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The map label for this gene is pdxB [H]

Identifier: 150003629

GI number: 150003629

Start: 1411767

End: 1412792

Strand: Reverse

Name: pdxB [H]

Synonym: BVU_1060

Alternate gene names: 150003629

Gene position: 1412792-1411767 (Counterclockwise)

Preceding gene: 150003639

Following gene: 150003622

Centisome position: 27.36

GC content: 46.1

Gene sequence:

>1026_bases
ATGAAAATAATTGTAGATAATAAAATACCTTATATACATGAAGCTGTCGAACAGATAGCCGATGAAGTGGTGTATTTGCC
CGGCAGTGGTTTTACCGCCGGGGATGTACGGGATGCTGACGCATTGGTGATCCGTACCCGTACCCGTTGTAACCGTGAAC
TGCTGGAAGGAAGCAAGGTGAAATTCATTGCTACTGCGACCATCGGTTTTGATCATATTGATGTGGACTATTGTGATGAG
GCGGGAATTGTATGGAAAAATTGTCCCGGTTGTAATGCCGGCAGTGTGGAGCAGTACCTGCATTCGGTATTATTATTGTT
GAAACGCCGGAAGGGGGTGAGATTGGAAGAAAGCTGTCTGGGGATTGTTGGGGTAGGACATGTGGGAAGCCGTATCCAAC
GTATGGCGGAGGCATTGGGGATGCGGGTCTTGCTGAATGATCCTCCACGTGCGGACCGGGGGGAAACGGAATTTGTGGAT
TTGTCTGTACTGGCAAGGGAATGTGATATAATAACCTTTCATACTCCGCTGAACAGGAATGGGAAATATAAAACGTTCCA
TCTGGCGGATGCGGATTTCTTTGCCGGTTTGCAACGGAAACCTTTTATTGTGAATACTTCTCGTGGAGAAGTAATAGAGA
CATTGGCTTTGCTTGATGCGCTGAAGACAGGCCGGATACGTGATGCGGTTATTGATACGTGGGAAAATGAACCGGATATT
CACCCGGATTTGTTGCAGAAAGTTTTTTTGGGAACCCCTCATATAGCAGGGTATTCAGCTGACGGAAAGTCGAATGCTAC
ACGGATGGCATTGGAGGAGCTTTGTAACTTCTTCCATATACAGGCTGATTTTAAAATAGTACCGCCCGCTTTGCCGTATA
TGGATTATTCTTCTGATCCGGAAGAGGCTTTTCTACAGGTGTATGATCCGACTCGTGACAGTGATGCATTGAAACGGCAT
CCCGAAGAATTTGAACATTTGCGGGGGAATTATCCATTGAGGCGTGAAATTTCTTTTTTACCGTAG

Upstream 100 bases:

>100_bases
GCTGTTTTATTGAATGTACAAATCTATGCAAAGTTTTCTAATCAGCCAATTATTATTCGGTAAAAGTGTCTACCTTTGTT
CCCAAGCAAGTTTTTGAAAG

Downstream 100 bases:

>100_bases
GGGGACATTTCAATGTACCCCTACGGTGGCCAGAACTTTTAGTCTGCTTTTGCTGTTTGCATCTCTTCTGCTACAATCTG
TTCGCAATCTTCAATGCTGT

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKIIVDNKIPYIHEAVEQIADEVVYLPGSGFTAGDVRDADALVIRTRTRCNRELLEGSKVKFIATATIGFDHIDVDYCDE
AGIVWKNCPGCNAGSVEQYLHSVLLLLKRRKGVRLEESCLGIVGVGHVGSRIQRMAEALGMRVLLNDPPRADRGETEFVD
LSVLARECDIITFHTPLNRNGKYKTFHLADADFFAGLQRKPFIVNTSRGEVIETLALLDALKTGRIRDAVIDTWENEPDI
HPDLLQKVFLGTPHIAGYSADGKSNATRMALEELCNFFHIQADFKIVPPALPYMDYSSDPEEAFLQVYDPTRDSDALKRH
PEEFEHLRGNYPLRREISFLP

Sequences:

>Translated_341_residues
MKIIVDNKIPYIHEAVEQIADEVVYLPGSGFTAGDVRDADALVIRTRTRCNRELLEGSKVKFIATATIGFDHIDVDYCDE
AGIVWKNCPGCNAGSVEQYLHSVLLLLKRRKGVRLEESCLGIVGVGHVGSRIQRMAEALGMRVLLNDPPRADRGETEFVD
LSVLARECDIITFHTPLNRNGKYKTFHLADADFFAGLQRKPFIVNTSRGEVIETLALLDALKTGRIRDAVIDTWENEPDI
HPDLLQKVFLGTPHIAGYSADGKSNATRMALEELCNFFHIQADFKIVPPALPYMDYSSDPEEAFLQVYDPTRDSDALKRH
PEEFEHLRGNYPLRREISFLP
>Mature_341_residues
MKIIVDNKIPYIHEAVEQIADEVVYLPGSGFTAGDVRDADALVIRTRTRCNRELLEGSKVKFIATATIGFDHIDVDYCDE
AGIVWKNCPGCNAGSVEQYLHSVLLLLKRRKGVRLEESCLGIVGVGHVGSRIQRMAEALGMRVLLNDPPRADRGETEFVD
LSVLARECDIITFHTPLNRNGKYKTFHLADADFFAGLQRKPFIVNTSRGEVIETLALLDALKTGRIRDAVIDTWENEPDI
HPDLLQKVFLGTPHIAGYSADGKSNATRMALEELCNFFHIQADFKIVPPALPYMDYSSDPEEAFLQVYDPTRDSDALKRH
PEEFEHLRGNYPLRREISFLP

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=254, Percent_Identity=24.0157480314961, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI61743967, Length=255, Percent_Identity=30.5882352941176, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI4557497, Length=255, Percent_Identity=30.5882352941176, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI6912396, Length=246, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI145580575, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788660, Length=353, Percent_Identity=41.3597733711048, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1787645, Length=285, Percent_Identity=28.0701754385965, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789279, Length=260, Percent_Identity=28.8461538461538, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87082289, Length=276, Percent_Identity=25.3623188405797, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=249, Percent_Identity=27.3092369477912, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=266, Percent_Identity=27.8195488721804, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=266, Percent_Identity=26.6917293233083, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=178, Percent_Identity=30.3370786516854, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324964, Length=278, Percent_Identity=21.5827338129496, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646446, Length=269, Percent_Identity=29.368029739777, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646448, Length=269, Percent_Identity=29.368029739777, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646452, Length=269, Percent_Identity=29.368029739777, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646450, Length=269, Percent_Identity=29.368029739777, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62472511, Length=269, Percent_Identity=29.368029739777, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921140, Length=280, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 38338; Mature: 38338

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVDNKIPYIHEAVEQIADEVVYLPGSGFTAGDVRDADALVIRTRTRCNRELLEGSKV
CEEEECCCCCHHHHHHHHHHHCEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCE
KFIATATIGFDHIDVDYCDEAGIVWKNCPGCNAGSVEQYLHSVLLLLKRRKGVRLEESCL
EEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHE
GIVGVGHVGSRIQRMAEALGMRVLLNDPPRADRGETEFVDLSVLARECDIITFHTPLNRN
EEEECCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEHHHHHHHHCCEEEEECCCCCC
GKYKTFHLADADFFAGLQRKPFIVNTSRGEVIETLALLDALKTGRIRDAVIDTWENEPDI
CCEEEEEECCHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
HPDLLQKVFLGTPHIAGYSADGKSNATRMALEELCNFFHIQADFKIVPPALPYMDYSSDP
CHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCEECCCCCCCCCCCCCH
EEAFLQVYDPTRDSDALKRHPEEFEHLRGNYPLRREISFLP
HHHHEEEECCCCCCHHHHCCCHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure
MKIIVDNKIPYIHEAVEQIADEVVYLPGSGFTAGDVRDADALVIRTRTRCNRELLEGSKV
CEEEECCCCCHHHHHHHHHHHCEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCE
KFIATATIGFDHIDVDYCDEAGIVWKNCPGCNAGSVEQYLHSVLLLLKRRKGVRLEESCL
EEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHE
GIVGVGHVGSRIQRMAEALGMRVLLNDPPRADRGETEFVDLSVLARECDIITFHTPLNRN
EEEECCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEHHHHHHHHCCEEEEECCCCCC
GKYKTFHLADADFFAGLQRKPFIVNTSRGEVIETLALLDALKTGRIRDAVIDTWENEPDI
CCEEEEEECCHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
HPDLLQKVFLGTPHIAGYSADGKSNATRMALEELCNFFHIQADFKIVPPALPYMDYSSDP
CHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCEECCCCCCCCCCCCCH
EEAFLQVYDPTRDSDALKRHPEEFEHLRGNYPLRREISFLP
HHHHEEEECCCCCCHHHHCCCHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA