Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 150003352

GI number: 150003352

Start: 1077919

End: 1079196

Strand: Direct

Name: mdeA [H]

Synonym: BVU_0770

Alternate gene names: 150003352

Gene position: 1077919-1079196 (Clockwise)

Preceding gene: 150003351

Following gene: 150003353

Centisome position: 20.88

GC content: 51.96

Gene sequence:

>1278_bases
ATGGATAAGAAGTATAGCAGGGAGACCTTATGCGTACAGGCAGGCTGGACTCCGAAAAAAGGCGAACCGCGCGTTTTGCC
TATTTATCAAAGTACCACATTCAAGTATGACACCAGCGAACAGATGGCGCGTCTGTTCGATCTGGAAGATTCGGGATATT
TCTATACACGCTTGCAAAACCCCACCAATGATGCCGTCGCCGCAAAAATCGCAGCATTGGAAGGTGGTGTGGGTGCTATG
CTGACCTCGTCCGGCCAGGCTGCCAACTTCTATGCCATTTTCAATATCTGTCAGGCAGGCGACCACTTCGTATGCTCGTC
TACCATCTACGGAGGTACATTCAATCTGTTTGGCGTCACGCTGAAAAAACTGGGCATCGAGTGTACCTTCATTGACGCCA
ATGCATCCGAGGAGGAAATAGACAAGGCTTTCCGCCCGAATACCAAAGCGTTGTTCGGCGAAACCATCTCCAATCCGAGC
ATCGACGTGCTGGACATCGAAAAGTTCGCACGTATCGCACACAAACACGGTGTGCCTTTGATTGTGGACAACACCTTCGC
CACTCCCATCAACTGCCGCCCGTTTGAATGGGGAGCGGATATCGTCACCCATTCCACAACCAAATACATGGACGGTCATG
CCACCAGTGTAGGAGGCGCCATTGTGGACAGCGGCAACTTTGACTGGGAAGCCCATGCGGACAAATTCCCCGGTCTGACC
CAGCCCGACGAATCTTATCACGGACTGACTTATACCAAAGCGTTCGGCAAAATGGCTTACATGACGAAAGCAACCGCCCA
ACTGATGCGTGACCTCGGCTCCATCCAGTCTCCGCAAAATGCGTTCCTGCTGAATCTGGGATTGGAAACATTGCACCTGC
GCGTGCCCCGTCATTGCGAAAACGCACAGAAAGTGGCCGAATGGCTGGAAGCCAACCCGAAAGTGAAATGGGTGAATTAT
TGCGGACTGAAAAGCAGCAAATACTATGATCTGGCACAGAAATACATGCCAAACGGCTCGTGCGGTGTCATCGCTTTCGG
CTTGAACGGAACCCGTGAGGAAGCGATAGAATTTATGGACAGGCTGAAATTCATCTGCATCGTAACCCATGTGGCCGACG
CACGTACCTGCGTACTGCATCCCGCCAGCCATACTCACCGCCAGCTCACCGACGAGCAGTTGCGCGAAGCGGGAGTCGCT
CCGGACCTGATCCGTTTGTCGGTAGGTATTGAGAATGTAAGCGACATCATTGCCGATATTGAACAAGCTTTACAATAA

Upstream 100 bases:

>100_bases
CAAATATAACACTTTTCCCAGTAATTGAACCCTAAATGACAGATTATTCTTAATTTTGCTGCGAAATAAATGAATATGTA
ATTTTAATAACAATATAAAC

Downstream 100 bases:

>100_bases
GGCGAAGCGGATGAAAAGAATCATTATCATTGGCGCCACTTCCGGCATCGGACTGGAAGTGGCGCGATGCTATCTGAAAG
CCGGCTGGCAGGTAGGCGTG

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MDKKYSRETLCVQAGWTPKKGEPRVLPIYQSTTFKYDTSEQMARLFDLEDSGYFYTRLQNPTNDAVAAKIAALEGGVGAM
LTSSGQAANFYAIFNICQAGDHFVCSSTIYGGTFNLFGVTLKKLGIECTFIDANASEEEIDKAFRPNTKALFGETISNPS
IDVLDIEKFARIAHKHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHATSVGGAIVDSGNFDWEAHADKFPGLT
QPDESYHGLTYTKAFGKMAYMTKATAQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCENAQKVAEWLEANPKVKWVNY
CGLKSSKYYDLAQKYMPNGSCGVIAFGLNGTREEAIEFMDRLKFICIVTHVADARTCVLHPASHTHRQLTDEQLREAGVA
PDLIRLSVGIENVSDIIADIEQALQ

Sequences:

>Translated_425_residues
MDKKYSRETLCVQAGWTPKKGEPRVLPIYQSTTFKYDTSEQMARLFDLEDSGYFYTRLQNPTNDAVAAKIAALEGGVGAM
LTSSGQAANFYAIFNICQAGDHFVCSSTIYGGTFNLFGVTLKKLGIECTFIDANASEEEIDKAFRPNTKALFGETISNPS
IDVLDIEKFARIAHKHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHATSVGGAIVDSGNFDWEAHADKFPGLT
QPDESYHGLTYTKAFGKMAYMTKATAQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCENAQKVAEWLEANPKVKWVNY
CGLKSSKYYDLAQKYMPNGSCGVIAFGLNGTREEAIEFMDRLKFICIVTHVADARTCVLHPASHTHRQLTDEQLREAGVA
PDLIRLSVGIENVSDIIADIEQALQ
>Mature_425_residues
MDKKYSRETLCVQAGWTPKKGEPRVLPIYQSTTFKYDTSEQMARLFDLEDSGYFYTRLQNPTNDAVAAKIAALEGGVGAM
LTSSGQAANFYAIFNICQAGDHFVCSSTIYGGTFNLFGVTLKKLGIECTFIDANASEEEIDKAFRPNTKALFGETISNPS
IDVLDIEKFARIAHKHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHATSVGGAIVDSGNFDWEAHADKFPGLT
QPDESYHGLTYTKAFGKMAYMTKATAQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCENAQKVAEWLEANPKVKWVNY
CGLKSSKYYDLAQKYMPNGSCGVIAFGLNGTREEAIEFMDRLKFICIVTHVADARTCVLHPASHTHRQLTDEQLREAGVA
PDLIRLSVGIENVSDIIADIEQALQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=420, Percent_Identity=33.5714285714286, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI299473759, Length=420, Percent_Identity=30.952380952381, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI25453487, Length=419, Percent_Identity=28.1622911694511, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1790375, Length=401, Percent_Identity=28.1795511221945, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=26.2295081967213, Blast_Score=121, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=35.2941176470588, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17538055, Length=422, Percent_Identity=33.4123222748815, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=33.6492890995261, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=413, Percent_Identity=46.2469733656174, Blast_Score=355, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6319307, Length=405, Percent_Identity=32.3456790123457, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=293, Percent_Identity=28.3276450511945, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321254, Length=441, Percent_Identity=24.7165532879819, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=415, Percent_Identity=26.7469879518072, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=32.0665083135392, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46993; Mature: 46993

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKYSRETLCVQAGWTPKKGEPRVLPIYQSTTFKYDTSEQMARLFDLEDSGYFYTRLQN
CCCCCCCCEEEEECCCCCCCCCCCEEEEEECCEEEECCHHHHHHHHCCCCCCEEEEEECC
PTNDAVAAKIAALEGGVGAMLTSSGQAANFYAIFNICQAGDHFVCSSTIYGGTFNLFGVT
CCCHHHHHHHHHHCCCCCEEEECCCCCCEEHEEEHHHHCCCCEEEECEECCCCHHHHHHH
LKKLGIECTFIDANASEEEIDKAFRPNTKALFGETISNPSIDVLDIEKFARIAHKHGVPL
HHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCE
IVDNTFATPINCRPFEWGADIVTHSTTKYMDGHATSVGGAIVDSGNFDWEAHADKFPGLT
EECCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
QPDESYHGLTYTKAFGKMAYMTKATAQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCHHHH
NAQKVAEWLEANPKVKWVNYCGLKSSKYYDLAQKYMPNGSCGVIAFGLNGTREEAIEFMD
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHH
RLKFICIVTHVADARTCVLHPASHTHRQLTDEQLREAGVAPDLIRLSVGIENVSDIIADI
HHHHHHHEEHHCCCCEEEECCCCHHHHHCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHH
EQALQ
HHHCC
>Mature Secondary Structure
MDKKYSRETLCVQAGWTPKKGEPRVLPIYQSTTFKYDTSEQMARLFDLEDSGYFYTRLQN
CCCCCCCCEEEEECCCCCCCCCCCEEEEEECCEEEECCHHHHHHHHCCCCCCEEEEEECC
PTNDAVAAKIAALEGGVGAMLTSSGQAANFYAIFNICQAGDHFVCSSTIYGGTFNLFGVT
CCCHHHHHHHHHHCCCCCEEEECCCCCCEEHEEEHHHHCCCCEEEECEECCCCHHHHHHH
LKKLGIECTFIDANASEEEIDKAFRPNTKALFGETISNPSIDVLDIEKFARIAHKHGVPL
HHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCE
IVDNTFATPINCRPFEWGADIVTHSTTKYMDGHATSVGGAIVDSGNFDWEAHADKFPGLT
EECCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
QPDESYHGLTYTKAFGKMAYMTKATAQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCHHHH
NAQKVAEWLEANPKVKWVNYCGLKSSKYYDLAQKYMPNGSCGVIAFGLNGTREEAIEFMD
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHH
RLKFICIVTHVADARTCVLHPASHTHRQLTDEQLREAGVAPDLIRLSVGIENVSDIIADI
HHHHHHHEEHHCCCCEEEECCCCHHHHHCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHH
EQALQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]