVYYDVIRKKTAALFTASAEAGAISVHASDEMVKNARLFGEMIGIAFQIKDDIFDYYSSDEIGKPTGNDMREGKLTLPA
LYVLNMFDDEEMRKLALRIRALDASDEDIARFIEYTKVKGGIEYARQAMVDYRNKALALLPQSAGQAVKDALTAYIDYVI
ERDK

Sequences:

>Translated_324_residues
MDKLQQIKLPIDKEFEEFRRLFDSSLQSSNSLLSEVLSYIKQRNGKMMRPILALLIAKLFGEINDSTLHAALSLELLHTA
SLVHDDVVDESDKRRGQSSVNAIYNNKVSVLVGDYMLATSLKHSAMTREITIVDLVACLGQNLSEGEIIQLANINASEFS
EEVYYDVIRKKTAALFTASAEAGAISVHASDEMVKNARLFGEMIGIAFQIKDDIFDYYSSDEIGKPTGNDMREGKLTLPA
LYVLNMFDDEEMRKLALRIRALDASDEDIARFIEYTKVKGGIEYARQAMVDYRNKALALLPQSAGQAVKDALTAYIDYVI
ERDK
>Mature_324_residues
MDKLQQIKLPIDKEFEEFRRLFDSSLQSSNSLLSEVLSYIKQRNGKMMRPILALLIAKLFGEINDSTLHAALSLELLHTA
SLVHDDVVDESDKRRGQSSVNAIYNNKVSVLVGDYMLATSLKHSAMTREITIVDLVACLGQNLSEGEIIQLANINASEFS
EEVYYDVIRKKTAALFTASAEAGAISVHASDEMVKNARLFGEMIGIAFQIKDDIFDYYSSDEIGKPTGNDMREGKLTLPA
LYVLNMFDDEEMRKLALRIRALDASDEDIARFIEYTKVKGGIEYARQAMVDYRNKALALLPQSAGQAVKDALTAYIDYVI
ERDK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=305, Percent_Identity=31.4754098360656, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1789578, Length=300, Percent_Identity=32.3333333333333, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786623, Length=341, Percent_Identity=28.1524926686217, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=300, Percent_Identity=32.3333333333333, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=293, Percent_Identity=30.3754266211604, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=265, Percent_Identity=29.0566037735849, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24651612, Length=322, Percent_Identity=31.6770186335404, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36284; Mature: 36284

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLQQIKLPIDKEFEEFRRLFDSSLQSSNSLLSEVLSYIKQRNGKMMRPILALLIAKLF
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GEINDSTLHAALSLELLHTASLVHDDVVDESDKRRGQSSVNAIYNNKVSVLVGDYMLATS
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCEEEEEHHHHHHHH
LKHSAMTREITIVDLVACLGQNLSEGEIIQLANINASEFSEEVYYDVIRKKTAALFTASA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECC
EAGAISVHASDEMVKNARLFGEMIGIAFQIKDDIFDYYSSDEIGKPTGNDMREGKLTLPA
CCCEEEEECCHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
LYVLNMFDDEEMRKLALRIRALDASDEDIARFIEYTKVKGGIEYARQAMVDYRNKALALL
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
PQSAGQAVKDALTAYIDYVIERDK
CCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKLQQIKLPIDKEFEEFRRLFDSSLQSSNSLLSEVLSYIKQRNGKMMRPILALLIAKLF
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GEINDSTLHAALSLELLHTASLVHDDVVDESDKRRGQSSVNAIYNNKVSVLVGDYMLATS
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCEEEEEHHHHHHHH
LKHSAMTREITIVDLVACLGQNLSEGEIIQLANINASEFSEEVYYDVIRKKTAALFTASA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECC
EAGAISVHASDEMVKNARLFGEMIGIAFQIKDDIFDYYSSDEIGKPTGNDMREGKLTLPA
CCCEEEEECCHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
LYVLNMFDDEEMRKLALRIRALDASDEDIARFIEYTKVKGGIEYARQAMVDYRNKALALL
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
PQSAGQAVKDALTAYIDYVIERDK
CCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]