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The map label for this gene is truB

Identifier: 150003741

GI number: 150003741

Start: 1586462

End: 1587169

Strand: Direct

Name: truB

Synonym: BVU_1172

Alternate gene names: 150003741

Gene position: 1586462-1587169 (Clockwise)

Preceding gene: 150003740

Following gene: 150003742

Centisome position: 30.73

GC content: 45.9

Gene sequence:

>708_bases
ATGAATTTTAAAGAAGGAGAAGTATTATACTTTGATAAACCGCTGAAATGGACCTCATTTGCAGTGGTGAACAAGATAAG
ATACCATATTTGCCGCAAACTGGGCGTGAAGAAAATAAAAGTGGGACATGCCGGGACATTAGACCCGCTGGCTACCGGGG
TGATGATTATTTGTACAGGCAAGGCAACGAAACGCATCGAAGAGTTCCAGTATCATACCAAAGAATACATTGCCACCTTG
CAGTTGGGGGCTACCACACCTTCATTCGACCTGGAGAAGGAGATAGATGCTACCTATCCTACGGAACATATCACCCGCGA
GTTGGTAGAGGAAGCCTTGCAGCGTTTTATCGGCAGGATAGAACAGATTCCGCCTGTTTTCTCGGCTTGTAAGGTAGATG
GTAAACGCGCTTACGATTTGGCTCGAAAAGGTGAGGATGTGGAGTTGAAAGCCAAGACGTTGATTATAGACGAGATAGAG
TTGCTGGAATGTAATCTGCCTGAGATTAAAATCAGGGTAGTGTGCAGCAAGGGCACTTATATCCGTGCATTGGCCCGTGA
TATTGGCGAAGCTTTGAATAGCGGTGCTCATTTGACAGGGCTGATACGTACCCGGGTGGGGGATGTGAGACTGGAAGACT
GTTTGTCGGTGGAAAGTTTTCCGGAATGGCTTGACCAACAAGAGATTGAAGAAGTTATAAACGAATAA

Upstream 100 bases:

>100_bases
TGGATGATTAATATTGTGAAGAAAGGTAAGTTGATTTATTTTGCTATCTATTGTGCCATAGCCGGTGCCGTTACGATAGC
GTGCACATTGATGTAATTGG

Downstream 100 bases:

>100_bases
GGAGAGATAAGATGAAACTGTCGCAATTTAAATTTAAACTGCCGGAGGATAAAATAGCTTTGCATCCGGCTAAGTATAGA
GATGAGTCGCGCCTGATGGT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MNFKEGEVLYFDKPLKWTSFAVVNKIRYHICRKLGVKKIKVGHAGTLDPLATGVMIICTGKATKRIEEFQYHTKEYIATL
QLGATTPSFDLEKEIDATYPTEHITRELVEEALQRFIGRIEQIPPVFSACKVDGKRAYDLARKGEDVELKAKTLIIDEIE
LLECNLPEIKIRVVCSKGTYIRALARDIGEALNSGAHLTGLIRTRVGDVRLEDCLSVESFPEWLDQQEIEEVINE

Sequences:

>Translated_235_residues
MNFKEGEVLYFDKPLKWTSFAVVNKIRYHICRKLGVKKIKVGHAGTLDPLATGVMIICTGKATKRIEEFQYHTKEYIATL
QLGATTPSFDLEKEIDATYPTEHITRELVEEALQRFIGRIEQIPPVFSACKVDGKRAYDLARKGEDVELKAKTLIIDEIE
LLECNLPEIKIRVVCSKGTYIRALARDIGEALNSGAHLTGLIRTRVGDVRLEDCLSVESFPEWLDQQEIEEVINE
>Mature_235_residues
MNFKEGEVLYFDKPLKWTSFAVVNKIRYHICRKLGVKKIKVGHAGTLDPLATGVMIICTGKATKRIEEFQYHTKEYIATL
QLGATTPSFDLEKEIDATYPTEHITRELVEEALQRFIGRIEQIPPVFSACKVDGKRAYDLARKGEDVELKAKTLIIDEIE
LLECNLPEIKIRVVCSKGTYIRALARDIGEALNSGAHLTGLIRTRVGDVRLEDCLSVESFPEWLDQQEIEEVINE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=217, Percent_Identity=34.5622119815668, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI2367200, Length=227, Percent_Identity=34.8017621145374, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324037, Length=116, Percent_Identity=38.7931034482759, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=216, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BACV8 (A6KZJ9)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298485.1
- ProteinModelPortal:   A6KZJ9
- SMR:   A6KZJ9
- STRING:   A6KZJ9
- GeneID:   5302138
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1172
- NMPDR:   fig|435590.6.peg.1110
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   EYLGTIR
- ProtClustDB:   CLSK2516788
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26652; Mature: 26652

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKEGEVLYFDKPLKWTSFAVVNKIRYHICRKLGVKKIKVGHAGTLDPLATGVMIICTG
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHCCEEEEEEC
KATKRIEEFQYHTKEYIATLQLGATTPSFDLEKEIDATYPTEHITRELVEEALQRFIGRI
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EQIPPVFSACKVDGKRAYDLARKGEDVELKAKTLIIDEIELLECNLPEIKIRVVCSKGTY
HHCCHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEECCHHEEECCCCCEEEEEEECCCHH
IRALARDIGEALNSGAHLTGLIRTRVGDVRLEDCLSVESFPEWLDQQEIEEVINE
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MNFKEGEVLYFDKPLKWTSFAVVNKIRYHICRKLGVKKIKVGHAGTLDPLATGVMIICTG
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHCCEEEEEEC
KATKRIEEFQYHTKEYIATLQLGATTPSFDLEKEIDATYPTEHITRELVEEALQRFIGRI
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EQIPPVFSACKVDGKRAYDLARKGEDVELKAKTLIIDEIELLECNLPEIKIRVVCSKGTY
HHCCHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEECCHHEEECCCCCEEEEEEECCCHH
IRALARDIGEALNSGAHLTGLIRTRVGDVRLEDCLSVESFPEWLDQQEIEEVINE
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA