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The map label for this gene is lacZ [H]

Identifier: 150003720

GI number: 150003720

Start: 1553334

End: 1555772

Strand: Direct

Name: lacZ [H]

Synonym: BVU_1151

Alternate gene names: 150003720

Gene position: 1553334-1555772 (Clockwise)

Preceding gene: 150003719

Following gene: 150003721

Centisome position: 30.08

GC content: 39.36

Gene sequence:

>2439_bases
ATGAAGAAAAAGAAGTTACCATTAGTCTGTTGGGATAGTATCAAATTACTAACCGCACTTTTCCTGTTATGGGGAGTTTG
TTTCGGAGCAGATGCGTATCCGGAAAGTGAATATCGAAAGCAACTTTTTGATTATGATTGGAAATTCAAGTTGGGAGATT
ATCCTGACGCTTCATTGAATACATACGATGACGCTGATTGGAGGATACTTGATTTACCTCATGATTGGAGTATAGAAGGA
ACTCTTGATCCGGAAAATCCAATGGGGAATGATGGAGGTTATTTTCCGGCAGGAACAGGATGGTATCGTAAGTCATTTGA
AATATCTTCTAAGTATAGAGGCAAGAAAGTGGGTATTTACTTTGAAGGAGTTTATATGAATTCAGAAGTCTTTGTTAATG
GAAAATCTATAGGAATTCGTCCTTATGGATATTCATCTTTTTACTATGATCTGACTCCTTATCTGCGTTATGATGATAAG
AATATTATTGCTGTACGGGTAGATAATTCGCAGCAGAAAAATTGTCGCTGGTATACTGGTACAGGCATTTACCGGCATGT
GTGGCTGACAGTGATGAATCCTATTCATATAGAGCATTGGGGGATAGCTATAACTACTCCTGAGGTGAGTGAGGAGAGAG
CTGTTGTACAAATAAAAACTATTTTGAGAAATGAAACTTCTTCTAACAGACAGATTACCTTGACAACAAAGTTGACTAAA
GGAAATGATGAATCTGGAAAAGGAGAAATAAAAGTGGATTTGCCTGCCAATGGAATAAAGGAAATAACACAAAAAATTTC
TGTATTGTATCCTGCTTTGTGGAGTCCTGAGTCCCCTCATTTATATAATGCACACTTTCTAGTGACTGAAGCGGGTGATG
TAATAGATAGTATAACGGAATCTTTTGGTATTCGGACGGTTACTTATTCGGCTGAACAAGGATTATTCTTGAATGGCAAG
AGAATTATTTTAAATGGTGGATGTTTACATCATGACAACGGTTGTCTGGGGGCAGCGGCTTATGATCGTGCTGAAGAACG
GAAAGTAGAATTAATGAAGGCTGCGGGATTTAATGCGGTACGTACTTCGCATAATATTCCTTCAGAAGAGTTCCTCCATG
CCTGTGATCGTCTCGGATTATTGGTTATAGATGAAACCTTTGACGGTTGGCGTGACAGTAAGAATCAATATGACTATTCT
ATTCTTTTTGATCAATGGTGGCAACGGGATATCGAATCCATGGTACTTCGTGACCGCAATCATCCTTCTATTTTTTGTTG
GAGTATTGGTAATGAAGTAATTGAGCGTAAAAAATTGGAAGTGGTCACTACAGCTCGAAAGTTGGCCGACTATGTACATA
AGTTTGATCCCAGTCGTCCGGTAACTTCTGCTTTAGCTGCTTGGGATAATGACTGGGAAATATATGATCCATTGGCTGCC
GTGCATGAAATAGTGGGATATAATTATTTGCTGCATCGAGCGCCGGTTGATCATCAGCGTGTACCTTCCCGTGTTATTAT
GCAAACTGAATCTTACCCACGAGACGTTTTTGCCAATTGGTCATTGGTTAATGATCATGATTATATTATTGGTGATTTTG
TTTGGACAGCTATTGATTATTTAGGTGAATCGGGTATCGGGCGATTTTATTATGCAGGTGAATCGGAAGGTGAACACTAT
CAACGTAACCATTATCCTTGGCATGGGGCCTATTGTGGAGATATTGATTTAACAGGTTGGAGAAAGCCTATATCACATTA
TCGTGACTTGTTGTATAATCCTGATAAGAAACTTTATTTGGCTGTAAAAGAGCCGGATAATTATTATGGTAAGGTGAAGG
AAACCTTATGGTCTGTCTGGCCTACTTGGGAAAGCTGGAATTGGCCGGGACATGAAGGAAAAGAAATAGAGGTAGAGATT
TATTCCCGATATCCCAAAGTACGTCTGTATTTGAATGATAAGCTGATAGGCGAGCAATTTACAGGACAGGAACAGCAATT
CAAAGCAGTCTTTCCTATTTCATACGAGCCAGGAATATTGAAGGCGGTAGGTGTGGAATCCGAGATAGAAATAGAGAAAA
CGATCTTACAGACTGCCGGAAAACCTGTGAAAATTCAATTAACGGCCGATCGTACAAAAATAAAGGCAAATGGTCAGGAT
TTGAGTTTTATCACAGTAGAAATATTAGATAAGGAAGGCATTCTGGAGCCGAATGCTGATAATCAATTAACTTTTGAAGT
GAAAGGGGCAGGTACTATAGTGGCTGTGGGAAATGCTGATTTAAAAGACACGGATAGCTATATCGGTTATCAGCGGAAAG
CATGGAAAGGGCGTGCTATAGTGGTAGTAAAAAGTACACGGAAAGAAGGTAAGATAATGTTGAAAGTGGCTTCTCCCGGA
TTGGTTCCGGCTACTGTATCCATTCGTACAAGTAAATAA

Upstream 100 bases:

>100_bases
CGTTTCATGAAAGGATTTTATACCTTTTGTAAAGTGTTTCGATACCCTGTTGTTTTTAGGATATGGCTTCTTTTGTTTCA
TGATTCTTAAATTATAAAGC

Downstream 100 bases:

>100_bases
TAATAACTTTTGTTTAATTCTAAATATAATAGTATGAAAACATTTCGTTTTGTTTTATTGCTGTTTTTGGTATTGAGTAT
ATCCAATACAGCTTGGGCGC

Product: beta-glycosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 812; Mature: 812

Protein sequence:

>812_residues
MKKKKLPLVCWDSIKLLTALFLLWGVCFGADAYPESEYRKQLFDYDWKFKLGDYPDASLNTYDDADWRILDLPHDWSIEG
TLDPENPMGNDGGYFPAGTGWYRKSFEISSKYRGKKVGIYFEGVYMNSEVFVNGKSIGIRPYGYSSFYYDLTPYLRYDDK
NIIAVRVDNSQQKNCRWYTGTGIYRHVWLTVMNPIHIEHWGIAITTPEVSEERAVVQIKTILRNETSSNRQITLTTKLTK
GNDESGKGEIKVDLPANGIKEITQKISVLYPALWSPESPHLYNAHFLVTEAGDVIDSITESFGIRTVTYSAEQGLFLNGK
RIILNGGCLHHDNGCLGAAAYDRAEERKVELMKAAGFNAVRTSHNIPSEEFLHACDRLGLLVIDETFDGWRDSKNQYDYS
ILFDQWWQRDIESMVLRDRNHPSIFCWSIGNEVIERKKLEVVTTARKLADYVHKFDPSRPVTSALAAWDNDWEIYDPLAA
VHEIVGYNYLLHRAPVDHQRVPSRVIMQTESYPRDVFANWSLVNDHDYIIGDFVWTAIDYLGESGIGRFYYAGESEGEHY
QRNHYPWHGAYCGDIDLTGWRKPISHYRDLLYNPDKKLYLAVKEPDNYYGKVKETLWSVWPTWESWNWPGHEGKEIEVEI
YSRYPKVRLYLNDKLIGEQFTGQEQQFKAVFPISYEPGILKAVGVESEIEIEKTILQTAGKPVKIQLTADRTKIKANGQD
LSFITVEILDKEGILEPNADNQLTFEVKGAGTIVAVGNADLKDTDSYIGYQRKAWKGRAIVVVKSTRKEGKIMLKVASPG
LVPATVSIRTSK

Sequences:

>Translated_812_residues
MKKKKLPLVCWDSIKLLTALFLLWGVCFGADAYPESEYRKQLFDYDWKFKLGDYPDASLNTYDDADWRILDLPHDWSIEG
TLDPENPMGNDGGYFPAGTGWYRKSFEISSKYRGKKVGIYFEGVYMNSEVFVNGKSIGIRPYGYSSFYYDLTPYLRYDDK
NIIAVRVDNSQQKNCRWYTGTGIYRHVWLTVMNPIHIEHWGIAITTPEVSEERAVVQIKTILRNETSSNRQITLTTKLTK
GNDESGKGEIKVDLPANGIKEITQKISVLYPALWSPESPHLYNAHFLVTEAGDVIDSITESFGIRTVTYSAEQGLFLNGK
RIILNGGCLHHDNGCLGAAAYDRAEERKVELMKAAGFNAVRTSHNIPSEEFLHACDRLGLLVIDETFDGWRDSKNQYDYS
ILFDQWWQRDIESMVLRDRNHPSIFCWSIGNEVIERKKLEVVTTARKLADYVHKFDPSRPVTSALAAWDNDWEIYDPLAA
VHEIVGYNYLLHRAPVDHQRVPSRVIMQTESYPRDVFANWSLVNDHDYIIGDFVWTAIDYLGESGIGRFYYAGESEGEHY
QRNHYPWHGAYCGDIDLTGWRKPISHYRDLLYNPDKKLYLAVKEPDNYYGKVKETLWSVWPTWESWNWPGHEGKEIEVEI
YSRYPKVRLYLNDKLIGEQFTGQEQQFKAVFPISYEPGILKAVGVESEIEIEKTILQTAGKPVKIQLTADRTKIKANGQD
LSFITVEILDKEGILEPNADNQLTFEVKGAGTIVAVGNADLKDTDSYIGYQRKAWKGRAIVVVKSTRKEGKIMLKVASPG
LVPATVSIRTSK
>Mature_812_residues
MKKKKLPLVCWDSIKLLTALFLLWGVCFGADAYPESEYRKQLFDYDWKFKLGDYPDASLNTYDDADWRILDLPHDWSIEG
TLDPENPMGNDGGYFPAGTGWYRKSFEISSKYRGKKVGIYFEGVYMNSEVFVNGKSIGIRPYGYSSFYYDLTPYLRYDDK
NIIAVRVDNSQQKNCRWYTGTGIYRHVWLTVMNPIHIEHWGIAITTPEVSEERAVVQIKTILRNETSSNRQITLTTKLTK
GNDESGKGEIKVDLPANGIKEITQKISVLYPALWSPESPHLYNAHFLVTEAGDVIDSITESFGIRTVTYSAEQGLFLNGK
RIILNGGCLHHDNGCLGAAAYDRAEERKVELMKAAGFNAVRTSHNIPSEEFLHACDRLGLLVIDETFDGWRDSKNQYDYS
ILFDQWWQRDIESMVLRDRNHPSIFCWSIGNEVIERKKLEVVTTARKLADYVHKFDPSRPVTSALAAWDNDWEIYDPLAA
VHEIVGYNYLLHRAPVDHQRVPSRVIMQTESYPRDVFANWSLVNDHDYIIGDFVWTAIDYLGESGIGRFYYAGESEGEHY
QRNHYPWHGAYCGDIDLTGWRKPISHYRDLLYNPDKKLYLAVKEPDNYYGKVKETLWSVWPTWESWNWPGHEGKEIEVEI
YSRYPKVRLYLNDKLIGEQFTGQEQQFKAVFPISYEPGILKAVGVESEIEIEKTILQTAGKPVKIQLTADRTKIKANGQD
LSFITVEILDKEGILEPNADNQLTFEVKGAGTIVAVGNADLKDTDSYIGYQRKAWKGRAIVVVKSTRKEGKIMLKVASPG
LVPATVSIRTSK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=415, Percent_Identity=25.3012048192771, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI48994920, Length=515, Percent_Identity=26.2135922330097, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1786539, Length=458, Percent_Identity=27.7292576419214, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1787903, Length=411, Percent_Identity=24.5742092457421, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17510825, Length=398, Percent_Identity=26.1306532663317, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922582, Length=406, Percent_Identity=25.615763546798, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI62471735, Length=408, Percent_Identity=25.4901960784314, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655438, Length=408, Percent_Identity=25.4901960784314, Blast_Score=105, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24651745, Length=424, Percent_Identity=24.0566037735849, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 92967; Mature: 92967

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKLPLVCWDSIKLLTALFLLWGVCFGADAYPESEYRKQLFDYDWKFKLGDYPDASLN
CCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCC
TYDDADWRILDLPHDWSIEGTLDPENPMGNDGGYFPAGTGWYRKSFEISSKYRGKKVGIY
CCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
FEGVYMNSEVFVNGKSIGIRPYGYSSFYYDLTPYLRYDDKNIIAVRVDNSQQKNCRWYTG
EEEEEECCEEEEECCEEEEECCCCCCEEEECCCEEEECCCCEEEEEECCCCCCCCEEEEC
TGIYRHVWLTVMNPIHIEHWGIAITTPEVSEERAVVQIKTILRNETSSNRQITLTTKLTK
CCCEEEEEEEECCCEEEEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCCCEEEEEEEEEC
GNDESGKGEIKVDLPANGIKEITQKISVLYPALWSPESPHLYNAHFLVTEAGDVIDSITE
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHHHHHH
SFGIRTVTYSAEQGLFLNGKRIILNGGCLHHDNGCLGAAAYDRAEERKVELMKAAGFNAV
HCCEEEEEEECCCCEEECCCEEEEECCEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCE
RTSHNIPSEEFLHACDRLGLLVIDETFDGWRDSKNQYDYSILFDQWWQRDIESMVLRDRN
ECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
HPSIFCWSIGNEVIERKKLEVVTTARKLADYVHKFDPSRPVTSALAAWDNDWEIYDPLAA
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEHHHHHH
VHEIVGYNYLLHRAPVDHQRVPSRVIMQTESYPRDVFANWSLVNDHDYIIGDFVWTAIDY
HHHHHCCCEEEECCCCCHHHCCCCEEEECCCCCCHHHCCCEEECCCCEEEHHHHHHHHHH
LGESGIGRFYYAGESEGEHYQRNHYPWHGAYCGDIDLTGWRKPISHYRDLLYNPDKKLYL
HCCCCCCEEEECCCCCCCCHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEE
AVKEPDNYYGKVKETLWSVWPTWESWNWPGHEGKEIEVEIYSRYPKVRLYLNDKLIGEQF
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEECCCCC
TGQEQQFKAVFPISYEPGILKAVGVESEIEIEKTILQTAGKPVKIQLTADRTKIKANGQD
CCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCC
LSFITVEILDKEGILEPNADNQLTFEVKGAGTIVAVGNADLKDTDSYIGYQRKAWKGRAI
EEEEEEEEECCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHCCCCHHCCCCCEE
VVVKSTRKEGKIMLKVASPGLVPATVSIRTSK
EEEECCCCCCEEEEEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MKKKKLPLVCWDSIKLLTALFLLWGVCFGADAYPESEYRKQLFDYDWKFKLGDYPDASLN
CCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCC
TYDDADWRILDLPHDWSIEGTLDPENPMGNDGGYFPAGTGWYRKSFEISSKYRGKKVGIY
CCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
FEGVYMNSEVFVNGKSIGIRPYGYSSFYYDLTPYLRYDDKNIIAVRVDNSQQKNCRWYTG
EEEEEECCEEEEECCEEEEECCCCCCEEEECCCEEEECCCCEEEEEECCCCCCCCEEEEC
TGIYRHVWLTVMNPIHIEHWGIAITTPEVSEERAVVQIKTILRNETSSNRQITLTTKLTK
CCCEEEEEEEECCCEEEEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCCCEEEEEEEEEC
GNDESGKGEIKVDLPANGIKEITQKISVLYPALWSPESPHLYNAHFLVTEAGDVIDSITE
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHHHHHH
SFGIRTVTYSAEQGLFLNGKRIILNGGCLHHDNGCLGAAAYDRAEERKVELMKAAGFNAV
HCCEEEEEEECCCCEEECCCEEEEECCEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCE
RTSHNIPSEEFLHACDRLGLLVIDETFDGWRDSKNQYDYSILFDQWWQRDIESMVLRDRN
ECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
HPSIFCWSIGNEVIERKKLEVVTTARKLADYVHKFDPSRPVTSALAAWDNDWEIYDPLAA
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEHHHHHH
VHEIVGYNYLLHRAPVDHQRVPSRVIMQTESYPRDVFANWSLVNDHDYIIGDFVWTAIDY
HHHHHCCCEEEECCCCCHHHCCCCEEEECCCCCCHHHCCCEEECCCCEEEHHHHHHHHHH
LGESGIGRFYYAGESEGEHYQRNHYPWHGAYCGDIDLTGWRKPISHYRDLLYNPDKKLYL
HCCCCCCEEEECCCCCCCCHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEE
AVKEPDNYYGKVKETLWSVWPTWESWNWPGHEGKEIEVEIYSRYPKVRLYLNDKLIGEQF
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEECCCCC
TGQEQQFKAVFPISYEPGILKAVGVESEIEIEKTILQTAGKPVKIQLTADRTKIKANGQD
CCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCC
LSFITVEILDKEGILEPNADNQLTFEVKGAGTIVAVGNADLKDTDSYIGYQRKAWKGRAI
EEEEEEEEECCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHCCCCHHCCCCCEE
VVVKSTRKEGKIMLKVASPGLVPATVSIRTSK
EEEECCCCCCEEEEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA