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The map label for this gene is topB [H]

Identifier: 150003293

GI number: 150003293

Start: 988236

End: 989996

Strand: Direct

Name: topB [H]

Synonym: BVU_0709

Alternate gene names: 150003293

Gene position: 988236-989996 (Clockwise)

Preceding gene: 150003292

Following gene: 150003294

Centisome position: 19.14

GC content: 55.59

Gene sequence:

>1761_bases
ATGATCGCAATAGTGACTGACAGGCCCAACGTGGGCAGAGAGATAGCAAGAGTGTTGGGAGCCGGCAGAAAAGAGAACGG
TTATATGACCGGGAACGGCTACATGGTGACATGGACATACGGCAATATGCTTTCCCTTGCAATGCCGAAGGATTCAGGAA
CAGCCCATGTGGAGTGGAAGGACTTTCCTCTGCTGCCGCCTTCCTTCCTGACAGTAAGGCACGTGAAGACGGATACCGGG
TGGAATCCCGACATCAATGCGGTGCTCCAGTTGAAAGTGATTGCCAAGGTGCTCAACGCATGCGACACCATTATCGCGGC
AACCGATGCCTCCCGAGAGGGAGAGATGCTGTTCCGCCATCTTTACGGTTTTCTGGGATGTAAACAACCCTGCCTCCGCC
TCTGGATCTCGTCTCTGACGGACGAGGCCATCACGGAAGGTATGGCGAACCTCCTTCCATGTGATCGGTTCGACAACCTG
TTCCTTGCGGCGGACAGCCGTAACCGGGCGGACTGGCTCCTGGGGGTCAATTCAAGCTATGCCATCTGTAAGGCGGTAAG
CTTCGGGAACAATTCGCTGGGCAGGGTACAGACCCCCGTACTGGCGGAGATATGCAGACGGTACCGGGAGAGGGAGAATT
TCCTGCCAGCCGACAGCTGGCCGGTATTCGTCAGTCTCTGCAAGGACCGACAAATCATAAAACTGCGCCACACGGAAGAT
TTTCAGGAGTACCGGTACGCGTCAGAACTGTACGGTGACTGCAAGGCGGTACGGAACGCACGTATCGCGACGGTCAGCCG
CGAGAGCGAGGACATCCGGGCACCGGCTCCCTACAACCTGACCGAACTTCAGAAGGACGCGAACCGTTACCACAACCTGA
CAGCCATACAGGTACAGGATATCACCCAAGGCCTGTACGAAAAGAAACTGATCTCCTATCCGCGTACCGCCAGCCGCCTG
CTGACAAGGGACGTATATGACACGTTGCCCGCTGTCATGGAAAAGATTCTTTCCCGCAAGGAATTCCGCCAATACGCCAA
AATGACGGACTTTGCCGCGCCGCCAAGCGACATCAGCGCACAGGAGACGACAGATCACCACGCGATCATTGTCACCGGCA
TGCCCCCCGGAACATTGAACAAGGAGGAACAACTGGTCTATACGCTCATTCTCGGCAGGACACTCGAAGCCTTCATGCCA
TCCTGCCGGGTGGAATATACGACCATCGACGTAGTATGTGCCGCACGGAAGTTCAGCGTCCAAACGTACCGCGTCCTGAA
AAAGGGGTGGCTCAGCATCTTCGGGCGGGAACATCTTATCGCAAGGGGGGGTATGCCCTGTTCCGCACTACCGGATTTCT
CCCCGGGAGAGCCGGTACCGGTAAGCGGATGCAATATCGTCCGCAAGAGAACACTTCCGGTATCCCCCTATACGGATGAG
GAGCTGGTCGGCTATATGGACCGGAGCGGATTAGGGACCGTCTCCACGCGTACCAATATCATCCGCACACTGCTGGAGCG
TAAATATATCCGCTATTCAGGCAAATATGTCATCCCCACCCCAAAAGGGCTTTTCTTCTATGAAACGGTACGTGGGATGA
AAGTGGCCGACACCTCCCTCACTTCCGGATGGGAAGCGGAAGTGGCACGGATAGAACAGGGAAAACTCACGCAAGAGGAG
TTCCTGGGAGGGGTACTGGAACAGGTGAAGGAGGTGACCGGTGAGATATTCCGGATCTATCAGGCTAAAAACAACCCGTA
G

Upstream 100 bases:

>100_bases
CGTCAGGAGGAATCTCCAAAGAAATAACAAAAAAATAAAATAAGTTTAACCTGTCCCTGTACTGCCCAATCCGGTACGGG
GCATCAAAAAGAAAAAGAAT

Downstream 100 bases:

>100_bases
GATATTACGCCACCGGCACGGGACGATTCTCAAAAGAGCAGGGAGTACAGGCTTTCAATGCCGTACTCCTTTTTTCATGG
GAGATTGGATAGTTTTTCCG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTAHVEWKDFPLLPPSFLTVRHVKTDTG
WNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRHLYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNL
FLAADSRNRADWLLGVNSSYAICKAVSFGNNSLGRVQTPVLAEICRRYRERENFLPADSWPVFVSLCKDRQIIKLRHTED
FQEYRYASELYGDCKAVRNARIATVSRESEDIRAPAPYNLTELQKDANRYHNLTAIQVQDITQGLYEKKLISYPRTASRL
LTRDVYDTLPAVMEKILSRKEFRQYAKMTDFAAPPSDISAQETTDHHAIIVTGMPPGTLNKEEQLVYTLILGRTLEAFMP
SCRVEYTTIDVVCAARKFSVQTYRVLKKGWLSIFGREHLIARGGMPCSALPDFSPGEPVPVSGCNIVRKRTLPVSPYTDE
ELVGYMDRSGLGTVSTRTNIIRTLLERKYIRYSGKYVIPTPKGLFFYETVRGMKVADTSLTSGWEAEVARIEQGKLTQEE
FLGGVLEQVKEVTGEIFRIYQAKNNP

Sequences:

>Translated_586_residues
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTAHVEWKDFPLLPPSFLTVRHVKTDTG
WNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRHLYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNL
FLAADSRNRADWLLGVNSSYAICKAVSFGNNSLGRVQTPVLAEICRRYRERENFLPADSWPVFVSLCKDRQIIKLRHTED
FQEYRYASELYGDCKAVRNARIATVSRESEDIRAPAPYNLTELQKDANRYHNLTAIQVQDITQGLYEKKLISYPRTASRL
LTRDVYDTLPAVMEKILSRKEFRQYAKMTDFAAPPSDISAQETTDHHAIIVTGMPPGTLNKEEQLVYTLILGRTLEAFMP
SCRVEYTTIDVVCAARKFSVQTYRVLKKGWLSIFGREHLIARGGMPCSALPDFSPGEPVPVSGCNIVRKRTLPVSPYTDE
ELVGYMDRSGLGTVSTRTNIIRTLLERKYIRYSGKYVIPTPKGLFFYETVRGMKVADTSLTSGWEAEVARIEQGKLTQEE
FLGGVLEQVKEVTGEIFRIYQAKNNP
>Mature_586_residues
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTAHVEWKDFPLLPPSFLTVRHVKTDTG
WNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRHLYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNL
FLAADSRNRADWLLGVNSSYAICKAVSFGNNSLGRVQTPVLAEICRRYRERENFLPADSWPVFVSLCKDRQIIKLRHTED
FQEYRYASELYGDCKAVRNARIATVSRESEDIRAPAPYNLTELQKDANRYHNLTAIQVQDITQGLYEKKLISYPRTASRL
LTRDVYDTLPAVMEKILSRKEFRQYAKMTDFAAPPSDISAQETTDHHAIIVTGMPPGTLNKEEQLVYTLILGRTLEAFMP
SCRVEYTTIDVVCAARKFSVQTYRVLKKGWLSIFGREHLIARGGMPCSALPDFSPGEPVPVSGCNIVRKRTLPVSPYTDE
ELVGYMDRSGLGTVSTRTNIIRTLLERKYIRYSGKYVIPTPKGLFFYETVRGMKVADTSLTSGWEAEVARIEQGKLTQEE
FLGGVLEQVKEVTGEIFRIYQAKNNP

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=500, Percent_Identity=21.4, Blast_Score=93, Evalue=7e-19,
Organism=Escherichia coli, GI1788061, Length=631, Percent_Identity=27.5752773375594, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI1787529, Length=439, Percent_Identity=23.2346241457859, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17555378, Length=620, Percent_Identity=22.9032258064516, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=573, Percent_Identity=22.1640488656195, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585251, Length=616, Percent_Identity=21.2662337662338, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 66061; Mature: 66061

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTAHVEWK
CEEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCEEEEEECCCCCEEEEEEC
DFPLLPPSFLTVRHVKTDTGWNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRH
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
LYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNLFLAADSRNRADWLLGVNSSY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCC
AICKAVSFGNNSLGRVQTPVLAEICRRYRERENFLPADSWPVFVSLCKDRQIIKLRHTED
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCHH
FQEYRYASELYGDCKAVRNARIATVSRESEDIRAPAPYNLTELQKDANRYHNLTAIQVQD
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEHHH
ITQGLYEKKLISYPRTASRLLTRDVYDTLPAVMEKILSRKEFRQYAKMTDFAAPPSDISA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QETTDHHAIIVTGMPPGTLNKEEQLVYTLILGRTLEAFMPSCRVEYTTIDVVCAARKFSV
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHH
QTYRVLKKGWLSIFGREHLIARGGMPCSALPDFSPGEPVPVSGCNIVRKRTLPVSPYTDE
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCH
ELVGYMDRSGLGTVSTRTNIIRTLLERKYIRYSGKYVIPTPKGLFFYETVRGMKVADTSL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEHHHCCCEEECCCC
TSGWEAEVARIEQGKLTQEEFLGGVLEQVKEVTGEIFRIYQAKNNP
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIAIVTDRPNVGREIARVLGAGRKENGYMTGNGYMVTWTYGNMLSLAMPKDSGTAHVEWK
CEEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCEEEEEECCCCCEEEEEEC
DFPLLPPSFLTVRHVKTDTGWNPDINAVLQLKVIAKVLNACDTIIAATDASREGEMLFRH
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
LYGFLGCKQPCLRLWISSLTDEAITEGMANLLPCDRFDNLFLAADSRNRADWLLGVNSSY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCC
AICKAVSFGNNSLGRVQTPVLAEICRRYRERENFLPADSWPVFVSLCKDRQIIKLRHTED
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCHH
FQEYRYASELYGDCKAVRNARIATVSRESEDIRAPAPYNLTELQKDANRYHNLTAIQVQD
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEHHH
ITQGLYEKKLISYPRTASRLLTRDVYDTLPAVMEKILSRKEFRQYAKMTDFAAPPSDISA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QETTDHHAIIVTGMPPGTLNKEEQLVYTLILGRTLEAFMPSCRVEYTTIDVVCAARKFSV
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHH
QTYRVLKKGWLSIFGREHLIARGGMPCSALPDFSPGEPVPVSGCNIVRKRTLPVSPYTDE
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCH
ELVGYMDRSGLGTVSTRTNIIRTLLERKYIRYSGKYVIPTPKGLFFYETVRGMKVADTSL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEHHHCCCEEECCCC
TSGWEAEVARIEQGKLTQEEFLGGVLEQVKEVTGEIFRIYQAKNNP
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA