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The map label for this gene is hmrM [H]

Identifier: 148828403

GI number: 148828403

Start: 1856740

End: 1858134

Strand: Direct

Name: hmrM [H]

Synonym: CGSHiGG_10060

Alternate gene names: 148828403

Gene position: 1856740-1858134 (Clockwise)

Preceding gene: 148828401

Following gene: 148828404

Centisome position: 98.39

GC content: 37.92

Gene sequence:

>1395_bases
ATGAATTTTCGTCTTTTATCTCAATACCACGCTGATATTAAAAATTTGATTAAAATTTCCTTGCCTATTTTATTAGCACA
AATCGCACAAAACTCAATGGGATTAGCGGATACCATTATGGCGGGTCGTGTGAGTTCCACTGATATGGCAGCCATTTCTA
TTGGTGCTTCAATTTGGATGCCATTGATGCTTTTTGGGCAAGGTTTATTGTTGGCATTGCCGCCTACAATTTCTTATTTG
AATGGTTCAGGCCAACGCCATCGCATTGCCCATCAAGTTCGCCAAGGCATTTGGCTTGTGTTAGGCGTGAGTATTCCTTT
AGGTTTGCTGATTTATTTCTGTGAAATTCCGCTGCAATATATGCAAATGGAAAGCAAAATGTCAGATTTAGCACGGGATT
ATTTACATGCGATGTTGTGGGGATTGCCAGCCTATTTGATGCTGATTAATTTTCGCTGTTTAAATGATGGTATTGCTAAA
ACTAAGCCTGCGATGGTCATTACCTTTTTAGGTTTGCTAATTAATATTCCACTTAATTACATTTTTATTTATGGAAAATT
TGGCATACCTGCTTTTGGTGCGGTGGGCTGTGGTATTGCAACAGCTATTGTGAACTGGGCAATGTGCTTAATGATGATTT
TTTATTCCTGTACTAATGCTCAAGAACGTTCACTAAAAGTATTTAGCCAATTAATCGAAATGCCAAATCTGAAAACACTT
AAAAAATTACTGCGTTTAGGATTACCCATTGCCATTGCAATTTGTTGCGAAGTGGCGTTATTTGCACTTACGTCTTTAAT
GCTTTCTCCGCTGGGTGCAACTATTGTCGCAAGCCATCAAATCACATTGAATACCAGTTCTTTTATTTTTATGTTCCCTA
TGTCGATTGGTATGGCAGCGACGATTCTAGTTGGACAAGCGTTGGGTACAGGTTCACCACAAAATGCGAAGAAAATTGGC
TATGCCGCATTATTATTAGGGCTAACGGTGACGATTATTACTGCATTAATTACGATTTTCTTCCGTTATGAAATTGCCTC
TATTTTCGTAACCGATGAAATTGTCATCGCAATGGCGGCAAATTTATTATTATTTGCCGCACTTTATCAATTTTCAGATA
CCATTCAAATGGTGGTTGGTGGCATTTTACGTGGCTATAAAGATACTAAAATGATTTTATACATTACCCTTTTTTCTTAT
TGGGTAATTGGTGTGCCACTTGGTTACACACTCGGTCGTACAGATTGGCTTATTCCACATATTGATGCGAAAGGTTTCTG
GATTGCCTTTGTGGTCTCACTCACTTTTGCGGCGTTCTTACTTTCTTTGAGAATGAAAAAAATGCAAGCCATGAGCGATA
ACGCCATTTTACAACGTTTAGAAAAACTTAAATAA

Upstream 100 bases:

>100_bases
AGCCTTTTCTTTAATGGAGTGGATGGGGGCGGTGCCTTGTACAATTCCAGTAAACATAATTTTTTCCTTTTCTAAAAATT
TTTAGCAGTATATCAAATTT

Downstream 100 bases:

>100_bases
GGAACTTTATGCAACTTCCAGCTTTACAATCGGCAAAATTAATTCGTCGTTACAAACGCTTTCTTGCGGATATTGAATTG
CCAACTGGTGATGTGATGAC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNFRLLSQYHADIKNLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWMPLMLFGQGLLLALPPTISYL
NGSGQRHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQYMQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIAK
TKPAMVITFLGLLINIPLNYIFIYGKFGIPAFGAVGCGIATAIVNWAMCLMMIFYSCTNAQERSLKVFSQLIEMPNLKTL
KKLLRLGLPIAIAICCEVALFALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMAATILVGQALGTGSPQNAKKIG
YAALLLGLTVTIITALITIFFRYEIASIFVTDEIVIAMAANLLLFAALYQFSDTIQMVVGGILRGYKDTKMILYITLFSY
WVIGVPLGYTLGRTDWLIPHIDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMSDNAILQRLEKLK

Sequences:

>Translated_464_residues
MNFRLLSQYHADIKNLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWMPLMLFGQGLLLALPPTISYL
NGSGQRHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQYMQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIAK
TKPAMVITFLGLLINIPLNYIFIYGKFGIPAFGAVGCGIATAIVNWAMCLMMIFYSCTNAQERSLKVFSQLIEMPNLKTL
KKLLRLGLPIAIAICCEVALFALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMAATILVGQALGTGSPQNAKKIG
YAALLLGLTVTIITALITIFFRYEIASIFVTDEIVIAMAANLLLFAALYQFSDTIQMVVGGILRGYKDTKMILYITLFSY
WVIGVPLGYTLGRTDWLIPHIDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMSDNAILQRLEKLK
>Mature_464_residues
MNFRLLSQYHADIKNLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWMPLMLFGQGLLLALPPTISYL
NGSGQRHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQYMQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIAK
TKPAMVITFLGLLINIPLNYIFIYGKFGIPAFGAVGCGIATAIVNWAMCLMMIFYSCTNAQERSLKVFSQLIEMPNLKTL
KKLLRLGLPIAIAICCEVALFALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMAATILVGQALGTGSPQNAKKIG
YAALLLGLTVTIITALITIFFRYEIASIFVTDEIVIAMAANLLLFAALYQFSDTIQMVVGGILRGYKDTKMILYITLFSY
WVIGVPLGYTLGRTDWLIPHIDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMSDNAILQRLEKLK

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to norfloxacin, acriflavine, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, daunomycin, berberine and sodium deoxycholate [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=452, Percent_Identity=21.9026548672566, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI153792564, Length=466, Percent_Identity=20.3862660944206, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI38261960, Length=235, Percent_Identity=21.7021276595745, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI48994890, Length=458, Percent_Identity=47.1615720524017, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI226510954, Length=421, Percent_Identity=22.8028503562945, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321821, Length=385, Percent_Identity=23.1168831168831, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320545, Length=430, Percent_Identity=20.9302325581395, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 51146; Mature: 51146

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRLLSQYHADIKNLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
PLMLFGQGLLLALPPTISYLNGSGQRHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQY
HHHHHCCCCEEECCCHHHHCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
MQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIAKTKPAMVITFLGLLINIPLNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCE
IFIYGKFGIPAFGAVGCGIATAIVNWAMCLMMIFYSCTNAQERSLKVFSQLIEMPNLKTL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
KKLLRLGLPIAIAICCEVALFALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMAA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEHHHHHHHH
TILVGQALGTGSPQNAKKIGYAALLLGLTVTIITALITIFFRYEIASIFVTDEIVIAMAA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLLFAALYQFSDTIQMVVGGILRGYKDTKMILYITLFSYWVIGVPLGYTLGRTDWLIPH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
IDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMSDNAILQRLEKLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MNFRLLSQYHADIKNLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
PLMLFGQGLLLALPPTISYLNGSGQRHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQY
HHHHHCCCCEEECCCHHHHCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
MQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIAKTKPAMVITFLGLLINIPLNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCE
IFIYGKFGIPAFGAVGCGIATAIVNWAMCLMMIFYSCTNAQERSLKVFSQLIEMPNLKTL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
KKLLRLGLPIAIAICCEVALFALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMAA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEHHHHHHHH
TILVGQALGTGSPQNAKKIGYAALLLGLTVTIITALITIFFRYEIASIFVTDEIVIAMAA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLLFAALYQFSDTIQMVVGGILRGYKDTKMILYITLFSYWVIGVPLGYTLGRTDWLIPH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
IDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMSDNAILQRLEKLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]