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The map label for this gene is hemK [H]

Identifier: 150003436

GI number: 150003436

Start: 1165461

End: 1166297

Strand: Reverse

Name: hemK [H]

Synonym: BVU_0863

Alternate gene names: 150003436

Gene position: 1166297-1165461 (Counterclockwise)

Preceding gene: 150003446

Following gene: 150003435

Centisome position: 22.59

GC content: 42.29

Gene sequence:

>837_bases
ATGAATCCTGTTGTATCTTACATAAAACAAACCTTGCAGGGATATTACCCTGATTCAGAACTTGTGCCTATGGCTAAATT
GCTGCTTACACAAGTCTTCGGCATGTCTGTTGTTGAATTGTATGCAGGCAAAGATACTACTTTTTCGGTGAATGAGCGGA
AACAGCTGGATGATATTCTGGTTCGTCTGCAAAAACTGGAACCTATTCAATACATAATAGGTACGGAAGAATTTTATGGC
CTTACTTTTGAAGTGAATAAACATGTATTGATTCCACGTCCGGAAACAGGGGAGTTGGTAGACTGGATTATACGGGAGCA
TAAGTATGGTCGGGTTAGAATATTGGATATTGGTACGGGGAGCGGCTGCATCGCTGTCTCTTTGGCTAAGAATTTGGAAG
AGGCGGAGGTCGTTTCATGGGATGTTTCAGAAAAGGCGTTGCAAGTTGCGGAACGTAATTGCAGGCGGAACGGCGTTCGT
GTAATTCTTGAGCAAAGGGATGTGCTGCTTGCTTCTCCTGCAGGTGAGCGGTTTGATGTCATTGTCAGCAATCCACCTTA
TATTACAGAAAAGGAGAGGGCGGATATGAGTGCTAATGTCTTGGAATGGGAGCCGGAATTGGCTTTGTTTGTGCCGGATG
ATAGTCCGTTGCTGTTTTATCGGAAAATAGCAGAATTGGGTAGGGATATGCTAGTGTCTGGTGGAAGGCTTTATTTTGAA
ATTAATCAGGAATACGGTCGGGAAACGGTGGATATGTTGGCTGGTTTGGAATACAAAAATATAAAATTGAGAAAAGATCT
CTTTCAAAATGATAGAATGATAAAAGCGGAGAAATAA

Upstream 100 bases:

>100_bases
AGTCCGTTGTATGCCAGTTGCAGGCAACGGGAAATATATTTTTCATCTTTTGTCATAACGATCTTGTTTAAATTCTTACT
TTTGCATATAAAATGACACT

Downstream 100 bases:

>100_bases
GATGAAGGGAGAATTAAGTGAGAATGATTTGAGGTATAAGGCAGAAGCTTATTGCTCTTTAATGGAGCGTTGTGTAGCAG
ATGTAGAGGCTAAATTATCA

Product: putative protoporphyrinogen oxidase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNPVVSYIKQTLQGYYPDSELVPMAKLLLTQVFGMSVVELYAGKDTTFSVNERKQLDDILVRLQKLEPIQYIIGTEEFYG
LTFEVNKHVLIPRPETGELVDWIIREHKYGRVRILDIGTGSGCIAVSLAKNLEEAEVVSWDVSEKALQVAERNCRRNGVR
VILEQRDVLLASPAGERFDVIVSNPPYITEKERADMSANVLEWEPELALFVPDDSPLLFYRKIAELGRDMLVSGGRLYFE
INQEYGRETVDMLAGLEYKNIKLRKDLFQNDRMIKAEK

Sequences:

>Translated_278_residues
MNPVVSYIKQTLQGYYPDSELVPMAKLLLTQVFGMSVVELYAGKDTTFSVNERKQLDDILVRLQKLEPIQYIIGTEEFYG
LTFEVNKHVLIPRPETGELVDWIIREHKYGRVRILDIGTGSGCIAVSLAKNLEEAEVVSWDVSEKALQVAERNCRRNGVR
VILEQRDVLLASPAGERFDVIVSNPPYITEKERADMSANVLEWEPELALFVPDDSPLLFYRKIAELGRDMLVSGGRLYFE
INQEYGRETVDMLAGLEYKNIKLRKDLFQNDRMIKAEK
>Mature_278_residues
MNPVVSYIKQTLQGYYPDSELVPMAKLLLTQVFGMSVVELYAGKDTTFSVNERKQLDDILVRLQKLEPIQYIIGTEEFYG
LTFEVNKHVLIPRPETGELVDWIIREHKYGRVRILDIGTGSGCIAVSLAKNLEEAEVVSWDVSEKALQVAERNCRRNGVR
VILEQRDVLLASPAGERFDVIVSNPPYITEKERADMSANVLEWEPELALFVPDDSPLLFYRKIAELGRDMLVSGGRLYFE
INQEYGRETVDMLAGLEYKNIKLRKDLFQNDRMIKAEK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=235, Percent_Identity=28.0851063829787, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1787463, Length=249, Percent_Identity=32.9317269076305, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87082085, Length=202, Percent_Identity=31.6831683168317, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324266, Length=154, Percent_Identity=33.1168831168831, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582226, Length=223, Percent_Identity=28.6995515695067, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR022438
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31850; Mature: 31850

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVVSYIKQTLQGYYPDSELVPMAKLLLTQVFGMSVVELYAGKDTTFSVNERKQLDDIL
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHH
VRLQKLEPIQYIIGTEEFYGLTFEVNKHVLIPRPETGELVDWIIREHKYGRVRILDIGTG
HHHHHCCCCCEEECCHHHEEEEEEECCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCC
SGCIAVSLAKNLEEAEVVSWDVSEKALQVAERNCRRNGVRVILEQRDVLLASPAGERFDV
CCEEEEEECCCCCHHHEEEECHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCEEE
IVSNPPYITEKERADMSANVLEWEPELALFVPDDSPLLFYRKIAELGRDMLVSGGRLYFE
EECCCCCCCCHHHCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCHHHEECCCEEEEE
INQEYGRETVDMLAGLEYKNIKLRKDLFQNDRMIKAEK
ECHHHCHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MNPVVSYIKQTLQGYYPDSELVPMAKLLLTQVFGMSVVELYAGKDTTFSVNERKQLDDIL
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHH
VRLQKLEPIQYIIGTEEFYGLTFEVNKHVLIPRPETGELVDWIIREHKYGRVRILDIGTG
HHHHHCCCCCEEECCHHHEEEEEEECCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCC
SGCIAVSLAKNLEEAEVVSWDVSEKALQVAERNCRRNGVRVILEQRDVLLASPAGERFDV
CCEEEEEECCCCCHHHEEEECHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCEEE
IVSNPPYITEKERADMSANVLEWEPELALFVPDDSPLLFYRKIAELGRDMLVSGGRLYFE
EECCCCCCCCHHHCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCHHHEECCCEEEEE
INQEYGRETVDMLAGLEYKNIKLRKDLFQNDRMIKAEK
ECHHHCHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA