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The map label for this gene is rfbD [C]

Identifier: 150003560

GI number: 150003560

Start: 1305776

End: 1306729

Strand: Direct

Name: rfbD [C]

Synonym: BVU_0989

Alternate gene names: 150003560

Gene position: 1305776-1306729 (Clockwise)

Preceding gene: 150003556

Following gene: 150003561

Centisome position: 25.29

GC content: 43.71

Gene sequence:

>954_bases
ATGAAAAATGTATTAATTATCGGCTCCACCGGTCAGATCGGTTCGGAACTAACCATGAAATTGAGAAGTATTTACAATGG
TAATATCGTAGCCGGATATATTCCCGGTGCTGAGCCTAAAGGGGAATTAAAAGAATCAGGCCCTTCTGCCATCGTAGATA
TCACCAACGAACAACAAATTGCCGAAACTGTATCAAAGTACAACATCGACACCATTTACAACCTGGCAGCCTTACTATCG
GCTGTAGCAGAAGCCAAACCTCAACTAGCATGGAAAATAGGCATGGGAGGCCTGTTCAACGTATTGGAAGTAGCACGCGA
AATGGGTTGCGCCGTATTTACCCCAAGTTCTATCGGTGTCTTCGGCAACAACACCCCTAAAGACAAGACCCCGCAAGACA
CTATCCGTAATCCGCGTACCATGTATGGCGTTACAAAAGTATCAGGAGAATTACTGAGCGATTATTACCATATCCGTTTT
GGCGTAGATACCCGCTCCGTTCGTTTCCCCGGATTAATTTCGTATGTCACACCTCCGGGTGGCGGGACCACTGATTATGC
TGTAGACATCTATTATTCGGCTGTAAAAGGTGAAAAATTTGAATGTCCTATTGCAGCCGGTACATTCATGGATATGATGT
ATATGCCTGACGGTTTACGTGCAGCCATTGAAATCATGGAAGCGAATCCCGACAAACTAATTCATCGCAATTCGTTCAAC
ATTGCTTCAATGAGTTTTGATCCGGAAATTATTTATAACAATATAAAGAAGTATATGCCCGACTTCCATATGGAGTACAA
AGTAGATCCGCTACGTCAAGCTATCGCCGAATCATGGCCGAACTCACTGGACGACACTTGTGCCCGTGAGGAATGGGGAT
GGAAACCGGAATATGATCTAGACAGCATGACACAGGATATGCTTGCCAAACTAAAAATACGATTCAATAAATAA

Upstream 100 bases:

>100_bases
TTTTTGATATCCGATTAAATTATAGAGAAAATTTTGATAAAATATGCAGTTTTCTATCTATCTTTGCTTCCAAAAGAAAA
CTTAAAGCAAATAGCAAACT

Downstream 100 bases:

>100_bases
ACCACTAATATTGAATAAATAAAATAGCCTTCTCTATTCTATATGGAAAAGATAGAGAAGGCTATTTTTATACATTTCTT
GCACTTATATAAAATTTTGA

Product: NAD dependent nucleotide-diphosphate-sugar epimerase

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKNVLIIGSTGQIGSELTMKLRSIYNGNIVAGYIPGAEPKGELKESGPSAIVDITNEQQIAETVSKYNIDTIYNLAALLS
AVAEAKPQLAWKIGMGGLFNVLEVAREMGCAVFTPSSIGVFGNNTPKDKTPQDTIRNPRTMYGVTKVSGELLSDYYHIRF
GVDTRSVRFPGLISYVTPPGGGTTDYAVDIYYSAVKGEKFECPIAAGTFMDMMYMPDGLRAAIEIMEANPDKLIHRNSFN
IASMSFDPEIIYNNIKKYMPDFHMEYKVDPLRQAIAESWPNSLDDTCAREEWGWKPEYDLDSMTQDMLAKLKIRFNK

Sequences:

>Translated_317_residues
MKNVLIIGSTGQIGSELTMKLRSIYNGNIVAGYIPGAEPKGELKESGPSAIVDITNEQQIAETVSKYNIDTIYNLAALLS
AVAEAKPQLAWKIGMGGLFNVLEVAREMGCAVFTPSSIGVFGNNTPKDKTPQDTIRNPRTMYGVTKVSGELLSDYYHIRF
GVDTRSVRFPGLISYVTPPGGGTTDYAVDIYYSAVKGEKFECPIAAGTFMDMMYMPDGLRAAIEIMEANPDKLIHRNSFN
IASMSFDPEIIYNNIKKYMPDFHMEYKVDPLRQAIAESWPNSLDDTCAREEWGWKPEYDLDSMTQDMLAKLKIRFNK
>Mature_317_residues
MKNVLIIGSTGQIGSELTMKLRSIYNGNIVAGYIPGAEPKGELKESGPSAIVDITNEQQIAETVSKYNIDTIYNLAALLS
AVAEAKPQLAWKIGMGGLFNVLEVAREMGCAVFTPSSIGVFGNNTPKDKTPQDTIRNPRTMYGVTKVSGELLSDYYHIRF
GVDTRSVRFPGLISYVTPPGGGTTDYAVDIYYSAVKGEKFECPIAAGTFMDMMYMPDGLRAAIEIMEANPDKLIHRNSFN
IASMSFDPEIIYNNIKKYMPDFHMEYKVDPLRQAIAESWPNSLDDTCAREEWGWKPEYDLDSMTQDMLAKLKIRFNK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=309, Percent_Identity=43.6893203883495, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24667531, Length=311, Percent_Identity=39.871382636656, Blast_Score=252, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 35268; Mature: 35268

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVLIIGSTGQIGSELTMKLRSIYNGNIVAGYIPGAEPKGELKESGPSAIVDITNEQQI
CCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCHHHH
AETVSKYNIDTIYNLAALLSAVAEAKPQLAWKIGMGGLFNVLEVAREMGCAVFTPSSIGV
HHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEECCCCCCC
FGNNTPKDKTPQDTIRNPRTMYGVTKVSGELLSDYYHIRFGVDTRSVRFPGLISYVTPPG
CCCCCCCCCCCHHHHCCCHHEEEHHHHHHHHHHCEEEEEECCCCCCEECCCEEEEECCCC
GGTTDYAVDIYYSAVKGEKFECPIAAGTFMDMMYMPDGLRAAIEIMEANPDKLIHRNSFN
CCCCCEEEEEEEEECCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHEEECCCCC
IASMSFDPEIIYNNIKKYMPDFHMEYKVDPLRQAIAESWPNSLDDTCAREEWGWKPEYDL
EEEECCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
DSMTQDMLAKLKIRFNK
HHHHHHHHHHHHEEECC
>Mature Secondary Structure
MKNVLIIGSTGQIGSELTMKLRSIYNGNIVAGYIPGAEPKGELKESGPSAIVDITNEQQI
CCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCHHHH
AETVSKYNIDTIYNLAALLSAVAEAKPQLAWKIGMGGLFNVLEVAREMGCAVFTPSSIGV
HHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEECCCCCCC
FGNNTPKDKTPQDTIRNPRTMYGVTKVSGELLSDYYHIRFGVDTRSVRFPGLISYVTPPG
CCCCCCCCCCCHHHHCCCHHEEEHHHHHHHHHHCEEEEEECCCCCCEECCCEEEEECCCC
GGTTDYAVDIYYSAVKGEKFECPIAAGTFMDMMYMPDGLRAAIEIMEANPDKLIHRNSFN
CCCCCEEEEEEEEECCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHEEECCCCC
IASMSFDPEIIYNNIKKYMPDFHMEYKVDPLRQAIAESWPNSLDDTCAREEWGWKPEYDL
EEEECCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
DSMTQDMLAKLKIRFNK
HHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA