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The map label for this gene is tyrS [H]

Identifier: 148828405

GI number: 148828405

Start: 1859057

End: 1860244

Strand: Direct

Name: tyrS [H]

Synonym: CGSHiGG_10070

Alternate gene names: 148828405

Gene position: 1859057-1860244 (Clockwise)

Preceding gene: 148828404

Following gene: 148828413

Centisome position: 98.51

GC content: 39.65

Gene sequence:

>1188_bases
ATGACTGACATTAATACCGTTCTCGCGGAACTAAAGCGTGGTACTGATGAAATCCTTTCAGAAGCGGATTTAATCGAAAA
ACTGAAAGAAAATCGCCCATTAAAAGTAAAATTAGGCGCAGATCCAACCGCACCAGATATTCATTTAGGACATACTGTTG
TATTAAACAAATTACGTCAATTTCAACAACTCGGTCATGAAGTCTATTTCTTAATTGGTGATTTCACTGGAATGGTGGGC
GATCCATCGGGCAAAAATGCCACACGTCCTCCACTTAGCCGTGAAGATGTATTGCGCAATGCGGAAACCTATAAAGAGCA
AATTTATAAAATTCTCGATCCACAAAAAACCAAAATCGTCTTCAACTCTGAATGGTTAAGCAAACTTGGTACAGAAGGAA
TGATTCGCCTTGCAAGCAACTATACGGTTGCACGTATGCTTGAACGCGACGACTTCAAAAAACGCTTTGGCAACAATCAA
CCCATTGCCATCCATGAATTTATTTATCCATTACTGCAAGGCTACGATTCTGTTGCATTAGATGCAGATGTGGAACTAGG
CGGTACAGACCAAAAATTCAACTTACTTGTTGGTCGTGAATTACAAAAATCAGCAGGCAAAAAACCACAGGTTGCGATTA
CACTCCCATTGCTCGTTGGCTTAGACGGCGAGAAAAAAATGTCCAAATCACTTGGTAACTATATTGGCGTAACGGAAGCA
CCAAGCGATATGTTCGGTAAAGTGATGTCAATTTCTGATGAACTCATGTGGGATTGGTATAACCTTCTTTCATTTCGTCC
ATTAAGCGAAATTGCACAATTAAAATCGGAAGTAGAAAACGGCAAAAATCCACGTGATGTAAAAATCTTATTAGCAAAAG
AATTAATTGCACGTTTCCACAATGAAGAGGCAGCAAACGCTGCAGAACAAGAATTTATTAACCGTTTCCAAAAAGGCGCA
ATGCCTGATGAAATGCCTGAATTTACGTTTTCTGGCGAAATTGGTTTAGCCACCTTATTAAAAGAAGCAGGTCTTGTTCC
TTCCACTTCTGAAGCGATTCGCTCTGCTCAACAAGGCGGTGTAAAAATCAATGGTGAAAAAGTCGATAACGTAAAAGATA
ATGCACCAAAAGGCACAAATGTTTACCAAGTGGGTAAACGAAAATTTGCGCGTGTAACGATTGAATAA

Upstream 100 bases:

>100_bases
CATTCCCGCCTATTTCCCCACAAATAGGCGTTTTTTTTATTCACAAATTGCTAAGAATCCCTATAATAAAACCTTTTAAA
ACATAATTTAGGAAATAATA

Downstream 100 bases:

>100_bases
AGTAGATACAGTTAAATAAAGTAAATAAAAAAATACCGCACTTTGGCAATAAAAGTGCGGTATTTTTTATTGAAATTTTA
ATTTAGTTAAACATCGCAGA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVYFLIGDFTGMVG
DPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIVFNSEWLSKLGTEGMIRLASNYTVARMLERDDFKKRFGNNQ
PIAIHEFIYPLLQGYDSVALDADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
PSDMFGKVMSISDELMWDWYNLLSFRPLSEIAQLKSEVENGKNPRDVKILLAKELIARFHNEEAANAAEQEFINRFQKGA
MPDEMPEFTFSGEIGLATLLKEAGLVPSTSEAIRSAQQGGVKINGEKVDNVKDNAPKGTNVYQVGKRKFARVTIE

Sequences:

>Translated_395_residues
MTDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVYFLIGDFTGMVG
DPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIVFNSEWLSKLGTEGMIRLASNYTVARMLERDDFKKRFGNNQ
PIAIHEFIYPLLQGYDSVALDADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
PSDMFGKVMSISDELMWDWYNLLSFRPLSEIAQLKSEVENGKNPRDVKILLAKELIARFHNEEAANAAEQEFINRFQKGA
MPDEMPEFTFSGEIGLATLLKEAGLVPSTSEAIRSAQQGGVKINGEKVDNVKDNAPKGTNVYQVGKRKFARVTIE
>Mature_394_residues
TDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVYFLIGDFTGMVGD
PSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIVFNSEWLSKLGTEGMIRLASNYTVARMLERDDFKKRFGNNQP
IAIHEFIYPLLQGYDSVALDADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEAP
SDMFGKVMSISDELMWDWYNLLSFRPLSEIAQLKSEVENGKNPRDVKILLAKELIARFHNEEAANAAEQEFINRFQKGAM
PDEMPEFTFSGEIGLATLLKEAGLVPSTSEAIRSAQQGGVKINGEKVDNVKDNAPKGTNVYQVGKRKFARVTIE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=408, Percent_Identity=25.4901960784314, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1787925, Length=376, Percent_Identity=28.4574468085106, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=223, Percent_Identity=31.8385650224215, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325160, Length=418, Percent_Identity=25.8373205741627, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI20130383, Length=400, Percent_Identity=27, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44038; Mature: 43907

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQ
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQQLGHEVYFLIGDFTGMVGDPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIV
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
FNSEWLSKLGTEGMIRLASNYTVARMLERDDFKKRFGNNQPIAIHEFIYPLLQGYDSVAL
ECHHHHHHCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEE
DADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
ECCEEECCCCCHHHHHHCHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCCC
PSDMFGKVMSISDELMWDWYNLLSFRPLSEIAQLKSEVENGKNPRDVKILLAKELIARFH
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
NEEAANAAEQEFINRFQKGAMPDEMPEFTFSGEIGLATLLKEAGLVPSTSEAIRSAQQGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
VKINGEKVDNVKDNAPKGTNVYQVGKRKFARVTIE
EEECCCCCCCCCCCCCCCCCHHHHCCHHHEEEECC
>Mature Secondary Structure 
TDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQ
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQQLGHEVYFLIGDFTGMVGDPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIV
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
FNSEWLSKLGTEGMIRLASNYTVARMLERDDFKKRFGNNQPIAIHEFIYPLLQGYDSVAL
ECHHHHHHCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEE
DADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
ECCEEECCCCCHHHHHHCHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCCC
PSDMFGKVMSISDELMWDWYNLLSFRPLSEIAQLKSEVENGKNPRDVKILLAKELIARFH
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
NEEAANAAEQEFINRFQKGAMPDEMPEFTFSGEIGLATLLKEAGLVPSTSEAIRSAQQGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
VKINGEKVDNVKDNAPKGTNVYQVGKRKFARVTIE
EEECCCCCCCCCCCCCCCCCHHHHCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]